Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS17030 and ABZR87_RS17035 are separated by 122 nucleotides ABZR87_RS17035 and ABZR87_RS17040 are separated by 456 nucleotides ABZR87_RS17040 and ABZR87_RS17045 are separated by 20 nucleotides
ABZR87_RS17030: ABZR87_RS17030 - ionic transporter y4hA, at 171,671 to 172,753
_RS17030
ABZR87_RS17035: ABZR87_RS17035 - spherulation-specific family 4 protein, at 172,876 to 173,730
_RS17035
ABZR87_RS17040: ABZR87_RS17040 - hypothetical protein, at 174,187 to 174,642
_RS17040
ABZR87_RS17045: ABZR87_RS17045 - LysR family transcriptional regulator, at 174,663 to 175,556
_RS17045
Position (kb) 172
173
174 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 172.176 kb on + strand, within ABZR87_RS17030 at 172.177 kb on - strand, within ABZR87_RS17030 at 172.218 kb on + strand, within ABZR87_RS17030 at 172.218 kb on + strand, within ABZR87_RS17030 at 172.218 kb on + strand, within ABZR87_RS17030 at 172.219 kb on - strand, within ABZR87_RS17030 at 172.293 kb on + strand, within ABZR87_RS17030 at 172.293 kb on + strand, within ABZR87_RS17030 at 172.294 kb on - strand, within ABZR87_RS17030 at 172.294 kb on - strand, within ABZR87_RS17030 at 172.294 kb on - strand, within ABZR87_RS17030 at 172.819 kb on + strand at 172.820 kb on - strand at 172.821 kb on + strand at 172.821 kb on + strand at 172.821 kb on + strand at 172.821 kb on + strand at 172.821 kb on + strand at 172.821 kb on + strand at 172.821 kb on + strand at 172.822 kb on - strand at 172.822 kb on - strand at 172.822 kb on - strand at 172.822 kb on - strand at 172.916 kb on + strand at 172.916 kb on + strand at 173.033 kb on + strand, within ABZR87_RS17035 at 173.063 kb on + strand, within ABZR87_RS17035 at 173.090 kb on + strand, within ABZR87_RS17035 at 173.090 kb on + strand, within ABZR87_RS17035 at 173.090 kb on + strand, within ABZR87_RS17035 at 173.091 kb on - strand, within ABZR87_RS17035 at 173.091 kb on - strand, within ABZR87_RS17035 at 173.091 kb on - strand, within ABZR87_RS17035 at 173.091 kb on - strand, within ABZR87_RS17035 at 173.099 kb on + strand, within ABZR87_RS17035 at 173.099 kb on + strand, within ABZR87_RS17035 at 173.100 kb on - strand, within ABZR87_RS17035 at 173.100 kb on - strand, within ABZR87_RS17035 at 173.156 kb on + strand, within ABZR87_RS17035 at 173.157 kb on - strand, within ABZR87_RS17035 at 173.157 kb on - strand, within ABZR87_RS17035 at 173.157 kb on - strand, within ABZR87_RS17035 at 173.198 kb on + strand, within ABZR87_RS17035 at 173.198 kb on + strand, within ABZR87_RS17035 at 173.198 kb on + strand, within ABZR87_RS17035 at 173.199 kb on - strand, within ABZR87_RS17035 at 173.199 kb on - strand, within ABZR87_RS17035 at 173.199 kb on - strand, within ABZR87_RS17035 at 173.199 kb on - strand, within ABZR87_RS17035 at 173.222 kb on + strand, within ABZR87_RS17035 at 173.222 kb on + strand, within ABZR87_RS17035 at 173.222 kb on + strand, within ABZR87_RS17035 at 173.222 kb on + strand, within