Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS17815 and ABZR87_RS17820 overlap by 4 nucleotides ABZR87_RS17820 and ABZR87_RS17825 overlap by 4 nucleotides ABZR87_RS17825 and ABZR87_RS17830 overlap by 4 nucleotides ABZR87_RS17830 and ABZR87_RS17835 overlap by 23 nucleotides
ABZR87_RS17815: ABZR87_RS17815 - sterol desaturase family protein, at 356,080 to 357,213
_RS17815
ABZR87_RS17820: ABZR87_RS17820 - phosphatase PAP2 family protein, at 357,210 to 357,833
_RS17820
ABZR87_RS17825: ABZR87_RS17825 - F390 synthetase-related protein, at 357,830 to 359,140
_RS17825
ABZR87_RS17830: ABZR87_RS17830 - MBL fold metallo-hydrolase, at 359,137 to 359,943
_RS17830
ABZR87_RS17835: ABZR87_RS17835 - NAD(P)-dependent oxidoreductase, at 359,921 to 360,934
_RS17835
Position (kb)
357
358
359
360 Strain fitness (log2 ratio)
-1
0
1
2 at 357.030 kb on + strand, within ABZR87_RS17815 at 357.030 kb on + strand, within ABZR87_RS17815 at 357.030 kb on + strand, within ABZR87_RS17815 at 357.030 kb on + strand, within ABZR87_RS17815 at 357.031 kb on - strand, within ABZR87_RS17815 at 357.031 kb on - strand, within ABZR87_RS17815 at 357.031 kb on - strand, within ABZR87_RS17815 at 357.031 kb on - strand, within ABZR87_RS17815 at 357.031 kb on - strand, within ABZR87_RS17815 at 357.031 kb on - strand, within ABZR87_RS17815 at 357.031 kb on - strand, within ABZR87_RS17815 at 357.032 kb on + strand, within ABZR87_RS17815 at 357.032 kb on + strand, within ABZR87_RS17815 at 357.032 kb on + strand, within ABZR87_RS17815 at 357.033 kb on - strand, within ABZR87_RS17815 at 357.033 kb on - strand, within ABZR87_RS17815 at 357.033 kb on - strand, within ABZR87_RS17815 at 357.034 kb on + strand, within ABZR87_RS17815 at 357.034 kb on + strand, within ABZR87_RS17815 at 357.035 kb on - strand, within ABZR87_RS17815 at 357.261 kb on + strand at 357.262 kb on - strand at 357.262 kb on - strand at 357.640 kb on + strand, within ABZR87_RS17820 at 357.640 kb on + strand, within ABZR87_RS17820 at 357.673 kb on + strand, within ABZR87_RS17820 at 357.674 kb on - strand, within ABZR87_RS17820 at 357.674 kb on - strand, within ABZR87_RS17820 at 357.674 kb on - strand, within ABZR87_RS17820 at 357.674 kb on - strand, within ABZR87_RS17820 at 357.696 kb on - strand, within ABZR87_RS17820 at 357.769 kb on + strand, within ABZR87_RS17820 at 357.769 kb on + strand, within ABZR87_RS17820 at 357.770 kb on - strand, within ABZR87_RS17820 at 357.770 kb on - strand, within ABZR87_RS17820 at 357.770 kb on - strand, within ABZR87_RS17820 at 357.803 kb on + strand at 357.804 kb on - strand at 358.053 kb on + strand, within ABZR87_RS17825 at 358.150 kb on + strand, within ABZR87_RS17825 at 358.410 kb on + strand, within ABZR87_RS17825 at 358.410 kb on + strand, within ABZR87_RS17825 at 358.446 kb on - strand, within ABZR87_RS17825 at 358.487 kb on + strand, within ABZR87_RS17825 at 358.488 kb on - strand, within ABZR87_RS17825 at 358.668 kb on + strand, within ABZR87_RS17825 at 358.669 