Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS20815 and ABZR87_RS20820 are separated by 278 nucleotides ABZR87_RS20820 and ABZR87_RS20825 are separated by 46 nucleotides ABZR87_RS20825 and ABZR87_RS20830 overlap by 4 nucleotides
ABZR87_RS20815: ABZR87_RS20815 - 5-dehydro-4-deoxy-D-glucuronate isomerase, at 1,055,825 to 1,056,661
_RS20815
ABZR87_RS20820: ABZR87_RS20820 - 2-keto-3-deoxygluconate transporter, at 1,056,940 to 1,057,956
_RS20820
ABZR87_RS20825: ABZR87_RS20825 - nuclear transport factor 2 family protein, at 1,058,003 to 1,058,461
_RS20825
ABZR87_RS20830: ABZR87_RS20830 - SDR family oxidoreductase, at 1,058,458 to 1,059,216
_RS20830
Position (kb)
1056
1057
1058 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1056.043 kb on - strand, within ABZR87_RS20815 at 1056.048 kb on + strand, within ABZR87_RS20815 at 1056.048 kb on + strand, within ABZR87_RS20815 at 1056.048 kb on + strand, within ABZR87_RS20815 at 1056.048 kb on + strand, within ABZR87_RS20815 at 1056.049 kb on - strand, within ABZR87_RS20815 at 1056.049 kb on - strand, within ABZR87_RS20815 at 1056.171 kb on + strand, within ABZR87_RS20815 at 1056.171 kb on + strand, within ABZR87_RS20815 at 1056.253 kb on - strand, within ABZR87_RS20815 at 1056.283 kb on - strand, within ABZR87_RS20815 at 1056.283 kb on - strand, within ABZR87_RS20815 at 1056.283 kb on - strand, within ABZR87_RS20815 at 1056.283 kb on - strand, within ABZR87_RS20815 at 1056.348 kb on + strand, within ABZR87_RS20815 at 1056.348 kb on + strand, within ABZR87_RS20815 at 1056.348 kb on + strand, within ABZR87_RS20815 at 1056.349 kb on - strand, within ABZR87_RS20815 at 1056.546 kb on + strand, within ABZR87_RS20815 at 1056.546 kb on + strand, within ABZR87_RS20815 at 1056.546 kb on + strand, within ABZR87_RS20815 at 1056.547 kb on - strand, within ABZR87_RS20815 at 1056.547 kb on - strand, within ABZR87_RS20815 at 1056.547 kb on - strand, within ABZR87_RS20815 at 1056.878 kb on + strand at 1056.921 kb on - strand at 1056.921 kb on - strand at 1056.921 kb on - strand at 1057.017 kb on + strand at 1057.017 kb on + strand at 1057.017 kb on + strand at 1057.018 kb on - strand at 1057.018 kb on - strand at 1057.018 kb on - strand at 1057.018 kb on - strand at 1057.042 kb on + strand, within ABZR87_RS20820 at 1057.042 kb on + strand, within ABZR87_RS20820 at 1057.315 kb on + strand, within ABZR87_RS20820 at 1057.316 kb on - strand, within ABZR87_RS20820 at 1057.336 kb on + strand, within ABZR87_RS20820 at 1057.485 kb on + strand, within ABZR87_RS20820 at 1057.486 kb on - strand, within ABZR87_RS20820 at 1057.486 kb on - strand, within ABZR87_RS20820 at 1058.192 kb on + strand, within ABZR87_RS20825 