Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18765 and ABZR87_RS18770 are separated by 94 nucleotides ABZR87_RS18770 and ABZR87_RS18775 are separated by 16 nucleotides ABZR87_RS18775 and ABZR87_RS18780 are separated by 457 nucleotides ABZR87_RS18780 and ABZR87_RS18785 are separated by 144 nucleotides
ABZR87_RS18765: ABZR87_RS18765 - S53 family peptidase, at 562,917 to 564,281
_RS18765
ABZR87_RS18770: ABZR87_RS18770 - S10 family peptidase, at 564,376 to 566,109
_RS18770
ABZR87_RS18775: ABZR87_RS18775 - hypothetical protein, at 566,126 to 566,257
_RS18775
ABZR87_RS18780: ABZR87_RS18780 - hypothetical protein, at 566,715 to 566,861
_RS18780
ABZR87_RS18785: ABZR87_RS18785 - FMN-dependent NADH-azoreductase, at 567,006 to 567,632
_RS18785
Position (kb) 564
565
566
567 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 563.484 kb on + strand, within ABZR87_RS18765 at 563.511 kb on + strand, within ABZR87_RS18765 at 563.511 kb on + strand, within ABZR87_RS18765 at 563.512 kb on - strand, within ABZR87_RS18765 at 563.598 kb on + strand, within ABZR87_RS18765 at 563.598 kb on + strand, within ABZR87_RS18765 at 563.598 kb on + strand, within ABZR87_RS18765 at 563.599 kb on - strand, within ABZR87_RS18765 at 563.599 kb on - strand, within ABZR87_RS18765 at 563.757 kb on + strand, within ABZR87_RS18765 at 563.757 kb on + strand, within ABZR87_RS18765 at 563.757 kb on + strand, within ABZR87_RS18765 at 563.758 kb on - strand, within ABZR87_RS18765 at 563.758 kb on - strand, within ABZR87_RS18765 at 563.772 kb on - strand, within ABZR87_RS18765 at 564.145 kb on + strand at 564.226 kb on + strand at 564.227 kb on - strand at 564.227 kb on - strand at 564.387 kb on + strand at 564.387 kb on + strand at 564.387 kb on + strand at 564.388 kb on - strand at 564.388 kb on - strand at 564.388 kb on - strand at 564.388 kb on - strand at 564.388 kb on - strand at 564.388 kb on - strand at 564.513 kb on + strand at 564.514 kb on - strand at 564.514 kb on - strand at 564.661 kb on + strand, within ABZR87_RS18770 at 564.662 kb on - strand, within ABZR87_RS18770 at 564.793 kb on + strand, within ABZR87_RS18770 at 564.793 kb on + strand, within ABZR87_RS18770 at 564.794 kb on - strand, within ABZR87_RS18770 at 564.794 kb on - strand, within ABZR87_RS18770 at 564.794 kb on - strand, within ABZR87_RS18770 at 564.794 kb on - strand, within ABZR87_RS18770 at 564.795 kb on + strand, within ABZR87_RS18770 at 564.970 kb on + strand, within ABZR87_RS18770 at 564.970 kb on + strand, within ABZR87_RS18770 at 565.111 kb on + strand, within ABZR87_RS18770 at 565.112 kb on - strand, within ABZR87_RS18770 at 565.112 kb on - strand, within ABZR87_RS18770 at 565.112 kb on - strand, within ABZR87_RS18770 at 565.141 kb on + strand, within ABZR87_RS18770 at 565.141 kb on + strand, within ABZR87_RS18770 at 565.141 kb on + strand, within ABZR87_RS18770 at 565.141 kb on + strand, within ABZR87_RS18770 at 565.141 kb on + strand, within ABZR87_RS18770 at 565.142 kb on - strand, within ABZR87_RS18770 at 565.142 kb on - strand, within ABZR87_RS18770 at 565.142 kb on - strand, within ABZR87_RS18770 at 565.142 kb on - strand, within ABZR87_RS18770 at 565.142 kb on - strand, within ABZR87_RS18770 at 565.204 kb on + strand, within ABZR87_RS18770 at 565.337 kb on + strand, within ABZR87_RS18770 at 565.338 kb on - strand, within