Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18605 and ABZR87_RS18610 are separated by 88 nucleotides ABZR87_RS18610 and ABZR87_RS18615 overlap by 4 nucleotides ABZR87_RS18615 and ABZR87_RS18620 overlap by 4 nucleotides ABZR87_RS18620 and ABZR87_RS18625 are separated by 1 nucleotides
ABZR87_RS18605: ABZR87_RS18605 - SPFH domain-containing protein, at 527,499 to 528,542
_RS18605
ABZR87_RS18610: ABZR87_RS18610 - DUF4178 domain-containing protein, at 528,631 to 530,079
_RS18610
ABZR87_RS18615: ABZR87_RS18615 - hypothetical protein, at 530,076 to 530,264
_RS18615
ABZR87_RS18620: ABZR87_RS18620 - adenosylmethionine decarboxylase, at 530,261 to 530,686
_RS18620
ABZR87_RS18625: ABZR87_RS18625 - sensor domain-containing diguanylate cyclase, at 530,688 to 531,734
_RS18625
Position (kb)
528
529
530
531 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 527.680 kb on + strand, within ABZR87_RS18605 at 527.680 kb on + strand, within ABZR87_RS18605 at 527.681 kb on - strand, within ABZR87_RS18605 at 527.733 kb on + strand, within ABZR87_RS18605 at 527.733 kb on + strand, within ABZR87_RS18605 at 527.734 kb on - strand, within ABZR87_RS18605 at 527.784 kb on + strand, within ABZR87_RS18605 at 527.785 kb on - strand, within ABZR87_RS18605 at 527.785 kb on - strand, within ABZR87_RS18605 at 527.787 kb on + strand, within ABZR87_RS18605 at 527.788 kb on - strand, within ABZR87_RS18605 at 527.892 kb on + strand, within ABZR87_RS18605 at 527.892 kb on + strand, within ABZR87_RS18605 at 527.898 kb on + strand, within ABZR87_RS18605 at 527.898 kb on + strand, within ABZR87_RS18605 at 527.925 kb on + strand, within ABZR87_RS18605 at 527.926 kb on - strand, within ABZR87_RS18605 at 527.955 kb on + strand, within ABZR87_RS18605 at 527.955 kb on + strand, within ABZR87_RS18605 at 527.955 kb on + strand, within ABZR87_RS18605 at 527.956 kb on - strand, within ABZR87_RS18605 at 527.956 kb on - strand, within ABZR87_RS18605 at 527.956 kb on - strand, within ABZR87_RS18605 at 527.956 kb on - strand, within ABZR87_RS18605 at 527.956 kb on - strand, within ABZR87_RS18605 at 528.676 kb on - strand at 528.784 kb on + strand, within ABZR87_RS18610 at 528.784 kb on + strand, within ABZR87_RS18610 at 528.784 kb on + strand, within ABZR87_RS18610 at 528.785 kb on - strand, within ABZR87_RS18610 at 528.785 kb on - strand, within ABZR87_RS18610 at 528.785 kb on - strand, within ABZR87_RS18610 at 528.889 kb on + strand, within ABZR87_RS18610 at 528.889 kb on + strand, within ABZR87_RS18610 at 528.889 kb on + strand, within ABZR87_RS18610 at 528.889 kb on + strand, within ABZR87_RS18610 at 528.889 kb on + strand, within ABZR87_RS18610 at 528.890 kb on - strand, within ABZR87_RS18610 at 529.016 kb on - strand, within ABZR87_RS18610 at 529.016 kb on - strand, within ABZR87_RS18610 at 529.016 kb on - strand, within ABZR87_RS18610 at 529.036 kb on + strand, within ABZR87_RS18610 at 529.037 kb on - strand, within ABZR87_RS18610 at 529.037 kb on - strand, within ABZR87_RS18610 at 529.037 kb on - strand, within ABZR87_RS18610 at 529.037 kb on - strand, within ABZR87_RS18610 at 529.037 kb on - strand, within ABZR87_RS18610 at 529.166 kb on - strand, within ABZR87_RS18610 at 529.166 kb on - strand, within ABZR87_RS18610 at 529.192 kb on + strand, within ABZR87_RS18610 at 529.193 kb on - strand, within ABZR87_RS18610 at 529.549 kb on + strand, within ABZR87_RS18610 at 529.550 kb on - strand, within ABZR87_RS18610 at 529.550 kb