kb on - strand, within ABZR87_RS17825 at 358.669 kb on - strand, within ABZR87_RS17825 at 358.833 kb on + strand, within ABZR87_RS17825 at 358.834 kb on - strand, within ABZR87_RS17825 at 359.005 kb on - strand, within ABZR87_RS17825 at 359.110 kb on - strand at 359.219 kb on + strand, within ABZR87_RS17830 at 359.219 kb on + strand, within ABZR87_RS17830 at 359.219 kb on + strand, within ABZR87_RS17830 at 359.220 kb on - strand, within ABZR87_RS17830 at 359.279 kb on + strand, within ABZR87_RS17830 at 359.280 kb on - strand, within ABZR87_RS17830 at 359.727 kb on - strand, within ABZR87_RS17830 at 359.748 kb on - strand, within ABZR87_RS17830 at 359.901 kb on + strand at 359.901 kb on + strand at 359.902 kb on - strand at 360.004 kb on - strand at 360.049 kb on - strand, within ABZR87_RS17835
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 357,030 + ABZR87_RS17815 0.84 -0.1 357,030 + ABZR87_RS17815 0.84 +0.6 357,030 + ABZR87_RS17815 0.84 -0.6 357,030 + ABZR87_RS17815 0.84 +0.9 357,031 - ABZR87_RS17815 0.84 +0.2 357,031 - ABZR87_RS17815 0.84 +0.0 357,031 - ABZR87_RS17815 0.84 +1.1 357,031 - ABZR87_RS17815 0.84 -0.7 357,031 - ABZR87_RS17815 0.84 -0.2 357,031 - ABZR87_RS17815 0.84 -0.4 357,031 - ABZR87_RS17815 0.84 -0.3 357,032 + ABZR87_RS17815 0.84 -0.7 357,032 + ABZR87_RS17815 0.84 -0.5 357,032 + ABZR87_RS17815 0.84 -0.5 357,033 - ABZR87_RS17815 0.84 +0.0 357,033 - ABZR87_RS17815 0.84 +0.9 357,033 - ABZR87_RS17815 0.84 +1.0 357,034 + ABZR87_RS17815 0.84 +1.1 357,034 + ABZR87_RS17815 0.84 -0.5 357,035 - ABZR87_RS17815 0.84 +0.2 357,261 + -0.4 357,262 - +0.4 357,262 - -0.5 357,640 + ABZR87_RS17820 0.69 -0.0 357,640 + ABZR87_RS17820 0.69 +0.5 357,673 + ABZR87_RS17820 0.74 +0.9 357,674 - ABZR87_RS17820 0.74 +0.1 357,674 - ABZR87_RS17820 0.74 -1.5 357,674 - ABZR87_RS17820 0.74 -0.4 357,674 - ABZR87_RS17820 0.74 -1.1 357,696 - ABZR87_RS17820 0.78 -1.0 357,769 + ABZR87_RS17820 0.90 -0.3 357,769 + ABZR87_RS17820 0.90 -1.3 357,770 - ABZR87_RS17820 0.90 -0.0 357,770 - ABZR87_RS17820 0.90 +0.8 357,770 - ABZR87_RS17820 0.90 -0.1 357,803 + -0.8 357,804 - -0.2 358,053 + ABZR87_RS17825 0.17 +0.2 358,150 + ABZR87_RS17825 0.24 +0.3 358,410 + ABZR87_RS17825 0.44 -1.0 358,410 + ABZR87_RS17825 0.44 -1.3 358,446 - ABZR87_RS17825 0.47 -1.2 358,487 + ABZR87_RS17825 0.50 +1.1 358,488 - ABZR87_RS17825 0.50 +0.2 358,668 + ABZR87_RS17825 0.64 +0.6 358,669 - ABZR87_RS17825 0.64 +1.1 358,669 - ABZR87_RS17825 0.64 +0.7 358,833 + ABZR87_RS17825 0.77 +1.0 358,834 - ABZR87_RS17825 0.77 +1.8 359,005 - ABZR87_RS17825 0.90 +0.4 359,110 - +0.5 359,219 + ABZR87_RS17830 0.10 +0.5 359,219 + ABZR87_RS17830 0.10 -0.3 359,219 + ABZR87_RS17830 0.10 -0.4 359,220 - ABZR87_RS17830 0.10 +0.3 359,279 + ABZR87_RS17830 0.18 -1.0 359,280 - ABZR87_RS17830 0.18 -0.3 359,727 - ABZR87_RS17830 0.73 -1.3 359,748 - ABZR87_RS17830 0.76 +0.8 359,901 + +0.6 359,901 + -1.1 359,902 - -0.4 360,004 - -0.0 360,049 - ABZR87_RS17835 0.13 -0.6
Or see this region's nucleotide sequence