ABZR87_RS18770 at 565.355 kb on - strand, within ABZR87_RS18770 at 565.418 kb on - strand, within ABZR87_RS18770 at 565.540 kb on + strand, within ABZR87_RS18770 at 565.540 kb on + strand, within ABZR87_RS18770 at 565.541 kb on - strand, within ABZR87_RS18770 at 565.639 kb on + strand, within ABZR87_RS18770 at 565.660 kb on + strand, within ABZR87_RS18770 at 565.660 kb on + strand, within ABZR87_RS18770 at 565.661 kb on - strand, within ABZR87_RS18770 at 565.661 kb on - strand, within ABZR87_RS18770 at 565.661 kb on - strand, within ABZR87_RS18770 at 565.801 kb on + strand, within ABZR87_RS18770 at 565.801 kb on + strand, within ABZR87_RS18770 at 565.801 kb on + strand, within ABZR87_RS18770 at 565.801 kb on + strand, within ABZR87_RS18770 at 565.801 kb on + strand, within ABZR87_RS18770 at 565.802 kb on - strand, within ABZR87_RS18770 at 565.802 kb on - strand, within ABZR87_RS18770 at 565.802 kb on - strand, within ABZR87_RS18770 at 565.802 kb on - strand, within ABZR87_RS18770 at 565.871 kb on - strand, within ABZR87_RS18770 at 565.873 kb on + strand, within ABZR87_RS18770 at 565.873 kb on + strand, within ABZR87_RS18770 at 565.874 kb on - strand, within ABZR87_RS18770 at 565.894 kb on + strand, within ABZR87_RS18770 at 565.895 kb on - strand, within ABZR87_RS18770 at 565.969 kb on + strand at 565.969 kb on + strand at 565.970 kb on - strand at 565.970 kb on - strand at 565.970 kb on - strand at 565.970 kb on - strand at 565.990 kb on + strand at 565.990 kb on + strand at 565.990 kb on + strand at 565.990 kb on + strand at 565.990 kb on + strand at 565.990 kb on + strand at 565.991 kb on - strand at 566.041 kb on + strand at 566.041 kb on + strand at 566.041 kb on + strand at 566.041 kb on + strand at 566.041 kb on + strand at 566.041 kb on + strand at 566.042 kb on - strand at 566.042 kb on - strand at 566.139 kb on + strand at 566.142 kb on + strand, within ABZR87_RS18775 at 566.142 kb on + strand, within ABZR87_RS18775 at 566.142 kb on + strand, within ABZR87_RS18775 at 566.143 kb on - strand, within ABZR87_RS18775 at 566.151 kb on + strand, within ABZR87_RS18775 at 566.151 kb on + strand, within ABZR87_RS18775 at 566.151 kb on + strand, within ABZR87_RS18775 at 566.151 kb on + strand, within ABZR87_RS18775 at 566.152 kb on - strand, within ABZR87_RS18775 at 566.152 kb on - strand, within ABZR87_RS18775 at 566.152 kb on - strand, within ABZR87_RS18775 at 566.152 kb on - strand, within ABZR87_RS18775 at 566.152 kb on - strand, within ABZR87_RS18775 at 566.154 kb on + strand, within ABZR87_RS18775 at 566.154 kb on + strand, within ABZR87_RS18775 at 566.155 kb on - strand, within ABZR87_RS18775 at 566.155 kb on - strand, within ABZR87_RS18775 at 566.155 kb on - strand, within ABZR87_RS18775 at 566.169 kb on + strand, within ABZR87_RS18775 at 566.169 kb on + strand, within ABZR87_RS18775 at 566.169 kb on + strand, within ABZR87_RS18775 at 566.170 kb on - strand, within ABZR87_RS18775 at 566.170 kb on - strand, within ABZR87_RS18775 at 566.170 kb on - strand, within ABZR87_RS18775 at 566.173 kb on - strand, within ABZR87_RS18775 at 566.302 kb on + strand at 566.303 kb on - strand at 566.328 kb on + strand at 566.329 kb on - strand at 566.337 kb on + strand at 566.337 kb on + strand at 566.338 kb on - strand at 566.338 kb on - strand at 566.338 kb on - strand at 566.412 kb on + strand at 