Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS20655 and ABZR87_RS20660 overlap by 8 nucleotides ABZR87_RS20660 and ABZR87_RS20665 are separated by 2 nucleotides ABZR87_RS20665 and ABZR87_RS20670 are separated by 12 nucleotides ABZR87_RS20670 and ABZR87_RS20675 are separated by 4 nucleotides
ABZR87_RS20655: ABZR87_RS20655 - thiamine pyrophosphate-binding protein, at 1,022,205 to 1,023,932
_RS20655
ABZR87_RS20660: ABZR87_RS20660 - SDR family oxidoreductase, at 1,023,925 to 1,024,728
_RS20660
ABZR87_RS20665: ABZR87_RS20665 - IclR family transcriptional regulator, at 1,024,731 to 1,025,558
_RS20665
ABZR87_RS20670: ABZR87_RS20670 - alpha/beta hydrolase, at 1,025,571 to 1,026,473
_RS20670
ABZR87_RS20675: ABZR87_RS20675 - cupin domain-containing protein, at 1,026,478 to 1,027,014
_RS20675
Position (kb)
1024
1025
1026 Strain fitness (log2 ratio)
-2
-1
0
1 at 1023.927 kb on - strand at 1023.954 kb on + strand at 1023.954 kb on + strand at 1023.955 kb on - strand at 1023.955 kb on - strand at 1023.977 kb on + strand at 1024.693 kb on + strand at 1024.694 kb on - strand at 1024.786 kb on + strand at 1024.786 kb on + strand at 1024.964 kb on - strand, within ABZR87_RS20665 at 1024.964 kb on - strand, within ABZR87_RS20665 at 1024.964 kb on - strand, within ABZR87_RS20665 at 1025.057 kb on - strand, within ABZR87_RS20665 at 1025.188 kb on + strand, within ABZR87_RS20665 at 1025.300 kb on - strand, within ABZR87_RS20665 at 1025.518 kb on + strand at 1025.519 kb on - strand at 1025.519 kb on - strand at 1025.558 kb on + strand at 1025.558 kb on + strand at 1025.558 kb on + strand at 1025.559 kb on - strand at 1025.559 kb on - strand at 1025.639 kb on + strand at 1025.639 kb on + strand at 1025.639 kb on + strand at 1025.640 kb on - strand at 1025.640 kb on - strand at 1025.641 kb on + strand at 1025.641 kb on + strand at 1025.642 kb on - strand at 1025.642 kb on - strand at 1025.724 kb on - strand, within ABZR87_RS20670 at 1025.785 kb on + strand, within ABZR87_RS20670 at 1025.785 kb on + strand, within ABZR87_RS20670 at 1025.785 kb on + strand, within ABZR87_RS20670 at 1025.786 kb on - strand, within ABZR87_RS20670 at 1026.079 kb on + strand, within ABZR87_RS20670 at 1026.080 kb on - strand, within ABZR87_RS20670 at 1026.080 kb on - strand, within ABZR87_RS20670 at 1026.173 kb on - strand, within ABZR87_RS20670 at 1026.221 kb on - strand, within ABZR87_RS20670 at 1026.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,023,927 - +0.4 1,023,954 + -0.7 1,023,954 + +0.5 1,023,955 - -0.1 1,023,955 - +0.1 1,023,977 + +0.7 1,024,693 + +0.2 1,024,694 - -0.2 1,024,786 + +0.3 1,024,786 + -0.4 1,024,964 - ABZR87_RS20665 0.28 -0.7 1,024,964 - ABZR87_RS20665 0.28 -1.1 1,024,964 - ABZR87_RS20665 0.28 -1.0 1,025,057 - ABZR87_RS20665 0.39 -2.0 1,025,188 + ABZR87_RS20665 0.55 +0.3 1,025,300 - ABZR87_RS20665 0.69 +0.0 1,025,518 + +0.5 1,025,519 - +0.1 1,025,519 - -0.9 1,025,558 + -0.6 1,025,558 + -1.4 1,025,558 + +0.5 1,025,559 - -0.4 1,025,559 - +0.2 1,025,639 + +0.5 1,025,639 + +0.3 1,025,639 + +0.1 1,025,640 - +0.7 1,025,640 - -0.4 1,025,641 + +0.4 1,025,641 + -1.5 1,025,642 - -0.6 1,025,642 - +0.2 1,025,724 - ABZR87_RS20670 0.17 -0.7 1,025,785 + ABZR87_RS20670 0.24 -2.0 1,025,785 + ABZR87_RS20670 0.24 -1.8 1,025,785 + ABZR87_RS20670 0.24 -0.2 1,025,786 - ABZR87_RS20670 0.24 +0.6 1,026,079 + ABZR87_RS20670 0.56 -1.3 1,026,080 - ABZR87_RS20670 0.56 +1.0 1,026,080 - ABZR87_RS20670 0.56 +1.2 1,026,173 - ABZR87_RS20670 0.67 +1.1 1,026,221 - ABZR87_RS20670 0.72 +0.3 1,026,516 - -0.6
Or see this region's nucleotide sequence