Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS20050 and ABZR87_RS20055 are separated by 51 nucleotides ABZR87_RS20055 and ABZR87_RS20060 are separated by 38 nucleotides ABZR87_RS20060 and ABZR87_RS20065 are separated by 38 nucleotides ABZR87_RS20065 and ABZR87_RS20070 are separated by 287 nucleotides
ABZR87_RS20050: ABZR87_RS20050 - flavodoxin family protein, at 875,288 to 875,818
_RS20050
ABZR87_RS20055: ABZR87_RS20055 - BON domain-containing protein, at 875,870 to 876,523
_RS20055
ABZR87_RS20060: ABZR87_RS20060 - universal stress protein, at 876,562 to 877,401
_RS20060
ABZR87_RS20065: ABZR87_RS20065 - universal stress protein, at 877,440 to 877,940
_RS20065
ABZR87_RS20070: ABZR87_RS20070 - CBS domain-containing protein, at 878,228 to 878,695
_RS20070
Position (kb)
876
877
878 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 875.594 kb on + strand, within ABZR87_RS20050 at 875.634 kb on + strand, within ABZR87_RS20050 at 875.635 kb on - strand, within ABZR87_RS20050 at 875.635 kb on - strand, within ABZR87_RS20050 at 875.635 kb on - strand, within ABZR87_RS20050 at 875.635 kb on - strand, within ABZR87_RS20050 at 875.639 kb on - strand, within ABZR87_RS20050 at 875.660 kb on + strand, within ABZR87_RS20050 at 875.660 kb on + strand, within ABZR87_RS20050 at 875.793 kb on + strand at 875.793 kb on + strand at 875.796 kb on + strand at 875.796 kb on + strand at 875.796 kb on + strand at 875.796 kb on + strand at 875.796 kb on + strand at 875.797 kb on - strand at 875.797 kb on - strand at 875.823 kb on + strand at 875.824 kb on - strand at 875.824 kb on - strand at 875.871 kb on + strand at 875.872 kb on - strand at 875.872 kb on - strand at 875.872 kb on - strand at 875.973 kb on + strand, within ABZR87_RS20055 at 875.973 kb on + strand, within ABZR87_RS20055 at 875.974 kb on - strand, within ABZR87_RS20055 at 876.242 kb on + strand, within ABZR87_RS20055 at 876.243 kb on - strand, within ABZR87_RS20055 at 876.243 kb on - strand, within ABZR87_RS20055 at 876.243 kb on - strand, within ABZR87_RS20055 at 876.243 kb on - strand, within ABZR87_RS20055 at 876.243 kb on - strand, within ABZR87_RS20055 at 876.397 kb on + strand, within ABZR87_RS20055 at 876.397 kb on + strand, within ABZR87_RS20055 at 876.825 kb on + strand, within ABZR87_RS20060 at 876.825 kb on + strand, within ABZR87_RS20060 at 876.825 kb on + strand, within ABZR87_RS20060 at 876.826 kb on - strand, within ABZR87_RS20060 at 876.968 kb on + strand, within ABZR87_RS20060 at 876.968 kb on + strand, within ABZR87_RS20060 at 876.969 kb on - strand, within ABZR87_RS20060 at 877.186 kb on + strand, within ABZR87_RS20060 at 877.186 kb on + strand, within ABZR87_RS20060 at 877.186 kb on + strand, within ABZR87_RS20060 at 877.187 kb on - strand, within ABZR87_RS20060 at 877.187 kb on - strand, within ABZR87_RS20060 at 877.187 kb on - strand, within