Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS19705 and ABZR87_RS19710 overlap by 18 nucleotides ABZR87_RS19710 and ABZR87_RS19715 are separated by 153 nucleotides ABZR87_RS19715 and ABZR87_RS19720 are separated by 2 nucleotides
ABZR87_RS19705: ABZR87_RS19705 - APC family permease, at 796,529 to 797,911
_RS19705
ABZR87_RS19710: ABZR87_RS19710 - Hpt domain-containing protein, at 797,894 to 798,307
_RS19710
ABZR87_RS19715: ABZR87_RS19715 - CusA/CzcA family heavy metal efflux RND transporter, at 798,461 to 801,556
_RS19715
ABZR87_RS19720: ABZR87_RS19720 - efflux RND transporter periplasmic adaptor subunit, at 801,559 to 802,707
_RS19720
Position (kb)
798
799
800
801
802 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 797.462 kb on + strand, within ABZR87_RS19705 at 797.462 kb on + strand, within ABZR87_RS19705 at 797.463 kb on + strand, within ABZR87_RS19705 at 797.463 kb on - strand, within ABZR87_RS19705 at 797.498 kb on + strand, within ABZR87_RS19705 at 797.498 kb on + strand, within ABZR87_RS19705 at 797.654 kb on + strand, within ABZR87_RS19705 at 797.654 kb on + strand, within ABZR87_RS19705 at 797.654 kb on + strand, within ABZR87_RS19705 at 797.654 kb on + strand, within ABZR87_RS19705 at 797.655 kb on - strand, within ABZR87_RS19705 at 797.655 kb on - strand, within ABZR87_RS19705 at 797.715 kb on + strand, within ABZR87_RS19705 at 797.783 kb on + strand at 797.783 kb on + strand at 798.274 kb on + strand at 798.765 kb on + strand at 798.765 kb on + strand at 798.765 kb on + strand at 798.766 kb on - strand at 798.766 kb on - strand at 798.942 kb on + strand, within ABZR87_RS19715 at 799.044 kb on + strand, within ABZR87_RS19715 at 799.045 kb on - strand, within ABZR87_RS19715 at 799.061 kb on + strand, within ABZR87_RS19715 at 799.062 kb on - strand, within ABZR87_RS19715 at 799.062 kb on - strand, within ABZR87_RS19715 at 799.062 kb on - strand, within ABZR87_RS19715 at 799.143 kb on + strand, within ABZR87_RS19715 at 799.143 kb on + strand, within ABZR87_RS19715 at 799.144 kb on - strand, within ABZR87_RS19715 at 799.266 kb on + strand, within ABZR87_RS19715 at 799.267 kb on - strand, within ABZR87_RS19715 at 799.359 kb on + strand, within ABZR87_RS19715 at 799.360 kb on - strand, within ABZR87_RS19715 at 799.360 kb on - strand, within ABZR87_RS19715 at 799.510 kb on - strand, within ABZR87_RS19715 at 799.611 kb on + strand, within ABZR87_RS19715 at 799.612 kb on - strand, within ABZR87_RS19715 at 799.612 kb on - strand, within ABZR87_RS19715 at 799.612 kb on - strand, within ABZR87_RS19715 at 799.612 kb on - strand, within ABZR87_RS19715 at 799.626 kb on + strand, within ABZR87_RS19715 at 799.657 kb on + strand, within ABZR87_RS19715 at 799.657 kb on + strand, within ABZR87_RS19715 at 799.657 kb on + strand, within ABZR87_RS19715 at 799.657 kb on + strand, within ABZR87_RS19715 at 799.657 kb on + strand, within ABZR87_RS19715 at 799.657 kb on + strand, within