566.423 kb on + strand at 566.424 kb on - strand at 566.501 kb on + strand at 566.501 kb on + strand at 566.502 kb on - strand at 566.502 kb on - strand at 566.512 kb on + strand at 566.512 kb on + strand at 566.512 kb on + strand at 566.513 kb on - strand at 566.513 kb on - strand at 566.542 kb on + strand at 566.542 kb on + strand at 566.542 kb on + strand at 566.599 kb on + strand at 566.599 kb on + strand at 566.599 kb on + strand at 566.600 kb on - strand at 566.600 kb on - strand at 566.601 kb on + strand at 566.601 kb on + strand at 566.601 kb on + strand at 566.601 kb on + strand at 566.602 kb on - strand at 566.602 kb on - strand at 566.602 kb on - strand at 566.602 kb on - strand at 566.602 kb on - strand at 566.602 kb on - strand at 566.603 kb on + strand at 566.603 kb on + strand at 566.603 kb on + strand at 566.603 kb on + strand at 566.603 kb on + strand at 566.603 kb on + strand at 566.603 kb on + strand at 566.604 kb on - strand at 566.604 kb on - strand at 566.604 kb on - strand at 566.698 kb on + strand at 566.698 kb on + strand at 566.699 kb on - strand at 566.699 kb on - strand at 566.699 kb on - strand at 566.859 kb on + strand at 566.859 kb on + strand at 566.859 kb on + strand at 566.860 kb on - strand at 567.027 kb on + strand at 567.027 kb on + strand at 567.027 kb on + strand at 567.027 kb on + strand at 567.027 kb on + strand at 567.027 kb on + strand at 567.028 kb on - strand at 567.028 kb on - strand at 567.028 kb on - strand at 567.108 kb on + strand, within ABZR87_RS18785 at 567.108 kb on + strand, within ABZR87_RS18785 at 567.109 kb on - strand, within ABZR87_RS18785
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 563,484 + ABZR87_RS18765 0.42 -0.8 563,511 + ABZR87_RS18765 0.44 +0.2 563,511 + ABZR87_RS18765 0.44 -0.1 563,512 - ABZR87_RS18765 0.44 -0.7 563,598 + ABZR87_RS18765 0.50 -0.4 563,598 + ABZR87_RS18765 0.50 -0.2 563,598 + ABZR87_RS18765 0.50 +0.4 563,599 - ABZR87_RS18765 0.50 +0.0 563,599 - ABZR87_RS18765 0.50 +1.2 563,757 + ABZR87_RS18765 0.62 -0.6 563,757 + ABZR87_RS18765 0.62 +0.3 563,757 + ABZR87_RS18765 0.62 +0.8 563,758 - ABZR87_RS18765 0.62 -0.3 563,758 - ABZR87_RS18765 0.62 +0.3 563,772 - ABZR87_RS18765 0.63 +0.3 564,145 + +2.4 564,226 + +0.5 564,227 - +1.2 564,227 - -0.1 564,387 + +1.3 564,387 + -0.5 564,387 + +1.0 564,388 - +1.0 564,388 - -0.3 564,388 - -0.2 564,388 - -0.3 564,388 - -0.4 564,388 - +0.2 564,513 + +2.0 564,514 - -0.7 564,514 - +1.7 564,661 + ABZR87_RS18770 0.16 -0.9 564,662 - ABZR87_RS18770 0.16 +0.8 564,793 + ABZR87_RS18770 0.24 +0.4 564,793 + ABZR87_RS18770 0.24 -0.0 564,794 - ABZR87_RS18770 0.24 +0.2 564,794 - ABZR87_RS18770 0.24 -1.0 564,794 - ABZR87_RS18770 0.24 +0.4 564,794 - ABZR87_RS18770 0.24 -0.2 564,795 + ABZR87_RS18770 0.24 +0.8 564,970 + ABZR87_RS18770 0.34 +0.7 564,970 + ABZR87_RS18770 0.34 -0.1 565,111 + ABZR87_RS18770 0.42 -0.0 565,112 - ABZR87_RS18770 0.42 -1.6 565,112 - ABZR87_RS18770 0.42 -0.7 565,112 - ABZR87_RS18770 0.42 +0.3 565,141 + ABZR87_RS18770 0.44 -1.1 565,141 + ABZR87_RS18770 0.44 +0.0 565,141 + ABZR87_RS18770 0.44 -1.2 565,141 + ABZR87_RS18770 0.44 -2.0 565,141 + ABZR87_RS18770 0.44 -0.8 565,142 - ABZR87_RS18770 0.44 -1.4 565,142 - ABZR87_RS18770 0.44 +0.7 565,142 - ABZR87_RS18770 0.44 -1.1 565,142 - ABZR87_RS18770 0.44 -0.6 565,142 - ABZR87_RS18770 0.44 -0.5 565,204 + ABZR87_RS18770 