Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS13245 and ABZR87_RS13250 are separated by 110 nucleotides ABZR87_RS13250 and ABZR87_RS13255 are separated by 6 nucleotides
ABZR87_RS13245: ABZR87_RS13245 - acetylornithine deacetylase, at 2,807,326 to 2,808,519
_RS13245
ABZR87_RS13250: ABZR87_RS13250 - phosphoserine phosphatase SerB, at 2,808,630 to 2,809,487
_RS13250
ABZR87_RS13255: ABZR87_RS13255 - PDDEXK nuclease domain-containing protein, at 2,809,494 to 2,810,555
_RS13255
Position (kb)
2808
2809
2810 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2807.639 kb on - strand, within ABZR87_RS13245 at 2807.747 kb on - strand, within ABZR87_RS13245 at 2807.747 kb on - strand, within ABZR87_RS13245 at 2807.954 kb on - strand, within ABZR87_RS13245 at 2807.978 kb on + strand, within ABZR87_RS13245 at 2807.978 kb on + strand, within ABZR87_RS13245 at 2807.979 kb on - strand, within ABZR87_RS13245 at 2808.113 kb on + strand, within ABZR87_RS13245 at 2808.175 kb on + strand, within ABZR87_RS13245 at 2808.176 kb on - strand, within ABZR87_RS13245 at 2808.341 kb on - strand, within ABZR87_RS13245 at 2808.433 kb on + strand at 2808.433 kb on + strand at 2808.433 kb on + strand at 2808.434 kb on - strand at 2808.524 kb on + strand at 2808.524 kb on + strand at 2808.524 kb on + strand at 2808.525 kb on - strand at 2808.525 kb on - strand at 2808.577 kb on + strand at 2808.582 kb on + strand at 2808.582 kb on + strand at 2808.583 kb on - strand at 2808.711 kb on + strand at 2808.711 kb on + strand at 2808.712 kb on - strand at 2808.712 kb on - strand at 2808.816 kb on + strand, within ABZR87_RS13250 at 2808.944 kb on - strand, within ABZR87_RS13250 at 2808.944 kb on - strand, within ABZR87_RS13250 at 2809.273 kb on + strand, within ABZR87_RS13250 at 2809.486 kb on - strand at 2809.486 kb on - strand at 2809.486 kb on - strand at 2809.563 kb on + strand at 2809.564 kb on - strand at 2809.564 kb on - strand at 2809.611 kb on + strand, within ABZR87_RS13255 at 2809.611 kb on + strand, within ABZR87_RS13255 at 2809.611 kb on + strand, within ABZR87_RS13255 at 2809.611 kb on + strand, within ABZR87_RS13255 at 2809.612 kb on - strand, within ABZR87_RS13255 at 2809.612 kb on - strand, within ABZR87_RS13255 at 2809.612 kb on - strand, within ABZR87_RS13255 at 2809.645 kb on + strand, within ABZR87_RS13255 at 2809.812 kb on + strand, within ABZR87_RS13255 at 2809.812 kb on + strand, within ABZR87_RS13255 at 2809.813 kb on - strand, within ABZR87_RS13255 at 2809.813 kb on - strand, within ABZR87_RS13255 at 2809.813 kb on - strand, within ABZR87_RS13255 at 2809.813 kb on - strand, within ABZR87_RS13255 at 2809.866 kb on - strand, within ABZR87_RS13255 at 2809.914 kb on + strand, within ABZR87_RS13255 at 2809.914 kb on + strand, within ABZR87_RS13255 at 2810.073 kb on + strand, within ABZR87_RS13255 at 2810.073 kb on + strand, within ABZR87_RS13255 at 2810.073 kb on + strand, within ABZR87_RS13255 at 2810.074 kb on - strand, within ABZR87_RS13255 at 2810.074 kb on - strand, within ABZR87_RS13255 at 2810.074 kb on - strand, within ABZR87_RS13255 at 2810.074 kb on - strand, within ABZR87_RS13255 at 2810.074 kb on - strand, within ABZR87_RS13255 at 2810.074 kb on - strand, within ABZR87_RS13255 at 2810.226 kb on + strand, within