Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS19510 and ABZR87_RS19515 are separated by 566 nucleotides ABZR87_RS19515 and ABZR87_RS19520 are separated by 92 nucleotides ABZR87_RS19520 and ABZR87_RS19525 are separated by 62 nucleotides ABZR87_RS19525 and ABZR87_RS19530 are separated by 76 nucleotides
ABZR87_RS19510: ABZR87_RS19510 - collagen-like triple helix repeat-containing protein, at 750,481 to 751,605
_RS19510
ABZR87_RS19515: ABZR87_RS19515 - Flp family type IVb pilin, at 752,172 to 752,351
_RS19515
ABZR87_RS19520: ABZR87_RS19520 - prepilin peptidase, at 752,444 to 752,908
_RS19520
ABZR87_RS19525: ABZR87_RS19525 - TadE/TadG family type IV pilus assembly protein, at 752,971 to 753,474
_RS19525
ABZR87_RS19530: ABZR87_RS19530 - Flp pilus assembly protein CpaB, at 753,551 to 754,477
_RS19530
Position (kb) 752
753 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 751.567 kb on - strand at 751.567 kb on - strand at 751.610 kb on + strand at 751.610 kb on + strand at 751.610 kb on + strand at 751.610 kb on + strand at 751.610 kb on + strand at 751.610 kb on + strand at 751.610 kb on + strand at 751.610 kb on + strand at 751.611 kb on - strand at 751.611 kb on - strand at 751.714 kb on + strand at 751.734 kb on + strand at 751.734 kb on + strand at 751.734 kb on + strand at 751.735 kb on - strand at 751.758 kb on + strand at 751.768 kb on - strand at 751.772 kb on + strand at 751.811 kb on + strand at 751.854 kb on - strand at 751.878 kb on + strand at 751.878 kb on + strand at 751.879 kb on - strand at 751.899 kb on + strand at 751.908 kb on + strand at 751.908 kb on + strand at 751.909 kb on - strand at 751.909 kb on - strand at 751.909 kb on - strand at 751.909 kb on - strand at 751.909 kb on - strand at 751.909 kb on - strand at 751.909 kb on - strand at 751.909 kb on - strand at 751.977 kb on + strand at 751.978 kb on - strand at 752.008 kb on + strand at 752.057 kb on + strand at 752.057 kb on + strand at 752.057 kb on + strand at 752.074 kb on + strand at 752.075 kb on - strand at 752.082 kb on - strand at 752.084 kb on + strand at 752.085 kb on - strand at 752.092 kb on - strand at 752.092 kb on - strand at 752.229 kb on + strand, within ABZR87_RS19515 at 752.339 kb on + strand at 752.339 kb on + strand at 752.340 kb on - strand at 752.340 kb on - strand at 752.350 kb on - strand at 752.374 kb on + strand at 752.374 kb on + strand at 752.374 kb on + strand at 752.397 kb on + strand at 752.397 kb on + strand at 752.397 kb on + strand at 752.398 kb on - strand at 752.398 kb on - strand at 752.398 kb on - strand at 752.398 kb on - strand at 752.398 kb on - strand at 752.416 kb on + strand at 752.416 kb on + strand at 752.416 kb on + strand at 752.419 kb on + strand at 752.419 kb on + strand at 752.419 kb on + strand at 752.419 kb on + strand at 752.419 kb on + strand at 752.419 kb on + strand at 752.419 kb on + strand at 752.419 kb on + strand at 752.419 kb on + strand at 752.419 kb on + strand at 752.420 kb on - strand at 752.420 kb on - strand at 752.420 kb on - strand at 752.420 kb on - strand at 752.420 kb on - strand at 752.420 kb on - strand at 752.420 kb on - strand at 752.522 kb on + strand, within ABZR87_RS19520 at 752.523 kb on - strand, within ABZR87_RS19520 at 752.523 kb on - strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.582 kb on + strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.583 kb on - strand, within ABZR87_RS19520 at 752.687 kb on - strand, within ABZR87_RS19520 at 752.687 kb on - strand, within ABZR87_RS19520 at 752.687 kb on - strand, within ABZR87_RS19520 at 752.819 kb on - strand, within ABZR87_RS19520 at 752.819 kb on - strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.834 kb on + strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.835 kb on - strand, within ABZR87_RS19520 at 752.836 kb on + strand, within ABZR87_RS19520 at 752.836 kb on + strand, within ABZR87_RS19520 at 752.837 kb on - strand, within ABZR87_RS19520 at 752.907 kb on - strand at 752.907 kb on - strand at 752.907 kb on - strand at 752.907 kb on - strand at 752.953 kb on + strand at 752.953 kb on + strand at 752.954 kb on - strand at 752.955 kb on + strand at 752.956 kb on - strand at 752.956 kb on - strand at 752.956 kb on - strand at 752.956 kb on - strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.004 kb on + strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.005 kb on - strand at 753.019 kb on - strand at 753.032 kb on + strand, within ABZR87_RS19525 at 753.130 kb on + strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.131 kb on - strand, within ABZR87_RS19525 at 753.133 kb on + strand, within ABZR87_RS19525 at 753.134 kb on - strand, within ABZR87_RS19525 at 753.134 kb on - strand, within ABZR87_RS19525 at 753.134 kb on - strand, within ABZR87_RS19525 at 753.134 kb on - strand, within ABZR87_RS19525 at 753.206 kb on - strand, within ABZR87_RS19525 at 753.206 kb on - strand, within ABZR87_RS19525 at 753.206 kb on - strand, within ABZR87_RS19525 at 753.242 kb on - strand, within ABZR87_RS19525 at 753.242 kb on - strand, within ABZR87_RS19525 at 753.261 kb on - strand, within ABZR87_RS19525 at 753.407 kb on + strand, within ABZR87_RS19525 at 753.408 kb on - strand, within ABZR87_RS19525 at 753.408 kb on - strand, within ABZR87_RS19525 at 753.472 kb on + strand at 753.472 kb on + strand at 753.472 kb on + strand at 753.472 kb on + strand at 753.473 kb on - strand at 753.473 kb on - strand at 753.473 kb on - strand at 753.536 kb on - strand at 753.619 kb on - strand at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.681 kb on + strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.682 kb on - strand, within ABZR87_RS19530 at 753.683 kb on + strand, within ABZR87_RS19530 at 753.698 kb on - strand, within ABZR87_RS19530 at 753.698 kb on - strand, within ABZR87_RS19530
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 751,567 - -2.1 751,567 - -0.5 751,610 + +0.5 751,610 + -0.6 751,610 + -0.2 751,610 + -0.2 751,610 + -1.4 751,610 + -0.1 751,610 + -1.2 751,610 + -1.4 751,611 - -0.1 751,611 - -0.0 751,714 + +0.6 751,734 + +0.6 751,734 + +0.2 751,734 + +0.2 751,735 - -1.5 751,758 + -1.3 751,768 - -2.1 751,772 + +0.6 751,811 + +0.6 751,854 - -0.8 751,878 + -0.8 751,878 + -0.5 751,879 - -1.1 751,899 + -0.3 751,908 + +1.7 751,908 + -0.5 751,909 - -0.2 751,909 - -0.5 751,909 - -0.7 751,909 - -0.9 751,909 - -1.8 751,909 - +0.2 751,909 - +1.6 751,909 - -2.7 751,977 + -1.0 751,978 - +0.7 752,008 + +0.3 752,057 + +0.4 752,057 + -0.4 752,057 + -0.8 752,074 + -1.2 752,075 - -1.5 752,082 - -3.0 752,084 + -1.0 752,085 - +0.4 752,092 - -0.1 752,092 - +0.2 752,229 + ABZR87_RS19515 0.32 -1.6 752,339 + +1.0 752,339 + -0.4 752,340 - -1.0 752,340 - -0.6 752,350 - -0.7 752,374 + -0.4 752,374 + -1.2 752,374 + -0.5 752,397 + +0.6 752,397 + +1.0 752,397 + -0.2 752,398 - -0.8 752,398 - -0.7 752,398 - -1.5 752,398 - -0.3 752,398 - +0.0 752,416 + -1.3 752,416 + -2.5 752,416 + +1.9 752,419 + -3.2 752,419 + -0.2 752,419 + -0.4 752,419 + -0.9 752,419 + -0.9 752,419 + -0.1 752,419 + -0.4 752,419 + -2.0 752,419 + -1.0 752,419 + +0.8 752,420 - -0.0 752,420 - -0.5 752,420 - +0.3 752,420 - -0.9 752,420 - -1.3 752,420 - -0.2 752,420 - +1.0 752,522 + ABZR87_RS19520 0.17 -0.5 752,523 - ABZR87_RS19520 0.17 -0.8 752,523 - ABZR87_RS19520 0.17 -0.1 