Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS14985 and ABZR87_RS14990 are separated by 86 nucleotides ABZR87_RS14990 and ABZR87_RS14995 are separated by 12 nucleotides ABZR87_RS14995 and ABZR87_RS15000 are separated by 137 nucleotides
ABZR87_RS14985: ABZR87_RS14985 - UDP-2,3-diacylglucosamine diphosphatase, at 3,160,552 to 3,161,502
_RS14985
ABZR87_RS14990: ABZR87_RS14990 - glycosyltransferase family 1 protein, at 3,161,589 to 3,162,632
_RS14990
ABZR87_RS14995: ABZR87_RS14995 - diacylglycerol kinase, at 3,162,645 to 3,163,109
_RS14995
ABZR87_RS15000: ABZR87_RS15000 - TetR/AcrR family transcriptional regulator, at 3,163,247 to 3,163,966
_RS15000
Position (kb)
3161
3162
3163 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3160.780 kb on + strand, within ABZR87_RS14985 at 3160.780 kb on + strand, within ABZR87_RS14985 at 3160.780 kb on + strand, within ABZR87_RS14985 at 3160.822 kb on + strand, within ABZR87_RS14985 at 3160.822 kb on + strand, within ABZR87_RS14985 at 3160.822 kb on + strand, within ABZR87_RS14985 at 3160.822 kb on + strand, within ABZR87_RS14985 at 3160.822 kb on + strand, within ABZR87_RS14985 at 3160.823 kb on - strand, within ABZR87_RS14985 at 3160.823 kb on - strand, within ABZR87_RS14985 at 3160.871 kb on - strand, within ABZR87_RS14985 at 3161.111 kb on - strand, within ABZR87_RS14985 at 3161.128 kb on + strand, within ABZR87_RS14985 at 3161.128 kb on + strand, within ABZR87_RS14985 at 3161.128 kb on + strand, within ABZR87_RS14985 at 3161.210 kb on - strand, within ABZR87_RS14985 at 3161.210 kb on - strand, within ABZR87_RS14985 at 3161.210 kb on - strand, within ABZR87_RS14985 at 3161.316 kb on + strand, within ABZR87_RS14985 at 3161.316 kb on + strand, within ABZR87_RS14985 at 3161.317 kb on - strand, within ABZR87_RS14985 at 3161.357 kb on - strand, within ABZR87_RS14985 at 3161.513 kb on + strand at 3161.513 kb on + strand at 3161.513 kb on + strand at 3161.514 kb on - strand at 3161.514 kb on - strand at 3161.730 kb on + strand, within ABZR87_RS14990 at 3161.731 kb on - strand, within ABZR87_RS14990 at 3161.851 kb on - strand, within ABZR87_RS14990 at 3161.875 kb on - strand, within ABZR87_RS14990 at 3161.886 kb on + strand, within ABZR87_RS14990 at 3161.908 kb on - strand, within ABZR87_RS14990 at 3161.947 kb on - strand, within ABZR87_RS14990 at 3162.129 kb on + strand, within ABZR87_RS14990 at 3162.130 kb on - strand, within ABZR87_RS14990 at 3162.130 kb on - strand, within ABZR87_RS14990 at 3162.130 kb on - strand, within ABZR87_RS14990 at 3162.239 kb on + strand, within ABZR87_RS14990 at 3162.239 kb on + strand, within ABZR87_RS14990 at 3162.239 kb on + strand, within ABZR87_RS14990 at 3162.239 kb on + strand, within