Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS17235

Experiment: Acanthamoeba sp., muxed culture-1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS17230 and ABZR87_RS17235 are separated by 403 nucleotidesABZR87_RS17235 and ABZR87_RS17240 are separated by 125 nucleotides ABZR87_RS17230: ABZR87_RS17230 - glycogen synthase GlgA, at 222,961 to 224,592 _RS17230 ABZR87_RS17235: ABZR87_RS17235 - alkyl hydroperoxide reductase subunit C, at 224,996 to 225,559 _RS17235 ABZR87_RS17240: ABZR87_RS17240 - alkyl hydroperoxide reductase subunit F, at 225,685 to 227,259 _RS17240 Position (kb) 224 225 226Strain fitness (log2 ratio) -2 -1 0 1 2at 224.089 kb on - strand, within ABZR87_RS17230at 224.089 kb on - strand, within ABZR87_RS17230at 224.097 kb on + strand, within ABZR87_RS17230at 224.098 kb on - strand, within ABZR87_RS17230at 224.098 kb on - strand, within ABZR87_RS17230at 224.264 kb on + strand, within ABZR87_RS17230at 224.289 kb on - strand, within ABZR87_RS17230at 224.517 kb on + strandat 224.517 kb on + strandat 224.518 kb on - strandat 224.518 kb on - strandat 224.518 kb on - strandat 224.518 kb on - strandat 224.696 kb on + strandat 224.696 kb on + strandat 224.696 kb on + strandat 224.697 kb on - strandat 224.697 kb on - strandat 224.697 kb on - strandat 224.697 kb on - strandat 224.697 kb on - strandat 224.776 kb on + strandat 224.815 kb on - strandat 224.815 kb on - strandat 224.824 kb on + strandat 224.824 kb on + strandat 224.916 kb on + strandat 224.950 kb on + strandat 224.950 kb on + strandat 224.995 kb on + strandat 224.995 kb on + strandat 224.995 kb on + strandat 224.995 kb on + strandat 224.995 kb on + strandat 224.995 kb on + strandat 224.995 kb on + strandat 224.995 kb on + strandat 224.995 kb on + strandat 224.996 kb on - strandat 225.010 kb on + strandat 225.010 kb on + strandat 225.010 kb on + strandat 225.011 kb on - strandat 225.011 kb on - strandat 225.011 kb on - strandat 225.167 kb on + strand, within ABZR87_RS17235at 225.167 kb on + strand, within ABZR87_RS17235at 225.167 kb on + strand, within ABZR87_RS17235at 225.168 kb on - strand, within ABZR87_RS17235at 225.168 kb on - strand, within ABZR87_RS17235at 225.168 kb on - strand, within ABZR87_RS17235at 225.200 kb on + strand, within ABZR87_RS17235at 225.200 kb on + strand, within ABZR87_RS17235at 225.200 kb on + strand, within ABZR87_RS17235at 225.201 kb on - strand, within ABZR87_RS17235at 225.201 kb on - strand, within ABZR87_RS17235at 225.201 kb on - strand, within ABZR87_RS17235at 225.557 kb on + strandat 225.557 kb on + strandat 225.558 kb on - strandat 225.558 kb on - strandat 225.558 kb on - strandat 225.558 kb on - strandat 225.558 kb on - strandat 225.565 kb on - strandat 225.565 kb on - strandat 225.625 kb on + strandat 225.626 kb on - strandat 225.626 kb on - strandat 225.671 kb on + strandat 225.672 kb on - strandat 225.721 kb on + strandat 225.722 kb on - strandat 225.982 kb on + strand, within ABZR87_RS17240at 225.982 kb on + strand, within ABZR87_RS17240at 225.983 kb on - strand, within ABZR87_RS17240at 225.983 kb on - strand, within ABZR87_RS17240at 226.027 kb on - strand, within ABZR87_RS17240at 226.054 kb on + strand, within ABZR87_RS17240at 226.054 kb on + strand, within ABZR87_RS17240at 226.054 kb on + strand, within ABZR87_RS17240at 226.054 kb on + strand, within ABZR87_RS17240at 226.054 kb on + strand, within ABZR87_RS17240at 226.054 kb on + strand, within ABZR87_RS17240at 226.054 kb on + strand, within ABZR87_RS17240at 226.055 kb on - strand, within ABZR87_RS17240at 226.055 kb on - strand, within ABZR87_RS17240at 226.055 kb on - strand, within ABZR87_RS17240at 226.160 kb on - strand, within ABZR87_RS17240at 226.160 kb on - strand, within ABZR87_RS17240at 226.160 kb on - strand, within ABZR87_RS17240at 226.372 kb on + strand, within ABZR87_RS17240

