Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS19180 and ABZR87_RS19185 overlap by 17 nucleotides ABZR87_RS19185 and ABZR87_RS19190 are separated by 105 nucleotides ABZR87_RS19190 and ABZR87_RS19195 are separated by 82 nucleotides ABZR87_RS19195 and ABZR87_RS19200 are separated by 162 nucleotides
ABZR87_RS19180: ABZR87_RS19180 - ATP-binding protein, at 667,107 to 668,651
_RS19180
ABZR87_RS19185: ABZR87_RS19185 - response regulator, at 668,635 to 669,294
_RS19185
ABZR87_RS19190: ABZR87_RS19190 - Spy/CpxP family protein refolding chaperone, at 669,400 to 669,975
_RS19190
ABZR87_RS19195: ABZR87_RS19195 - universal stress protein, at 670,058 to 670,498
_RS19195
ABZR87_RS19200: ABZR87_RS19200 - AraC family transcriptional regulator, at 670,661 to 671,578
_RS19200
Position (kb) 669
670 Strain fitness (log2 ratio) -1
0
1
2
3 at 668.687 kb on - strand at 668.687 kb on - strand at 668.687 kb on - strand at 668.705 kb on - strand, within ABZR87_RS19185 at 668.731 kb on - strand, within ABZR87_RS19185 at 668.731 kb on - strand, within ABZR87_RS19185 at 668.920 kb on + strand, within ABZR87_RS19185 at 668.921 kb on - strand, within ABZR87_RS19185 at 669.038 kb on - strand, within ABZR87_RS19185 at 669.038 kb on - strand, within ABZR87_RS19185 at 669.038 kb on - strand, within ABZR87_RS19185 at 669.157 kb on + strand, within ABZR87_RS19185 at 669.158 kb on - strand, within ABZR87_RS19185 at 669.346 kb on - strand at 669.350 kb on + strand at 669.350 kb on + strand at 669.351 kb on + strand at 669.351 kb on - strand at 669.351 kb on - strand at 669.356 kb on + strand at 669.357 kb on - strand at 669.357 kb on - strand at 669.357 kb on - strand at 669.357 kb on - strand at 669.384 kb on + strand at 669.385 kb on - strand at 669.676 kb on + strand, within ABZR87_RS19190 at 669.708 kb on + strand, within ABZR87_RS19190 at 669.708 kb on + strand, within ABZR87_RS19190 at 669.709 kb on - strand, within ABZR87_RS19190 at 669.709 kb on - strand, within ABZR87_RS19190 at 669.763 kb on + strand, within ABZR87_RS19190 at 669.764 kb on - strand, within ABZR87_RS19190 at 669.973 kb on + strand at 670.066 kb on - strand at 670.089 kb on + strand at 670.089 kb on + strand at 670.090 kb on - strand at 670.212 kb on + strand, within ABZR87_RS19195 at 670.212 kb on + strand, within ABZR87_RS19195 at 670.213 kb on - strand, within ABZR87_RS19195 at 670.213 kb on - strand, within ABZR87_RS19195 at 670.213 kb on - strand, within ABZR87_RS19195 at 670.323 kb on + strand, within ABZR87_RS19195 at 670.323 kb on + strand, within ABZR87_RS19195 at 670.323 kb on + strand, within ABZR87_RS19195 at 670.323 kb on + strand, within ABZR87_RS19195 at 670.323 kb on + strand, within ABZR87_RS19195 at 670.324 kb on - strand, within ABZR87_RS19195 at 670.324 kb on - strand, within ABZR87_RS19195 at 670.324 kb on - strand, within ABZR87_RS19195 at 670.324 kb on - strand, within ABZR87_RS19195 at 670.324 kb on - strand, within ABZR87_RS19195 at 670.324 kb on - strand, within ABZR87_RS19195 at 670.324 kb on - strand, within ABZR87_RS19195 at 670.359 kb on + strand, within ABZR87_RS19195 at 670.360 kb on - strand, within ABZR87_RS19195 at 670.360 kb on - strand, within ABZR87_RS19195 at 670.393 kb on - strand, within ABZR87_RS19195 at 670.530 kb on + strand at 670.530 kb on + strand at 670.530 kb on + strand at 670.531 kb on - strand at 670.593 kb on + strand at 670.593 kb on + strand at 670.593 kb on + strand at 670.604 kb on + strand at 670.605 kb on - strand at 670.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 668,687 - +0.6 668,687 - -1.2 668,687 - -0.4 668,705 - ABZR87_RS19185 0.11 +0.2 668,731 - ABZR87_RS19185 0.15 -0.4 668,731 - ABZR87_RS19185 0.15 -0.0 668,920 + ABZR87_RS19185 0.43 +0.4 668,921 - ABZR87_RS19185 0.43 +1.1 669,038 - ABZR87_RS19185 0.61 +0.4 669,038 - ABZR87_RS19185 0.61 -1.4 669,038 - ABZR87_RS19185 0.61 +1.1 669,157 + ABZR87_RS19185 0.79 +1.3 669,158 - ABZR87_RS19185 0.79 +0.4 669,346 - -1.2 669,350 + -0.4 669,350 + -0.1 669,351 + +0.9 669,351 - -0.6 669,351 - -0.9 669,356 + +0.3 669,357 - -1.7 669,357 - +2.2 669,357 - -0.5 669,357 - +0.2 669,384 + -0.5 669,385 - +0.1 669,676 + ABZR87_RS19190 0.48 +1.4 669,708 + ABZR87_RS19190 0.53 -0.8 669,708 + ABZR87_RS19190 0.53 -0.3 669,709 - ABZR87_RS19190 0.54 +0.8 669,709 - ABZR87_RS19190 0.54 -0.1 669,763 + ABZR87_RS19190 0.63 -0.9 669,764 - ABZR87_RS19190 0.63 -0.0 669,973 + +2.0 670,066 - -0.2 670,089 + -1.6 670,089 + -0.8 670,090 - +1.7 670,212 + ABZR87_RS19195 0.35 +0.6 670,212 + ABZR87_RS19195 0.35 +1.0 670,213 - ABZR87_RS19195 0.35 +3.6 670,213 - ABZR87_RS19195 0.35 +0.2 670,213 - ABZR87_RS19195 0.35 -0.7 670,323 + ABZR87_RS19195 0.60 -1.0 670,323 + ABZR87_RS19195 0.60 -0.3 670,323 + ABZR87_RS19195 0.60 +0.5 670,323 + ABZR87_RS19195 0.60 +0.7 670,323 + ABZR87_RS19195 0.60 -0.3 670,324 - ABZR87_RS19195 0.60 -0.2 670,324 - ABZR87_RS19195 0.60 +0.4 670,324 - ABZR87_RS19195 0.60 +0.3 670,324 - ABZR87_RS19195 0.60 -1.3 670,324 - ABZR87_RS19195 0.60 +0.5 670,324 - ABZR87_RS19195 0.60 +0.1 670,324 - ABZR87_RS19195 0.60 -0.3 670,359 + ABZR87_RS19195 0.68 +0.9 670,360 - ABZR87_RS19195 0.68 +0.1 670,360 - ABZR87_RS19195 0.68 -0.1 670,393 - ABZR87_RS19195 0.76 +0.5 670,530 + -0.5 670,530 + -1.2 670,530 + +0.2 670,531 - -0.4 670,593 + -0.7 670,593 + +0.1 670,593 + -1.3 670,604 + +0.1 670,605 - -0.0 670,605 - +0.2
Or see this region's nucleotide sequence