Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS20825 and ABZR87_RS20830 overlap by 4 nucleotides ABZR87_RS20830 and ABZR87_RS20835 are separated by 38 nucleotides ABZR87_RS20835 and ABZR87_RS20840 are separated by 3 nucleotides
ABZR87_RS20825: ABZR87_RS20825 - nuclear transport factor 2 family protein, at 1,058,003 to 1,058,461
_RS20825
ABZR87_RS20830: ABZR87_RS20830 - SDR family oxidoreductase, at 1,058,458 to 1,059,216
_RS20830
ABZR87_RS20835: ABZR87_RS20835 - ureidoglycolate lyase, at 1,059,255 to 1,060,103
_RS20835
ABZR87_RS20840: ABZR87_RS20840 - LysR family transcriptional regulator, at 1,060,107 to 1,061,024
_RS20840
Position (kb)
1059
1060
1061 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1058.442 kb on - strand at 1058.442 kb on - strand at 1058.442 kb on - strand at 1058.718 kb on - strand, within ABZR87_RS20830 at 1058.718 kb on - strand, within ABZR87_RS20830 at 1058.791 kb on + strand, within ABZR87_RS20830 at 1058.792 kb on - strand, within ABZR87_RS20830 at 1058.792 kb on - strand, within ABZR87_RS20830 at 1058.902 kb on + strand, within ABZR87_RS20830 at 1058.903 kb on - strand, within ABZR87_RS20830 at 1059.006 kb on - strand, within ABZR87_RS20830 at 1059.145 kb on + strand at 1059.146 kb on - strand at 1059.218 kb on - strand at 1059.492 kb on + strand, within ABZR87_RS20835 at 1059.493 kb on - strand, within ABZR87_RS20835 at 1059.663 kb on + strand, within ABZR87_RS20835 at 1059.663 kb on + strand, within ABZR87_RS20835 at 1059.663 kb on + strand, within ABZR87_RS20835 at 1059.664 kb on - strand, within ABZR87_RS20835 at 1059.664 kb on - strand, within ABZR87_RS20835 at 1059.691 kb on - strand, within ABZR87_RS20835 at 1059.691 kb on - strand, within ABZR87_RS20835 at 1059.691 kb on - strand, within ABZR87_RS20835 at 1059.702 kb on + strand, within ABZR87_RS20835 at 1059.890 kb on + strand, within ABZR87_RS20835 at 1059.930 kb on + strand, within ABZR87_RS20835 at 1059.930 kb on + strand, within ABZR87_RS20835 at 1059.930 kb on + strand, within ABZR87_RS20835 at 1059.931 kb on - strand, within ABZR87_RS20835 at 1059.931 kb on - strand, within ABZR87_RS20835 at 1060.023 kb on + strand at 1060.024 kb on - strand at 1060.024 kb on - strand at 1060.108 kb on + strand at 1060.109 kb on - strand at 1060.318 kb on + strand, within ABZR87_RS20840 at 1060.318 kb on + strand, within ABZR87_RS20840 at 1060.318 kb on + strand, within ABZR87_RS20840 at 1060.402 kb on + strand, within ABZR87_RS20840 at 1060.403 kb on - strand, within ABZR87_RS20840 at 1060.403 kb on - strand, within ABZR87_RS20840 at 1060.403 kb on - strand, within ABZR87_RS20840 at 1060.403 kb on - strand, within ABZR87_RS20840 at 1060.507 kb on + strand, within ABZR87_RS20840 at 1060.508 kb on - strand, within ABZR87_RS20840 at 1060.635 kb on - strand, within