ABZR87_RS20060 at 877.233 kb on - strand, within ABZR87_RS20060 at 877.233 kb on - strand, within ABZR87_RS20060 at 877.256 kb on + strand, within ABZR87_RS20060 at 877.256 kb on + strand, within ABZR87_RS20060 at 877.257 kb on - strand, within ABZR87_RS20060 at 877.257 kb on - strand, within ABZR87_RS20060 at 877.257 kb on - strand, within ABZR87_RS20060 at 877.289 kb on + strand, within ABZR87_RS20060 at 877.289 kb on + strand, within ABZR87_RS20060 at 877.289 kb on + strand, within ABZR87_RS20060 at 877.289 kb on + strand, within ABZR87_RS20060 at 877.289 kb on + strand, within ABZR87_RS20060 at 877.290 kb on - strand, within ABZR87_RS20060 at 877.290 kb on - strand, within ABZR87_RS20060 at 877.325 kb on + strand at 877.325 kb on + strand at 877.326 kb on - strand at 877.326 kb on - strand at 877.394 kb on + strand at 877.441 kb on + strand at 877.442 kb on - strand at 877.442 kb on - strand at 877.772 kb on + strand, within ABZR87_RS20065 at 877.792 kb on + strand, within ABZR87_RS20065 at 877.792 kb on + strand, within ABZR87_RS20065 at 877.793 kb on - strand, within ABZR87_RS20065 at 877.793 kb on - strand, within ABZR87_RS20065 at 877.804 kb on + strand, within ABZR87_RS20065 at 877.804 kb on + strand, within ABZR87_RS20065 at 877.804 kb on + strand, within ABZR87_RS20065 at 877.805 kb on - strand, within ABZR87_RS20065 at 877.805 kb on - strand, within ABZR87_RS20065 at 877.813 kb on + strand, within ABZR87_RS20065 at 877.813 kb on + strand, within ABZR87_RS20065 at 877.813 kb on + strand, within ABZR87_RS20065 at 877.814 kb on - strand, within ABZR87_RS20065 at 877.814 kb on - strand, within ABZR87_RS20065 at 877.936 kb on + strand at 877.936 kb on + strand at 877.936 kb on + strand at 877.936 kb on + strand at 877.937 kb on - strand at 877.937 kb on - strand at 877.937 kb on - strand at 877.937 kb on - strand at 877.937 kb on - strand at 877.937 kb on - strand at 877.937 kb on - strand at 877.937 kb on - strand at 877.937 kb on - strand at 877.937 kb on - strand at 878.026 kb on + strand at 878.026 kb on + strand at 878.026 kb on + strand at 878.027 kb on - strand at 878.027 kb on - strand at 878.027 kb on - strand at 878.027 kb on - strand at 878.027 kb on - strand at 878.103 kb on - strand at 878.103 kb on - strand at 878.195 kb on + strand at 878.195 kb on + strand at 878.195 kb on + strand at 878.195 kb on + strand at 878.195 kb on + strand at 878.196 kb on - strand at 878.196 kb on - strand at 878.196 kb on - strand at 878.196 kb on - strand at 878.197 kb on + strand at 878.198 kb on - strand at 878.