on - strand, within ABZR87_RS18610 at 529.559 kb on - strand, within ABZR87_RS18610 at 529.682 kb on - strand, within ABZR87_RS18610 at 529.699 kb on + strand, within ABZR87_RS18610 at 529.700 kb on - strand, within ABZR87_RS18610 at 529.700 kb on - strand, within ABZR87_RS18610 at 529.717 kb on + strand, within ABZR87_RS18610 at 529.717 kb on + strand, within ABZR87_RS18610 at 529.717 kb on + strand, within ABZR87_RS18610 at 529.769 kb on + strand, within ABZR87_RS18610 at 529.921 kb on + strand, within ABZR87_RS18610 at 529.921 kb on + strand, within ABZR87_RS18610 at 529.921 kb on + strand, within ABZR87_RS18610 at 529.921 kb on + strand, within ABZR87_RS18610 at 529.921 kb on + strand, within ABZR87_RS18610 at 529.922 kb on - strand, within ABZR87_RS18610 at 529.922 kb on - strand, within ABZR87_RS18610 at 529.922 kb on - strand, within ABZR87_RS18610 at 530.007 kb on + strand at 530.097 kb on + strand, within ABZR87_RS18615 at 530.097 kb on + strand, within ABZR87_RS18615 at 530.098 kb on - strand, within ABZR87_RS18615 at 530.098 kb on - strand, within ABZR87_RS18615 at 530.127 kb on + strand, within ABZR87_RS18615 at 530.127 kb on + strand, within ABZR87_RS18615 at 530.127 kb on + strand, within ABZR87_RS18615 at 530.127 kb on + strand, within ABZR87_RS18615 at 530.127 kb on + strand, within ABZR87_RS18615 at 530.127 kb on + strand, within ABZR87_RS18615 at 530.127 kb on + strand, within ABZR87_RS18615 at 530.128 kb on - strand, within ABZR87_RS18615 at 530.128 kb on - strand, within ABZR87_RS18615 at 530.128 kb on - strand, within ABZR87_RS18615 at 530.128 kb on - strand, within ABZR87_RS18615 at 530.129 kb on + strand, within ABZR87_RS18615 at 530.129 kb on + strand, within ABZR87_RS18615 at 530.130 kb on - strand, within ABZR87_RS18615 at 530.130 kb on - strand, within ABZR87_RS18615 at 530.172 kb on + strand, within ABZR87_RS18615 at 530.184 kb on - strand, within ABZR87_RS18615 at 530.184 kb on - strand, within ABZR87_RS18615 at 530.202 kb on + strand, within ABZR87_RS18615 at 530.336 kb on + strand, within ABZR87_RS18620 at 530.337 kb on - strand, within ABZR87_RS18620 at 530.533 kb on + strand, within ABZR87_RS18620 at 530.534 kb on - strand, within ABZR87_RS18620 at 530.534 kb on - strand, within ABZR87_RS18620 at 530.689 kb on + strand at 530.689 kb on + strand at 530.689 kb on + strand at 530.689 kb on + strand at 530.689 kb on + strand at 530.690 kb on - strand at 530.690 kb on - strand at 530.709 kb on + strand at 530.710 kb on - strand at 530.710 kb on - strand at 530.924 kb on + strand, within ABZR87_RS18625 at 531.046 kb on + strand, within ABZR87_RS18625 at 531.046 kb on + strand, within ABZR87_RS18625 at 531.046 kb on + strand, within ABZR87_RS18625 at 531.046 kb on + strand, within ABZR87_RS18625 at 531.047 kb on - strand, within ABZR87_RS18625 at 531.050 kb on + strand, within ABZR87_RS18625 at 531.051 kb on - strand, within ABZR87_RS18625
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 527,680 + ABZR87_RS18605 0.17 -1.1 527,680 + ABZR87_RS18605 0.17 -0.1 527,681 - ABZR87_RS18605 0.17 -0.3 527,733 + ABZR87_RS18605 0.22 -0.7 527,733 + ABZR87_RS18605 0.22 +0.8 527,734 - ABZR87_RS18605 0.23 -0.2 527,784 + ABZR87_RS18605 0.27 -0.5 527,785 - ABZR87_RS18605 0.27 +0.6 527,785 - ABZR87_RS18605 0.27 +0.4 527,787 + ABZR87_RS18605 0.28 +0.3 527,788 - ABZR87_RS18605 0.28 -0.6 527,892 + ABZR87_RS18605 0.38 +0.4 527,892 + ABZR87_RS18605 0.38 +0.4 527,898 + ABZR87_RS18605 