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Per-strain Table

Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1
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224,089 - ABZR87_RS17230 0.69 -0.2
224,089 - ABZR87_RS17230 0.69 +0.4
224,097 + ABZR87_RS17230 0.70 +0.8
224,098 - ABZR87_RS17230 0.70 +0.8
224,098 - ABZR87_RS17230 0.70 +0.1
224,264 + ABZR87_RS17230 0.80 +0.0
224,289 - ABZR87_RS17230 0.81 -0.5
224,517 + -0.4
224,517 + -1.3
224,518 - -0.2
224,518 - -0.1
224,518 - -0.5
224,518 - +1.3
224,696 + -0.0
224,696 + +0.3
224,696 + -0.8
224,697 - +0.4
224,697 - -0.4
224,697 - +0.2
224,697 - +0.3
224,697 - +0.0
224,776 + -0.0
224,815 - +0.1
224,815 - +2.2
224,824 + +1.2
224,824 + +0.4
224,916 + -0.4
224,950 + +0.3
224,950 + -2.6
224,995 + -0.9
224,995 + +0.2
224,995 + +1.2
224,995 + -0.2
224,995 + -0.4
224,995 + +1.1
224,995 + +1.0
224,995 + -0.8
224,995 + +0.4
224,996 - -0.7
225,010 + -1.9
225,010 + -0.3
225,010 + -0.2
225,011 - -1.8
225,011 - -2.3
225,011 - -2.0
225,167 + ABZR87_RS17235 0.30 -1.6
225,167 + ABZR87_RS17235 0.30 -0.2
225,167 + ABZR87_RS17235 0.30 -0.9
225,168 - ABZR87_RS17235 0.30 -1.1
225,168 - ABZR87_RS17235 0.30 +0.1
225,168 - ABZR87_RS17235 0.30 -0.6
225,200 + ABZR87_RS17235 0.36 +0.2
225,200 + ABZR87_RS17235 0.36 -1.8
225,200 + ABZR87_RS17235 0.36 -0.2
225,201 - ABZR87_RS17235 0.36 -0.5
225,201 - ABZR87_RS17235 0.36 -0.2
225,201 - ABZR87_RS17235 0.36 -1.0
225,557 + -1.6
225,557 + +1.9
225,558 - -0.7
225,558 - +0.1
225,558 - -0.3
225,558 - +0.6
225,558 - +1.4
225,565 - -0.6
225,565 - +0.5
225,625 + -1.7
225,626 - -0.9
225,626 - +0.9
225,671 + -0.7
225,672 - -0.4
225,721 + -0.6
225,722 - -1.6
225,982 + ABZR87_RS17240 0.19 -0.1
225,982 + ABZR87_RS17240 0.19 -0.1
225,983 - ABZR87_RS17240 0.19 +0.8
225,983 - ABZR87_RS17240 0.19 -2.0
226,027 - ABZR87_RS17240 0.22 -0.9
226,054 + ABZR87_RS17240 0.23 +0.9
226,054 + ABZR87_RS17240 0.23 +1.2
226,054 + ABZR87_RS17240 0.23 +0.0
226,054 + ABZR87_RS17240 0.23 +0.6
226,054 + ABZR87_RS17240 0.23 -1.0
226,054 + ABZR87_RS17240 0.23 +0.1
226,054 + ABZR87_RS17240 0.23 +0.5
226,055 - ABZR87_RS17240 0.23 -0.2
226,055 - ABZR87_RS17240 0.23 -0.1
226,055 - ABZR87_RS17240 0.23 -0.1
226,160 - ABZR87_RS17240 0.30 -0.8
226,160 - ABZR87_RS17240 0.30 -0.4
226,160 - ABZR87_RS17240 0.30 +0.1
226,372 + ABZR87_RS17240 0.44 +0.4

Or see this region's nucleotide sequence