ABZR87_RS13255 at 2810.227 kb on - strand, within ABZR87_RS13255 at 2810.245 kb on + strand, within ABZR87_RS13255 at 2810.245 kb on + strand, within ABZR87_RS13255 at 2810.246 kb on - strand, within ABZR87_RS13255 at 2810.246 kb on - strand, within ABZR87_RS13255 at 2810.331 kb on + strand, within ABZR87_RS13255 at 2810.331 kb on + strand, within ABZR87_RS13255 at 2810.332 kb on - strand, within ABZR87_RS13255 at 2810.332 kb on - strand, within ABZR87_RS13255 at 2810.428 kb on - strand, within ABZR87_RS13255 at 2810.469 kb on + strand at 2810.469 kb on + strand at 2810.469 kb on + strand at 2810.469 kb on + strand at 2810.469 kb on + strand at 2810.470 kb on - strand at 2810.470 kb on - strand at 2810.470 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,807,639 - ABZR87_RS13245 0.26 -1.4 2,807,747 - ABZR87_RS13245 0.35 +0.9 2,807,747 - ABZR87_RS13245 0.35 +0.4 2,807,954 - ABZR87_RS13245 0.53 -0.7 2,807,978 + ABZR87_RS13245 0.55 +0.4 2,807,978 + ABZR87_RS13245 0.55 +0.2 2,807,979 - ABZR87_RS13245 0.55 -0.3 2,808,113 + ABZR87_RS13245 0.66 -1.0 2,808,175 + ABZR87_RS13245 0.71 -0.1 2,808,176 - ABZR87_RS13245 0.71 +0.4 2,808,341 - ABZR87_RS13245 0.85 -1.3 2,808,433 + -0.7 2,808,433 + -0.4 2,808,433 + -0.1 2,808,434 - +0.1 2,808,524 + -0.5 2,808,524 + +1.0 2,808,524 + -0.2 2,808,525 - -0.4 2,808,525 - +0.4 2,808,577 + -1.2 2,808,582 + -0.3 2,808,582 + -0.2 2,808,583 - -1.0 2,808,711 + +0.7 2,808,711 + -0.8 2,808,712 - +0.4 2,808,712 - -2.1 2,808,816 + ABZR87_RS13250 0.22 -1.1 2,808,944 - ABZR87_RS13250 0.37 -0.7 2,808,944 - ABZR87_RS13250 0.37 +0.2 2,809,273 + ABZR87_RS13250 0.75 -0.6 2,809,486 - -1.1 2,809,486 - -0.6 2,809,486 - -1.2 2,809,563 + +0.3 2,809,564 - +0.5 2,809,564 - -0.6 2,809,611 + ABZR87_RS13255 0.11 -2.3 2,809,611 + ABZR87_RS13255 0.11 +0.4 2,809,611 + ABZR87_RS13255 0.11 -0.3 2,809,611 + ABZR87_RS13255 0.11 +1.3 2,809,612 - ABZR87_RS13255 0.11 +0.2 2,809,612 - ABZR87_RS13255 0.11 -0.2 2,809,612 - ABZR87_RS13255 0.11 -0.1 2,809,645 + ABZR87_RS13255 0.14 +0.3 2,809,812 + ABZR87_RS13255 0.30 -1.8 2,809,812 + ABZR87_RS13255 0.30 -0.0 2,809,813 - ABZR87_RS13255 0.30 +0.4 2,809,813 - ABZR87_RS13255 0.30 +0.9 2,809,813 - ABZR87_RS13255 0.30 -0.6 2,809,813 - ABZR87_RS13255 0.30 -0.5 2,809,866 - ABZR87_RS13255 0.35 -0.4 2,809,914 + ABZR87_RS13255 0.40 +0.0 2,809,914 + ABZR87_RS13255 0.40 -2.5 2,810,073 + ABZR87_RS13255 0.55 +0.3 2,810,073 + ABZR87_RS13255 0.55 -0.3 2,810,073 + ABZR87_RS13255 0.55 +0.1 2,810,074 - ABZR87_RS13255 0.55 -3.0 2,810,074 - ABZR87_RS13255 0.55 -1.4 2,810,074 - ABZR87_RS13255 0.55 +0.2 2,810,074 - ABZR87_RS13255 0.55 +0.3 2,810,074 - ABZR87_RS13255 0.55 +0.4 2,810,074 - ABZR87_RS13255 0.55 -0.0 2,810,226 + ABZR87_RS13255 0.69 -0.7 2,810,227 - ABZR87_RS13255 0.69 +0.0 2,810,245 + ABZR87_RS13255 0.71 +0.0 2,810,245 + ABZR87_RS13255 0.71 +0.1 2,810,246 - ABZR87_RS13255 0.71 +0.1 2,810,246 - ABZR87_RS13255 0.71 -0.6 2,810,331 + ABZR87_RS13255 0.79 +0.7 2,810,331 + ABZR87_RS13255 0.79 +0.3 2,810,332 - ABZR87_RS13255 0.79 -0.9 2,810,332 - ABZR87_RS13255 0.79 +0.2 2,810,428 - ABZR87_RS13255 0.88 -0.1 2,810,469 + -0.1 2,810,469 + -0.9 2,810,469 + -1.4 2,810,469 + +0.3 2,810,469 + -0.6 2,810,470 - -0.7 2,810,470 - +1.0 2,810,470 - -0.2
Or see this region's nucleotide sequence