Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18020 and ABZR87_RS18025 are separated by 498 nucleotides ABZR87_RS18025 and ABZR87_RS18030 overlap by 4 nucleotides
ABZR87_RS18020: ABZR87_RS18020 - YitT family protein, at 398,565 to 399,218
_RS18020
ABZR87_RS18025: ABZR87_RS18025 - flagellar biosynthesis protein FlhA, at 399,717 to 401,852
_RS18025
ABZR87_RS18030: ABZR87_RS18030 - flagellar biosynthesis protein FlhF, at 401,849 to 403,756
_RS18030
Position (kb) 399
400
401
402 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 398.874 kb on + strand, within ABZR87_RS18020 at 398.874 kb on + strand, within ABZR87_RS18020 at 398.874 kb on + strand, within ABZR87_RS18020 at 398.875 kb on - strand, within ABZR87_RS18020 at 398.875 kb on - strand, within ABZR87_RS18020 at 398.875 kb on - strand, within ABZR87_RS18020 at 399.028 kb on + strand, within ABZR87_RS18020 at 399.029 kb on - strand, within ABZR87_RS18020 at 399.133 kb on + strand, within ABZR87_RS18020 at 399.134 kb on - strand, within ABZR87_RS18020 at 399.307 kb on + strand at 399.308 kb on - strand at 399.308 kb on - strand at 399.443 kb on - strand at 399.443 kb on - strand at 399.506 kb on + strand at 399.506 kb on + strand at 399.522 kb on - strand at 399.522 kb on - strand at 399.533 kb on + strand at 399.534 kb on - strand at 399.546 kb on + strand at 399.584 kb on + strand at 399.610 kb on + strand at 399.610 kb on + strand at 399.610 kb on + strand at 399.611 kb on - strand at 400.077 kb on + strand, within ABZR87_RS18025 at 400.077 kb on + strand, within ABZR87_RS18025 at 400.077 kb on + strand, within ABZR87_RS18025 at 400.078 kb on - strand, within ABZR87_RS18025 at 400.434 kb on + strand, within ABZR87_RS18025 at 400.434 kb on + strand, within ABZR87_RS18025 at 400.434 kb on + strand, within ABZR87_RS18025 at 400.434 kb on + strand, within ABZR87_RS18025 at 400.435 kb on - strand, within ABZR87_RS18025 at 400.435 kb on - strand, within ABZR87_RS18025 at 400.435 kb on - strand, within ABZR87_RS18025 at 400.674 kb on + strand, within ABZR87_RS18025 at 400.675 kb on - strand, within ABZR87_RS18025 at 401.199 kb on + strand, within ABZR87_RS18025 at 401.199 kb on + strand, within ABZR87_RS18025 at 401.199 kb on + strand, within ABZR87_RS18025 at 401.200 kb on - strand, within ABZR87_RS18025 at 401.200 kb on - strand, within ABZR87_RS18025 at 401.200 kb on - strand, within ABZR87_RS18025 at 401.253 kb on + strand, within ABZR87_RS18025 at 401.253 kb on + strand, within ABZR87_RS18025 at 401.254 kb on - strand, within ABZR87_RS18025 at 401.254 kb on - strand, within ABZR87_RS18025 at 401.261 kb on + strand, within ABZR87_RS18025 at 401.261 kb on + strand, within ABZR87_RS18025 at 401.261 kb on + strand, within ABZR87_RS18025 at 401.262 kb on - strand, within ABZR87_RS18025 at 401.262 kb on - strand, within ABZR87_RS18025 at 401.262 kb on - strand, within ABZR87_RS18025 at 401.414 kb on + strand, within ABZR87_RS18025 at 401.415 kb on - strand, within ABZR87_RS18025 at 401.615 kb on + strand, within ABZR87_RS18025 at 401.719 kb on + strand at 401.819 kb on + strand at 401.819 kb on + strand at 401.819 kb on + strand at 401.866 kb on + strand at 401.867 kb on - strand at 402.440 kb on - strand, within ABZR87_RS18030
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 398,874 + ABZR87_RS18020 0.47 -0.5 398,874 + ABZR87_RS18020 0.47 +1.1 398,874 + ABZR87_RS18020 0.47 +0.3 398,875 - ABZR87_RS18020 0.47 -0.2 398,875 - ABZR87_RS18020 0.47 -0.6 398,875 - ABZR87_RS18020 0.47 -0.3 399,028 + ABZR87_RS18020 0.71 -0.0 399,029 - ABZR87_RS18020 0.71 -0.8 399,133 + ABZR87_RS18020 0.87 +1.1 399,134 - ABZR87_RS18020 0.87 -0.8 399,307 + +0.4 399,308 - +0.4 399,308 - +0.7 399,443 - -0.6 399,443 - +2.0 399,506 + +0.6 399,506 + -0.5 399,522 - -0.6 399,522 - -0.0 399,533 + -0.6 399,534 - -0.3 399,546 + +1.2 399,584 + -1.5 399,610 + -0.4 399,610 + +0.2 399,610 + -0.6 399,611 - +0.5 400,077 + ABZR87_RS18025 0.17 +2.6 400,077 + ABZR87_RS18025 0.17 -0.9 400,077 + ABZR87_RS18025 0.17 +0.2 400,078 - ABZR87_RS18025 0.17 -0.4 400,434 + ABZR87_RS18025 0.34 -0.0 400,434 + ABZR87_RS18025 0.34 -2.5 400,434 + ABZR87_RS18025 0.34 -1.0 400,434 + ABZR87_RS18025 0.34 -0.1 400,435 - ABZR87_RS18025 0.34 -0.1 400,435 - ABZR87_RS18025 0.34 -0.9 400,435 - ABZR87_RS18025 0.34 +0.2 400,674 + ABZR87_RS18025 0.45 -2.2 400,675 - ABZR87_RS18025 0.45 -0.3 401,199 + ABZR87_RS18025 0.69 -1.4 401,199 + ABZR87_RS18025 0.69 -0.3 401,199 + ABZR87_RS18025 0.69 -1.4 401,200 - ABZR87_RS18025 0.69 -2.3 401,200 - ABZR87_RS18025 0.69 -3.0 401,200 - ABZR87_RS18025 0.69 -0.8 401,253 + ABZR87_RS18025 0.72 +1.4 401,253 + ABZR87_RS18025 0.72 -1.4 401,254 - ABZR87_RS18025 0.72 -1.7 401,254 - ABZR87_RS18025 0.72 -1.7 401,261 + ABZR87_RS18025 0.72 -0.9 401,261 + ABZR87_RS18025 0.72 -1.4 401,261 + ABZR87_RS18025 0.72 -0.4 401,262 - ABZR87_RS18025 0.72 -1.7 401,262 - ABZR87_RS18025 0.72 -0.1 401,262 - ABZR87_RS18025 0.72 -2.3 401,414 + ABZR87_RS18025 0.79 -1.3 401,415 - ABZR87_RS18025 0.79 -0.8 401,615 + ABZR87_RS18025 0.89 -0.6 401,719 + -1.3 401,819 + -0.9 401,819 + -0.6 401,819 + -2.2 401,866 + +0.8 401,867 - -0.2 402,440 - ABZR87_RS18030 0.31 -1.1
Or see this region's nucleotide sequence