at 1058.192 kb on + strand, within ABZR87_RS20825 at 1058.192 kb on + strand, within ABZR87_RS20825 at 1058.192 kb on + strand, within ABZR87_RS20825 at 1058.193 kb on - strand, within ABZR87_RS20825 at 1058.193 kb on - strand, within ABZR87_RS20825 at 1058.442 kb on - strand at 1058.442 kb on - strand at 1058.442 kb on - strand at 1058.718 kb on - strand, within ABZR87_RS20830 at 1058.718 kb on - strand, within ABZR87_RS20830 at 1058.791 kb on + strand, within ABZR87_RS20830 at 1058.792 kb on - strand, within ABZR87_RS20830 at 1058.792 kb on - strand, within ABZR87_RS20830 at 1058.902 kb on + strand, within ABZR87_RS20830 at 1058.903 kb on - strand, within ABZR87_RS20830
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,056,043 - ABZR87_RS20815 0.26 -0.6 1,056,048 + ABZR87_RS20815 0.27 +0.8 1,056,048 + ABZR87_RS20815 0.27 +0.1 1,056,048 + ABZR87_RS20815 0.27 -0.3 1,056,048 + ABZR87_RS20815 0.27 -0.3 1,056,049 - ABZR87_RS20815 0.27 +2.1 1,056,049 - ABZR87_RS20815 0.27 +0.2 1,056,171 + ABZR87_RS20815 0.41 -0.5 1,056,171 + ABZR87_RS20815 0.41 -0.9 1,056,253 - ABZR87_RS20815 0.51 +0.6 1,056,283 - ABZR87_RS20815 0.55 -0.3 1,056,283 - ABZR87_RS20815 0.55 +0.0 1,056,283 - ABZR87_RS20815 0.55 +0.5 1,056,283 - ABZR87_RS20815 0.55 -0.5 1,056,348 + ABZR87_RS20815 0.62 -0.4 1,056,348 + ABZR87_RS20815 0.62 +0.8 1,056,348 + ABZR87_RS20815 0.62 -1.0 1,056,349 - ABZR87_RS20815 0.63 +0.2 1,056,546 + ABZR87_RS20815 0.86 +0.4 1,056,546 + ABZR87_RS20815 0.86 +0.7 1,056,546 + ABZR87_RS20815 0.86 +0.8 1,056,547 - ABZR87_RS20815 0.86 -0.3 1,056,547 - ABZR87_RS20815 0.86 -0.0 1,056,547 - ABZR87_RS20815 0.86 +0.8 1,056,878 + +0.1 1,056,921 - -1.0 1,056,921 - -0.6 1,056,921 - +0.0 1,057,017 + +0.4 1,057,017 + -0.7 1,057,017 + +0.2 1,057,018 - -2.8 1,057,018 - +0.4 1,057,018 - -2.8 1,057,018 - -0.3 1,057,042 + ABZR87_RS20820 0.10 -0.1 1,057,042 + ABZR87_RS20820 0.10 -0.9 1,057,315 + ABZR87_RS20820 0.37 -0.2 1,057,316 - ABZR87_RS20820 0.37 -1.4 1,057,336 + ABZR87_RS20820 0.39 -0.8 1,057,485 + ABZR87_RS20820 0.54 -0.6 1,057,486 - ABZR87_RS20820 0.54 +0.6 1,057,486 - ABZR87_RS20820 0.54 -0.3 1,058,192 + ABZR87_RS20825 0.41 +1.0 1,058,192 + ABZR87_RS20825 0.41 -0.3 1,058,192 + ABZR87_RS20825 0.41 -1.1 1,058,192 + ABZR87_RS20825 0.41 +0.6 1,058,193 - ABZR87_RS20825 0.41 +0.3 1,058,193 - ABZR87_RS20825 0.41 -1.9 1,058,442 - +0.8 1,058,442 - -1.2 1,058,442 - +0.2 1,058,718 - ABZR87_RS20830 0.34 +0.6 1,058,718 - ABZR87_RS20830 0.34 +0.2 1,058,791 + ABZR87_RS20830 0.44 -0.5 1,058,792 - ABZR87_RS20830 0.44 -0.2 1,058,792 - ABZR87_RS20830 0.44 +0.4 1,058,902 + ABZR87_RS20830 0.58 +0.2 1,058,903 - ABZR87_RS20830 0.59 +0.1
Or see this region's nucleotide sequence