0.38 +0.4 527,898 + ABZR87_RS18605 0.38 -0.1 527,925 + ABZR87_RS18605 0.41 -1.0 527,926 - ABZR87_RS18605 0.41 -0.0 527,955 + ABZR87_RS18605 0.44 -0.3 527,955 + ABZR87_RS18605 0.44 -0.3 527,955 + ABZR87_RS18605 0.44 +0.8 527,956 - ABZR87_RS18605 0.44 -0.3 527,956 - ABZR87_RS18605 0.44 +0.3 527,956 - ABZR87_RS18605 0.44 +0.3 527,956 - ABZR87_RS18605 0.44 +0.0 527,956 - ABZR87_RS18605 0.44 -0.1 528,676 - +0.9 528,784 + ABZR87_RS18610 0.11 -0.7 528,784 + ABZR87_RS18610 0.11 -0.9 528,784 + ABZR87_RS18610 0.11 -0.1 528,785 - ABZR87_RS18610 0.11 -0.8 528,785 - ABZR87_RS18610 0.11 -2.3 528,785 - ABZR87_RS18610 0.11 +0.8 528,889 + ABZR87_RS18610 0.18 -2.4 528,889 + ABZR87_RS18610 0.18 -0.0 528,889 + ABZR87_RS18610 0.18 -0.2 528,889 + ABZR87_RS18610 0.18 +0.0 528,889 + ABZR87_RS18610 0.18 +0.7 528,890 - ABZR87_RS18610 0.18 -1.0 529,016 - ABZR87_RS18610 0.27 -0.4 529,016 - ABZR87_RS18610 0.27 +0.5 529,016 - ABZR87_RS18610 0.27 +0.3 529,036 + ABZR87_RS18610 0.28 +0.0 529,037 - ABZR87_RS18610 0.28 -0.0 529,037 - ABZR87_RS18610 0.28 -0.2 529,037 - ABZR87_RS18610 0.28 +0.6 529,037 - ABZR87_RS18610 0.28 -2.0 529,037 - ABZR87_RS18610 0.28 -1.2 529,166 - ABZR87_RS18610 0.37 +0.2 529,166 - ABZR87_RS18610 0.37 -0.5 529,192 + ABZR87_RS18610 0.39 +0.7 529,193 - ABZR87_RS18610 0.39 +0.4 529,549 + ABZR87_RS18610 0.63 -0.3 529,550 - ABZR87_RS18610 0.63 +1.1 529,550 - ABZR87_RS18610 0.63 +1.0 529,559 - ABZR87_RS18610 0.64 -0.0 529,682 - ABZR87_RS18610 0.73 -0.2 529,699 + ABZR87_RS18610 0.74 -0.3 529,700 - ABZR87_RS18610 0.74 -0.4 529,700 - ABZR87_RS18610 0.74 +1.0 529,717 + ABZR87_RS18610 0.75 +1.4 529,717 + ABZR87_RS18610 0.75 +0.8 529,717 + ABZR87_RS18610 0.75 -1.7 529,769 + ABZR87_RS18610 0.79 +0.0 529,921 + ABZR87_RS18610 0.89 +0.6 529,921 + ABZR87_RS18610 0.89 -1.8 529,921 + ABZR87_RS18610 0.89 +0.3 529,921 + ABZR87_RS18610 0.89 +0.1 529,921 + ABZR87_RS18610 0.89 -1.8 529,922 - ABZR87_RS18610 0.89 +2.2 529,922 - ABZR87_RS18610 0.89 -0.0 529,922 - ABZR87_RS18610 0.89 +0.3 530,007 + +0.2 530,097 + ABZR87_RS18615 0.11 -0.1 530,097 + ABZR87_RS18615 0.11 +0.4 530,098 - ABZR87_RS18615 0.12 -1.0 530,098 - ABZR87_RS18615 0.12 -0.5 530,127 + ABZR87_RS18615 0.27 -0.8 530,127 + ABZR87_RS18615 0.27 -0.4 530,127 + ABZR87_RS18615 0.27 +0.8 530,127 + ABZR87_RS18615 0.27 -0.2 530,127 + ABZR87_RS18615 0.27 -1.1 530,127 + ABZR87_RS18615 0.27 +0.4 530,127 + ABZR87_RS18615 0.27 +0.1 530,128 - ABZR87_RS18615 0.28 +0.4 530,128 - ABZR87_RS18615 0.28 +0.3 530,128 - ABZR87_RS18615 0.28 -0.2 530,128 - ABZR87_RS18615 0.28 -1.4 530,129 + ABZR87_RS18615 0.28 -0.7 530,129 + ABZR87_RS18615 0.28 -0.8 530,130 - ABZR87_RS18615 0.29 -1.1 530,130 - ABZR87_RS18615 0.29 -0.8 530,172 + ABZR87_RS18615 0.51 +0.3 530,184 - ABZR87_RS18615 0.57 -0.1 530,184 - ABZR87_RS18615 0.57 +0.2 530,202 + ABZR87_RS18615 0.67 +0.2 530,336 + ABZR87_RS18620 0.18 -0.3 530,337 - ABZR87_RS18620 0.18 -0.4 530,533 + ABZR87_RS18620 0.64 -0.1 530,534 - ABZR87_RS18620 0.64 -1.5 530,534 - ABZR87_RS18620 0.64 -2.7 530,689 + -0.5 530,689 + +0.2 530,689 + -0.6 530,689 + +0.6 530,689 + +0.1 530,690 - +0.8 530,690 - -0.8 530,709 + -0.8 530,710 - +1.0 530,710 - -0.8 530,924 + ABZR87_RS18625 0.23 -1.0 531,046 + ABZR87_RS18625 0.34 +0.1 531,046 + ABZR87_RS18625 0.34 -2.4 531,046 + ABZR87_RS18625 0.34 -1.4 531,046 + ABZR87_RS18625 0.34 +0.7 531,047 - ABZR87_RS18625 0.34 +0.4 531,050 + ABZR87_RS18625 0.35 +0.0 531,051 - ABZR87_RS18625 0.35 -0.3
Or see this region's nucleotide sequence