752,582 + ABZR87_RS19520 0.30 -0.1 752,582 + ABZR87_RS19520 0.30 +0.1 752,582 + ABZR87_RS19520 0.30 -2.0 752,582 + ABZR87_RS19520 0.30 -0.2 752,582 + ABZR87_RS19520 0.30 -2.2 752,582 + ABZR87_RS19520 0.30 -0.8 752,583 - ABZR87_RS19520 0.30 -0.2 752,583 - ABZR87_RS19520 0.30 -0.1 752,583 - ABZR87_RS19520 0.30 -1.5 752,583 - ABZR87_RS19520 0.30 -1.7 752,583 - ABZR87_RS19520 0.30 -1.8 752,583 - ABZR87_RS19520 0.30 -0.3 752,583 - ABZR87_RS19520 0.30 +1.2 752,687 - ABZR87_RS19520 0.52 -1.3 752,687 - ABZR87_RS19520 0.52 -1.3 752,687 - ABZR87_RS19520 0.52 +1.8 752,819 - ABZR87_RS19520 0.81 -0.9 752,819 - ABZR87_RS19520 0.81 -1.2 752,834 + ABZR87_RS19520 0.84 +0.4 752,834 + ABZR87_RS19520 0.84 -1.2 752,834 + ABZR87_RS19520 0.84 +0.1 752,834 + ABZR87_RS19520 0.84 -1.1 752,834 + ABZR87_RS19520 0.84 -0.3 752,834 + ABZR87_RS19520 0.84 -1.0 752,834 + ABZR87_RS19520 0.84 +0.2 752,834 + ABZR87_RS19520 0.84 -0.6 752,834 + ABZR87_RS19520 0.84 +0.4 752,835 - ABZR87_RS19520 0.84 -0.7 752,835 - ABZR87_RS19520 0.84 -1.3 752,835 - ABZR87_RS19520 0.84 +0.0 752,835 - ABZR87_RS19520 0.84 -0.8 752,835 - ABZR87_RS19520 0.84 -0.9 752,835 - ABZR87_RS19520 0.84 -0.3 752,835 - ABZR87_RS19520 0.84 -0.2 752,835 - ABZR87_RS19520 0.84 -2.8 752,835 - ABZR87_RS19520 0.84 -1.7 752,835 - ABZR87_RS19520 0.84 -0.9 752,835 - ABZR87_RS19520 0.84 -0.8 752,835 - ABZR87_RS19520 0.84 -2.8 752,835 - ABZR87_RS19520 0.84 -0.5 752,835 - ABZR87_RS19520 0.84 -1.0 752,836 + ABZR87_RS19520 0.84 -2.2 752,836 + ABZR87_RS19520 0.84 -1.3 752,837 - ABZR87_RS19520 0.85 -0.3 752,907 - -0.9 752,907 - -0.6 752,907 - -0.3 752,907 - +0.3 752,953 + +1.3 752,953 + +0.5 752,954 - +1.0 752,955 + -1.2 752,956 - -1.0 752,956 - -0.8 752,956 - -0.6 752,956 - -1.4 753,004 + -0.8 753,004 + -0.7 753,004 + -0.7 753,004 + +0.3 753,004 + +0.1 753,004 + -1.0 753,004 + -0.0 753,004 + -0.2 753,004 + -0.7 753,004 + +2.3 753,005 - -0.9 753,005 - -0.9 753,005 - +0.5 753,005 - +1.0 753,005 - -0.9 753,005 - -1.0 753,005 - -1.1 753,005 - -2.5 753,005 - -0.6 753,005 - -2.0 753,005 - -1.9 753,005 - -0.8 753,005 - -0.3 753,019 - -1.0 753,032 + ABZR87_RS19525 0.12 -0.5 753,130 + ABZR87_RS19525 0.32 +0.0 753,131 - ABZR87_RS19525 0.32 -0.6 753,131 - ABZR87_RS19525 0.32 -1.4 753,131 - ABZR87_RS19525 0.32 -0.8 753,131 - ABZR87_RS19525 0.32 -2.0 753,131 - ABZR87_RS19525 0.32 -1.6 753,131 - ABZR87_RS19525 0.32 -0.5 753,131 - ABZR87_RS19525 0.32 -0.2 753,133 + ABZR87_RS19525 0.32 -0.4 753,134 - ABZR87_RS19525 0.32 -1.1 753,134 - ABZR87_RS19525 0.32 -0.4 753,134 - ABZR87_RS19525 0.32 -1.3 753,134 - ABZR87_RS19525 0.32 +0.9 753,206 - ABZR87_RS19525 0.47 -0.4 753,206 - ABZR87_RS19525 0.47 +0.3 753,206 - ABZR87_RS19525 0.47 -0.5 753,242 - ABZR87_RS19525 0.54 -0.2 753,242 - ABZR87_RS19525 0.54 -0.0 753,261 - ABZR87_RS19525 0.58 -1.5 753,407 + ABZR87_RS19525 0.87 -0.0 753,408 - ABZR87_RS19525 0.87 -0.4 753,408 - ABZR87_RS19525 0.87 -1.1 753,472 + +0.1 753,472 + +0.2 753,472 + -0.5 753,472 + +0.5 753,473 - +0.1 753,473 - +0.5 753,473 - -0.8 753,536 - -1.3 753,619 - +2.2 753,681 + ABZR87_RS19530 0.14 -0.5 753,681 + ABZR87_RS19530 0.14 -0.7 753,681 + ABZR87_RS19530 0.14 -0.2 753,681 + ABZR87_RS19530 0.14 -1.8 753,681 + ABZR87_RS19530 0.14 -0.3 753,681 + ABZR87_RS19530 0.14 +0.4 753,681 + ABZR87_RS19530 0.14 -0.9 753,682 - ABZR87_RS19530 0.14 -0.5 753,682 - ABZR87_RS19530 0.14 -0.0 753,682 - ABZR87_RS19530 0.14 -1.9 753,682 - ABZR87_RS19530 0.14 +0.5 753,682 - ABZR87_RS19530 0.14 +2.5 753,682 - ABZR87_RS19530 0.14 -0.3 753,682 - ABZR87_RS19530 0.14 -0.3 753,683 + ABZR87_RS19530 0.14 -0.1 753,698 - ABZR87_RS19530 0.16 -3.0 753,698 - ABZR87_RS19530 0.16 -0.4
Or see this region's nucleotide sequence