Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS14970 and ABZR87_RS14975 are separated by 96 nucleotides ABZR87_RS14975 and ABZR87_RS14980 are separated by 30 nucleotides ABZR87_RS14980 and ABZR87_RS14985 are separated by 244 nucleotides
ABZR87_RS14970: ABZR87_RS14970 - DUF3106 domain-containing protein, at 3,157,794 to 3,158,639
_RS14970
ABZR87_RS14975: ABZR87_RS14975 - RDD family protein, at 3,158,736 to 3,159,260
_RS14975
ABZR87_RS14980: ABZR87_RS14980 - alpha/beta fold hydrolase, at 3,159,291 to 3,160,307
_RS14980
ABZR87_RS14985: ABZR87_RS14985 - UDP-2,3-diacylglucosamine diphosphatase, at 3,160,552 to 3,161,502
_RS14985
Position (kb)
3159
3160
3161 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3158.426 kb on + strand, within ABZR87_RS14970 at 3158.426 kb on + strand, within ABZR87_RS14970 at 3158.661 kb on - strand at 3158.826 kb on + strand, within ABZR87_RS14975 at 3158.826 kb on + strand, within ABZR87_RS14975 at 3158.827 kb on - strand, within ABZR87_RS14975 at 3158.827 kb on - strand, within ABZR87_RS14975 at 3158.827 kb on - strand, within ABZR87_RS14975 at 3158.935 kb on + strand, within ABZR87_RS14975 at 3158.935 kb on + strand, within ABZR87_RS14975 at 3158.936 kb on - strand, within ABZR87_RS14975 at 3158.936 kb on - strand, within ABZR87_RS14975 at 3158.961 kb on + strand, within ABZR87_RS14975 at 3158.961 kb on + strand, within ABZR87_RS14975 at 3158.962 kb on - strand, within ABZR87_RS14975 at 3158.962 kb on - strand, within ABZR87_RS14975 at 3158.962 kb on - strand, within ABZR87_RS14975 at 3158.962 kb on - strand, within ABZR87_RS14975 at 3159.047 kb on + strand, within ABZR87_RS14975 at 3159.076 kb on - strand, within ABZR87_RS14975 at 3159.115 kb on + strand, within ABZR87_RS14975 at 3159.116 kb on - strand, within ABZR87_RS14975 at 3159.116 kb on - strand, within ABZR87_RS14975 at 3159.735 kb on + strand, within ABZR87_RS14980 at 3159.735 kb on + strand, within ABZR87_RS14980 at 3159.736 kb on - strand, within ABZR87_RS14980 at 3159.843 kb on + strand, within ABZR87_RS14980 at 3159.880 kb on + strand, within ABZR87_RS14980 at 3159.881 kb on - strand, within ABZR87_RS14980 at 3160.228 kb on + strand at 3160.229 kb on - strand at 3160.229 kb on - strand at 3160.780 kb on + strand, within ABZR87_RS14985 at 3160.780 kb on + strand, within ABZR87_RS14985 at 3160.780 kb on + strand, within ABZR87_RS14985 at 3160.822 kb on + strand, within ABZR87_RS14985 at 3160.822 kb on + strand, within ABZR87_RS14985 at 3160.822 kb on + strand, within ABZR87_RS14985 at 3160.822 kb on + strand, within ABZR87_RS14985 at 3160.822 kb on + strand, within ABZR87_RS14985 at 3160.823 kb on - strand, within ABZR87_RS14985 at 3160.823 kb on - strand, within ABZR87_RS14985 at 3160.871 kb on - strand, within ABZR87_RS14985 at 3161.111 kb on - strand, within ABZR87_RS14985 at 3161.128 kb on + strand, within ABZR87_RS14985 at 3161.128 kb on + strand, within ABZR87_RS14985 at 3161.128 kb on + strand, within ABZR87_RS14985 at 3161.210 kb on - strand, within ABZR87_RS14985 at 3161.210 kb on - strand, within ABZR87_RS14985 at 3161.210 kb on - strand, within ABZR87_RS14985
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 3,158,426 + ABZR87_RS14970 0.75 +0.2 3,158,426 + ABZR87_RS14970 0.75 -0.7 3,158,661 - +0.7 3,158,826 + ABZR87_RS14975 0.17 -1.5 3,158,826 + ABZR87_RS14975 0.17 -0.0 3,158,827 - ABZR87_RS14975 0.17 -1.2 3,158,827 - ABZR87_RS14975 0.17 -0.2 3,158,827 - ABZR87_RS14975 0.17 +1.1 3,158,935 + ABZR87_RS14975 0.38 +0.3 3,158,935 + ABZR87_RS14975 0.38 +0.4 3,158,936 - ABZR87_RS14975 0.38 -0.9 3,158,936 - ABZR87_RS14975 0.38 -1.0 3,158,961 + ABZR87_RS14975 0.43 -2.1 3,158,961 + ABZR87_RS14975 0.43 +1.1 3,158,962 - ABZR87_RS14975 0.43 -0.2 3,158,962 - ABZR87_RS14975 0.43 +0.5 3,158,962 - ABZR87_RS14975 0.43 -0.1 3,158,962 - ABZR87_RS14975 0.43 -0.3 3,159,047 + ABZR87_RS14975 0.59 -0.6 3,159,076 - ABZR87_RS14975 0.65 -0.1 3,159,115 + ABZR87_RS14975 0.72 +0.1 3,159,116 - ABZR87_RS14975 0.72 +0.1 3,159,116 - ABZR87_RS14975 0.72 +0.8 3,159,735 + ABZR87_RS14980 0.44 -1.3 3,159,735 + ABZR87_RS14980 0.44 -0.7 3,159,736 - ABZR87_RS14980 0.44 -0.2 3,159,843 + ABZR87_RS14980 0.54 +0.0 3,159,880 + ABZR87_RS14980 0.58 -1.0 3,159,881 - ABZR87_RS14980 0.58 -1.7 3,160,228 + -0.6 3,160,229 - +0.2 3,160,229 - -0.6 3,160,780 + ABZR87_RS14985 0.24 +0.6 3,160,780 + ABZR87_RS14985 0.24 -0.8 3,160,780 + ABZR87_RS14985 0.24 -0.1 3,160,822 + ABZR87_RS14985 0.28 -0.2 3,160,822 + ABZR87_RS14985 0.28 +0.4 3,160,822 + ABZR87_RS14985 0.28 -0.4 3,160,822 + ABZR87_RS14985 0.28 +0.0 3,160,822 + ABZR87_RS14985 0.28 +0.1 3,160,823 - ABZR87_RS14985 0.28 -3.3 3,160,823 - ABZR87_RS14985 0.28 -1.0 3,160,871 - ABZR87_RS14985 0.34 +0.3 3,161,111 - ABZR87_RS14985 0.59 +0.1 3,161,128 + ABZR87_RS14985 0.61 +0.7 3,161,128 + ABZR87_RS14985 0.61 -0.5 3,161,128 + ABZR87_RS14985 0.61 -1.0 3,161,210 - ABZR87_RS14985 0.69 -0.0 3,161,210 - ABZR87_RS14985 0.69 -0.7 3,161,210 - ABZR87_RS14985 0.69 -0.6
Or see this region's nucleotide sequence