Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS19580 and ABZR87_RS19585 are separated by 150 nucleotides ABZR87_RS19585 and ABZR87_RS19590 are separated by 170 nucleotides ABZR87_RS19590 and ABZR87_RS19595 are separated by 59 nucleotides
ABZR87_RS19580: ABZR87_RS19580 - DUF2968 domain-containing protein, at 764,993 to 765,784
_RS19580
ABZR87_RS19585: ABZR87_RS19585 - cytochrome P450, at 765,935 to 769,174
_RS19585
ABZR87_RS19590: ABZR87_RS19590 - hypothetical protein, at 769,345 to 769,635
_RS19590
ABZR87_RS19595: ABZR87_RS19595 - Gfo/Idh/MocA family oxidoreductase, at 769,695 to 770,849
_RS19595
Position (kb) 765
766
767
768
769
770 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 764.948 kb on + strand at 764.949 kb on - strand at 764.949 kb on - strand at 764.949 kb on - strand at 764.950 kb on + strand at 764.951 kb on - strand at 764.951 kb on - strand at 764.951 kb on - strand at 764.951 kb on - strand at 765.083 kb on - strand, within ABZR87_RS19580 at 765.279 kb on + strand, within ABZR87_RS19580 at 765.335 kb on + strand, within ABZR87_RS19580 at 765.335 kb on + strand, within ABZR87_RS19580 at 765.335 kb on + strand, within ABZR87_RS19580 at 765.335 kb on + strand, within ABZR87_RS19580 at 765.335 kb on + strand, within ABZR87_RS19580 at 765.336 kb on - strand, within ABZR87_RS19580 at 765.336 kb on - strand, within ABZR87_RS19580 at 765.336 kb on - strand, within ABZR87_RS19580 at 765.336 kb on - strand, within ABZR87_RS19580 at 765.336 kb on - strand, within ABZR87_RS19580 at 765.336 kb on - strand, within ABZR87_RS19580 at 765.336 kb on - strand, within ABZR87_RS19580 at 765.347 kb on + strand, within ABZR87_RS19580 at 765.347 kb on + strand, within ABZR87_RS19580 at 765.347 kb on + strand, within ABZR87_RS19580 at 765.386 kb on + strand, within ABZR87_RS19580 at 765.386 kb on + strand, within ABZR87_RS19580 at 765.386 kb on + strand, within ABZR87_RS19580 at 765.386 kb on + strand, within ABZR87_RS19580 at 765.387 kb on - strand, within ABZR87_RS19580 at 765.387 kb on - strand, within ABZR87_RS19580 at 765.387 kb on - strand, within ABZR87_RS19580 at 765.387 kb on - strand, within ABZR87_RS19580 at 765.387 kb on - strand, within ABZR87_RS19580 at 765.387 kb on - strand, within ABZR87_RS19580 at 765.387 kb on - strand, within ABZR87_RS19580 at 765.389 kb on + strand, within ABZR87_RS19580 at 765.389 kb on + strand, within ABZR87_RS19580 at 765.389 kb on + strand, within ABZR87_RS19580 at 765.389 kb on + strand, within ABZR87_RS19580 at 765.389 kb on + strand, within ABZR87_RS19580 at 765.389 kb on + strand, within ABZR87_RS19580 at 765.389 kb on + strand, within ABZR87_RS19580 at 765.389 kb on + strand, within ABZR87_RS19580 at 765.390 kb on - strand, within ABZR87_RS19580 at 765.390 