Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS15500 and ABZR87_RS15505 are separated by 83 nucleotides ABZR87_RS15505 and ABZR87_RS15510 are separated by 150 nucleotides ABZR87_RS15510 and ABZR87_RS15515 overlap by 4 nucleotides
ABZR87_RS15500: ABZR87_RS15500 - glycosyltransferase, at 3,262,705 to 3,263,772
_RS15500
ABZR87_RS15505: ABZR87_RS15505 - DNA polymerase III subunit alpha, at 3,263,856 to 3,267,374
_RS15505
ABZR87_RS15510: ABZR87_RS15510 - sulfurtransferase, at 3,267,525 to 3,268,373
_RS15510
ABZR87_RS15515: ABZR87_RS15515 - hypothetical protein, at 3,268,370 to 3,268,495
_RS15515
Position (kb)
3263
3264
3265
3266
3267
3268 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3262.884 kb on + strand, within ABZR87_RS15500 at 3262.923 kb on + strand, within ABZR87_RS15500 at 3262.924 kb on - strand, within ABZR87_RS15500 at 3262.992 kb on - strand, within ABZR87_RS15500 at 3263.299 kb on + strand, within ABZR87_RS15500 at 3263.300 kb on - strand, within ABZR87_RS15500 at 3263.321 kb on + strand, within ABZR87_RS15500 at 3263.322 kb on - strand, within ABZR87_RS15500 at 3263.324 kb on + strand, within ABZR87_RS15500 at 3263.384 kb on + strand, within ABZR87_RS15500 at 3263.385 kb on - strand, within ABZR87_RS15500 at 3263.385 kb on - strand, within ABZR87_RS15500 at 3263.385 kb on - strand, within ABZR87_RS15500 at 3263.453 kb on + strand, within ABZR87_RS15500 at 3263.466 kb on + strand, within ABZR87_RS15500 at 3263.478 kb on + strand, within ABZR87_RS15500 at 3263.478 kb on + strand, within ABZR87_RS15500 at 3263.478 kb on + strand, within ABZR87_RS15500 at 3263.478 kb on + strand, within ABZR87_RS15500 at 3263.478 kb on + strand, within ABZR87_RS15500 at 3263.479 kb on - strand, within ABZR87_RS15500 at 3263.546 kb on + strand, within ABZR87_RS15500 at 3263.739 kb on - strand at 3263.781 kb on + strand at 3263.781 kb on + strand at 3263.781 kb on + strand at 3263.782 kb on - strand at 3263.787 kb on + strand at 3263.787 kb on + strand at 3263.793 kb on + strand at 3263.794 kb on - strand at 3263.857 kb on + strand at 3263.858 kb on - strand at 3263.858 kb on - strand at 3263.858 kb on - strand at 3263.858 kb on - strand at 3263.863 kb on + strand at 3263.863 kb on + strand at 3263.864 kb on - strand at 3263.864 kb on - strand at 3263.864 kb on - strand at 3264.363 kb on - strand, within ABZR87_RS15505 at 3267.386 kb on - strand at 3267.464 kb on + strand at 3267.467 kb on - strand at 3267.478 kb on - strand at 3267.539 kb on + strand at 3267.540 kb on - strand at 3267.540 kb on - strand at 3267.549 kb on + strand at 3267.550 kb on - strand at 3267.550 kb on - strand at 3267.945 kb on + strand, within ABZR87_RS15510 at 3267.945 kb on + strand, within ABZR87_RS15510 at 3267.945 kb on + strand, within ABZR87_RS15510 at 3267.946 kb on - strand, within ABZR87_RS15510 at 3267.946 kb on - strand, within ABZR87_RS15510 at 3267.946 kb on - strand, within ABZR87_RS15510 at 3267.984 kb on + strand, within ABZR87_RS15510 at 3267.985 kb on - strand, within ABZR87_RS15510 at 3267.985 kb on - strand, within ABZR87_RS15510 at 3267.985 kb on - strand, within ABZR87_RS15510 at 3267.985 kb on - strand, within ABZR87_RS15510 at 3267.985 kb on - strand, within ABZR87_RS15510 at 3267.985 kb