Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS21785 and ABZR87_RS21790 are separated by 73 nucleotides ABZR87_RS21790 and ABZR87_RS21795 are separated by 48 nucleotides ABZR87_RS21795 and ABZR87_RS21800 are separated by 94 nucleotides
ABZR87_RS21785: ABZR87_RS21785 - PLP-dependent aminotransferase family protein, at 1,272,539 to 1,273,957
_RS21785
ABZR87_RS21790: ABZR87_RS21790 - DUF3005 domain-containing protein, at 1,274,031 to 1,274,486
_RS21790
ABZR87_RS21795: ABZR87_RS21795 - NUDIX domain-containing protein, at 1,274,535 to 1,275,269
_RS21795
ABZR87_RS21800: ABZR87_RS21800 - SPFH domain-containing protein, at 1,275,364 to 1,276,407
_RS21800
Position (kb)
1274
1275
1276 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1273.539 kb on + strand, within ABZR87_RS21785 at 1273.539 kb on + strand, within ABZR87_RS21785 at 1273.539 kb on + strand, within ABZR87_RS21785 at 1273.539 kb on + strand, within ABZR87_RS21785 at 1273.539 kb on + strand, within ABZR87_RS21785 at 1273.539 kb on + strand, within ABZR87_RS21785 at 1273.540 kb on - strand, within ABZR87_RS21785 at 1273.540 kb on - strand, within ABZR87_RS21785 at 1273.540 kb on - strand, within ABZR87_RS21785 at 1273.540 kb on - strand, within ABZR87_RS21785 at 1273.631 kb on + strand, within ABZR87_RS21785 at 1273.640 kb on + strand, within ABZR87_RS21785 at 1273.640 kb on + strand, within ABZR87_RS21785 at 1273.640 kb on + strand, within ABZR87_RS21785 at 1273.641 kb on - strand, within ABZR87_RS21785 at 1273.641 kb on - strand, within ABZR87_RS21785 at 1273.641 kb on - strand, within ABZR87_RS21785 at 1273.641 kb on - strand, within ABZR87_RS21785 at 1273.641 kb on - strand, within ABZR87_RS21785 at 1273.641 kb on - strand, within ABZR87_RS21785 at 1273.641 kb on - strand, within ABZR87_RS21785 at 1273.641 kb on - strand, within ABZR87_RS21785 at 1273.836 kb on - strand at 1273.836 kb on - strand at 1273.836 kb on - strand at 1273.836 kb on - strand at 1273.836 kb on - strand at 1273.836 kb on - strand at 1273.887 kb on - strand at 1273.887 kb on - strand at 1273.960 kb on + strand at 1273.961 kb on - strand at 1273.961 kb on - strand at 1274.277 kb on + strand, within ABZR87_RS21790 at 1274.277 kb on + strand, within ABZR87_RS21790 at 1274.277 kb on + strand, within ABZR87_RS21790 at 1274.278 kb on - strand, within ABZR87_RS21790 at 1274.278 kb on - strand, within ABZR87_RS21790 at 1274.278 kb on - strand, within ABZR87_RS21790 at 1274.485 kb on - strand at 1274.630 kb on - strand, within ABZR87_RS21795 at 1274.732 kb on - strand, within ABZR87_RS21795 at 1274.794 kb on + strand, within ABZR87_RS21795 at 1274.795 kb on - strand, within ABZR87_RS21795 at 1274.795 kb on - strand, within ABZR87_RS21795 at 1274.795 kb on - strand, within ABZR87_RS21795 at 1274.795 kb on - strand, within ABZR87_RS21795 at 1274.959 kb on + strand, within ABZR87_RS21795 at 1274.959 kb on + strand, within ABZR87_RS21795 at 1274.959 kb on + strand, within ABZR87_RS21795 at 1274.960 kb on - strand, within ABZR87_RS21795 at 1274.960 kb on - strand, within ABZR87_RS21795 at 1275.203 