Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS13380 and ABZR87_RS13385 overlap by 14 nucleotides ABZR87_RS13385 and ABZR87_RS13390 are separated by 0 nucleotides ABZR87_RS13390 and ABZR87_RS13395 are separated by 14 nucleotides ABZR87_RS13395 and ABZR87_RS13400 are separated by 69 nucleotides ABZR87_RS13400 and ABZR87_RS13405 overlap by 4 nucleotides
ABZR87_RS13380: ABZR87_RS13380 - leucyl/phenylalanyl-tRNA--protein transferase, at 2,835,922 to 2,836,668
_RS13380
ABZR87_RS13385: ABZR87_RS13385 - arginyltransferase, at 2,836,655 to 2,837,422
_RS13385
ABZR87_RS13390: ABZR87_RS13390 - hypothetical protein, at 2,837,423 to 2,837,590
_RS13390
ABZR87_RS13395: ABZR87_RS13395 - TM2 domain-containing protein, at 2,837,605 to 2,837,919
_RS13395
ABZR87_RS13400: ABZR87_RS13400 - hypothetical protein, at 2,837,989 to 2,838,270
_RS13400
ABZR87_RS13405: ABZR87_RS13405 - LutB/LldF family L-lactate oxidation iron-sulfur protein, at 2,838,267 to 2,839,676
_RS13405
Position (kb)
2837
2838 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2836.499 kb on + strand, within ABZR87_RS13380 at 2836.499 kb on + strand, within ABZR87_RS13380 at 2836.499 kb on + strand, within ABZR87_RS13380 at 2836.499 kb on + strand, within ABZR87_RS13380 at 2836.500 kb on - strand, within ABZR87_RS13380 at 2836.500 kb on - strand, within ABZR87_RS13380 at 2836.500 kb on - strand, within ABZR87_RS13380 at 2836.500 kb on - strand, within ABZR87_RS13380 at 2836.500 kb on - strand, within ABZR87_RS13380 at 2836.500 kb on - strand, within ABZR87_RS13380 at 2836.666 kb on + strand at 2836.666 kb on + strand at 2836.666 kb on + strand at 2836.666 kb on + strand at 2836.697 kb on + strand at 2836.697 kb on + strand at 2836.697 kb on + strand at 2836.697 kb on + strand at 2836.697 kb on + strand at 2836.697 kb on + strand at 2836.698 kb on - strand at 2836.698 kb on - strand at 2836.698 kb on - strand at 2836.698 kb on - strand at 2836.698 kb on - strand at 2836.698 kb on - strand at 2836.698 kb on - strand at 2836.698 kb on - strand at 2836.713 kb on - strand at 2836.713 kb on - strand at 2836.724 kb on + strand at 2836.724 kb on + strand at 2836.724 kb on + strand at 2836.724 kb on + strand at 2836.724 kb on + strand at 2836.724 kb on + strand at 2836.725 kb on - strand at 2836.725 kb on - strand at 2836.790 kb on + strand, within ABZR87_RS13385 at 2836.791 kb on - strand, within ABZR87_RS13385 at 2836.791 kb on - strand, within ABZR87_RS13385 at 2836.838 kb on + strand, within ABZR87_RS13385 at 2836.838 kb on + strand, within ABZR87_RS13385 at 2836.838 kb on + strand, within ABZR87_RS13385 at 2836.838 kb on + strand, within ABZR87_RS13385 at 2836.898 kb on + strand, within ABZR87_RS13385 at 2836.899 kb on - strand, within ABZR87_RS13385 at 2836.973 kb on + strand, within ABZR87_RS13385 at 2836.973 kb on + strand, within ABZR87_RS13385 at 2836.974 kb on - strand, within ABZR87_RS13385 at 2836.974 kb on - strand, within ABZR87_RS13385 at 2836.974 kb on - strand, within ABZR87_RS13385 at 2836.974 kb on - strand, within ABZR87_RS13385 at 2836.974 kb on - strand, within ABZR87_RS13385 at 2836.974 kb on - strand, within ABZR87_RS13385 at 2836.974 