Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS14160 and ABZR87_RS14165 are separated by 138 nucleotides ABZR87_RS14165 and ABZR87_RS14170 overlap by 2 nucleotides ABZR87_RS14170 and ABZR87_RS14175 are separated by 39 nucleotides ABZR87_RS14175 and ABZR87_RS14180 are separated by 17 nucleotides
ABZR87_RS14160: ABZR87_RS14160 - transglycosylase SLT domain-containing protein, at 2,991,690 to 2,993,240
_RS14160
ABZR87_RS14165: ABZR87_RS14165 - hydroxyacylglutathione hydrolase, at 2,993,379 to 2,994,218
_RS14165
ABZR87_RS14170: ABZR87_RS14170 - class I SAM-dependent methyltransferase, at 2,994,217 to 2,995,122
_RS14170
ABZR87_RS14175: ABZR87_RS14175 - ribonuclease HI, at 2,995,162 to 2,995,608
_RS14175
ABZR87_RS14180: ABZR87_RS14180 - hypothetical protein, at 2,995,626 to 2,996,135
_RS14180
Position (kb)
2994
2995
2996 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2993.278 kb on + strand at 2993.278 kb on + strand at 2993.278 kb on + strand at 2993.279 kb on - strand at 2993.279 kb on - strand at 2993.279 kb on - strand at 2993.282 kb on - strand at 2993.282 kb on - strand at 2993.282 kb on - strand at 2993.418 kb on + strand at 2993.418 kb on + strand at 2993.419 kb on - strand at 2993.568 kb on + strand, within ABZR87_RS14165 at 2993.569 kb on - strand, within ABZR87_RS14165 at 2993.644 kb on + strand, within ABZR87_RS14165 at 2993.644 kb on + strand, within ABZR87_RS14165 at 2993.644 kb on + strand, within ABZR87_RS14165 at 2993.644 kb on + strand, within ABZR87_RS14165 at 2993.644 kb on + strand, within ABZR87_RS14165 at 2993.645 kb on - strand, within ABZR87_RS14165 at 2993.645 kb on - strand, within ABZR87_RS14165 at 2993.645 kb on - strand, within ABZR87_RS14165 at 2993.645 kb on - strand, within ABZR87_RS14165 at 2993.645 kb on - strand, within ABZR87_RS14165 at 2993.645 kb on - strand, within ABZR87_RS14165 at 2993.645 kb on - strand, within ABZR87_RS14165 at 2993.645 kb on - strand, within ABZR87_RS14165 at 2993.645 kb on - strand, within ABZR87_RS14165 at 2993.659 kb on + strand, within ABZR87_RS14165 at 2993.660 kb on - strand, within ABZR87_RS14165 at 2993.670 kb on - strand, within ABZR87_RS14165 at 2993.762 kb on + strand, within ABZR87_RS14165 at 2993.763 kb on - strand, within ABZR87_RS14165 at 2993.763 kb on - strand, within ABZR87_RS14165 at 2993.763 kb on - strand, within ABZR87_RS14165 at 2993.763 kb on - strand, within ABZR87_RS14165 at 2993.815 kb on + strand, within ABZR87_RS14165 at 2993.816 kb on - strand, within ABZR87_RS14165 at 2993.816 kb on - strand, within ABZR87_RS14165 at 2993.816 kb on - strand, within ABZR87_RS14165 at 2993.816 kb on - strand, within ABZR87_RS14165 at 2993.816 kb on - strand, within ABZR87_RS14165 at 2993.816 kb on - strand, within ABZR87_RS14165 at 2993.834 kb on + strand, within ABZR87_RS14165 at 2993.834 kb on + strand, within ABZR87_RS14165 at 2993.835 kb on - strand, within ABZR87_RS14165 at 2994.039 kb on + strand, within ABZR87_RS14165 at 2994.039 kb on + strand, within ABZR87_RS14165 at 2994.039 kb on + strand, within ABZR87_RS14165 at 2994.040 kb on - strand, within ABZR87_RS14165 at 2994.136 kb on - strand at 2994.146 kb on + strand at 2994.146 kb on + strand at 2994.146 kb on + strand at 2994.146 kb on + strand at 2994.146 kb on + strand at 2994.147 kb on - strand at 2994.147 kb on - strand at 2994.147 kb on - strand at 2994.148 kb on + strand at 2994.148 kb on + strand at 2994.148 kb on + strand at 2994.149 kb on - strand at 2994.149 kb on - strand at 2994.149 kb on - strand at 2994.190 kb on - strand at 2994.190 kb on - strand at 2994.190 kb on - strand at 2994.248 kb on + strand at 2994.248 kb on + strand at 2994.248 kb on + strand at 2994.248 kb on + strand at 2994.249 kb on - strand at 2994.249 kb on - strand at 2994.249 kb on - strand at 2994.249 kb on - strand at 2995.041 kb on + strand at 2995.041 kb on + strand at 