kb on + strand at 1275.204 kb on - strand at 1275.303 kb on + strand at 1275.303 kb on + strand at 1275.431 kb on + strand at 1275.431 kb on + strand at 1275.431 kb on + strand at 1275.431 kb on + strand at 1275.432 kb on - strand at 1275.432 kb on - strand at 1275.463 kb on + strand at 1275.463 kb on + strand at 1275.580 kb on + strand, within ABZR87_RS21800 at 1275.649 kb on + strand, within ABZR87_RS21800 at 1275.650 kb on - strand, within ABZR87_RS21800 at 1275.934 kb on + strand, within ABZR87_RS21800 at 1275.935 kb on - strand, within ABZR87_RS21800 at 1275.935 kb on - strand, within ABZR87_RS21800 at 1275.935 kb on - strand, within ABZR87_RS21800 at 1276.186 kb on + strand, within ABZR87_RS21800 at 1276.187 kb on - strand, within ABZR87_RS21800
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,273,539 + ABZR87_RS21785 0.70 +0.1 1,273,539 + ABZR87_RS21785 0.70 +1.8 1,273,539 + ABZR87_RS21785 0.70 -1.9 1,273,539 + ABZR87_RS21785 0.70 -1.1 1,273,539 + ABZR87_RS21785 0.70 -0.1 1,273,539 + ABZR87_RS21785 0.70 +0.5 1,273,540 - ABZR87_RS21785 0.71 -1.2 1,273,540 - ABZR87_RS21785 0.71 -0.0 1,273,540 - ABZR87_RS21785 0.71 -0.5 1,273,540 - ABZR87_RS21785 0.71 -1.0 1,273,631 + ABZR87_RS21785 0.77 +0.3 1,273,640 + ABZR87_RS21785 0.78 -0.8 1,273,640 + ABZR87_RS21785 0.78 -0.3 1,273,640 + ABZR87_RS21785 0.78 +0.6 1,273,641 - ABZR87_RS21785 0.78 +0.6 1,273,641 - ABZR87_RS21785 0.78 +0.4 1,273,641 - ABZR87_RS21785 0.78 +1.0 1,273,641 - ABZR87_RS21785 0.78 -0.3 1,273,641 - ABZR87_RS21785 0.78 +0.1 1,273,641 - ABZR87_RS21785 0.78 +0.4 1,273,641 - ABZR87_RS21785 0.78 +1.0 1,273,641 - ABZR87_RS21785 0.78 +0.1 1,273,836 - +0.5 1,273,836 - +0.0 1,273,836 - +1.6 1,273,836 - -0.9 1,273,836 - +0.1 1,273,836 - -0.0 1,273,887 - +2.2 1,273,887 - +1.0 1,273,960 + -0.9 1,273,961 - -0.3 1,273,961 - +0.3 1,274,277 + ABZR87_RS21790 0.54 -0.0 1,274,277 + ABZR87_RS21790 0.54 +0.3 1,274,277 + ABZR87_RS21790 0.54 +0.4 1,274,278 - ABZR87_RS21790 0.54 -0.9 1,274,278 - ABZR87_RS21790 0.54 +0.4 1,274,278 - ABZR87_RS21790 0.54 +0.9 1,274,485 - +0.6 1,274,630 - ABZR87_RS21795 0.13 -0.2 1,274,732 - ABZR87_RS21795 0.27 -0.8 1,274,794 + ABZR87_RS21795 0.35 -0.7 1,274,795 - ABZR87_RS21795 0.35 -0.8 1,274,795 - ABZR87_RS21795 0.35 -2.1 1,274,795 - ABZR87_RS21795 0.35 +0.3 1,274,795 - ABZR87_RS21795 0.35 -1.6 1,274,959 + ABZR87_RS21795 0.58 -2.0 1,274,959 + ABZR87_RS21795 0.58 -0.1 1,274,959 + ABZR87_RS21795 0.58 +0.3 1,274,960 - ABZR87_RS21795 0.58 -1.4 1,274,960 - ABZR87_RS21795 0.58 -0.0 1,275,203 + -0.4 1,275,204 - +0.4 1,275,303 + +0.5 1,275,303 + -0.7 1,275,431 + +0.2 1,275,431 + -0.0 1,275,431 + +0.4 1,275,431 + +0.0 1,275,432 - +0.8 1,275,432 - +0.3 1,275,463 + +0.4 1,275,463 + +1.4 1,275,580 + ABZR87_RS21800 0.21 +0.1 1,275,649 + ABZR87_RS21800 0.27 +0.2 1,275,650 - ABZR87_RS21800 0.27 +0.3 1,275,934 + ABZR87_RS21800 0.55 +0.2 1,275,935 - ABZR87_RS21800 0.55 -0.0 1,275,935 - ABZR87_RS21800 0.55 -1.0 1,275,935 - ABZR87_RS21800 0.55 +0.0 1,276,186 + ABZR87_RS21800 0.79 +1.3 1,276,187 - ABZR87_RS21800 0.79 -0.1
Or see this region's nucleotide sequence