ABZR87_RS20840 at 1060.668 kb on + strand, within ABZR87_RS20840 at 1060.669 kb on - strand, within ABZR87_RS20840 at 1060.669 kb on - strand, within ABZR87_RS20840 at 1060.669 kb on - strand, within ABZR87_RS20840 at 1060.669 kb on - strand, within ABZR87_RS20840 at 1060.693 kb on + strand, within ABZR87_RS20840 at 1060.693 kb on + strand, within ABZR87_RS20840 at 1060.693 kb on + strand, within ABZR87_RS20840 at 1060.693 kb on + strand, within ABZR87_RS20840 at 1060.694 kb on - strand, within ABZR87_RS20840 at 1060.694 kb on - strand, within ABZR87_RS20840 at 1060.694 kb on - strand, within ABZR87_RS20840 at 1060.912 kb on - strand, within ABZR87_RS20840 at 1061.103 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,058,442 - +0.8 1,058,442 - -1.2 1,058,442 - +0.2 1,058,718 - ABZR87_RS20830 0.34 +0.6 1,058,718 - ABZR87_RS20830 0.34 +0.2 1,058,791 + ABZR87_RS20830 0.44 -0.5 1,058,792 - ABZR87_RS20830 0.44 -0.2 1,058,792 - ABZR87_RS20830 0.44 +0.4 1,058,902 + ABZR87_RS20830 0.58 +0.2 1,058,903 - ABZR87_RS20830 0.59 +0.1 1,059,006 - ABZR87_RS20830 0.72 -0.1 1,059,145 + +0.4 1,059,146 - -0.8 1,059,218 - -0.2 1,059,492 + ABZR87_RS20835 0.28 +3.3 1,059,493 - ABZR87_RS20835 0.28 -0.2 1,059,663 + ABZR87_RS20835 0.48 +0.7 1,059,663 + ABZR87_RS20835 0.48 -0.7 1,059,663 + ABZR87_RS20835 0.48 -0.1 1,059,664 - ABZR87_RS20835 0.48 +0.6 1,059,664 - ABZR87_RS20835 0.48 +0.6 1,059,691 - ABZR87_RS20835 0.51 -0.5 1,059,691 - ABZR87_RS20835 0.51 +0.3 1,059,691 - ABZR87_RS20835 0.51 +1.6 1,059,702 + ABZR87_RS20835 0.53 +1.0 1,059,890 + ABZR87_RS20835 0.75 -2.1 1,059,930 + ABZR87_RS20835 0.80 -2.1 1,059,930 + ABZR87_RS20835 0.80 -0.0 1,059,930 + ABZR87_RS20835 0.80 +0.3 1,059,931 - ABZR87_RS20835 0.80 +0.2 1,059,931 - ABZR87_RS20835 0.80 +1.3 1,060,023 + -0.3 1,060,024 - +0.1 1,060,024 - -0.5 1,060,108 + +0.3 1,060,109 - +0.6 1,060,318 + ABZR87_RS20840 0.23 -2.1 1,060,318 + ABZR87_RS20840 0.23 +0.2 1,060,318 + ABZR87_RS20840 0.23 -0.9 1,060,402 + ABZR87_RS20840 0.32 -1.7 1,060,403 - ABZR87_RS20840 0.32 +1.3 1,060,403 - ABZR87_RS20840 0.32 -1.0 1,060,403 - ABZR87_RS20840 0.32 -0.4 1,060,403 - ABZR87_RS20840 0.32 -0.5 1,060,507 + ABZR87_RS20840 0.44 +0.5 1,060,508 - ABZR87_RS20840 0.44 +2.0 1,060,635 - ABZR87_RS20840 0.58 -0.3 1,060,668 + ABZR87_RS20840 0.61 -0.5 1,060,669 - ABZR87_RS20840 0.61 -1.4 1,060,669 - ABZR87_RS20840 0.61 -0.3 1,060,669 - ABZR87_RS20840 0.61 -1.3 1,060,669 - ABZR87_RS20840 0.61 +0.6 1,060,693 + ABZR87_RS20840 0.64 -0.3 1,060,693 + ABZR87_RS20840 0.64 -0.1 1,060,693 + ABZR87_RS20840 0.64 -0.4 1,060,693 + ABZR87_RS20840 0.64 -2.4 1,060,694 - ABZR87_RS20840 0.64 -0.3 1,060,694 - ABZR87_RS20840 0.64 -0.9 1,060,694 - ABZR87_RS20840 0.64 -1.3 1,060,912 - ABZR87_RS20840 0.88 +0.1 1,061,103 + -1.9
Or see this region's nucleotide sequence