Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18135 and ABZR87_RS18140 overlap by 8 nucleotides ABZR87_RS18140 and ABZR87_RS18145 are separated by 24 nucleotides ABZR87_RS18145 and ABZR87_RS18150 are separated by 110 nucleotides
ABZR87_RS18135: ABZR87_RS18135 - FliM/FliN family flagellar motor switch protein, at 432,517 to 433,680
_RS18135
ABZR87_RS18140: ABZR87_RS18140 - type III secretion system export apparatus subunit SctR, at 433,673 to 434,332
_RS18140
ABZR87_RS18145: ABZR87_RS18145 - lytic transglycosylase domain-containing protein, at 434,357 to 435,109
_RS18145
ABZR87_RS18150: ABZR87_RS18150 - LuxR family transcriptional regulator, at 435,220 to 436,107
_RS18150
Position (kb) 433
434
435 Strain fitness (log2 ratio) -1
0
1 at 433.679 kb on - strand at 433.760 kb on + strand, within ABZR87_RS18140 at 433.760 kb on + strand, within ABZR87_RS18140 at 434.097 kb on + strand, within ABZR87_RS18140 at 434.097 kb on + strand, within ABZR87_RS18140 at 434.120 kb on - strand, within ABZR87_RS18140 at 434.325 kb on - strand at 434.630 kb on + strand, within ABZR87_RS18145 at 434.631 kb on - strand, within ABZR87_RS18145 at 434.820 kb on - strand, within ABZR87_RS18145 at 434.858 kb on + strand, within ABZR87_RS18145 at 435.168 kb on + strand at 435.168 kb on + strand at 435.169 kb on + strand at 435.185 kb on - strand at 435.185 kb on - strand at 435.238 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 433,679 - +0.2 433,760 + ABZR87_RS18140 0.13 -0.2 433,760 + ABZR87_RS18140 0.13 -1.2 434,097 + ABZR87_RS18140 0.64 -0.3 434,097 + ABZR87_RS18140 0.64 -0.2 434,120 - ABZR87_RS18140 0.68 -1.3 434,325 - +0.1 434,630 + ABZR87_RS18145 0.36 +0.4 434,631 - ABZR87_RS18145 0.36 +0.7 434,820 - ABZR87_RS18145 0.61 -0.7 434,858 + ABZR87_RS18145 0.67 -0.4 435,168 + -0.5 435,168 + -0.2 435,169 + +1.1 435,185 - +1.6 435,185 - +0.6 435,238 - -0.0
Or see this region's nucleotide sequence