ABZR87_RS17035 at 173.222 kb on + strand, within ABZR87_RS17035 at 173.222 kb on + strand, within ABZR87_RS17035 at 173.222 kb on + strand, within ABZR87_RS17035 at 173.223 kb on - strand, within ABZR87_RS17035 at 173.223 kb on - strand, within ABZR87_RS17035 at 173.223 kb on - strand, within ABZR87_RS17035 at 173.223 kb on - strand, within ABZR87_RS17035 at 173.223 kb on - strand, within ABZR87_RS17035 at 173.223 kb on - strand, within ABZR87_RS17035 at 173.228 kb on + strand, within ABZR87_RS17035 at 173.240 kb on + strand, within ABZR87_RS17035 at 173.240 kb on + strand, within ABZR87_RS17035 at 173.240 kb on + strand, within ABZR87_RS17035 at 173.240 kb on + strand, within ABZR87_RS17035 at 173.306 kb on + strand, within ABZR87_RS17035 at 173.306 kb on + strand, within ABZR87_RS17035 at 173.307 kb on - strand, within ABZR87_RS17035 at 173.318 kb on + strand, within ABZR87_RS17035 at 173.318 kb on + strand, within ABZR87_RS17035 at 173.318 kb on + strand, within ABZR87_RS17035 at 173.364 kb on - strand, within ABZR87_RS17035 at 173.379 kb on - strand, within ABZR87_RS17035 at 173.379 kb on - strand, within ABZR87_RS17035 at 173.429 kb on + strand, within ABZR87_RS17035 at 173.429 kb on + strand, within ABZR87_RS17035 at 173.429 kb on + strand, within ABZR87_RS17035 at 173.429 kb on + strand, within ABZR87_RS17035 at 173.429 kb on + strand, within ABZR87_RS17035 at 173.429 kb on + strand, within ABZR87_RS17035 at 173.429 kb on + strand, within ABZR87_RS17035 at 173.429 kb on + strand, within ABZR87_RS17035 at 173.429 kb on + strand, within ABZR87_RS17035 at 173.429 kb on + strand, within ABZR87_RS17035 at 173.430 kb on - strand, within ABZR87_RS17035 at 173.430 kb on - strand, within ABZR87_RS17035 at 173.430 kb on - strand, within ABZR87_RS17035 at 173.430 kb on - strand, within ABZR87_RS17035 at 173.430 kb on - strand, within ABZR87_RS17035 at 173.430 kb on - strand, within ABZR87_RS17035 at 173.546 kb on + strand, within ABZR87_RS17035 at 173.547 kb on - strand, within ABZR87_RS17035 at 173.547 kb on - strand, within ABZR87_RS17035 at 173.547 kb on - strand, within ABZR87_RS17035 at 173.552 kb on + strand, within ABZR87_RS17035 at 173.552 kb on + strand, within ABZR87_RS17035 at 173.552 kb on + strand, within ABZR87_RS17035 at 173.552 kb on + strand, within ABZR87_RS17035 at 173.552 kb on + strand, within ABZR87_RS17035 at 173.552 kb on + strand, within ABZR87_RS17035 at 173.553 kb on - strand, within ABZR87_RS17035 at 173.553 kb on - strand, within ABZR87_RS17035 at 173.553 kb on - strand, within ABZR87_RS17035 at 173.553 kb on - strand, within ABZR87_RS17035 at 173.553 kb on - strand, within ABZR87_RS17035 at 173.631 kb on - strand, within ABZR87_RS17035 at 173.828 kb on + strand at 173.828 kb on + strand at 173.828 kb on + strand at 173.828 kb on + strand at 173.828 kb on + strand at 173.829 kb on - strand at 173.830 kb on + strand at 173.830 kb on + strand at 173.830 kb on + strand at 173.831 kb on - strand at 173.831 kb on - strand at 