kb on - strand, within ABZR87_RS13385 at 2836.988 kb on + strand, within ABZR87_RS13385 at 2836.988 kb on + strand, within ABZR87_RS13385 at 2836.988 kb on + strand, within ABZR87_RS13385 at 2836.988 kb on + strand, within ABZR87_RS13385 at 2836.988 kb on + strand, within ABZR87_RS13385 at 2836.988 kb on + strand, within ABZR87_RS13385 at 2836.989 kb on - strand, within ABZR87_RS13385 at 2836.989 kb on - strand, within ABZR87_RS13385 at 2836.997 kb on + strand, within ABZR87_RS13385 at 2837.060 kb on + strand, within ABZR87_RS13385 at 2837.060 kb on + strand, within ABZR87_RS13385 at 2837.060 kb on + strand, within ABZR87_RS13385 at 2837.060 kb on + strand, within ABZR87_RS13385 at 2837.060 kb on + strand, within ABZR87_RS13385 at 2837.060 kb on + strand, within ABZR87_RS13385 at 2837.195 kb on + strand, within ABZR87_RS13385 at 2837.195 kb on + strand, within ABZR87_RS13385 at 2837.195 kb on + strand, within ABZR87_RS13385 at 2837.196 kb on - strand, within ABZR87_RS13385 at 2837.196 kb on - strand, within ABZR87_RS13385 at 2837.205 kb on - strand, within ABZR87_RS13385 at 2837.240 kb on + strand, within ABZR87_RS13385 at 2837.240 kb on + strand, within ABZR87_RS13385 at 2837.240 kb on + strand, within ABZR87_RS13385 at 2837.240 kb on + strand, within ABZR87_RS13385 at 2837.240 kb on + strand, within ABZR87_RS13385 at 2837.240 kb on + strand, within ABZR87_RS13385 at 2837.240 kb on + strand, within ABZR87_RS13385 at 2837.241 kb on - strand, within ABZR87_RS13385 at 2837.241 kb on - strand, within ABZR87_RS13385 at 2837.241 kb on - strand, within ABZR87_RS13385 at 2837.241 kb on - strand, within ABZR87_RS13385 at 2837.241 kb on - strand, within ABZR87_RS13385 at 2837.241 kb on - strand, within ABZR87_RS13385 at 2837.241 kb on - strand, within ABZR87_RS13385 at 2837.288 kb on + strand, within ABZR87_RS13385 at 2837.288 kb on + strand, within ABZR87_RS13385 at 2837.288 kb on + strand, within ABZR87_RS13385 at 2837.288 kb on + strand, within ABZR87_RS13385 at 2837.289 kb on - strand, within ABZR87_RS13385 at 2837.289 kb on - strand, within ABZR87_RS13385 at 2837.289 kb on - strand, within ABZR87_RS13385 at 2837.289 kb on - strand, within ABZR87_RS13385 at 2837.289 kb on - strand, within ABZR87_RS13385 at 2837.294 kb on + strand, within ABZR87_RS13385 at 2837.295 kb on - strand, within ABZR87_RS13385 at 2837.295 kb on - strand, within ABZR87_RS13385 at 2837.333 kb on + strand, within ABZR87_RS13385 at 2837.333 kb on + strand, within ABZR87_RS13385 at 2837.333 kb on + strand, within ABZR87_RS13385 at 2837.333 kb on + strand, within ABZR87_RS13385 at 2837.333 kb on + strand, within ABZR87_RS13385 at 2837.334 kb on - strand, within ABZR87_RS13385 at 2837.463 kb on + strand, within ABZR87_RS13390 at 2837.463 kb on + strand, within ABZR87_RS13390 at 2837.463 kb on + strand, within ABZR87_RS13390 at 2837.464 kb on - strand, within ABZR87_RS13390 at 2837.490 kb on + strand, within ABZR87_RS13390 at 2837.490 kb on + strand, within ABZR87_RS13390 at 2837.730 kb on + strand, within ABZR87_RS13395 at 2837.731 kb on - strand, within ABZR87_RS13395 at 2837.731 kb on - strand, within ABZR87_RS13395 at 2837.757 kb on + strand, within ABZR87_RS13395 at 2837.758 kb on - strand, within ABZR87_RS13395 at 2837.758 kb on - strand, within ABZR87_RS13395 at 2837.810 kb on + strand, within ABZR87_RS13395 at 2837.810 kb on + strand, within ABZR87_RS13395 at 2837.811 kb on - strand, within ABZR87_RS13395 at 2837.855 kb on + strand, within ABZR87_RS13395 at 2837.855 kb on + strand, within ABZR87_RS13395 at 2837.855 kb on + strand, within ABZR87_RS13395 at 2837.855 kb on + strand, within ABZR87_RS13395 at 2837.856 kb on - strand, within ABZR87_RS13395 at 2837.856 kb on - strand, within ABZR87_RS13395 at 2837.888 kb on + strand at 2837.940 kb on + strand at 2837.941 kb on - strand at 2837.955 kb on + strand at 2837.956 kb on - strand at 2837.956 kb on - strand at 2837.956 kb on - strand at 2837.990 kb on + strand at 2837.990 kb on + strand at 2837.991 kb on - strand at 2837.991 kb on - strand at 2838.019 kb on - strand, within ABZR87_RS13400 at 2838.292 kb on + strand at 2838.292 kb on + strand at 2838.292 kb on + strand at 2838.292 kb on + strand at 2838.338 kb on + strand at 2838.338 kb on + strand at 2838.339 kb on - strand at 2838.425 kb on + strand, within ABZR87_RS13405 at 2838.426 kb on - strand, within ABZR87_RS13405 at 2838.436 kb on + strand, within ABZR87_RS13405 at 2838.533 kb on + strand, within ABZR87_RS13405 at 2838.533 kb on + strand, within ABZR87_RS13405 at 2838.533 kb on + strand, within ABZR87_RS13405
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,836,499 + ABZR87_RS13380 0.77 +0.5 2,836,499 + ABZR87_RS13380 0.77 +0.6 2,836,499 + ABZR87_RS13380 0.77 +0.3 2,836,499 + ABZR87_RS13380 0.77 +2.0 2,836,500 - ABZR87_RS13380 0.77 +0.3 2,836,500 - ABZR87_RS13380 0.77 +1.1 2,836,500 - ABZR87_RS13380 0.77 -0.1 2,836,500 - ABZR87_RS13380 0.77 +1.0 2,836,500 - ABZR87_RS13380 0.77 -1.5 2,836,500 - ABZR87_RS13380 0.77 -1.1 2,836,666 + +0.2 2,836,666 + -0.9 2,836,666 + +0.4 2,836,666 + -0.8 2,836,697 + +0.0 2,836,697 + +0.2 2,836,697 + +0.1 2,836,697 + +1.0 2,836,697 + -0.6 2,836,697 + -0.3 2,836,698 - -0.4 2,836,698 - -0.0 2,836,698 - -0.0 2,836,698 - -0.1 2,836,698 - +1.3 2,836,698 - -0.1 2,836,698 - -1.7 2,836,698 - -0.2 2,836,713 - -0.4 2,836,713 - -0.0 2,836,724 + +0.2 2,836,724 + +0.4 2,836,724 + +0.3 2,836,724 + +1.4 2,836,724 + +0.0 2,836,724 + -1.0 2,836,725 - +0.4 2,836,725 - -0.3 2,836,790 + ABZR87_RS13385 0.18 +0.6 2,836,791 - ABZR87_RS13385 0.18 -0.4 2,836,791 - ABZR87_RS13385 0.18 +0.1 2,836,838 + ABZR87_RS13385 0.24 -0.1 2,836,838 + ABZR87_RS13385 0.24 +0.0 2,836,838 + ABZR87_RS13385 0.24 +0.3 2,836,838 + ABZR87_RS13385 0.24 -0.1 2,836,898 + ABZR87_RS13385 0.32 -1.0 2,836,899 - ABZR87_RS13385 0.32 -0.1 2,836,973 + ABZR87_RS13385 0.41 +0.1 2,836,973 + ABZR87_RS13385 0.41 -1.3 2,836,974 - ABZR87_RS13385 0.42 -0.9 2,836,974 - ABZR87_RS13385 0.42 -0.1 2,836,974 - ABZR87_RS13385 0.42 +0.0 2,836,974 - ABZR87_RS13385 0.42 +1.0 2,836,974 - ABZR87_RS13385 0.42 -0.0 2,836,974 - ABZR87_RS13385 0.42 +0.9 2,836,974 - ABZR87_RS13385 0.42 -1.1 2,836,988 + ABZR87_RS13385 0.43 +0.8 2,836,988 + ABZR87_RS13385 0.43 +2.0 2,836,988 + ABZR87_RS13385 0.43 +0.7 2,836,988 + ABZR87_RS13385 0.43 -0.8 2,836,988 + ABZR87_RS13385 0.43 -0.7 2,836,988 + ABZR87_RS13385 0.43 -0.8 2,836,989 - ABZR87_RS13385 0.43 -0.4 2,836,989 - ABZR87_RS13385 0.43 -0.0 2,836,997 + ABZR87_RS13385 0.45 -1.2 2,837,060 + ABZR87_RS13385 0.53 -0.2 2,837,060 + ABZR87_RS13385 0.53 +0.3 2,837,060 + ABZR87_RS13385 0.53 -0.4 2,837,060 + ABZR87_RS13385 0.53 -0.1 2,837,060 + ABZR87_RS13385 0.53 -0.6 2,837,060 + ABZR87_RS13385 0.53 +0.2 2,837,195 + ABZR87_RS13385 0.70 -0.8 2,837,195 + ABZR87_RS13385 0.70 +0.4 2,837,195 + ABZR87_RS13385 0.70 +0.1 2,837,196 - ABZR87_RS13385 0.70 -0.2 2,837,196 - ABZR87_RS13385 0.70 -0.0 2,837,205 - ABZR87_RS13385 0.72 +3.6 2,837,240 + ABZR87_RS13385 0.76 -0.6 2,837,240 + ABZR87_RS13385 0.76 -0.2 2,837,240 + ABZR87_RS13385 0.76 +0.0 2,837,240 + ABZR87_RS13385 0.76 +0.4 2,837,240 + ABZR87_RS13385 0.76 +0.7 2,837,240 + ABZR87_RS13385 0.76 +0.1 2,837,240 + ABZR87_RS13385 0.76 -1.2 2,837,241 - ABZR87_RS13385 0.76 -0.2 2,837,241 - ABZR87_RS13385 0.76 -0.3 2,837,241 - ABZR87_RS13385 0.76 +0.3 2,837,241 - ABZR87_RS13385 0.76 +0.1 2,837,241 - ABZR87_RS13385 0.76 -0.4 2,837,241 - ABZR87_RS13385 0.76 +0.2 2,837,241 - ABZR87_RS13385 0.76 -0.8 2,837,288 + ABZR87_RS13385 0.82 +0.1 2,837,288 + ABZR87_RS13385 0.82 +0.4 2,837,288 + ABZR87_RS13385 0.82 -0.6 2,837,288 + ABZR87_RS13385 0.82 -0.2 2,837,289 - ABZR87_RS13385 0.83 -0.8 2,837,289 - ABZR87_RS13385 0.83 -0.3 2,837,289 - ABZR87_RS13385 0.83 +0.7 2,837,289 - ABZR87_RS13385 0.83 -0.7 2,837,289 - ABZR87_RS13385 0.83 +0.3 2,837,294 + ABZR87_RS13385 0.83 +2.0 2,837,295 - ABZR87_RS13385 0.83 +0.3 2,837,295 - ABZR87_RS13385 0.83 -0.6 2,837,333 + ABZR87_RS13385 0.88 -2.9 2,837,333 + ABZR87_RS13385 0.88 +0.6 2,837,333 + ABZR87_RS13385 0.88 +0.8 2,837,333 + ABZR87_RS13385 0.88 +0.1 2,837,333 + ABZR87_RS13385 0.88 -1.5 2,837,334 - ABZR87_RS13385 0.88 -0.5 2,837,463 + ABZR87_RS13390 0.24 -0.6 2,837,463 + ABZR87_RS13390 0.24 +0.1 2,837,463 + ABZR87_RS13390 0.24 -1.0 2,837,464 - ABZR87_RS13390 0.24 -0.8 2,837,490 + ABZR87_RS13390 0.40 -0.3 2,837,490 + ABZR87_RS13390 0.40 -0.7 2,837,730 + ABZR87_RS13395 0.40 +0.7 2,837,731 - ABZR87_RS13395 0.40 -0.8 2,837,731 - ABZR87_RS13395 0.40 +0.8 2,837,757 + ABZR87_RS13395 0.48 -0.3 2,837,758 - ABZR87_RS13395 0.49 -0.8 2,837,758 - ABZR87_RS13395 0.49 -0.1 2,837,810 + ABZR87_RS13395 0.65 -0.0 2,837,810 + ABZR87_RS13395 0.65 +0.3 2,837,811 - ABZR87_RS13395 0.65 -0.4 2,837,855 + ABZR87_RS13395 0.79 +1.2 2,837,855 + ABZR87_RS13395 0.79 -2.1 2,837,855 + ABZR87_RS13395 0.79 -0.3 2,837,855 + ABZR87_RS13395 0.79 +0.4 2,837,856 - ABZR87_RS13395 0.80 -0.1 2,837,856 - ABZR87_RS13395 0.80 +0.5 2,837,888 + -1.4 2,837,940 + -0.5 2,837,941 - +0.0 2,837,955 + +0.5 2,837,956 - -0.6 2,837,956 - +0.4 2,837,956 - -0.4 2,837,990 + -0.1 2,837,990 + +0.1 2,837,991 - +0.6 2,837,991 - -0.4 2,838,019 - ABZR87_RS13400 0.11 -0.9 2,838,292 + -2.3 2,838,292 + +0.2 2,838,292 + -0.5 2,838,292 + -1.5 2,838,338 + +1.0 2,838,338 + -0.6 2,838,339 - -0.3 2,838,425 + ABZR87_RS13405 0.11 -0.1 2,838,426 - ABZR87_RS13405 0.11 -1.7 2,838,436 + ABZR87_RS13405 0.12 -0.2 2,838,533 + ABZR87_RS13405 0.19 -0.2 2,838,533 + ABZR87_RS13405 0.19 -0.3 2,838,533 + ABZR87_RS13405 0.19 -1.4
Or see this region's nucleotide sequence