2995.042 kb on - strand at 2995.042 kb on - strand at 2995.180 kb on + strand at 2995.688 kb on - strand, within ABZR87_RS14180 at 2995.688 kb on - strand, within ABZR87_RS14180 at 2995.711 kb on + strand, within ABZR87_RS14180 at 2995.711 kb on + strand, within ABZR87_RS14180 at 2995.867 kb on + strand, within ABZR87_RS14180 at 2995.868 kb on - strand, within ABZR87_RS14180 at 2995.888 kb on + strand, within ABZR87_RS14180 at 2995.894 kb on + strand, within ABZR87_RS14180 at 2995.894 kb on + strand, within ABZR87_RS14180 at 2995.895 kb on - strand, within ABZR87_RS14180 at 2995.895 kb on - strand, within ABZR87_RS14180 at 2996.032 kb on + strand, within ABZR87_RS14180 at 2996.032 kb on + strand, within ABZR87_RS14180 at 2996.033 kb on - strand, within ABZR87_RS14180 at 2996.033 kb on - strand, within ABZR87_RS14180 at 2996.083 kb on + strand, within ABZR87_RS14180 at 2996.083 kb on + strand, within ABZR87_RS14180 at 2996.084 kb on - strand, within ABZR87_RS14180
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,993,278 + -1.0 2,993,278 + -0.5 2,993,278 + -0.2 2,993,279 - +0.3 2,993,279 - -0.9 2,993,279 - -1.9 2,993,282 - +0.4 2,993,282 - +0.1 2,993,282 - +0.5 2,993,418 + +0.6 2,993,418 + +0.5 2,993,419 - -0.8 2,993,568 + ABZR87_RS14165 0.23 -1.4 2,993,569 - ABZR87_RS14165 0.23 -0.0 2,993,644 + ABZR87_RS14165 0.32 +0.1 2,993,644 + ABZR87_RS14165 0.32 -0.1 2,993,644 + ABZR87_RS14165 0.32 +0.5 2,993,644 + ABZR87_RS14165 0.32 +0.3 2,993,644 + ABZR87_RS14165 0.32 -0.4 2,993,645 - ABZR87_RS14165 0.32 +0.1 2,993,645 - ABZR87_RS14165 0.32 +0.0 2,993,645 - ABZR87_RS14165 0.32 -0.8 2,993,645 - ABZR87_RS14165 0.32 -1.2 2,993,645 - ABZR87_RS14165 0.32 -0.0 2,993,645 - ABZR87_RS14165 0.32 +0.2 2,993,645 - ABZR87_RS14165 0.32 +0.2 2,993,645 - ABZR87_RS14165 0.32 -0.0 2,993,645 - ABZR87_RS14165 0.32 +0.0 2,993,659 + ABZR87_RS14165 0.33 -0.6 2,993,660 - ABZR87_RS14165 0.33 -0.6 2,993,670 - ABZR87_RS14165 0.35 +0.3 2,993,762 + ABZR87_RS14165 0.46 -0.2 2,993,763 - ABZR87_RS14165 0.46 -0.9 2,993,763 - ABZR87_RS14165 0.46 -0.4 2,993,763 - ABZR87_RS14165 0.46 -0.2 2,993,763 - ABZR87_RS14165 0.46 -0.9 2,993,815 + ABZR87_RS14165 0.52 +1.4 2,993,816 - ABZR87_RS14165 0.52 +1.3 2,993,816 - ABZR87_RS14165 0.52 +0.5 2,993,816 - ABZR87_RS14165 0.52 +0.9 2,993,816 - ABZR87_RS14165 0.52 +1.0 2,993,816 - ABZR87_RS14165 0.52 +0.1 2,993,816 - ABZR87_RS14165 0.52 +0.8 2,993,834 + ABZR87_RS14165 0.54 +1.7 2,993,834 + ABZR87_RS14165 0.54 -0.3 2,993,835 - ABZR87_RS14165 0.54 +0.1 2,994,039 + ABZR87_RS14165 0.79 +0.4 2,994,039 + ABZR87_RS14165 0.79 +0.6 2,994,039 + ABZR87_RS14165 0.79 +0.6 2,994,040 - ABZR87_RS14165 0.79 +0.9 2,994,136 - -0.5 2,994,146 + +0.1 2,994,146 + +0.7 2,994,146 + +1.0 2,994,146 + +0.4 2,994,146 + +0.2 2,994,147 - -0.6 2,994,147 - +0.1 2,994,147 - +0.2 2,994,148 + +0.6 2,994,148 + +0.0 2,994,148 + +0.6 2,994,149 - -0.9 2,994,149 - -0.2 2,994,149 - +1.0 2,994,190 - +2.0 2,994,190 - -1.0 2,994,190 - -2.1 2,994,248 + -0.6 2,994,248 + -0.0 2,994,248 + +0.2 2,994,248 + +1.6 2,994,249 - -1.8 2,994,249 - -1.9 2,994,249 - -1.0 2,994,249 - -0.9 2,995,041 + -0.9 2,995,041 + -0.4 2,995,042 - -1.0 2,995,042 - +0.1 2,995,180 + +1.0 2,995,688 - ABZR87_RS14180 0.12 +0.4 2,995,688 - ABZR87_RS14180 0.12 +0.4 2,995,711 + ABZR87_RS14180 0.17 +0.6 2,995,711 + ABZR87_RS14180 0.17 +0.6 2,995,867 + ABZR87_RS14180 0.47 +0.7 2,995,868 - ABZR87_RS14180 0.47 +0.8 2,995,888 + ABZR87_RS14180 0.51 +0.8 2,995,894 + ABZR87_RS14180 0.53 +0.1 2,995,894 + ABZR87_RS14180 0.53 +0.5 2,995,895 - ABZR87_RS14180 0.53 +0.4 2,995,895 - ABZR87_RS14180 0.53 -0.3 2,996,032 + ABZR87_RS14180 0.80 +0.2 2,996,032 + ABZR87_RS14180 0.80 -0.9 2,996,033 - ABZR87_RS14180 0.80 -1.6 2,996,033 - ABZR87_RS14180 0.80 -0.1 2,996,083 + ABZR87_RS14180 0.90 -1.1 2,996,083 + ABZR87_RS14180 0.90 -1.3 2,996,084 - ABZR87_RS14180 0.90 +0.1
Or see this region's nucleotide sequence