173.831 kb on - strand at 173.831 kb on - strand at 174.164 kb on + strand at 174.164 kb on + strand at 174.164 kb on + strand at 174.164 kb on + strand at 174.165 kb on - strand at 174.165 kb on - strand at 174.165 kb on - strand at 174.326 kb on - strand, within ABZR87_RS17040 at 174.326 kb on - strand, within ABZR87_RS17040 at 174.358 kb on + strand, within ABZR87_RS17040 at 174.358 kb on + strand, within ABZR87_RS17040 at 174.359 kb on - strand, within ABZR87_RS17040 at 174.410 kb on - strand, within ABZR87_RS17040 at 174.410 kb on - strand, within ABZR87_RS17040 at 174.410 kb on - strand, within ABZR87_RS17040 at 174.410 kb on - strand, within ABZR87_RS17040 at 174.410 kb on - strand, within ABZR87_RS17040 at 174.469 kb on + strand, within ABZR87_RS17040 at 174.469 kb on + strand, within ABZR87_RS17040 at 174.469 kb on + strand, within ABZR87_RS17040 at 174.469 kb on + strand, within ABZR87_RS17040 at 174.469 kb on + strand, within ABZR87_RS17040 at 174.470 kb on - strand, within ABZR87_RS17040 at 174.470 kb on - strand, within ABZR87_RS17040 at 174.470 kb on - strand, within ABZR87_RS17040 at 174.470 kb on - strand, within ABZR87_RS17040 at 174.589 kb on + strand, within ABZR87_RS17040 at 174.589 kb on + strand, within ABZR87_RS17040 at 174.590 kb on - strand, within ABZR87_RS17040
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 172,176 + ABZR87_RS17030 0.47 -1.4 172,177 - ABZR87_RS17030 0.47 +0.0 172,218 + ABZR87_RS17030 0.51 -0.6 172,218 + ABZR87_RS17030 0.51 +0.2 172,218 + ABZR87_RS17030 0.51 -0.0 172,219 - ABZR87_RS17030 0.51 -1.1 172,293 + ABZR87_RS17030 0.57 -0.8 172,293 + ABZR87_RS17030 0.57 -0.2 172,294 - ABZR87_RS17030 0.58 +0.7 172,294 - ABZR87_RS17030 0.58 -0.2 172,294 - ABZR87_RS17030 0.58 +0.0 172,819 + -1.5 172,820 - +0.2 172,821 + -0.9 172,821 + -0.7 172,821 + -0.5 172,821 + +0.6 172,821 + -0.4 172,821 + -1.5 172,821 + +0.4 172,822 - -0.1 172,822 - -0.9 172,822 - +0.2 172,822 - -0.5 172,916 + +0.7 172,916 + +0.1 173,033 + ABZR87_RS17035 0.18 +0.2 173,063 + ABZR87_RS17035 0.22 +0.1 173,090 + ABZR87_RS17035 0.25 -0.5 173,090 + ABZR87_RS17035 0.25 +0.7 173,090 + ABZR87_RS17035 0.25 -0.6 173,091 - ABZR87_RS17035 0.25 +0.5 173,091 - ABZR87_RS17035 0.25 +0.5 173,091 - ABZR87_RS17035 0.25 +0.6 173,091 - ABZR87_RS17035 0.25 +0.1 173,099 + ABZR87_RS17035 0.26 +0.2 173,099 + ABZR87_RS17035 0.26 -0.3 173,100 - ABZR87_RS17035 0.26 +0.1 173,100 - ABZR87_RS17035 0.26 -0.2 173,156 + ABZR87_RS17035 0.33 +2.3 173,157 - ABZR87_RS17035 0.33 +0.6 173,157 - ABZR87_RS17035 0.33 +0.9 173,157 - ABZR87_RS17035 0.33 -0.3 173,198 + ABZR87_RS17035 0.38 +1.1 173,198 + ABZR87_RS17035 0.38 +0.7 173,198 + ABZR87_RS17035 0.38 +0.4 173,199 - ABZR87_RS17035 0.38 -0.9 173,199 - ABZR87_RS17035 0.38 +1.9 173,199 - ABZR87_RS17035 0.38 +1.6 173,199 - ABZR87_RS17035 0.38 +0.2 173,222 + ABZR87_RS17035 0.40 -0.3 173,222 + ABZR87_RS17035 0.40 -0.7 173,222 + ABZR87_RS17035 0.40 -0.3 173,222 + ABZR87_RS17035 0.40 -0.2 173,222 + ABZR87_RS17035 0.40 -1.0 173,222 + ABZR87_RS17035 0.40 +0.7 173,222 + ABZR87_RS17035 0.40 -0.6 173,223 - ABZR87_RS17035 0.41 -0.6 173,223 - ABZR87_RS17035 0.41 -1.0 173,223 - ABZR87_RS17035 0.41 +0.7 173,223 - ABZR87_RS17035 0.41 +0.9 173,223 - ABZR87_RS17035 0.41 +1.1 173,223 - ABZR87_RS17035 0.41 +1.1 173,228 + ABZR87_RS17035 0.41 -0.3 173,240 + ABZR87_RS17035 0.43 +1.4 173,240 + ABZR87_RS17035 0.43 -0.0 173,240 + ABZR87_RS17035 0.43 -0.2 173,240 + ABZR87_RS17035 0.43 -0.3 173,306 + ABZR87_RS17035 0.50 -0.0 173,306 + ABZR87_RS17035 0.50 -0.1 173,307 - ABZR87_RS17035 0.50 +0.1 173,318 + ABZR87_RS17035 0.52 -1.9 173,318 + ABZR87_RS17035 0.52 -1.8 173,318 + ABZR87_RS17035 0.52 -0.4 173,364 - ABZR87_RS17035 0.57 -0.4 173,379 - ABZR87_RS17035 0.59 +1.1 173,379 - ABZR87_RS17035 0.59 +0.1 173,429 + ABZR87_RS17035 0.65 -0.4 173,429 + ABZR87_RS17035 0.65 +0.2 173,429 + ABZR87_RS17035 0.65 +0.3 173,429 + ABZR87_RS17035 0.65 +0.2 173,429 + ABZR87_RS17035 0.65 -0.4 173,429 + ABZR87_RS17035 0.65 +0.1 173,429 + ABZR87_RS17035 0.65 +0.7 173,429 + ABZR87_RS17035 0.65 -0.2 173,429 + ABZR87_RS17035 0.65 +0.4 173,429 + ABZR87_RS17035 0.65 +0.1 173,430 - ABZR87_RS17035 0.65 +0.1 173,430 - ABZR87_RS17035 0.65 +0.2 173,430 - ABZR87_RS17035 0.65 +0.3 173,430 - ABZR87_RS17035 0.65 +1.1 173,430 - ABZR87_RS17035 0.65 -0.2 173,430 - ABZR87_RS17035 0.65 +0.4 173,546 + ABZR87_RS17035 0.78 -0.1 173,547 - ABZR87_RS17035 0.78 -0.9 173,547 - ABZR87_RS17035 0.78 +0.7 173,547 - ABZR87_RS17035 0.78 -1.1 173,552 + ABZR87_RS17035 0.79 -0.8 173,552 + ABZR87_RS17035 0.79 -0.9 173,552 + ABZR87_RS17035 0.79 +0.8 173,552 + ABZR87_RS17035 0.79 +0.2 173,552 + ABZR87_RS17035 0.79 -0.0 173,552 + ABZR87_RS17035 0.79 -0.9 173,553 - ABZR87_RS17035 0.79 -1.2 173,553 - ABZR87_RS17035 0.79 +1.4 173,553 - ABZR87_RS17035 0.79 +0.8 173,553 - ABZR87_RS17035 0.79 -0.8 173,553 - ABZR87_RS17035 0.79 +0.4 173,631 - ABZR87_RS17035 0.88 -0.4 173,828 + +0.0 173,828 + -0.4 173,828 + +0.9 173,828 + +1.4 173,828 + -2.8 173,829 - -0.8 173,830 + -1.8 173,830 + -0.8 173,830 + -0.1 173,831 - -0.1 173,831 - +0.3 173,831 - -0.3 173,831 - -1.5 174,164 + +0.7 174,164 + -0.5 174,164 + +0.1 174,164 + +0.1 174,165 - +1.5 174,165 - +0.9 174,165 - +0.7 174,326 - ABZR87_RS17040 0.30 -0.4 174,326 - ABZR87_RS17040 0.30 -0.1 174,358 + ABZR87_RS17040 0.38 -0.4 174,358 + ABZR87_RS17040 0.38 -0.0 174,359 - ABZR87_RS17040 0.38 +0.9 174,410 - ABZR87_RS17040 0.49 +1.2 174,410 - ABZR87_RS17040 0.49 -0.2 174,410 - ABZR87_RS17040 0.49 -0.0 174,410 - ABZR87_RS17040 0.49 +0.1 174,410 - ABZR87_RS17040 0.49 -0.6 174,469 + ABZR87_RS17040 0.62 -0.1 174,469 + ABZR87_RS17040 0.62 +0.4 174,469 + ABZR87_RS17040 0.62 -1.1 174,469 + ABZR87_RS17040 0.62 -0.3 174,469 + ABZR87_RS17040 0.62 +0.6 174,470 - ABZR87_RS17040 0.62 -0.6 174,470 - ABZR87_RS17040 0.62 -0.9 174,470 - ABZR87_RS17040 0.62 +0.2 174,470 - ABZR87_RS17040 0.62 -0.8 174,589 + ABZR87_RS17040 0.88 -0.1 174,589 + ABZR87_RS17040 0.88 -0.8 174,590 - ABZR87_RS17040 0.88 +0.4
Or see this region's nucleotide sequence