Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS14530 and ABZR87_RS14535 are separated by 29 nucleotides ABZR87_RS14535 and ABZR87_RS14540 are separated by 23 nucleotides ABZR87_RS14540 and ABZR87_RS14545 are separated by 227 nucleotides
ABZR87_RS14530: ABZR87_RS14530 - 3-isopropylmalate dehydrogenase, at 3,069,721 to 3,070,791
_RS14530
ABZR87_RS14535: ABZR87_RS14535 - 3-isopropylmalate dehydratase small subunit, at 3,070,821 to 3,071,471
_RS14535
ABZR87_RS14540: ABZR87_RS14540 - 3-isopropylmalate dehydratase large subunit, at 3,071,495 to 3,072,904
_RS14540
ABZR87_RS14545: ABZR87_RS14545 - citrate synthase, at 3,073,132 to 3,074,433
_RS14545
Position (kb)
3071
3072
3073 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3070.571 kb on + strand, within ABZR87_RS14530 at 3070.571 kb on + strand, within ABZR87_RS14530 at 3070.876 kb on + strand at 3070.876 kb on + strand at 3070.876 kb on + strand at 3070.876 kb on + strand at 3070.877 kb on - strand at 3070.877 kb on - strand at 3070.877 kb on - strand at 3070.897 kb on + strand, within ABZR87_RS14535 at 3070.898 kb on - strand, within ABZR87_RS14535 at 3070.942 kb on + strand, within ABZR87_RS14535 at 3070.943 kb on - strand, within ABZR87_RS14535 at 3071.036 kb on + strand, within ABZR87_RS14535 at 3071.037 kb on - strand, within ABZR87_RS14535 at 3071.167 kb on + strand, within ABZR87_RS14535 at 3071.167 kb on + strand, within ABZR87_RS14535 at 3071.168 kb on - strand, within ABZR87_RS14535 at 3071.168 kb on - strand, within ABZR87_RS14535 at 3071.329 kb on + strand, within ABZR87_RS14535 at 3071.330 kb on - strand, within ABZR87_RS14535 at 3071.373 kb on - strand, within ABZR87_RS14535 at 3071.484 kb on + strand at 3071.484 kb on + strand at 3071.484 kb on + strand at 3071.485 kb on - strand at 3071.485 kb on - strand at 3071.562 kb on - strand at 3071.562 kb on - strand at 3071.562 kb on - strand at 3071.931 kb on + strand, within ABZR87_RS14540 at 3071.931 kb on + strand, within ABZR87_RS14540 at 3071.931 kb on + strand, within ABZR87_RS14540 at 3071.931 kb on + strand, within ABZR87_RS14540 at 3071.931 kb on + strand, within ABZR87_RS14540 at 3071.931 kb on + strand, within ABZR87_RS14540 at 3071.932 kb on - strand, within ABZR87_RS14540 at 3072.086 kb on + strand, within ABZR87_RS14540 at 3072.180 kb on + strand, within ABZR87_RS14540 at 3072.181 kb on - strand, within ABZR87_RS14540 at 3072.449 kb on + strand, within ABZR87_RS14540 at 3072.449 kb on + strand, within ABZR87_RS14540 at 3072.450 kb on - strand, within ABZR87_RS14540 at 3072.628 kb on - strand, within ABZR87_RS14540 at 3072.628 kb on - strand, within ABZR87_RS14540 at 3072.828 kb on + strand at 3072.828 kb on + strand at 3072.828 kb on + strand at 3072.828 kb on + strand at 3072.829 kb on - strand at 3072.829 kb on - strand at 3072.829 kb on - strand at 3072.856 kb on + strand at 3072.916 kb on + strand at 3072.982 kb on + strand at 3072.982 kb on + strand at 3073.133 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 3,070,571 + ABZR87_RS14530 0.79 -1.1 3,070,571 + ABZR87_RS14530 0.79 -0.0 3,070,876 + -1.6 3,070,876 + -1.3 3,070,876 + -0.8 3,070,876 + +0.2 3,070,877 - -2.8 3,070,877 - -0.2 3,070,877 - +0.2 3,070,897 + ABZR87_RS14535 0.12 +0.4 3,070,898 - ABZR87_RS14535 0.12 -0.4 3,070,942 + ABZR87_RS14535 0.19 +0.5 3,070,943 - ABZR87_RS14535 0.19 -1.8 3,071,036 + ABZR87_RS14535 0.33 -0.1 3,071,037 - ABZR87_RS14535 0.33 -0.1 3,071,167 + ABZR87_RS14535 0.53 -0.4 3,071,167 + ABZR87_RS14535 0.53 -1.9 3,071,168 - ABZR87_RS14535 0.53 -1.0 3,071,168 - ABZR87_RS14535 0.53 +1.1 3,071,329 + ABZR87_RS14535 0.78 +0.7 3,071,330 - ABZR87_RS14535 0.78 +1.0 3,071,373 - ABZR87_RS14535 0.85 -0.1 3,071,484 + +0.4 3,071,484 + -0.5 3,071,484 + +0.9 3,071,485 - -0.4 3,071,485 - +0.0 3,071,562 - -0.7 3,071,562 - -1.8 3,071,562 - +0.2 3,071,931 + ABZR87_RS14540 0.31 -0.5 3,071,931 + ABZR87_RS14540 0.31 -0.3 3,071,931 + ABZR87_RS14540 0.31 -2.8 3,071,931 + ABZR87_RS14540 0.31 +1.4 3,071,931 + ABZR87_RS14540 0.31 -1.8 3,071,931 + ABZR87_RS14540 0.31 -0.9 3,071,932 - ABZR87_RS14540 0.31 -0.3 3,072,086 + ABZR87_RS14540 0.42 -0.3 3,072,180 + ABZR87_RS14540 0.49 -1.4 3,072,181 - ABZR87_RS14540 0.49 -2.1 3,072,449 + ABZR87_RS14540 0.68 -1.7 3,072,449 + ABZR87_RS14540 0.68 -1.5 3,072,450 - ABZR87_RS14540 0.68 -0.6 3,072,628 - ABZR87_RS14540 0.80 +0.1 3,072,628 - ABZR87_RS14540 0.80 +0.5 3,072,828 + +0.8 3,072,828 + +2.0 3,072,828 + -0.7 3,072,828 + +1.7 3,072,829 - -1.6 3,072,829 - +0.5 3,072,829 - -0.0 3,072,856 + -0.6 3,072,916 + -0.8 3,072,982 + +0.1 3,072,982 + -1.7 3,073,133 + +1.0
Or see this region's nucleotide sequence