kb on - strand, within ABZR87_RS19580 at 765.390 kb on - strand, within ABZR87_RS19580 at 765.390 kb on - strand, within ABZR87_RS19580 at 765.464 kb on + strand, within ABZR87_RS19580 at 765.464 kb on + strand, within ABZR87_RS19580 at 765.465 kb on - strand, within ABZR87_RS19580 at 765.465 kb on - strand, within ABZR87_RS19580 at 765.605 kb on - strand, within ABZR87_RS19580 at 765.843 kb on - strand at 765.897 kb on + strand at 765.897 kb on + strand at 765.897 kb on + strand at 765.897 kb on + strand at 765.897 kb on + strand at 765.897 kb on + strand at 765.898 kb on - strand at 765.898 kb on - strand at 765.898 kb on - strand at 766.000 kb on + strand at 766.001 kb on - strand at 766.001 kb on - strand at 766.001 kb on - strand at 766.097 kb on + strand at 766.136 kb on + strand at 766.136 kb on + strand at 766.137 kb on - strand at 766.322 kb on + strand, within ABZR87_RS19585 at 766.323 kb on - strand, within ABZR87_RS19585 at 766.477 kb on + strand, within ABZR87_RS19585 at 766.820 kb on + strand, within ABZR87_RS19585 at 766.820 kb on + strand, within ABZR87_RS19585 at 766.820 kb on + strand, within ABZR87_RS19585 at 766.821 kb on - strand, within ABZR87_RS19585 at 766.821 kb on - strand, within ABZR87_RS19585 at 766.821 kb on - strand, within ABZR87_RS19585 at 766.821 kb on - strand, within ABZR87_RS19585 at 766.821 kb on - strand, within ABZR87_RS19585 at 766.859 kb on + strand, within ABZR87_RS19585 at 766.859 kb on + strand, within ABZR87_RS19585 at 766.859 kb on + strand, within ABZR87_RS19585 at 766.859 kb on + strand, within ABZR87_RS19585 at 766.860 kb on - strand, within ABZR87_RS19585 at 766.860 kb on - strand, within ABZR87_RS19585 at 766.860 kb on - strand, within ABZR87_RS19585 at 766.860 kb on - strand, within ABZR87_RS19585 at 766.860 kb on - strand, within ABZR87_RS19585 at 766.904 kb on + strand, within ABZR87_RS19585 at 766.904 kb on + strand, within ABZR87_RS19585 at 766.905 kb on - strand, within ABZR87_RS19585 at 766.997 kb on + strand, within ABZR87_RS19585 at 767.052 kb on + strand, within ABZR87_RS19585 at 767.116 kb on - strand, within ABZR87_RS19585 at 767.162 kb on + strand, within ABZR87_RS19585 at 767.162 kb on + strand, within ABZR87_RS19585 at 767.162 kb on + strand, within ABZR87_RS19585 at 767.163 kb on - strand, within ABZR87_RS19585 at 767.163 kb on - strand, within ABZR87_RS19585 at 767.163 kb on - strand, within ABZR87_RS19585 at 767.163 kb on - strand, within ABZR87_RS19585 at 767.273 kb on + strand, within ABZR87_RS19585 at 767.279 kb on + strand, within ABZR87_RS19585 at 767.280 kb on - strand, within ABZR87_RS19585 at 767.456 kb on + strand, within ABZR87_RS19585 at 767.457 kb on - strand, within ABZR87_RS19585 at 767.457 kb on - strand, within ABZR87_RS19585 