ABZR87_RS14990 at 3162.240 kb on - strand, within ABZR87_RS14990 at 3162.240 kb on - strand, within ABZR87_RS14990 at 3162.240 kb on - strand, within ABZR87_RS14990 at 3162.240 kb on - strand, within ABZR87_RS14990 at 3162.240 kb on - strand, within ABZR87_RS14990 at 3162.240 kb on - strand, within ABZR87_RS14990 at 3162.243 kb on + strand, within ABZR87_RS14990 at 3162.244 kb on - strand, within ABZR87_RS14990 at 3162.259 kb on - strand, within ABZR87_RS14990 at 3162.297 kb on + strand, within ABZR87_RS14990 at 3162.297 kb on + strand, within ABZR87_RS14990 at 3162.297 kb on + strand, within ABZR87_RS14990 at 3162.298 kb on - strand, within ABZR87_RS14990 at 3162.298 kb on - strand, within ABZR87_RS14990 at 3162.353 kb on - strand, within ABZR87_RS14990 at 3162.418 kb on + strand, within ABZR87_RS14990 at 3162.419 kb on - strand, within ABZR87_RS14990 at 3162.485 kb on + strand, within ABZR87_RS14990 at 3162.485 kb on + strand, within ABZR87_RS14990 at 3162.485 kb on + strand, within ABZR87_RS14990 at 3162.485 kb on + strand, within ABZR87_RS14990 at 3162.734 kb on + strand, within ABZR87_RS14995 at 3162.734 kb on + strand, within ABZR87_RS14995 at 3162.735 kb on - strand, within ABZR87_RS14995 at 3163.107 kb on + strand at 3163.108 kb on - strand at 3163.108 kb on - strand at 3163.193 kb on - strand at 3163.195 kb on - strand at 3163.209 kb on + strand at 3163.210 kb on - strand at 3163.210 kb on - strand at 3163.210 kb on - strand at 3163.210 kb on - strand at 3163.210 kb on - strand at 3163.395 kb on - strand, within ABZR87_RS15000 at 3163.517 kb on + strand, within ABZR87_RS15000 at 3163.526 kb on + strand, within ABZR87_RS15000 at 3163.526 kb on + strand, within ABZR87_RS15000 at 3163.526 kb on + strand, within ABZR87_RS15000 at 3163.526 kb on + strand, within ABZR87_RS15000 at 3163.527 kb on - strand, within ABZR87_RS15000 at 3163.527 kb on - strand, within ABZR87_RS15000 at 3163.527 kb on - strand, within ABZR87_RS15000 at 3163.527 kb on - strand, within ABZR87_RS15000 at 3163.527 kb on - strand, within ABZR87_RS15000 at 3163.550 kb on + strand, within ABZR87_RS15000 at 3163.550 kb on + strand, within ABZR87_RS15000 at 3163.550 kb on + strand, within ABZR87_RS15000 at 3163.551 kb on - strand, within ABZR87_RS15000
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 3,160,780 + ABZR87_RS14985 0.24 +0.6 3,160,780 + ABZR87_RS14985 0.24 -0.8 3,160,780 + ABZR87_RS14985 0.24 -0.1 3,160,822 + ABZR87_RS14985 0.28 -0.2 3,160,822 + ABZR87_RS14985 0.28 +0.4 3,160,822 + ABZR87_RS14985 0.28 -0.4 3,160,822 + ABZR87_RS14985 0.28 +0.0 3,160,822 + ABZR87_RS14985 0.28 +0.1 3,160,823 - ABZR87_RS14985 0.28 -3.3 3,160,823 - ABZR87_RS14985 0.28 -1.0 3,160,871 - ABZR87_RS14985 0.34 +0.3 