ABZR87_RS19715 at 799.657 kb on + strand, within ABZR87_RS19715 at 799.658 kb on - strand, within ABZR87_RS19715 at 799.658 kb on - strand, within ABZR87_RS19715 at 799.658 kb on - strand, within ABZR87_RS19715 at 799.658 kb on - strand, within ABZR87_RS19715 at 799.661 kb on + strand, within ABZR87_RS19715 at 799.662 kb on - strand, within ABZR87_RS19715 at 799.662 kb on - strand, within ABZR87_RS19715 at 799.662 kb on - strand, within ABZR87_RS19715 at 799.722 kb on + strand, within ABZR87_RS19715 at 799.723 kb on - strand, within ABZR87_RS19715 at 799.723 kb on - strand, within ABZR87_RS19715 at 799.723 kb on - strand, within ABZR87_RS19715 at 799.723 kb on - strand, within ABZR87_RS19715 at 799.723 kb on - strand, within ABZR87_RS19715 at 799.825 kb on - strand, within ABZR87_RS19715 at 799.873 kb on + strand, within ABZR87_RS19715 at 799.874 kb on - strand, within ABZR87_RS19715 at 799.874 kb on - strand, within ABZR87_RS19715 at 799.874 kb on - strand, within ABZR87_RS19715 at 799.874 kb on - strand, within ABZR87_RS19715 at 799.886 kb on - strand, within ABZR87_RS19715 at 799.953 kb on + strand, within ABZR87_RS19715 at 799.953 kb on + strand, within ABZR87_RS19715 at 800.034 kb on + strand, within ABZR87_RS19715 at 800.035 kb on - strand, within ABZR87_RS19715 at 800.035 kb on - strand, within ABZR87_RS19715 at 800.595 kb on + strand, within ABZR87_RS19715 at 800.595 kb on + strand, within ABZR87_RS19715 at 800.595 kb on + strand, within ABZR87_RS19715 at 800.595 kb on + strand, within ABZR87_RS19715 at 800.596 kb on - strand, within ABZR87_RS19715 at 800.596 kb on - strand, within ABZR87_RS19715 at 800.596 kb on - strand, within ABZR87_RS19715 at 800.739 kb on + strand, within ABZR87_RS19715 at 800.739 kb on + strand, within ABZR87_RS19715 at 800.739 kb on + strand, within ABZR87_RS19715 at 800.739 kb on + strand, within ABZR87_RS19715 at 800.739 kb on + strand, within ABZR87_RS19715 at 800.740 kb on - strand, within ABZR87_RS19715 at 800.740 kb on - strand, within ABZR87_RS19715 at 800.740 kb on - strand, within ABZR87_RS19715 at 800.740 kb on - strand, within ABZR87_RS19715 at 800.740 kb on - strand, within ABZR87_RS19715 at 800.740 kb on - strand, within ABZR87_RS19715 at 800.740 kb on - strand, within ABZR87_RS19715 at 800.740 kb on - strand, within ABZR87_RS19715 at 800.740 kb on - strand, within ABZR87_RS19715 at 800.740 kb on - strand, within ABZR87_RS19715 at 800.973 kb on + strand, within ABZR87_RS19715 at 800.974 kb on - strand, within ABZR87_RS19715 at 801.010 kb on - strand, within ABZR87_RS19715 at 801.010 kb on - strand, within ABZR87_RS19715 at 801.010 kb on - strand, within ABZR87_RS19715 at 801.084 kb on + strand, within ABZR87_RS19715 at 801.084 kb on + strand, within ABZR87_RS19715 at 801.085 kb on - strand, within ABZR87_RS19715 at 801.085 kb on - strand, within ABZR87_RS19715 at 801.219 kb on + strand, within ABZR87_RS19715 at 801.219 kb on + strand, within ABZR87_RS19715 