at 767.457 kb on - strand, within ABZR87_RS19585 at 767.457 kb on - strand, within ABZR87_RS19585 at 767.606 kb on + strand, within ABZR87_RS19585 at 767.607 kb on - strand, within ABZR87_RS19585 at 767.696 kb on + strand, within ABZR87_RS19585 at 767.696 kb on + strand, within ABZR87_RS19585 at 767.697 kb on - strand, within ABZR87_RS19585 at 767.697 kb on - strand, within ABZR87_RS19585 at 767.921 kb on + strand, within ABZR87_RS19585 at 767.921 kb on + strand, within ABZR87_RS19585 at 768.095 kb on + strand, within ABZR87_RS19585 at 768.095 kb on + strand, within ABZR87_RS19585 at 768.096 kb on - strand, within ABZR87_RS19585 at 768.096 kb on - strand, within ABZR87_RS19585 at 768.122 kb on + strand, within ABZR87_RS19585 at 768.307 kb on + strand, within ABZR87_RS19585 at 768.515 kb on + strand, within ABZR87_RS19585 at 768.515 kb on + strand, within ABZR87_RS19585 at 768.515 kb on + strand, within ABZR87_RS19585 at 768.515 kb on + strand, within ABZR87_RS19585 at 768.516 kb on - strand, within ABZR87_RS19585 at 768.516 kb on - strand, within ABZR87_RS19585 at 768.609 kb on - strand, within ABZR87_RS19585 at 768.629 kb on + strand, within ABZR87_RS19585 at 768.630 kb on - strand, within ABZR87_RS19585 at 768.630 kb on - strand, within ABZR87_RS19585 at 768.864 kb on - strand at 768.921 kb on - strand at 768.986 kb on - strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.019 kb on + strand at 769.020 kb on - strand at 769.020 kb on - strand at 769.020 kb on - strand at 769.020 kb on - strand at 769.020 kb on - strand at 769.020 kb on - strand at 769.020 kb on - strand at 769.020 kb on - strand at 769.020 kb on - strand at 769.020 kb on - strand at 769.020 kb on - strand at 769.408 kb on + strand, within ABZR87_RS19590 at 769.409 kb on - strand, within ABZR87_RS19590 at 769.409 kb on - strand, within ABZR87_RS19590 at 769.409 kb on - strand, within ABZR87_RS19590 at 769.420 kb on + strand, within ABZR87_RS19590 at 769.420 kb on + strand, within ABZR87_RS19590 at 769.423 kb on - strand, within ABZR87_RS19590 at 769.633 kb on + strand at 769.633 kb on + strand at 769.633 kb on + strand at 769.696 kb on + strand at 769.697 kb on - strand at 769.921 kb on + strand, within ABZR87_RS19595 at 769.921 kb on + strand, within ABZR87_RS19595 at 769.921 kb on + strand, within ABZR87_RS19595 at 769.921 kb on + strand, within ABZR87_RS19595 at 769.921 kb on + strand, within ABZR87_RS19595 at 769.921 kb on + strand, within ABZR87_RS19595 at 769.921 kb on + strand, within ABZR87_RS19595 at 769.922 kb on - strand, within ABZR87_RS19595 at 769.922 kb on - strand, within ABZR87_RS19595 at 769.996 kb on + strand, within ABZR87_RS19595 at 769.997 kb on - strand, within ABZR87_RS19595 at 770.090 kb on - strand, within ABZR87_RS19595