on - strand, within ABZR87_RS15510 at 3268.135 kb on - strand, within ABZR87_RS15510 at 3268.135 kb on - strand, within ABZR87_RS15510 at 3268.161 kb on + strand, within ABZR87_RS15510 at 3268.162 kb on - strand, within ABZR87_RS15510 at 3268.188 kb on + strand, within ABZR87_RS15510 at 3268.188 kb on + strand, within ABZR87_RS15510 at 3268.188 kb on + strand, within ABZR87_RS15510 at 3268.189 kb on - strand, within ABZR87_RS15510 at 3268.189 kb on - strand, within ABZR87_RS15510 at 3268.189 kb on - strand, within ABZR87_RS15510 at 3268.314 kb on + strand at 3268.314 kb on + strand at 3268.314 kb on + strand at 3268.314 kb on + strand at 3268.314 kb on + strand at 3268.315 kb on - strand at 3268.315 kb on - strand at 3268.315 kb on - strand at 3268.315 kb on - strand at 3268.315 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 3,262,884 + ABZR87_RS15500 0.17 +0.7 3,262,923 + ABZR87_RS15500 0.20 +0.3 3,262,924 - ABZR87_RS15500 0.21 +0.5 3,262,992 - ABZR87_RS15500 0.27 +2.0 3,263,299 + ABZR87_RS15500 0.56 +0.1 3,263,300 - ABZR87_RS15500 0.56 +0.9 3,263,321 + ABZR87_RS15500 0.58 +2.1 3,263,322 - ABZR87_RS15500 0.58 +0.8 3,263,324 + ABZR87_RS15500 0.58 +0.6 3,263,384 + ABZR87_RS15500 0.64 +0.9 3,263,385 - ABZR87_RS15500 0.64 +2.0 3,263,385 - ABZR87_RS15500 0.64 +0.7 3,263,385 - ABZR87_RS15500 0.64 +0.9 3,263,453 + ABZR87_RS15500 0.70 +1.4 3,263,466 + ABZR87_RS15500 0.71 +3.1 3,263,478 + ABZR87_RS15500 0.72 +1.9 3,263,478 + ABZR87_RS15500 0.72 +0.1 3,263,478 + ABZR87_RS15500 0.72 +1.0 3,263,478 + ABZR87_RS15500 0.72 +1.3 3,263,478 + ABZR87_RS15500 0.72 +3.2 3,263,479 - ABZR87_RS15500 0.72 -0.4 3,263,546 + ABZR87_RS15500 0.79 +0.5 3,263,739 - +0.6 3,263,781 + +1.7 3,263,781 + +1.2 3,263,781 + +1.2 3,263,782 - +0.4 3,263,787 + +0.7 3,263,787 + +1.4 3,263,793 + +0.9 3,263,794 - -1.3 3,263,857 + +1.5 3,263,858 - +0.3 3,263,858 - +0.7 3,263,858 - +0.2 3,263,858 - -0.9 3,263,863 + +0.2 3,263,863 + +0.7 3,263,864 - -1.2 3,263,864 - -1.0 3,263,864 - -1.0 3,264,363 - ABZR87_RS15505 0.14 -1.0 3,267,386 - +0.1 3,267,464 + -0.3 3,267,467 - -0.4 3,267,478 - -0.4 3,267,539 + +0.8 3,267,540 - -1.0 3,267,540 - -0.2 3,267,549 + +0.6 3,267,550 - +0.1 3,267,550 - -0.9 3,267,945 + ABZR87_RS15510 0.49 +0.2 3,267,945 + ABZR87_RS15510 0.49 +0.2 3,267,945 + ABZR87_RS15510 0.49 +0.1 3,267,946 - ABZR87_RS15510 0.50 -1.3 3,267,946 - ABZR87_RS15510 0.50 -0.0 3,267,946 - ABZR87_RS15510 0.50 -0.3 3,267,984 + ABZR87_RS15510 0.54 -0.5 3,267,985 - ABZR87_RS15510 0.54 -0.2 3,267,985 - ABZR87_RS15510 0.54 +0.6 3,267,985 - ABZR87_RS15510 0.54 +0.7 3,267,985 - ABZR87_RS15510 0.54 +0.0 3,267,985 - ABZR87_RS15510 0.54 -0.0 3,267,985 - ABZR87_RS15510 0.54 -0.6 3,268,135 - ABZR87_RS15510 0.72 -1.3 3,268,135 - ABZR87_RS15510 0.72 +0.2 3,268,161 + ABZR87_RS15510 0.75 +0.9 3,268,162 - ABZR87_RS15510 0.75 -0.4 3,268,188 + ABZR87_RS15510 0.78 -0.6 3,268,188 + ABZR87_RS15510 0.78 +0.4 3,268,188 + ABZR87_RS15510 0.78 +0.0 3,268,189 - ABZR87_RS15510 0.78 -0.4 3,268,189 - ABZR87_RS15510 0.78 +0.7 3,268,189 - ABZR87_RS15510 0.78 -2.3 3,268,314 + -0.4 3,268,314 + -0.8 3,268,314 + -0.8 3,268,314 + +0.7 3,268,314 + +0.8 3,268,315 - -1.3 3,268,315 - +0.6 3,268,315 - +0.4 3,268,315 - -0.5 3,268,315 - +1.1
Or see this region's nucleotide sequence