at 801.220 kb on - strand, within ABZR87_RS19715 at 801.220 kb on - strand, within ABZR87_RS19715 at 801.250 kb on + strand at 801.251 kb on - strand at 801.251 kb on - strand at 801.442 kb on - strand at 801.560 kb on + strand at 801.560 kb on + strand at 801.560 kb on + strand at 801.560 kb on + strand at 801.608 kb on - strand at 801.731 kb on + strand, within ABZR87_RS19720 at 801.812 kb on + strand, within ABZR87_RS19720 at 801.812 kb on + strand, within ABZR87_RS19720 at 801.812 kb on + strand, within ABZR87_RS19720 at 801.813 kb on - strand, within ABZR87_RS19720 at 801.813 kb on - strand, within ABZR87_RS19720 at 801.813 kb on - strand, within ABZR87_RS19720 at 801.860 kb on - strand, within ABZR87_RS19720 at 801.995 kb on + strand, within ABZR87_RS19720 at 801.995 kb on + strand, within ABZR87_RS19720 at 801.995 kb on + strand, within ABZR87_RS19720 at 801.995 kb on + strand, within ABZR87_RS19720 at 801.996 kb on - strand, within ABZR87_RS19720 at 801.996 kb on - strand, within ABZR87_RS19720 at 802.292 kb on + strand, within ABZR87_RS19720 at 802.325 kb on + strand, within ABZR87_RS19720 at 802.325 kb on + strand, within ABZR87_RS19720 at 802.437 kb on - strand, within ABZR87_RS19720 at 802.437 kb on - strand, within ABZR87_RS19720
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 797,462 + ABZR87_RS19705 0.67 +1.4 797,462 + ABZR87_RS19705 0.67 +1.0 797,463 + ABZR87_RS19705 0.68 +1.2 797,463 - ABZR87_RS19705 0.68 +0.4 797,498 + ABZR87_RS19705 0.70 -0.0 797,498 + ABZR87_RS19705 0.70 -0.0 797,654 + ABZR87_RS19705 0.81 +0.3 797,654 + ABZR87_RS19705 0.81 -0.3 797,654 + ABZR87_RS19705 0.81 +1.3 797,654 + ABZR87_RS19705 0.81 +0.8 797,655 - ABZR87_RS19705 0.81 +0.5 797,655 - ABZR87_RS19705 0.81 +0.2 797,715 + ABZR87_RS19705 0.86 +0.3 797,783 + +0.7 797,783 + -0.3 798,274 + +1.3 798,765 + -0.7 798,765 + -0.7 798,765 + -0.1 798,766 - -0.3 798,766 - +3.0 798,942 + ABZR87_RS19715 0.16 -2.7 799,044 + ABZR87_RS19715 0.19 +0.0 799,045 - ABZR87_RS19715 0.19 -0.6 799,061 + ABZR87_RS19715 0.19 -0.2 799,062 - ABZR87_RS19715 0.19 -0.8 799,062 - ABZR87_RS19715 0.19 -1.4 799,062 - ABZR87_RS19715 0.19 -0.0 799,143 + ABZR87_RS19715 0.22 -1.4 799,143 + ABZR87_RS19715 0.22 -2.3 799,144 - ABZR87_RS19715 0.22 -1.4 799,266 + ABZR87_RS19715 0.26 -1.4 799,267 - ABZR87_RS19715 0.26 -0.7 799,359 + ABZR87_RS19715 0.29 -0.5 799,360 - ABZR87_RS19715 0.29 -0.7 799,360 - ABZR87_RS19715 0.29 -1.3 799,510 - ABZR87_RS19715 0.34 -2.0 799,611 + ABZR87_RS19715 0.37 -0.8 799,612 - ABZR87_RS19715 0.37 +0.5 799,612 - ABZR87_RS19715 0.37 -0.5 799,612 - ABZR87_RS19715 0.37 -0.4 799,612 - ABZR87_RS19715 0.37 +0.0 799,626 + ABZR87_RS19715 0.38 -0.1 799,657 + ABZR87_RS19715 0.39 -0.5 799,657 + ABZR87_RS19715 0.39 -0.4 799,657 + ABZR87_RS19715 0.39 -2.3 799,657 + ABZR87_RS19715 0.39 -0.2 799,657 + ABZR87_RS19715 0.39 -1.4 799,657 + ABZR87_RS19715 0.39 -0.8 799,657 + ABZR87_RS19715 0.39 -0.2 799,658 - ABZR87_RS19715 0.39 -0.6 799,658 - ABZR87_RS19715 0.39 -3.1 799,658 - ABZR87_RS19715 0.39 -2.0 799,658 - ABZR87_RS19715 0.39 -1.1 799,661 + ABZR87_RS19715 0.39 -0.3 799,662 - ABZR87_RS19715 0.39 -0.8 799,662 - ABZR87_RS19715 0.39 -1.4 799,662 - ABZR87_RS19715 0.39 -1.8 799,722 + ABZR87_RS19715 0.41 -1.3 799,723 - ABZR87_RS19715 0.41 +0.5 799,723 - ABZR87_RS19715 0.41 -3.0 799,723 - ABZR87_RS19715 0.41 -0.9 799,723 - ABZR87_RS19715 0.41 -1.4 799,723 - ABZR87_RS19715 0.41 +0.2 799,825 - ABZR87_RS19715 0.44 -0.6 799,873 + ABZR87_RS19715 0.46 -0.6 799,874 - ABZR87_RS19715 0.46 -0.4 799,874 - ABZR87_RS19715 0.46 -0.8 799,874 - ABZR87_RS19715 0.46 -0.4 799,874 - ABZR87_RS19715 0.46 +0.4 799,886 - ABZR87_RS19715 0.46 -0.4 799,953 + ABZR87_RS19715 0.48 +0.5 799,953 + ABZR87_RS19715 0.48 +0.6 800,034 + ABZR87_RS19715 0.51 -1.0 800,035 - ABZR87_RS19715 0.51 -1.4 800,035 - ABZR87_RS19715 0.51 -0.1 800,595 + ABZR87_RS19715 0.69 +0.1 800,595 + ABZR87_RS19715 0.69 -0.5 800,595 + ABZR87_RS19715 0.69 -0.7 800,595 + ABZR87_RS19715 0.69 -0.7 800,596 - ABZR87_RS19715 0.69 -1.2 800,596 - ABZR87_RS19715 0.69 -1.8 800,596 - ABZR87_RS19715 0.69 -0.5 800,739 + ABZR87_RS19715 0.74 -0.3 800,739 + ABZR87_RS19715 0.74 -0.7 800,739 + ABZR87_RS19715 0.74 -0.2 800,739 + ABZR87_RS19715 0.74 -0.4 800,739 + ABZR87_RS19715 0.74 -1.3 800,740 - ABZR87_RS19715 0.74 -2.3 800,740 - ABZR87_RS19715 0.74 +0.2 800,740 - ABZR87_RS19715 0.74 -0.2 800,740 - ABZR87_RS19715 0.74 -0.8 800,740 - ABZR87_RS19715 0.74 -1.0 800,740 - ABZR87_RS19715 0.74 -1.1 800,740 - ABZR87_RS19715 0.74 +0.0 800,740 - ABZR87_RS19715 0.74 -0.2 800,740 - ABZR87_RS19715 0.74 -0.5 800,740 - ABZR87_RS19715 0.74 -1.5 800,973 + ABZR87_RS19715 0.81 +0.2 800,974 - ABZR87_RS19715 0.81 -3.1 801,010 - ABZR87_RS19715 0.82 -0.1 801,010 - ABZR87_RS19715 0.82 -1.0 801,010 - ABZR87_RS19715 0.82 -0.5 801,084 + ABZR87_RS19715 0.85 -0.6 801,084 + ABZR87_RS19715 0.85 -1.9 801,085 - ABZR87_RS19715 0.85 +0.2 801,085 - ABZR87_RS19715 0.85 -0.8 801,219 + ABZR87_RS19715 0.89 -0.9 801,219 + ABZR87_RS19715 0.89 -0.7 801,220 - ABZR87_RS19715 0.89 -1.5 801,220 - ABZR87_RS19715 0.89 -2.3 801,250 + -2.3 801,251 - -0.0 801,251 - -0.3 801,442 - -2.6 801,560 + +0.9 801,560 + +0.4 801,560 + -0.1 801,560 + -0.2 801,608 - -1.2 801,731 + ABZR87_RS19720 0.15 +0.1 801,812 + ABZR87_RS19720 0.22 +0.0 801,812 + ABZR87_RS19720 0.22 -1.4 801,812 + ABZR87_RS19720 0.22 -1.0 801,813 - ABZR87_RS19720 0.22 -0.7 801,813 - ABZR87_RS19720 0.22 -1.5 801,813 - ABZR87_RS19720 0.22 +0.4 801,860 - ABZR87_RS19720 0.26 -0.4 801,995 + ABZR87_RS19720 0.38 +0.4 801,995 + ABZR87_RS19720 0.38 -1.1 801,995 + ABZR87_RS19720 0.38 -2.5 801,995 + ABZR87_RS19720 0.38 -1.8 801,996 - ABZR87_RS19720 0.38 -1.4 801,996 - ABZR87_RS19720 0.38 -0.1 802,292 + ABZR87_RS19720 0.64 -1.3 802,325 + ABZR87_RS19720 0.67 -0.4 802,325 + ABZR87_RS19720 0.67 +0.7 802,437 - ABZR87_RS19720 0.76 -1.5 802,437 - ABZR87_RS19720 0.76 -1.9
Or see this region's nucleotide sequence