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 764,948 + +1.1 764,949 - +1.0 764,949 - +0.6 764,949 - -0.9 764,950 + +2.0 764,951 - -0.5 764,951 - -0.1 764,951 - -0.7 764,951 - +0.2 765,083 - ABZR87_RS19580 0.11 +0.4 765,279 + ABZR87_RS19580 0.36 -0.9 765,335 + ABZR87_RS19580 0.43 +0.5 765,335 + ABZR87_RS19580 0.43 -0.5 765,335 + ABZR87_RS19580 0.43 +0.6 765,335 + ABZR87_RS19580 0.43 -1.7 765,335 + ABZR87_RS19580 0.43 +0.2 765,336 - ABZR87_RS19580 0.43 -0.5 765,336 - ABZR87_RS19580 0.43 -0.7 765,336 - ABZR87_RS19580 0.43 -0.3 765,336 - ABZR87_RS19580 0.43 -0.8 765,336 - ABZR87_RS19580 0.43 +0.0 765,336 - ABZR87_RS19580 0.43 +0.2 765,336 - ABZR87_RS19580 0.43 +0.1 765,347 + ABZR87_RS19580 0.45 -1.8 765,347 + ABZR87_RS19580 0.45 -1.0 765,347 + ABZR87_RS19580 0.45 +0.0 765,386 + ABZR87_RS19580 0.50 -0.7 765,386 + ABZR87_RS19580 0.50 +0.2 765,386 + ABZR87_RS19580 0.50 -1.2 765,386 + ABZR87_RS19580 0.50 -1.2 765,387 - ABZR87_RS19580 0.50 -2.1 765,387 - ABZR87_RS19580 0.50 -0.5 765,387 - ABZR87_RS19580 0.50 -0.0 765,387 - ABZR87_RS19580 0.50 -0.7 765,387 - ABZR87_RS19580 0.50 +0.8 765,387 - ABZR87_RS19580 0.50 -0.1 765,387 - ABZR87_RS19580 0.50 -0.7 765,389 + ABZR87_RS19580 0.50 -0.2 765,389 + ABZR87_RS19580 0.50 -0.3 765,389 + ABZR87_RS19580 0.50 -0.7 765,389 + ABZR87_RS19580 0.50 -0.7 765,389 + ABZR87_RS19580 0.50 -1.3 765,389 + ABZR87_RS19580 0.50 -1.9 765,389 + ABZR87_RS19580 0.50 +0.2 765,389 + ABZR87_RS19580 0.50 -0.9 765,390 - ABZR87_RS19580 0.50 +0.2 765,390 - ABZR87_RS19580 0.50 +0.1 765,390 - ABZR87_RS19580 0.50 -0.9 765,390 - ABZR87_RS19580 0.50 +0.2 765,464 + ABZR87_RS19580 0.59 -0.4 765,464 + ABZR87_RS19580 0.59 -0.2 765,465 - ABZR87_RS19580 0.60 +0.4 765,465 - ABZR87_RS19580 0.60 -1.0 765,605 - ABZR87_RS19580 0.77 -0.5 765,843 - -0.2 765,897 + -0.6 765,897 + -0.4 765,897 + -3.2 765,897 + +0.6 765,897 + -0.2 765,897 + -1.6 765,898 - +0.1 765,898 - -0.5 765,898 - -1.0 766,000 + +0.3 766,001 - +0.1 766,001 - -0.6 766,001 - -0.3 766,097 + -0.4 766,136 + -2.2 766,136 + -0.0 766,137 - +0.9 766,322 + ABZR87_RS19585 0.12 +0.5 766,323 - ABZR87_RS19585 0.12 -0.4 766,477 + ABZR87_RS19585 0.17 -0.6 766,820 + ABZR87_RS19585 0.27 -0.0 766,820 + ABZR87_RS19585 0.27 -0.6 766,820 + ABZR87_RS19585 0.27 -0.0 766,821 - ABZR87_RS19585 0.27 -0.2 766,821 - ABZR87_RS19585 0.27 +0.1 766,821 - ABZR87_RS19585 0.27 -0.6 766,821 - ABZR87_RS19585 0.27 +1.0 766,821 - ABZR87_RS19585 0.27 +0.2 766,859 + ABZR87_RS19585 0.29 +1.0 766,859 + ABZR87_RS19585 0.29 -0.7 766,859 + ABZR87_RS19585 0.29 -1.1 766,859 + ABZR87_RS19585 0.29 -0.8 766,860 - ABZR87_RS19585 0.29 +0.8 766,860 - ABZR87_RS19585 0.29 -0.1 766,860 - ABZR87_RS19585 0.29 -0.4 766,860 - ABZR87_RS19585 0.29 +0.2 766,860 - ABZR87_RS19585 0.29 +0.9 