Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18075 and ABZR87_RS18080 overlap by 4 nucleotides ABZR87_RS18080 and ABZR87_RS18085 overlap by 38 nucleotides ABZR87_RS18085 and ABZR87_RS18090 overlap by 4 nucleotides ABZR87_RS18090 and ABZR87_RS18095 overlap by 19 nucleotides
ABZR87_RS18075: ABZR87_RS18075 - type III secretion system export apparatus subunit SctT, at 420,937 to 421,737
_RS18075
ABZR87_RS18080: ABZR87_RS18080 - hypothetical protein, at 421,734 to 422,246
_RS18080
ABZR87_RS18085: ABZR87_RS18085 - type III secretion system ATPase SctN, at 422,209 to 423,633
_RS18085
ABZR87_RS18090: ABZR87_RS18090 - type III secretion system stator protein SctL, at 423,630 to 424,244
_RS18090
ABZR87_RS18095: ABZR87_RS18095 - hypothetical protein, at 424,226 to 424,900
_RS18095
Position (kb) 422
423
424 Strain fitness (log2 ratio) -2
-1
0
1
2 at 421.440 kb on - strand, within ABZR87_RS18075 at 421.994 kb on + strand, within ABZR87_RS18080 at 421.995 kb on - strand, within ABZR87_RS18080 at 422.018 kb on - strand, within ABZR87_RS18080 at 422.180 kb on + strand, within ABZR87_RS18080 at 422.254 kb on + strand at 422.254 kb on + strand at 422.255 kb on - strand at 422.255 kb on - strand at 422.384 kb on + strand, within ABZR87_RS18085 at 422.384 kb on + strand, within ABZR87_RS18085 at 422.384 kb on + strand, within ABZR87_RS18085 at 422.385 kb on - strand, within ABZR87_RS18085 at 422.385 kb on - strand, within ABZR87_RS18085 at 422.385 kb on - strand, within ABZR87_RS18085 at 422.526 kb on - strand, within ABZR87_RS18085 at 422.623 kb on + strand, within ABZR87_RS18085 at 422.624 kb on - strand, within ABZR87_RS18085 at 422.636 kb on + strand, within ABZR87_RS18085 at 422.637 kb on - strand, within ABZR87_RS18085 at 422.637 kb on - strand, within ABZR87_RS18085 at 422.831 kb on + strand, within ABZR87_RS18085 at 422.832 kb on - strand, within ABZR87_RS18085 at 422.832 kb on - strand, within ABZR87_RS18085 at 422.832 kb on - strand, within ABZR87_RS18085 at 423.180 kb on - strand, within ABZR87_RS18085 at 423.378 kb on - strand, within ABZR87_RS18085 at 423.562 kb on + strand at 423.563 kb on - strand at 423.563 kb on - strand at 423.571 kb on + strand at 423.572 kb on - strand at 423.572 kb on - strand at 423.850 kb on + strand, within ABZR87_RS18090 at 424.012 kb on + strand, within ABZR87_RS18090 at 424.012 kb on + strand, within ABZR87_RS18090 at 424.013 kb on - strand, within ABZR87_RS18090 at 424.013 kb on - strand, within ABZR87_RS18090 at 424.013 kb on - strand, within ABZR87_RS18090 at 424.085 kb on - strand, within ABZR87_RS18090 at 424.168 kb on + strand, within ABZR87_RS18090 at 424.464 kb on + strand, within ABZR87_RS18095 at 424.631 kb on + strand, within ABZR87_RS18095 at 424.632 kb on - strand, within ABZR87_RS18095 at 424.632 kb on - strand, within ABZR87_RS18095 at 424.632 kb on - strand, within ABZR87_RS18095 at 424.632 kb on - strand, within ABZR87_RS18095
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 421,440 - ABZR87_RS18075 0.63 -0.3 421,994 + ABZR87_RS18080 0.51 -0.6 421,995 - ABZR87_RS18080 0.51 +0.8 422,018 - ABZR87_RS18080 0.55 -1.8 422,180 + ABZR87_RS18080 0.87 +1.5 422,254 + +0.6 422,254 + +0.7 422,255 - -0.7 422,255 - +0.1 422,384 + ABZR87_RS18085 0.12 -1.5 422,384 + ABZR87_RS18085 0.12 -0.0 422,384 + ABZR87_RS18085 0.12 -1.9 422,385 - ABZR87_RS18085 0.12 +0.6 422,385 - ABZR87_RS18085 0.12 +0.4 422,385 - ABZR87_RS18085 0.12 +0.1 422,526 - ABZR87_RS18085 0.22 -0.3 422,623 + ABZR87_RS18085 0.29 -0.1 422,624 - ABZR87_RS18085 0.29 -0.9 422,636 + ABZR87_RS18085 0.30 -0.2 422,637 - ABZR87_RS18085 0.30 +0.2 422,637 - ABZR87_RS18085 0.30 -1.8 422,831 + ABZR87_RS18085 0.44 +0.1 422,832 - ABZR87_RS18085 0.44 +0.8 422,832 - ABZR87_RS18085 0.44 -0.7 422,832 - ABZR87_RS18085 0.44 -0.7 423,180 - ABZR87_RS18085 0.68 -0.1 423,378 - ABZR87_RS18085 0.82 -0.4 423,562 + -1.7 423,563 - +0.8 423,563 - +0.4 423,571 + -0.5 423,572 - -0.8 423,572 - +0.8 423,850 + ABZR87_RS18090 0.36 -0.2 424,012 + ABZR87_RS18090 0.62 -1.3 424,012 + ABZR87_RS18090 0.62 +0.8 424,013 - ABZR87_RS18090 0.62 +0.3 424,013 - ABZR87_RS18090 0.62 -0.2 424,013 - ABZR87_RS18090 0.62 +1.3 424,085 - ABZR87_RS18090 0.74 -0.2 424,168 + ABZR87_RS18090 0.87 +0.3 424,464 + ABZR87_RS18095 0.35 -0.3 424,631 + ABZR87_RS18095 0.60 -0.2 424,632 - ABZR87_RS18095 0.60 -0.5 424,632 - ABZR87_RS18095 0.60 +2.4 424,632 - ABZR87_RS18095 0.60 -0.7 424,632 - ABZR87_RS18095 0.60 -0.4
Or see this region's nucleotide sequence