Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16850 and ABZR87_RS16855 are separated by 39 nucleotides ABZR87_RS16855 and ABZR87_RS16860 overlap by 8 nucleotides ABZR87_RS16860 and ABZR87_RS16865 are separated by 33 nucleotides
ABZR87_RS16850: ABZR87_RS16850 - HD domain-containing phosphohydrolase, at 135,051 to 136,436
_RS16850
ABZR87_RS16855: ABZR87_RS16855 - chemotaxis protein CheC, at 136,476 to 137,087
_RS16855
ABZR87_RS16860: ABZR87_RS16860 - response regulator, at 137,080 to 137,448
_RS16860
ABZR87_RS16865: ABZR87_RS16865 - ATP-binding protein, at 137,482 to 138,867
_RS16865
Position (kb)
137
138 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 136.223 kb on + strand, within ABZR87_RS16850 at 136.224 kb on - strand, within ABZR87_RS16850 at 136.224 kb on - strand, within ABZR87_RS16850 at 136.257 kb on + strand, within ABZR87_RS16850 at 136.302 kb on + strand at 136.427 kb on - strand at 136.427 kb on - strand at 136.427 kb on - strand at 136.605 kb on + strand, within ABZR87_RS16855 at 136.605 kb on + strand, within ABZR87_RS16855 at 136.716 kb on - strand, within ABZR87_RS16855 at 136.716 kb on - strand, within ABZR87_RS16855 at 136.716 kb on - strand, within ABZR87_RS16855 at 136.793 kb on - strand, within ABZR87_RS16855 at 136.793 kb on - strand, within ABZR87_RS16855 at 136.793 kb on - strand, within ABZR87_RS16855 at 137.087 kb on + strand at 137.087 kb on + strand at 137.087 kb on + strand at 137.087 kb on + strand at 137.088 kb on - strand at 137.088 kb on - strand at 137.088 kb on - strand at 137.096 kb on + strand at 137.097 kb on - strand at 137.244 kb on + strand, within ABZR87_RS16860 at 137.244 kb on + strand, within ABZR87_RS16860 at 137.245 kb on - strand, within ABZR87_RS16860 at 137.468 kb on + strand at 137.469 kb on - strand at 137.472 kb on + strand at 137.472 kb on + strand at 137.473 kb on - strand at 137.473 kb on - strand at 137.473 kb on - strand at 137.552 kb on - strand at 137.552 kb on - strand at 137.627 kb on + strand, within ABZR87_RS16865 at 137.627 kb on + strand, within ABZR87_RS16865 at 137.627 kb on + strand, within ABZR87_RS16865 at 137.627 kb on + strand, within ABZR87_RS16865 at 137.627 kb on + strand, within ABZR87_RS16865 at 137.676 kb on + strand, within ABZR87_RS16865 at 137.676 kb on + strand, within ABZR87_RS16865 at 137.676 kb on + strand, within ABZR87_RS16865 at 137.677 kb on - strand, within ABZR87_RS16865 at 137.677 kb on - strand, within ABZR87_RS16865 at 137.677 kb on - strand, within ABZR87_RS16865 at 137.717 kb on - strand, within ABZR87_RS16865 at 137.717 kb on - strand, within ABZR87_RS16865 at 137.831 kb on + strand, within ABZR87_RS16865 at 137.831 kb on + strand, within ABZR87_RS16865 at 137.831 kb on + strand, within ABZR87_RS16865 at 137.831 kb on + strand, within ABZR87_RS16865 at 137.831 kb on + strand, within ABZR87_RS16865 at 137.831 kb on + strand, within ABZR87_RS16865 at 137.831 kb on + strand, within ABZR87_RS16865 at 137.831 kb on + strand, within ABZR87_RS16865 at 137.831 kb on + strand, within ABZR87_RS16865 at 137.832 kb on - strand, within ABZR87_RS16865 at 137.832 kb on - strand, within ABZR87_RS16865 at 137.832 kb on - strand, within ABZR87_RS16865 at 137.832 kb on - strand, within ABZR87_RS16865 at 137.889 kb on + strand, within ABZR87_RS16865 at 137.889 kb on + strand, within ABZR87_RS16865 at 137.889 kb on + strand, within ABZR87_RS16865 at 137.890 kb on - strand, within ABZR87_RS16865 at 137.890 kb on - strand, within ABZR87_RS16865 at 137.890 kb on - strand, within ABZR87_RS16865 at 137.915 kb on + strand, within ABZR87_RS16865 at 137.916 kb on - strand, within ABZR87_RS16865 at 137.939 kb on + strand, within ABZR87_RS16865 at 137.940 kb on - strand, within ABZR87_RS16865 at 137.940 kb on - strand, within ABZR87_RS16865 at 138.023 kb on - strand, within ABZR87_RS16865 at 138.023 kb on - strand, within ABZR87_RS16865 at 138.091 kb on + strand, within ABZR87_RS16865 at 138.209 kb on + strand, within ABZR87_RS16865 at 138.209 kb on + strand, within ABZR87_RS16865 at 138.210 kb on - strand, within ABZR87_RS16865 at 138.230 kb on + strand, within ABZR87_RS16865 at 138.230 kb on + strand, within ABZR87_RS16865 at 138.230 kb on + strand, within ABZR87_RS16865 at 138.231 kb on - strand, within ABZR87_RS16865 at 138.231 kb on - strand, within ABZR87_RS16865 at 138.231 kb on - strand, within ABZR87_RS16865
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 136,223 + ABZR87_RS16850 0.85 +1.3 136,224 - ABZR87_RS16850 0.85 -0.3 136,224 - ABZR87_RS16850 0.85 +0.2 136,257 + ABZR87_RS16850 0.87 -1.2 136,302 + +0.6 136,427 - +0.4 136,427 - -0.9 136,427 - -0.8 136,605 + ABZR87_RS16855 0.21 -1.2 136,605 + ABZR87_RS16855 0.21 -0.4 136,716 - ABZR87_RS16855 0.39 -0.7 136,716 - ABZR87_RS16855 0.39 +0.1 136,716 - ABZR87_RS16855 0.39 -0.2 136,793 - ABZR87_RS16855 0.52 -1.5 136,793 - ABZR87_RS16855 0.52 -0.1 136,793 - ABZR87_RS16855 0.52 +0.9 137,087 + +0.4 137,087 + +0.7 137,087 + -0.3 137,087 + -1.0 137,088 - +0.2 137,088 - -1.5 137,088 - -1.3 137,096 + +0.2 137,097 - -3.2 137,244 + ABZR87_RS16860 0.44 -1.1 137,244 + ABZR87_RS16860 0.44 -1.4 137,245 - ABZR87_RS16860 0.45 -1.4 137,468 + -0.9 137,469 - +0.4 137,472 + -0.0 137,472 + -2.2 137,473 - +0.8 137,473 - -0.3 137,473 - +1.0 137,552 - -1.2 137,552 - -0.6 137,627 + ABZR87_RS16865 0.10 -0.2 137,627 + ABZR87_RS16865 0.10 +0.9 137,627 + ABZR87_RS16865 0.10 +0.6 137,627 + ABZR87_RS16865 0.10 -0.3 137,627 + ABZR87_RS16865 0.10 +0.7 137,676 + ABZR87_RS16865 0.14 +0.1 137,676 + ABZR87_RS16865 0.14 -0.1 137,676 + ABZR87_RS16865 0.14 -0.1 137,677 - ABZR87_RS16865 0.14 -0.5 137,677 - ABZR87_RS16865 0.14 -2.0 137,677 - ABZR87_RS16865 0.14 -0.8 137,717 - ABZR87_RS16865 0.17 +0.9 137,717 - ABZR87_RS16865 0.17 -0.9 137,831 + ABZR87_RS16865 0.25 +1.0 137,831 + ABZR87_RS16865 0.25 -0.8 137,831 + ABZR87_RS16865 0.25 -0.2 137,831 + ABZR87_RS16865 0.25 +0.0 137,831 + ABZR87_RS16865 0.25 -0.4 137,831 + ABZR87_RS16865 0.25 -0.1 137,831 + ABZR87_RS16865 0.25 +0.3 137,831 + ABZR87_RS16865 0.25 -0.2 137,831 + ABZR87_RS16865 0.25 -0.4 137,832 - ABZR87_RS16865 0.25 -3.6 137,832 - ABZR87_RS16865 0.25 -0.1 137,832 - ABZR87_RS16865 0.25 -0.4 137,832 - ABZR87_RS16865 0.25 -0.8 137,889 + ABZR87_RS16865 0.29 +0.8 137,889 + ABZR87_RS16865 0.29 -0.3 137,889 + ABZR87_RS16865 0.29 -0.6 137,890 - ABZR87_RS16865 0.29 -0.5 137,890 - ABZR87_RS16865 0.29 -0.8 137,890 - ABZR87_RS16865 0.29 -0.9 137,915 + ABZR87_RS16865 0.31 -0.8 137,916 - ABZR87_RS16865 0.31 +0.0 137,939 + ABZR87_RS16865 0.33 -0.9 137,940 - ABZR87_RS16865 0.33 +0.4 137,940 - ABZR87_RS16865 0.33 +1.7 138,023 - ABZR87_RS16865 0.39 -0.1 138,023 - ABZR87_RS16865 0.39 +0.1 138,091 + ABZR87_RS16865 0.44 -0.6 138,209 + ABZR87_RS16865 0.52 -1.2 138,209 + ABZR87_RS16865 0.52 +0.3 138,210 - ABZR87_RS16865 0.53 +0.5 138,230 + ABZR87_RS16865 0.54 -0.4 138,230 + ABZR87_RS16865 0.54 -0.6 138,230 + ABZR87_RS16865 0.54 +0.3 138,231 - ABZR87_RS16865 0.54 +0.3 138,231 - ABZR87_RS16865 0.54 +0.0 138,231 - ABZR87_RS16865 0.54 -1.9
Or see this region's nucleotide sequence