766,904 + ABZR87_RS19585 0.30 +0.1 766,904 + ABZR87_RS19585 0.30 -1.1 766,905 - ABZR87_RS19585 0.30 +0.0 766,997 + ABZR87_RS19585 0.33 +0.3 767,052 + ABZR87_RS19585 0.34 -0.2 767,116 - ABZR87_RS19585 0.36 +0.6 767,162 + ABZR87_RS19585 0.38 +1.4 767,162 + ABZR87_RS19585 0.38 +0.2 767,162 + ABZR87_RS19585 0.38 +2.6 767,163 - ABZR87_RS19585 0.38 -0.4 767,163 - ABZR87_RS19585 0.38 -1.4 767,163 - ABZR87_RS19585 0.38 -0.1 767,163 - ABZR87_RS19585 0.38 +0.3 767,273 + ABZR87_RS19585 0.41 +0.6 767,279 + ABZR87_RS19585 0.41 -1.6 767,280 - ABZR87_RS19585 0.42 +0.2 767,456 + ABZR87_RS19585 0.47 -0.3 767,457 - ABZR87_RS19585 0.47 -0.7 767,457 - ABZR87_RS19585 0.47 -0.3 767,457 - ABZR87_RS19585 0.47 -0.3 767,457 - ABZR87_RS19585 0.47 -1.4 767,606 + ABZR87_RS19585 0.52 -1.0 767,607 - ABZR87_RS19585 0.52 -0.4 767,696 + ABZR87_RS19585 0.54 -2.0 767,696 + ABZR87_RS19585 0.54 +0.0 767,697 - ABZR87_RS19585 0.54 +0.8 767,697 - ABZR87_RS19585 0.54 +0.7 767,921 + ABZR87_RS19585 0.61 +1.0 767,921 + ABZR87_RS19585 0.61 +1.7 768,095 + ABZR87_RS19585 0.67 -0.2 768,095 + ABZR87_RS19585 0.67 -0.9 768,096 - ABZR87_RS19585 0.67 -0.4 768,096 - ABZR87_RS19585 0.67 -0.1 768,122 + ABZR87_RS19585 0.68 -3.1 768,307 + ABZR87_RS19585 0.73 -0.6 768,515 + ABZR87_RS19585 0.80 -0.1 768,515 + ABZR87_RS19585 0.80 +0.8 768,515 + ABZR87_RS19585 0.80 -0.5 768,515 + ABZR87_RS19585 0.80 -0.1 768,516 - ABZR87_RS19585 0.80 -0.3 768,516 - ABZR87_RS19585 0.80 +1.4 768,609 - ABZR87_RS19585 0.83 +2.0 768,629 + ABZR87_RS19585 0.83 +0.5 768,630 - ABZR87_RS19585 0.83 +0.9 768,630 - ABZR87_RS19585 0.83 +0.2 768,864 - +0.9 768,921 - -0.3 768,986 - +1.1 769,019 + -0.3 769,019 + +2.4 769,019 + +0.7 769,019 + -0.0 769,019 + -1.2 769,019 + +1.7 769,019 + +0.3 769,019 + +0.1 769,019 + -0.1 769,019 + +0.2 769,019 + -0.0 769,019 + +0.3 769,019 + -1.8 769,020 - -0.6 769,020 - -0.4 769,020 - -1.1 769,020 - +1.4 769,020 - +0.1 769,020 - -0.5 769,020 - -0.0 769,020 - -0.0 769,020 - -0.8 769,020 - +0.8 769,020 - +0.1 769,408 + ABZR87_RS19590 0.22 -0.6 769,409 - ABZR87_RS19590 0.22 -1.0 769,409 - ABZR87_RS19590 0.22 +0.3 769,409 - ABZR87_RS19590 0.22 -1.3 769,420 + ABZR87_RS19590 0.26 +0.4 769,420 + ABZR87_RS19590 0.26 +0.8 769,423 - ABZR87_RS19590 0.27 +0.1 769,633 + -1.1 769,633 + -0.5 769,633 + -0.4 769,696 + -1.2 769,697 - +0.1 769,921 + ABZR87_RS19595 0.20 -0.3 769,921 + ABZR87_RS19595 0.20 +0.0 769,921 + ABZR87_RS19595 0.20 -0.7 769,921 + ABZR87_RS19595 0.20 -1.0 769,921 + ABZR87_RS19595 0.20 -1.9 769,921 + ABZR87_RS19595 0.20 -0.4 769,921 + ABZR87_RS19595 0.20 +0.1 769,922 - ABZR87_RS19595 0.20 -1.6 769,922 - ABZR87_RS19595 0.20 -0.5 769,996 + ABZR87_RS19595 0.26 +0.0 769,997 - ABZR87_RS19595 0.26 +0.2 770,090 - ABZR87_RS19595 0.34 -0.4
Or see this region's nucleotide sequence