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 875,594 + ABZR87_RS20050 0.58 -0.8 875,634 + ABZR87_RS20050 0.65 +0.6 875,635 - ABZR87_RS20050 0.65 +0.4 875,635 - ABZR87_RS20050 0.65 -2.0 875,635 - ABZR87_RS20050 0.65 +0.3 875,635 - ABZR87_RS20050 0.65 +2.0 875,639 - ABZR87_RS20050 0.66 +0.7 875,660 + ABZR87_RS20050 0.70 -0.5 875,660 + ABZR87_RS20050 0.70 +0.1 875,793 + -1.4 875,793 + -0.6 875,796 + +0.9 875,796 + -0.8 875,796 + +0.3 875,796 + -0.8 875,796 + -0.1 875,797 - +0.2 875,797 - -0.5 875,823 + +0.2 875,824 - +1.7 875,824 - +0.7 875,871 + -0.2 875,872 - +0.4 875,872 - -0.4 875,872 - -0.0 875,973 + ABZR87_RS20055 0.16 +1.5 875,973 + ABZR87_RS20055 0.16 +0.9 875,974 - ABZR87_RS20055 0.16 -0.0 876,242 + ABZR87_RS20055 0.57 +0.3 876,243 - ABZR87_RS20055 0.57 -0.1 876,243 - ABZR87_RS20055 0.57 -2.0 876,243 - ABZR87_RS20055 0.57 -0.1 876,243 - ABZR87_RS20055 0.57 +1.4 876,243 - ABZR87_RS20055 0.57 -1.6 876,397 + ABZR87_RS20055 0.81 +2.0 876,397 + ABZR87_RS20055 0.81 +0.6 876,825 + ABZR87_RS20060 0.31 +0.5 876,825 + ABZR87_RS20060 0.31 -1.7 876,825 + ABZR87_RS20060 0.31 -0.2 876,826 - ABZR87_RS20060 0.31 -0.7 876,968 + ABZR87_RS20060 0.48 -0.4 876,968 + ABZR87_RS20060 0.48 -0.0 876,969 - ABZR87_RS20060 0.48 +0.3 877,186 + ABZR87_RS20060 0.74 -1.8 877,186 + ABZR87_RS20060 0.74 -0.6 877,186 + ABZR87_RS20060 0.74 -0.2 877,187 - ABZR87_RS20060 0.74 +0.4 877,187 - ABZR87_RS20060 0.74 -0.4 877,187 - ABZR87_RS20060 0.74 +1.5 877,233 - ABZR87_RS20060 0.80 +0.0 877,233 - ABZR87_RS20060 0.80 -1.3 877,256 + ABZR87_RS20060 0.83 -0.4 877,256 + ABZR87_RS20060 0.83 +0.2 877,257 - ABZR87_RS20060 0.83 -0.5 877,257 - ABZR87_RS20060 0.83 -0.1 877,257 - ABZR87_RS20060 0.83 -1.4 877,289 + ABZR87_RS20060 0.87 -0.2 877,289 + ABZR87_RS20060 0.87 -1.0 877,289 + ABZR87_RS20060 0.87 -0.4 877,289 + ABZR87_RS20060 0.87 -1.1 877,289 + ABZR87_RS20060 0.87 +1.0 877,290 - ABZR87_RS20060 0.87 +0.5 877,290 - ABZR87_RS20060 0.87 +0.4 877,325 + -1.0 877,325 + +0.5 877,326 - -1.8 877,326 - -1.0 877,394 + +0.7 877,441 + +0.6 877,442 - -0.4 877,442 - -0.1 877,772 + ABZR87_RS20065 0.66 -2.5 877,792 + ABZR87_RS20065 0.70 -0.4 877,792 + ABZR87_RS20065 0.70 -0.5 877,793 - ABZR87_RS20065 0.70 +0.1 877,793 - ABZR87_RS20065 0.70 -0.1 877,804 + ABZR87_RS20065 0.73 -0.2 877,804 + ABZR87_RS20065 0.73 -0.6 877,804 + ABZR87_RS20065 0.73 -0.9 877,805 - ABZR87_RS20065 0.73 -1.3 877,805 - ABZR87_RS20065 0.73 -0.0 877,813 + ABZR87_RS20065 0.74 +0.4 877,813 + ABZR87_RS20065 0.74 -0.6 877,813 + ABZR87_RS20065 0.74 +0.2 877,814 - ABZR87_RS20065 0.75 +0.8 877,814 - ABZR87_RS20065 0.75 +0.7 877,936 + -0.4 877,936 + -0.0 877,936 + +1.0 877,936 + -0.4 877,937 - -0.0 877,937 - +0.5 877,937 - -2.4 877,937 - +0.4 877,937 - +0.5 877,937 - +0.0 877,937 - -0.3 877,937 - -0.3 877,937 - -1.1 877,937 - +0.6 878,026 + +0.9 878,026 + -0.4 878,026 + -3.2 878,027 - +0.6 878,027 - +0.9 878,027 - -0.7 878,027 - -1.0 878,027 - +0.2 878,103 - +0.4 878,103 - -0.6 878,195 + -1.1 878,195 + +0.3 878,195 + -1.1 878,195 + -0.2 878,195 + -0.0 878,196 - +0.2 878,196 - -0.0 878,196 - +0.8 878,196 - -0.4 878,197 + +0.2 878,198 - -0.1 878,209 - -0.2
Or see this region's nucleotide sequence