Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18140 and ABZR87_RS18145 are separated by 24 nucleotides ABZR87_RS18145 and ABZR87_RS18150 are separated by 110 nucleotides ABZR87_RS18150 and ABZR87_RS18155 are separated by 8 nucleotides
ABZR87_RS18140: ABZR87_RS18140 - type III secretion system export apparatus subunit SctR, at 433,673 to 434,332
_RS18140
ABZR87_RS18145: ABZR87_RS18145 - lytic transglycosylase domain-containing protein, at 434,357 to 435,109
_RS18145
ABZR87_RS18150: ABZR87_RS18150 - LuxR family transcriptional regulator, at 435,220 to 436,107
_RS18150
ABZR87_RS18155: ABZR87_RS18155 - LysR family transcriptional regulator, at 436,116 to 437,231
_RS18155
Position (kb) 435
436
437 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 434.325 kb on - strand at 434.630 kb on + strand, within ABZR87_RS18145 at 434.631 kb on - strand, within ABZR87_RS18145 at 434.820 kb on - strand, within ABZR87_RS18145 at 434.858 kb on + strand, within ABZR87_RS18145 at 435.168 kb on + strand at 435.168 kb on + strand at 435.169 kb on + strand at 435.185 kb on - strand at 435.185 kb on - strand at 435.238 kb on - strand at 435.425 kb on - strand, within ABZR87_RS18150 at 435.484 kb on + strand, within ABZR87_RS18150 at 435.541 kb on + strand, within ABZR87_RS18150 at 435.542 kb on - strand, within ABZR87_RS18150 at 435.568 kb on + strand, within ABZR87_RS18150 at 435.568 kb on + strand, within ABZR87_RS18150 at 435.583 kb on + strand, within ABZR87_RS18150 at 435.583 kb on + strand, within ABZR87_RS18150 at 435.584 kb on - strand, within ABZR87_RS18150 at 435.748 kb on + strand, within ABZR87_RS18150 at 435.748 kb on + strand, within ABZR87_RS18150 at 435.749 kb on - strand, within ABZR87_RS18150 at 435.749 kb on - strand, within ABZR87_RS18150 at 435.749 kb on - strand, within ABZR87_RS18150 at 435.878 kb on - strand, within ABZR87_RS18150 at 435.889 kb on + strand, within ABZR87_RS18150 at 436.031 kb on - strand at 436.100 kb on - strand at 436.106 kb on - strand at 436.192 kb on - strand at 436.192 kb on - strand at 436.299 kb on - strand, within ABZR87_RS18155 at 436.361 kb on - strand, within ABZR87_RS18155 at 436.433 kb on + strand, within ABZR87_RS18155 at 436.433 kb on + strand, within ABZR87_RS18155 at 436.434 kb on - strand, within ABZR87_RS18155 at 436.895 kb on + strand, within ABZR87_RS18155 at 436.895 kb on + strand, within ABZR87_RS18155 at 436.895 kb on + strand, within ABZR87_RS18155 at 436.895 kb on + strand, within ABZR87_RS18155 at 436.896 kb on - strand, within ABZR87_RS18155 at 436.896 kb on - strand, within ABZR87_RS18155 at 436.897 kb on + strand, within ABZR87_RS18155 at 436.897 kb on + strand, within ABZR87_RS18155 at 436.898 kb on - strand, within ABZR87_RS18155
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 434,325 - +0.1 434,630 + ABZR87_RS18145 0.36 +0.4 434,631 - ABZR87_RS18145 0.36 +0.7 434,820 - ABZR87_RS18145 0.61 -0.7 434,858 + ABZR87_RS18145 0.67 -0.4 435,168 + -0.5 435,168 + -0.2 435,169 + +1.1 435,185 - +1.6 435,185 - +0.6 435,238 - -0.0 435,425 - ABZR87_RS18150 0.23 +0.4 435,484 + ABZR87_RS18150 0.30 -0.2 435,541 + ABZR87_RS18150 0.36 -1.5 435,542 - ABZR87_RS18150 0.36 +0.3 435,568 + ABZR87_RS18150 0.39 +0.9 435,568 + ABZR87_RS18150 0.39 +1.0 435,583 + ABZR87_RS18150 0.41 -0.2 435,583 + ABZR87_RS18150 0.41 -0.3 435,584 - ABZR87_RS18150 0.41 -1.7 435,748 + ABZR87_RS18150 0.59 -0.8 435,748 + ABZR87_RS18150 0.59 -1.0 435,749 - ABZR87_RS18150 0.60 -1.7 435,749 - ABZR87_RS18150 0.60 -3.2 435,749 - ABZR87_RS18150 0.60 -0.0 435,878 - ABZR87_RS18150 0.74 +0.0 435,889 + ABZR87_RS18150 0.75 +0.8 436,031 - -0.0 436,100 - -0.0 436,106 - -0.0 436,192 - +0.1 436,192 - -0.5 436,299 - ABZR87_RS18155 0.16 -0.6 436,361 - ABZR87_RS18155 0.22 +0.4 436,433 + ABZR87_RS18155 0.28 -0.1 436,433 + ABZR87_RS18155 0.28 -1.3 436,434 - ABZR87_RS18155 0.28 +0.5 436,895 + ABZR87_RS18155 0.70 +0.6 436,895 + ABZR87_RS18155 0.70 -0.1 436,895 + ABZR87_RS18155 0.70 -0.5 436,895 + ABZR87_RS18155 0.70 +0.4 436,896 - ABZR87_RS18155 0.70 +1.4 436,896 - ABZR87_RS18155 0.70 +0.1 436,897 + ABZR87_RS18155 0.70 +0.8 436,897 + ABZR87_RS18155 0.70 -0.8 436,898 - ABZR87_RS18155 0.70 -0.0
Or see this region's nucleotide sequence