Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS21760 and ABZR87_RS21765 are separated by 698 nucleotides ABZR87_RS21765 and ABZR87_RS21770 overlap by 26 nucleotides ABZR87_RS21770 and ABZR87_RS21775 are separated by 81 nucleotides
ABZR87_RS21760: ABZR87_RS21760 - methionine ABC transporter permease, at 1,266,473 to 1,267,126
_RS21760
ABZR87_RS21765: ABZR87_RS21765 - family 2A encapsulin nanocompartment cargo protein cysteine desulfurase, at 1,267,825 to 1,269,693
_RS21765
ABZR87_RS21770: ABZR87_RS21770 - family 2A encapsulin nanocompartment shell protein, at 1,269,668 to 1,270,606
_RS21770
ABZR87_RS21775: ABZR87_RS21775 - serine O-acetyltransferase EpsC, at 1,270,688 to 1,271,722
_RS21775
Position (kb)
1267
1268
1269
1270 Strain fitness (log2 ratio)
-2
-1
0
1 at 1266.922 kb on + strand, within ABZR87_RS21760 at 1266.922 kb on + strand, within ABZR87_RS21760 at 1266.923 kb on - strand, within ABZR87_RS21760 at 1267.013 kb on + strand, within ABZR87_RS21760 at 1267.114 kb on + strand at 1267.114 kb on + strand at 1267.115 kb on - strand at 1267.160 kb on - strand at 1267.363 kb on + strand at 1267.364 kb on - strand at 1267.409 kb on - strand at 1267.409 kb on - strand at 1267.409 kb on - strand at 1267.532 kb on + strand at 1267.672 kb on + strand at 1267.672 kb on + strand at 1267.672 kb on + strand at 1267.887 kb on - strand at 1267.966 kb on + strand at 1268.108 kb on + strand, within ABZR87_RS21765 at 1268.108 kb on + strand, within ABZR87_RS21765 at 1268.109 kb on - strand, within ABZR87_RS21765 at 1268.109 kb on - strand, within ABZR87_RS21765 at 1268.109 kb on - strand, within ABZR87_RS21765 at 1268.129 kb on + strand, within ABZR87_RS21765 at 1268.130 kb on - strand, within ABZR87_RS21765 at 1268.243 kb on + strand, within ABZR87_RS21765 at 1268.243 kb on + strand, within ABZR87_RS21765 at 1268.243 kb on + strand, within ABZR87_RS21765 at 1268.331 kb on - strand, within ABZR87_RS21765 at 1268.331 kb on - strand, within ABZR87_RS21765 at 1268.382 kb on - strand, within ABZR87_RS21765 at 1268.834 kb on + strand, within ABZR87_RS21765 at 1268.834 kb on + strand, within ABZR87_RS21765 at 1268.834 kb on + strand, within ABZR87_RS21765 at 1268.906 kb on + strand, within ABZR87_RS21765 at 1268.944 kb on - strand, within ABZR87_RS21765 at 1268.944 kb on - strand, within ABZR87_RS21765 at 1269.076 kb on - strand, within ABZR87_RS21765 at 1269.131 kb on + strand, within ABZR87_RS21765 at 1269.132 kb on - strand, within ABZR87_RS21765 at 1269.132 kb on - strand, within ABZR87_RS21765 at 1269.350 kb on + strand, within ABZR87_RS21765 at 1269.350 kb on + strand, within ABZR87_RS21765 at 1269.350 kb on + strand, within ABZR87_RS21765 at 1269.350 kb on + strand, within ABZR87_RS21765 at 1269.351 kb on - strand, within ABZR87_RS21765 at 1269.351 kb on - strand, within ABZR87_RS21765 at 1269.351 kb on - strand, within ABZR87_RS21765 at 1269.351 kb on - strand, within ABZR87_RS21765 at 1269.351 kb on - strand, within ABZR87_RS21765 at 1269.354 kb on - strand, within ABZR87_RS21765 at 1269.669 kb on + strand at 1269.669 kb on + strand at 1269.670 kb on - strand at 1269.670 kb on - strand at 1269.670 kb on - strand at 1269.670 kb on - strand at 1269.675 kb on + strand at 1269.676 kb on - strand at 1269.676 kb on - strand at 1269.676 kb on - strand at 1269.676 kb on - strand at 1269.676 kb on - strand at 1269.676 kb on - strand at 1269.676 kb on - strand at 1269.676 kb on - strand at 1269.696 kb on + strand at 1269.696 kb on + strand at 1269.696 kb on + strand at 1269.697 kb on - strand at 1269.697 kb on - strand at 1269.762 kb on + strand, within ABZR87_RS21770 at 1269.762 kb on + strand, within ABZR87_RS21770 at 1269.762 kb on + strand, within ABZR87_RS21770 at 1269.762 kb on + strand, within ABZR87_RS21770 at 1269.762 kb on + strand, within ABZR87_RS21770 at 1269.778 kb on - strand, within ABZR87_RS21770 at 1269.858 kb on + strand, within ABZR87_RS21770 at 1270.281 kb on + strand, within ABZR87_RS21770 at 1270.282 kb on - strand, within ABZR87_RS21770 at 1270.282 kb on - strand, within ABZR87_RS21770 at 1270.300 kb on + strand, within ABZR87_RS21770 at 1270.300 kb on + strand, within ABZR87_RS21770 at 1270.301 kb on - strand, within ABZR87_RS21770 at 1270.301 kb on - strand, within ABZR87_RS21770 at 1270.301 kb on - strand, within ABZR87_RS21770 at 1270.301 kb on - strand, within ABZR87_RS21770 at 1270.338 kb on + strand, within ABZR87_RS21770 at 1270.338 kb on + strand, within ABZR87_RS21770 at 1270.339 kb on - strand, within ABZR87_RS21770 at 1270.359 kb on + strand, within ABZR87_RS21770 at 1270.624 kb on - strand at 1270.667 kb on - strand at 1270.667 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,266,922 + ABZR87_RS21760 0.69 -1.2 1,266,922 + ABZR87_RS21760 0.69 +0.0 1,266,923 - ABZR87_RS21760 0.69 -0.4 1,267,013 + ABZR87_RS21760 0.83 -0.5 1,267,114 + +0.5 1,267,114 + -0.1 1,267,115 - -0.6 1,267,160 - -0.6 1,267,363 + -2.4 1,267,364 - -2.0 1,267,409 - -1.1 1,267,409 - +0.1 1,267,409 - +0.8 1,267,532 + +0.5 1,267,672 + -2.2 1,267,672 + +0.3 1,267,672 + +0.1 1,267,887 - -0.2 1,267,966 + +0.5 1,268,108 + ABZR87_RS21765 0.15 -0.8 1,268,108 + ABZR87_RS21765 0.15 -1.0 1,268,109 - ABZR87_RS21765 0.15 -0.0 1,268,109 - ABZR87_RS21765 0.15 -0.3 1,268,109 - ABZR87_RS21765 0.15 -1.9 1,268,129 + ABZR87_RS21765 0.16 +0.4 1,268,130 - ABZR87_RS21765 0.16 +1.6 1,268,243 + ABZR87_RS21765 0.22 -0.8 1,268,243 + ABZR87_RS21765 0.22 +0.4 1,268,243 + ABZR87_RS21765 0.22 +0.6 1,268,331 - ABZR87_RS21765 0.27 -1.2 1,268,331 - ABZR87_RS21765 0.27 +0.5 1,268,382 - ABZR87_RS21765 0.30 -0.8 1,268,834 + ABZR87_RS21765 0.54 +0.2 1,268,834 + ABZR87_RS21765 0.54 -1.6 1,268,834 + ABZR87_RS21765 0.54 -0.1 1,268,906 + ABZR87_RS21765 0.58 -0.7 1,268,944 - ABZR87_RS21765 0.60 -0.5 1,268,944 - ABZR87_RS21765 0.60 -1.0 1,269,076 - ABZR87_RS21765 0.67 -1.5 1,269,131 + ABZR87_RS21765 0.70 +1.1 1,269,132 - ABZR87_RS21765 0.70 -1.0 1,269,132 - ABZR87_RS21765 0.70 -0.6 1,269,350 + ABZR87_RS21765 0.82 +0.4 1,269,350 + ABZR87_RS21765 0.82 -0.4 1,269,350 + ABZR87_RS21765 0.82 -0.5 1,269,350 + ABZR87_RS21765 0.82 +0.5 1,269,351 - ABZR87_RS21765 0.82 -1.6 1,269,351 - ABZR87_RS21765 0.82 +0.7 1,269,351 - ABZR87_RS21765 0.82 +0.2 1,269,351 - ABZR87_RS21765 0.82 -0.5 1,269,351 - ABZR87_RS21765 0.82 +0.7 1,269,354 - ABZR87_RS21765 0.82 -0.2 1,269,669 + -0.0 1,269,669 + +1.1 1,269,670 - +0.5 1,269,670 - -0.4 1,269,670 - -0.9 1,269,670 - +0.1 1,269,675 + -0.8 1,269,676 - -1.0 1,269,676 - +0.6 1,269,676 - -1.1 1,269,676 - -0.5 1,269,676 - -2.3 1,269,676 - +0.1 1,269,676 - +0.1 1,269,676 - -1.7 1,269,696 + -0.1 1,269,696 + -0.2 1,269,696 + +0.1 1,269,697 - +0.7 1,269,697 - +0.1 1,269,762 + ABZR87_RS21770 0.10 -1.1 1,269,762 + ABZR87_RS21770 0.10 +0.8 1,269,762 + ABZR87_RS21770 0.10 -0.4 1,269,762 + ABZR87_RS21770 0.10 -0.2 1,269,762 + ABZR87_RS21770 0.10 +1.1 1,269,778 - ABZR87_RS21770 0.12 +1.7 1,269,858 + ABZR87_RS21770 0.20 -2.0 1,270,281 + ABZR87_RS21770 0.65 +0.3 1,270,282 - ABZR87_RS21770 0.65 -0.2 1,270,282 - ABZR87_RS21770 0.65 -0.7 1,270,300 + ABZR87_RS21770 0.67 -0.4 1,270,300 + ABZR87_RS21770 0.67 +1.1 1,270,301 - ABZR87_RS21770 0.67 -0.5 1,270,301 - ABZR87_RS21770 0.67 +0.5 1,270,301 - ABZR87_RS21770 0.67 -1.7 1,270,301 - ABZR87_RS21770 0.67 -1.4 1,270,338 + ABZR87_RS21770 0.71 -0.3 1,270,338 + ABZR87_RS21770 0.71 +0.3 1,270,339 - ABZR87_RS21770 0.71 -0.0 1,270,359 + ABZR87_RS21770 0.74 -0.8 1,270,624 - -0.7 1,270,667 - +0.3 1,270,667 - -0.6
Or see this region's nucleotide sequence