Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18120 and ABZR87_RS18125 are separated by 38 nucleotides ABZR87_RS18125 and ABZR87_RS18130 overlap by 1 nucleotides ABZR87_RS18130 and ABZR87_RS18135 are separated by 296 nucleotides
ABZR87_RS18120: ABZR87_RS18120 - type III secretion system outer membrane ring subunit SctC, at 427,831 to 429,708
_RS18120
ABZR87_RS18125: ABZR87_RS18125 - flagellar biosynthetic protein FliQ, at 429,747 to 430,025
_RS18125
ABZR87_RS18130: ABZR87_RS18130 - type III secretion system export apparatus subunit SctV, at 430,025 to 432,220
_RS18130
ABZR87_RS18135: ABZR87_RS18135 - FliM/FliN family flagellar motor switch protein, at 432,517 to 433,680
_RS18135
Position (kb) 430
431
432
433 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 429.068 kb on + strand, within ABZR87_RS18120 at 429.152 kb on + strand, within ABZR87_RS18120 at 429.152 kb on + strand, within ABZR87_RS18120 at 429.152 kb on + strand, within ABZR87_RS18120 at 429.153 kb on - strand, within ABZR87_RS18120 at 429.153 kb on - strand, within ABZR87_RS18120 at 429.263 kb on + strand, within ABZR87_RS18120 at 429.263 kb on + strand, within ABZR87_RS18120 at 429.264 kb on - strand, within ABZR87_RS18120 at 429.264 kb on - strand, within ABZR87_RS18120 at 429.264 kb on - strand, within ABZR87_RS18120 at 429.408 kb on - strand, within ABZR87_RS18120 at 429.408 kb on - strand, within ABZR87_RS18120 at 429.425 kb on + strand, within ABZR87_RS18120 at 429.425 kb on + strand, within ABZR87_RS18120 at 429.425 kb on + strand, within ABZR87_RS18120 at 429.426 kb on - strand, within ABZR87_RS18120 at 429.428 kb on + strand, within ABZR87_RS18120 at 429.443 kb on + strand, within ABZR87_RS18120 at 429.661 kb on - strand at 430.185 kb on + strand at 430.185 kb on + strand at 430.185 kb on + strand at 430.186 kb on - strand at 430.929 kb on + strand, within ABZR87_RS18130 at 430.930 kb on - strand, within ABZR87_RS18130 at 430.930 kb on - strand, within ABZR87_RS18130 at 430.930 kb on - strand, within ABZR87_RS18130 at 430.930 kb on - strand, within ABZR87_RS18130 at 430.930 kb on - strand, within ABZR87_RS18130 at 430.930 kb on - strand, within ABZR87_RS18130 at 430.938 kb on + strand, within ABZR87_RS18130 at 430.939 kb on - strand, within ABZR87_RS18130 at 430.965 kb on + strand, within ABZR87_RS18130 at 430.965 kb on + strand, within ABZR87_RS18130 at 430.966 kb on - strand, within ABZR87_RS18130 at 431.869 kb on + strand, within ABZR87_RS18130 at 431.950 kb on + strand, within ABZR87_RS18130 at 432.000 kb on + strand, within ABZR87_RS18130 at 432.001 kb on - strand at 432.229 kb on + strand at 432.230 kb on - strand at 432.239 kb on + strand at 432.239 kb on + strand at 432.240 kb on - strand at 432.240 kb on - strand at 432.240 kb on - strand at 432.338 kb on + strand at 432.338 kb on + strand at 432.339 kb on - strand at 432.339 kb on - strand at 432.443 kb on + strand at 432.444 kb on - strand at 432.595 kb on + strand at 432.595 kb on + strand at 432.596 kb on - strand at 432.596 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 429,068 + ABZR87_RS18120 0.66 +0.4 429,152 + ABZR87_RS18120 0.70 +1.0 429,152 + ABZR87_RS18120 0.70 -0.3 429,152 + ABZR87_RS18120 0.70 -0.0 429,153 - ABZR87_RS18120 0.70 -1.2 429,153 - ABZR87_RS18120 0.70 +0.5 429,263 + ABZR87_RS18120 0.76 -0.2 429,263 + ABZR87_RS18120 0.76 -0.1 429,264 - ABZR87_RS18120 0.76 -0.9 429,264 - ABZR87_RS18120 0.76 -2.0 429,264 - ABZR87_RS18120 0.76 -0.3 429,408 - ABZR87_RS18120 0.84 +0.5 429,408 - ABZR87_RS18120 0.84 +0.8 429,425 + ABZR87_RS18120 0.85 +2.1 429,425 + ABZR87_RS18120 0.85 +0.5 429,425 + ABZR87_RS18120 0.85 -0.4 429,426 - ABZR87_RS18120 0.85 +0.3 429,428 + ABZR87_RS18120 0.85 -2.5 429,443 + ABZR87_RS18120 0.86 -0.5 429,661 - -0.4 430,185 + -1.5 430,185 + -1.2 430,185 + +0.3 430,186 - -3.5 430,929 + ABZR87_RS18130 0.41 +0.1 430,930 - ABZR87_RS18130 0.41 +0.0 430,930 - ABZR87_RS18130 0.41 +0.0 430,930 - ABZR87_RS18130 0.41 +0.1 430,930 - ABZR87_RS18130 0.41 +0.5 430,930 - ABZR87_RS18130 0.41 +0.3 430,930 - ABZR87_RS18130 0.41 -0.5 430,938 + ABZR87_RS18130 0.42 +0.8 430,939 - ABZR87_RS18130 0.42 +1.1 430,965 + ABZR87_RS18130 0.43 +0.8 430,965 + ABZR87_RS18130 0.43 -0.0 430,966 - ABZR87_RS18130 0.43 +0.2 431,869 + ABZR87_RS18130 0.84 -0.2 431,950 + ABZR87_RS18130 0.88 +1.0 432,000 + ABZR87_RS18130 0.90 +0.4 432,001 - -1.1 432,229 + +0.7 432,230 - +0.5 432,239 + -0.0 432,239 + -0.7 432,240 - -1.8 432,240 - +0.1 432,240 - +0.1 432,338 + +0.4 432,338 + +0.2 432,339 - -1.2 432,339 - -1.9 432,443 + -0.6 432,444 - -1.2 432,595 + +0.4 432,595 + +0.9 432,596 - -0.5 432,596 - +1.0
Or see this region's nucleotide sequence