3,161,111 - ABZR87_RS14985 0.59 +0.1 3,161,128 + ABZR87_RS14985 0.61 +0.7 3,161,128 + ABZR87_RS14985 0.61 -0.5 3,161,128 + ABZR87_RS14985 0.61 -1.0 3,161,210 - ABZR87_RS14985 0.69 -0.0 3,161,210 - ABZR87_RS14985 0.69 -0.7 3,161,210 - ABZR87_RS14985 0.69 -0.6 3,161,316 + ABZR87_RS14985 0.80 -0.2 3,161,316 + ABZR87_RS14985 0.80 -0.2 3,161,317 - ABZR87_RS14985 0.80 +0.8 3,161,357 - ABZR87_RS14985 0.85 +0.6 3,161,513 + +0.7 3,161,513 + +0.1 3,161,513 + +1.0 3,161,514 - -0.1 3,161,514 - +0.7 3,161,730 + ABZR87_RS14990 0.14 +0.3 3,161,731 - ABZR87_RS14990 0.14 -0.2 3,161,851 - ABZR87_RS14990 0.25 -0.5 3,161,875 - ABZR87_RS14990 0.27 -0.5 3,161,886 + ABZR87_RS14990 0.28 +0.0 3,161,908 - ABZR87_RS14990 0.31 +0.4 3,161,947 - ABZR87_RS14990 0.34 +0.1 3,162,129 + ABZR87_RS14990 0.52 -1.5 3,162,130 - ABZR87_RS14990 0.52 -0.2 3,162,130 - ABZR87_RS14990 0.52 +0.1 3,162,130 - ABZR87_RS14990 0.52 +0.3 3,162,239 + ABZR87_RS14990 0.62 -0.6 3,162,239 + ABZR87_RS14990 0.62 +1.1 3,162,239 + ABZR87_RS14990 0.62 -0.5 3,162,239 + ABZR87_RS14990 0.62 +1.0 3,162,240 - ABZR87_RS14990 0.62 +0.6 3,162,240 - ABZR87_RS14990 0.62 +0.2 3,162,240 - ABZR87_RS14990 0.62 -0.5 3,162,240 - ABZR87_RS14990 0.62 -0.2 3,162,240 - ABZR87_RS14990 0.62 -0.0 3,162,240 - ABZR87_RS14990 0.62 -0.5 3,162,243 + ABZR87_RS14990 0.63 -0.7 3,162,244 - ABZR87_RS14990 0.63 -1.0 3,162,259 - ABZR87_RS14990 0.64 +0.1 3,162,297 + ABZR87_RS14990 0.68 -0.6 3,162,297 + ABZR87_RS14990 0.68 +0.2 3,162,297 + ABZR87_RS14990 0.68 -0.4 3,162,298 - ABZR87_RS14990 0.68 +0.7 3,162,298 - ABZR87_RS14990 0.68 +0.7 3,162,353 - ABZR87_RS14990 0.73 +0.1 3,162,418 + ABZR87_RS14990 0.79 -0.0 3,162,419 - ABZR87_RS14990 0.80 +0.7 3,162,485 + ABZR87_RS14990 0.86 -0.7 3,162,485 + ABZR87_RS14990 0.86 +0.8 3,162,485 + ABZR87_RS14990 0.86 +0.3 3,162,485 + ABZR87_RS14990 0.86 +0.3 3,162,734 + ABZR87_RS14995 0.19 -0.3 3,162,734 + ABZR87_RS14995 0.19 +0.8 3,162,735 - ABZR87_RS14995 0.19 +0.2 3,163,107 + -0.4 3,163,108 - -0.9 3,163,108 - -0.5 3,163,193 - -0.3 3,163,195 - +0.3 3,163,209 + -0.8 3,163,210 - -1.7 3,163,210 - -0.7 3,163,210 - +0.5 3,163,210 - +0.1 3,163,210 - +0.3 3,163,395 - ABZR87_RS15000 0.21 +0.7 3,163,517 + ABZR87_RS15000 0.38 +0.2 3,163,526 + ABZR87_RS15000 0.39 -0.5 3,163,526 + ABZR87_RS15000 0.39 -1.7 3,163,526 + ABZR87_RS15000 0.39 -0.4 3,163,526 + ABZR87_RS15000 0.39 -0.4 3,163,527 - ABZR87_RS15000 0.39 +0.4 3,163,527 - ABZR87_RS15000 0.39 -0.2 3,163,527 - ABZR87_RS15000 0.39 +0.6 3,163,527 - ABZR87_RS15000 0.39 +0.1 3,163,527 - ABZR87_RS15000 0.39 -0.7 3,163,550 + ABZR87_RS15000 0.42 +0.7 3,163,550 + ABZR87_RS15000 0.42 -0.4 3,163,550 + ABZR87_RS15000 0.42 +0.2 3,163,551 - ABZR87_RS15000 0.42 -0.1
Or see this region's nucleotide sequence