Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18090 and ABZR87_RS18095 overlap by 19 nucleotides ABZR87_RS18095 and ABZR87_RS18100 are separated by 8 nucleotides ABZR87_RS18100 and ABZR87_RS18105 overlap by 4 nucleotides ABZR87_RS18105 and ABZR87_RS18110 are separated by 103 nucleotides ABZR87_RS18110 and ABZR87_RS18115 are separated by 116 nucleotides
ABZR87_RS18090: ABZR87_RS18090 - type III secretion system stator protein SctL, at 423,630 to 424,244
_RS18090
ABZR87_RS18095: ABZR87_RS18095 - hypothetical protein, at 424,226 to 424,900
_RS18095
ABZR87_RS18100: ABZR87_RS18100 - type III secretion system inner membrane ring lipoprotein SctJ, at 424,909 to 425,730
_RS18100
ABZR87_RS18105: ABZR87_RS18105 - hypothetical protein, at 425,727 to 426,152
_RS18105
ABZR87_RS18110: ABZR87_RS18110 - hypothetical protein, at 426,256 to 426,468
_RS18110
ABZR87_RS18115: ABZR87_RS18115 - FHA domain-containing protein, at 426,585 to 427,856
_RS18115
Position (kb) 424
425
426 Strain fitness (log2 ratio) -2
-1
0
1
2 at 424.012 kb on + strand, within ABZR87_RS18090 at 424.012 kb on + strand, within ABZR87_RS18090 at 424.013 kb on - strand, within ABZR87_RS18090 at 424.013 kb on - strand, within ABZR87_RS18090 at 424.013 kb on - strand, within ABZR87_RS18090 at 424.085 kb on - strand, within ABZR87_RS18090 at 424.168 kb on + strand, within ABZR87_RS18090 at 424.464 kb on + strand, within ABZR87_RS18095 at 424.631 kb on + strand, within ABZR87_RS18095 at 424.632 kb on - strand, within ABZR87_RS18095 at 424.632 kb on - strand, within ABZR87_RS18095 at 424.632 kb on - strand, within ABZR87_RS18095 at 424.632 kb on - strand, within ABZR87_RS18095 at 425.026 kb on + strand, within ABZR87_RS18100 at 425.026 kb on + strand, within ABZR87_RS18100 at 425.090 kb on + strand, within ABZR87_RS18100 at 425.169 kb on - strand, within ABZR87_RS18100 at 425.255 kb on + strand, within ABZR87_RS18100 at 425.311 kb on + strand, within ABZR87_RS18100 at 425.384 kb on + strand, within ABZR87_RS18100 at 425.384 kb on + strand, within ABZR87_RS18100 at 425.385 kb on - strand, within ABZR87_RS18100 at 425.391 kb on - strand, within ABZR87_RS18100 at 425.391 kb on - strand, within ABZR87_RS18100 at 425.840 kb on + strand, within ABZR87_RS18105 at 425.980 kb on - strand, within ABZR87_RS18105 at 426.236 kb on + strand at 426.236 kb on + strand at 426.237 kb on - strand at 426.254 kb on + strand at 426.254 kb on + strand at 426.254 kb on + strand at 426.254 kb on + strand at 426.254 kb on + strand at 426.254 kb on + strand at 426.255 kb on - strand at 426.255 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 424,012 + ABZR87_RS18090 0.62 -1.3 424,012 + ABZR87_RS18090 0.62 +0.8 424,013 - ABZR87_RS18090 0.62 +0.3 424,013 - ABZR87_RS18090 0.62 -0.2 424,013 - ABZR87_RS18090 0.62 +1.3 424,085 - ABZR87_RS18090 0.74 -0.2 424,168 + ABZR87_RS18090 0.87 +0.3 424,464 + ABZR87_RS18095 0.35 -0.3 424,631 + ABZR87_RS18095 0.60 -0.2 424,632 - ABZR87_RS18095 0.60 -0.5 424,632 - ABZR87_RS18095 0.60 +2.4 424,632 - ABZR87_RS18095 0.60 -0.7 424,632 - ABZR87_RS18095 0.60 -0.4 425,026 + ABZR87_RS18100 0.14 +0.1 425,026 + ABZR87_RS18100 0.14 -1.1 425,090 + ABZR87_RS18100 0.22 +0.2 425,169 - ABZR87_RS18100 0.32 +0.1 425,255 + ABZR87_RS18100 0.42 +1.2 425,311 + ABZR87_RS18100 0.49 +1.1 425,384 + ABZR87_RS18100 0.58 +0.1 425,384 + ABZR87_RS18100 0.58 -0.0 425,385 - ABZR87_RS18100 0.58 +1.2 425,391 - ABZR87_RS18100 0.59 +0.2 425,391 - ABZR87_RS18100 0.59 -0.7 425,840 + ABZR87_RS18105 0.27 -0.0 425,980 - ABZR87_RS18105 0.59 +0.0 426,236 + +0.6 426,236 + +0.0 426,237 - -2.2 426,254 + -0.1 426,254 + -1.3 426,254 + +0.6 426,254 + -0.2 426,254 + -0.5 426,254 + +0.1 426,255 - -0.0 426,255 - -0.1
Or see this region's nucleotide sequence