Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS20380 and ABZR87_RS20385 are separated by 58 nucleotides ABZR87_RS20385 and ABZR87_RS20390 are separated by 9 nucleotides ABZR87_RS20390 and ABZR87_RS20395 are separated by 197 nucleotides
ABZR87_RS20380: ABZR87_RS20380 - ABC transporter substrate-binding protein, at 960,348 to 961,355
_RS20380
ABZR87_RS20385: ABZR87_RS20385 - class II aldolase/adducin family protein, at 961,414 to 962,253
_RS20385
ABZR87_RS20390: ABZR87_RS20390 - LLM class flavin-dependent oxidoreductase, at 962,263 to 963,354
_RS20390
ABZR87_RS20395: ABZR87_RS20395 - acyl-CoA dehydrogenase family protein, at 963,552 to 964,760
_RS20395
Position (kb) 962
963
964 Strain fitness (log2 ratio) -2
-1
0
1
2 at 961.317 kb on + strand at 961.318 kb on - strand at 961.383 kb on - strand at 961.416 kb on - strand at 961.454 kb on + strand at 961.509 kb on + strand, within ABZR87_RS20385 at 961.509 kb on + strand, within ABZR87_RS20385 at 961.509 kb on + strand, within ABZR87_RS20385 at 961.598 kb on + strand, within ABZR87_RS20385 at 961.598 kb on + strand, within ABZR87_RS20385 at 961.599 kb on - strand, within ABZR87_RS20385 at 961.599 kb on - strand, within ABZR87_RS20385 at 961.599 kb on - strand, within ABZR87_RS20385 at 961.599 kb on - strand, within ABZR87_RS20385 at 961.772 kb on + strand, within ABZR87_RS20385 at 961.772 kb on + strand, within ABZR87_RS20385 at 961.773 kb on - strand, within ABZR87_RS20385 at 961.838 kb on + strand, within ABZR87_RS20385 at 961.839 kb on - strand, within ABZR87_RS20385 at 961.851 kb on + strand, within ABZR87_RS20385 at 961.852 kb on - strand, within ABZR87_RS20385 at 962.396 kb on + strand, within ABZR87_RS20390 at 962.396 kb on + strand, within ABZR87_RS20390 at 962.396 kb on + strand, within ABZR87_RS20390 at 962.396 kb on + strand, within ABZR87_RS20390 at 962.397 kb on - strand, within ABZR87_RS20390 at 962.399 kb on + strand, within ABZR87_RS20390 at 962.771 kb on + strand, within ABZR87_RS20390 at 962.771 kb on + strand, within ABZR87_RS20390 at 962.807 kb on - strand, within ABZR87_RS20390 at 962.828 kb on + strand, within ABZR87_RS20390 at 962.829 kb on - strand, within ABZR87_RS20390 at 962.888 kb on + strand, within ABZR87_RS20390 at 962.889 kb on - strand, within ABZR87_RS20390 at 962.889 kb on - strand, within ABZR87_RS20390 at 962.889 kb on - strand, within ABZR87_RS20390 at 962.889 kb on - strand, within ABZR87_RS20390 at 962.951 kb on + strand, within ABZR87_RS20390 at 962.952 kb on - strand, within ABZR87_RS20390 at 963.165 kb on - strand, within ABZR87_RS20390 at 963.165 kb on - strand, within ABZR87_RS20390 at 963.259 kb on + strand at 963.259 kb on + strand at 963.260 kb on - strand at 963.260 kb on - strand at 963.263 kb on + strand at 963.378 kb on + strand at 963.470 kb on + strand at 963.471 kb on - strand at 963.471 kb on - strand at 963.472 kb on + strand at 963.473 kb on - strand at 963.473 kb on - strand at 963.473 kb on - strand at 963.479 kb on + strand at 963.479 kb on + strand at 963.480 kb on - strand at 963.831 kb on + strand, within ABZR87_RS20395 at 963.831 kb on + strand, within ABZR87_RS20395 at 963.832 kb on - strand, within ABZR87_RS20395 at 963.864 kb on - strand, within ABZR87_RS20395 at 964.039 kb on - strand, within ABZR87_RS20395
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 961,317 + +0.9 961,318 - +0.0 961,383 - -0.0 961,416 - +1.5 961,454 + -0.0 961,509 + ABZR87_RS20385 0.11 -1.0 961,509 + ABZR87_RS20385 0.11 -0.2 961,509 + ABZR87_RS20385 0.11 -2.3 961,598 + ABZR87_RS20385 0.22 -1.1 961,598 + ABZR87_RS20385 0.22 -1.1 961,599 - ABZR87_RS20385 0.22 -0.5 961,599 - ABZR87_RS20385 0.22 -0.1 961,599 - ABZR87_RS20385 0.22 -0.7 961,599 - ABZR87_RS20385 0.22 +0.8 961,772 + ABZR87_RS20385 0.43 +0.1 961,772 + ABZR87_RS20385 0.43 -1.0 961,773 - ABZR87_RS20385 0.43 +0.6 961,838 + ABZR87_RS20385 0.50 -0.9 961,839 - ABZR87_RS20385 0.51 +0.0 961,851 + ABZR87_RS20385 0.52 +1.7 961,852 - ABZR87_RS20385 0.52 -0.2 962,396 + ABZR87_RS20390 0.12 +0.5 962,396 + ABZR87_RS20390 0.12 +0.0 962,396 + ABZR87_RS20390 0.12 +0.7 962,396 + ABZR87_RS20390 0.12 -0.8 962,397 - ABZR87_RS20390 0.12 +2.0 962,399 + ABZR87_RS20390 0.12 +0.9 962,771 + ABZR87_RS20390 0.47 +0.8 962,771 + ABZR87_RS20390 0.47 -0.1 962,807 - ABZR87_RS20390 0.50 -1.3 962,828 + ABZR87_RS20390 0.52 +0.8 962,829 - ABZR87_RS20390 0.52 +0.5 962,888 + ABZR87_RS20390 0.57 +0.4 962,889 - ABZR87_RS20390 0.57 +0.4 962,889 - ABZR87_RS20390 0.57 +0.1 962,889 - ABZR87_RS20390 0.57 -0.1 962,889 - ABZR87_RS20390 0.57 -0.7 962,951 + ABZR87_RS20390 0.63 +1.0 962,952 - ABZR87_RS20390 0.63 +1.0 963,165 - ABZR87_RS20390 0.83 +0.4 963,165 - ABZR87_RS20390 0.83 +0.2 963,259 + +0.4 963,259 + -0.5 963,260 - -0.2 963,260 - -0.3 963,263 + -0.3 963,378 + -0.2 963,470 + -0.1 963,471 - +1.0 963,471 - -1.0 963,472 + +0.4 963,473 - -0.2 963,473 - +0.1 963,473 - -0.2 963,479 + +0.3 963,479 + +0.4 963,480 - +0.1 963,831 + ABZR87_RS20395 0.23 -1.4 963,831 + ABZR87_RS20395 0.23 -0.5 963,832 - ABZR87_RS20395 0.23 -1.1 963,864 - ABZR87_RS20395 0.26 +0.4 964,039 - ABZR87_RS20395 0.40 -2.2
Or see this region's nucleotide sequence