0.48 -0.9 565,337 + ABZR87_RS18770 0.55 -0.3 565,338 - ABZR87_RS18770 0.55 +1.0 565,355 - ABZR87_RS18770 0.56 -1.2 565,418 - ABZR87_RS18770 0.60 -1.1 565,540 + ABZR87_RS18770 0.67 +0.6 565,540 + ABZR87_RS18770 0.67 -0.6 565,541 - ABZR87_RS18770 0.67 +0.3 565,639 + ABZR87_RS18770 0.73 +1.3 565,660 + ABZR87_RS18770 0.74 -0.2 565,660 + ABZR87_RS18770 0.74 +0.2 565,661 - ABZR87_RS18770 0.74 +1.0 565,661 - ABZR87_RS18770 0.74 -0.5 565,661 - ABZR87_RS18770 0.74 -0.4 565,801 + ABZR87_RS18770 0.82 +0.6 565,801 + ABZR87_RS18770 0.82 -0.8 565,801 + ABZR87_RS18770 0.82 -0.6 565,801 + ABZR87_RS18770 0.82 -0.5 565,801 + ABZR87_RS18770 0.82 -0.3 565,802 - ABZR87_RS18770 0.82 -1.8 565,802 - ABZR87_RS18770 0.82 -0.4 565,802 - ABZR87_RS18770 0.82 -0.9 565,802 - ABZR87_RS18770 0.82 +0.2 565,871 - ABZR87_RS18770 0.86 +0.1 565,873 + ABZR87_RS18770 0.86 -0.1 565,873 + ABZR87_RS18770 0.86 -0.7 565,874 - ABZR87_RS18770 0.86 +0.5 565,894 + ABZR87_RS18770 0.88 -0.5 565,895 - ABZR87_RS18770 0.88 -0.2 565,969 + +0.4 565,969 + +0.3 565,970 - -0.0 565,970 - -0.1 565,970 - -2.5 565,970 - -1.6 565,990 + -0.7 565,990 + +0.8 565,990 + -0.4 565,990 + -1.3 565,990 + -1.8 565,990 + -0.3 565,991 - -2.2 566,041 + +0.0 566,041 + +0.2 566,041 + +0.2 566,041 + -0.4 566,041 + +0.0 566,041 + +0.5 566,042 - -0.3 566,042 - +0.3 566,139 + +0.2 566,142 + ABZR87_RS18775 0.12 -0.9 566,142 + ABZR87_RS18775 0.12 +1.0 566,142 + ABZR87_RS18775 0.12 -0.4 566,143 - ABZR87_RS18775 0.13 +0.3 566,151 + ABZR87_RS18775 0.19 -0.1 566,151 + ABZR87_RS18775 0.19 -1.8 566,151 + ABZR87_RS18775 0.19 -1.4 566,151 + ABZR87_RS18775 0.19 +0.0 566,152 - ABZR87_RS18775 0.20 +1.2 566,152 - ABZR87_RS18775 0.20 -0.5 566,152 - ABZR87_RS18775 0.20 -2.1 566,152 - ABZR87_RS18775 0.20 +0.4 566,152 - ABZR87_RS18775 0.20 +0.2 566,154 + ABZR87_RS18775 0.21 +0.2 566,154 + ABZR87_RS18775 0.21 -0.9 566,155 - ABZR87_RS18775 0.22 -0.9 566,155 - ABZR87_RS18775 0.22 -0.5 566,155 - ABZR87_RS18775 0.22 -0.8 566,169 + ABZR87_RS18775 0.33 -2.2 566,169 + ABZR87_RS18775 0.33 -0.4 566,169 + ABZR87_RS18775 0.33 +0.1 566,170 - ABZR87_RS18775 0.33 -0.2 566,170 - ABZR87_RS18775 0.33 -0.0 566,170 - ABZR87_RS18775 0.33 -2.9 566,173 - ABZR87_RS18775 0.36 -0.5 566,302 + +0.1 566,303 - -1.8 566,328 + -0.8 566,329 - -0.3 566,337 + +0.5 566,337 + -0.8 566,338 - -0.2 566,338 - -0.1 566,338 - +0.1 566,412 + -0.9 566,423 + -1.1 566,424 - +1.3 566,501 + -2.1 566,501 + -2.0 566,502 - +0.6 566,502 - -0.4 566,512 + +0.3 566,512 + -0.0 566,512 + +0.4 566,513 - -1.4 566,513 - -0.6 566,542 + -0.8 566,542 + +0.6 566,542 + +1.3 566,599 + +0.4 566,599 + -0.2 566,599 + +0.1 566,600 - +0.4 566,600 - +0.6 566,601 + +0.6 566,601 + +1.0 566,601 + -0.7 566,601 + +0.1 566,602 - -0.2 566,602 - -1.7 566,602 - -0.7 566,602 - +1.2 566,602 - -1.2 566,602 - -2.6 566,603 + -0.1 566,603 + -1.6 566,603 + -0.1 566,603 + -1.3 566,603 + -2.1 566,603 + +0.6 566,603 + -0.8 566,604 - -0.3 566,604 - -1.8 566,604 - +1.1 566,698 + +0.7 566,698 + +0.6 566,699 - +0.1 566,699 - +0.5 566,699 - +0.2 566,859 + +0.8 566,859 + -1.3 566,859 + +1.4 566,860 - -1.4 567,027 + -0.4 567,027 + -0.3 567,027 + -0.5 567,027 + -0.9 567,027 + +1.0 567,027 + -2.7 567,028 - -1.0 567,028 - +0.3 567,028 - +0.4 567,108 + ABZR87_RS18785 0.16 -1.0 567,108 + ABZR87_RS18785 0.16 -0.7 567,109 - ABZR87_RS18785 0.16 +0.6
Or see this region's nucleotide sequence