Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS11165 and ABZR87_RS11170 are separated by 35 nucleotides ABZR87_RS11170 and ABZR87_RS11175 overlap by 4 nucleotides ABZR87_RS11175 and ABZR87_RS11180 are separated by 21 nucleotides ABZR87_RS11180 and ABZR87_RS11185 are separated by 179 nucleotides
ABZR87_RS11165: ABZR87_RS11165 - cytochrome c oxidase accessory protein CcoG, at 2,369,424 to 2,370,887
_RS11165
ABZR87_RS11170: ABZR87_RS11170 - FixH family protein, at 2,370,923 to 2,371,150
_RS11170
ABZR87_RS11175: ABZR87_RS11175 - hypothetical protein, at 2,371,147 to 2,371,401
_RS11175
ABZR87_RS11180: ABZR87_RS11180 - fumarate/nitrate reduction transcriptional regulator Fnr, at 2,371,423 to 2,372,151
_RS11180
ABZR87_RS11185: ABZR87_RS11185 - sulfite exporter TauE/SafE family protein, at 2,372,331 to 2,373,074
_RS11185
Position (kb)
2371
2372
2373 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2370.480 kb on + strand, within ABZR87_RS11165 at 2370.480 kb on + strand, within ABZR87_RS11165 at 2371.073 kb on - strand, within ABZR87_RS11170 at 2371.229 kb on + strand, within ABZR87_RS11175 at 2371.229 kb on + strand, within ABZR87_RS11175 at 2371.230 kb on - strand, within ABZR87_RS11175 at 2371.230 kb on - strand, within ABZR87_RS11175 at 2371.230 kb on - strand, within ABZR87_RS11175 at 2371.230 kb on - strand, within ABZR87_RS11175 at 2371.230 kb on - strand, within ABZR87_RS11175 at 2371.288 kb on + strand, within ABZR87_RS11175 at 2371.289 kb on - strand, within ABZR87_RS11175 at 2371.289 kb on - strand, within ABZR87_RS11175 at 2371.289 kb on - strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.349 kb on - strand, within ABZR87_RS11175 at 2371.349 kb on - strand, within ABZR87_RS11175 at 2371.349 kb on - strand, within ABZR87_RS11175 at 2371.349 kb on - strand, within ABZR87_RS11175 at 2371.349 kb on - strand, within ABZR87_RS11175 at 2371.352 kb on + strand, within ABZR87_RS11175 at 2371.353 kb on - strand, within ABZR87_RS11175 at 2371.419 kb on + strand at 2371.420 kb on - strand at 2371.563 kb on - strand, within ABZR87_RS11180 at 2371.852 kb on - strand, within ABZR87_RS11180 at 2371.852 kb on - strand, within ABZR87_RS11180 at 2371.964 kb on + strand, within ABZR87_RS11180 at 2371.964 kb on + strand, within ABZR87_RS11180 at 2371.964 kb on + strand, within ABZR87_RS11180 at 2371.965 kb on - strand, within ABZR87_RS11180 at 2371.965 kb on - strand, within ABZR87_RS11180 at 2371.994 kb on + strand, within ABZR87_RS11180 at 2371.994 kb on + strand, within ABZR87_RS11180 at 2371.995 kb on - strand, within ABZR87_RS11180 at 2371.995 kb on - strand, within ABZR87_RS11180 at 2372.014 kb on - strand, within ABZR87_RS11180 at 2372.014 kb on - strand, within ABZR87_RS11180 at 2372.099 kb on - strand at 2372.130 kb on + strand at 2372.188 kb on + strand at 2372.212 kb on + strand at 2372.212 kb on + strand at 2372.212 kb on + strand at 2372.213 kb on - strand at 2372.213 kb on - strand at 2372.213 kb on - strand at 2372.213 kb on - strand at 2372.215 kb on + strand at 2372.215 kb on + strand at 2372.215 kb on + strand at 2372.216 kb on - strand at 2372.216 kb on - strand at 2372.270 kb on + strand at 2372.270 kb on + strand at 2372.270 kb on + strand at 2372.271 kb on - strand at 2372.511 kb on + strand, within ABZR87_RS11185 at 2372.511 kb on + strand, within ABZR87_RS11185 at 2372.511 kb on + strand, within ABZR87_RS11185 at 2372.511 kb on + strand, within ABZR87_RS11185 at 2372.511 kb on + strand, within ABZR87_RS11185 at 2372.511 kb on + strand, within ABZR87_RS11185 at 2372.512 kb on - strand, within ABZR87_RS11185 at 2372.512 kb on - strand, within ABZR87_RS11185 at 2372.512 kb on - strand, within ABZR87_RS11185 at 2372.512 kb on - strand, within ABZR87_RS11185 at 2372.512 kb on - strand, within ABZR87_RS11185 at 2372.512 kb on - strand, within ABZR87_RS11185 at 2372.512 kb on - strand, within ABZR87_RS11185 at 2372.614 kb on + strand, within ABZR87_RS11185 at 2372.614 kb on + strand, within ABZR87_RS11185 at 2372.615 kb on - strand, within ABZR87_RS11185 at 2372.615 kb on - strand, within ABZR87_RS11185 at 2372.615 kb on - strand, within ABZR87_RS11185 at 2372.623 kb on - strand, within ABZR87_RS11185 at 2372.657 kb on + strand, within ABZR87_RS11185 at 2372.772 kb on + strand, within ABZR87_RS11185 at 2372.772 kb on + strand, within ABZR87_RS11185 at 2372.772 kb on + strand, within ABZR87_RS11185 at 2372.772 kb on + strand, within ABZR87_RS11185 at 2372.774 kb on + strand, within ABZR87_RS11185 at 2372.774 kb on + strand, within ABZR87_RS11185 at 2372.774 kb on + strand, within ABZR87_RS11185 at 2372.775 kb on - strand, within ABZR87_RS11185 at 2372.775 kb on - strand, within ABZR87_RS11185 at 2372.775 kb on - strand, within ABZR87_RS11185 at 2372.817 kb on + strand, within ABZR87_RS11185 at 2372.817 kb on + strand, within ABZR87_RS11185 at 2372.818 kb on - strand, within ABZR87_RS11185 at 2372.818 kb on - strand, within ABZR87_RS11185 at 2372.908 kb on + strand, within ABZR87_RS11185 at 2372.908 kb on + strand, within ABZR87_RS11185 at 2372.909 kb on - strand, within ABZR87_RS11185 at 2372.909 kb on - strand, within ABZR87_RS11185 at 2372.909 kb on - strand, within ABZR87_RS11185 at 2373.072 kb on + strand at 2373.072 kb on + strand at 2373.072 kb on + strand at 2373.072 kb on + strand at 2373.072 kb on + strand at 2373.073 kb on - strand at 2373.073 kb on - strand at 2373.073 kb on - strand at 2373.073 kb on - strand at 2373.073 kb on - strand at 2373.073 kb on - strand at 2373.080 kb on + strand at 2373.080 kb on + strand at 2373.081 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,370,480 + ABZR87_RS11165 0.72 -0.6 2,370,480 + ABZR87_RS11165 0.72 +0.2 2,371,073 - ABZR87_RS11170 0.66 -1.2 2,371,229 + ABZR87_RS11175 0.32 -0.4 2,371,229 + ABZR87_RS11175 0.32 -0.3 2,371,230 - ABZR87_RS11175 0.33 -1.5 2,371,230 - ABZR87_RS11175 0.33 -1.2 2,371,230 - ABZR87_RS11175 0.33 +0.9 2,371,230 - ABZR87_RS11175 0.33 -0.7 2,371,230 - ABZR87_RS11175 0.33 +0.2 2,371,288 + ABZR87_RS11175 0.55 +0.4 2,371,289 - ABZR87_RS11175 0.56 -0.6 2,371,289 - ABZR87_RS11175 0.56 +1.5 2,371,289 - ABZR87_RS11175 0.56 +0.6 2,371,348 + ABZR87_RS11175 0.79 +0.6 2,371,348 + ABZR87_RS11175 0.79 +0.2 2,371,348 + ABZR87_RS11175 0.79 +1.8 2,371,348 + ABZR87_RS11175 0.79 +1.2 2,371,348 + ABZR87_RS11175 0.79 +2.0 2,371,348 + ABZR87_RS11175 0.79 -0.3 2,371,348 + ABZR87_RS11175 0.79 -1.3 2,371,348 + ABZR87_RS11175 0.79 -0.2 2,371,348 + ABZR87_RS11175 0.79 +0.1 2,371,349 - ABZR87_RS11175 0.79 +1.3 2,371,349 - ABZR87_RS11175 0.79 +0.9 2,371,349 - ABZR87_RS11175 0.79 -0.5 2,371,349 - ABZR87_RS11175 0.79 -0.2 2,371,349 - ABZR87_RS11175 0.79 +0.4 2,371,352 + ABZR87_RS11175 0.80 -0.3 2,371,353 - ABZR87_RS11175 0.81 -2.3 2,371,419 + -0.4 2,371,420 - +0.0 2,371,563 - ABZR87_RS11180 0.19 +1.0 2,371,852 - ABZR87_RS11180 0.59 -2.1 2,371,852 - ABZR87_RS11180 0.59 +0.4 2,371,964 + ABZR87_RS11180 0.74 -0.3 2,371,964 + ABZR87_RS11180 0.74 +0.0 2,371,964 + ABZR87_RS11180 0.74 +0.7 2,371,965 - ABZR87_RS11180 0.74 +0.7 2,371,965 - ABZR87_RS11180 0.74 +1.5 2,371,994 + ABZR87_RS11180 0.78 -0.5 2,371,994 + ABZR87_RS11180 0.78 -0.5 2,371,995 - ABZR87_RS11180 0.78 +0.4 2,371,995 - ABZR87_RS11180 0.78 +0.1 2,372,014 - ABZR87_RS11180 0.81 +0.2 2,372,014 - ABZR87_RS11180 0.81 +2.6 2,372,099 - +0.4 2,372,130 + +1.9 2,372,188 + +0.7 2,372,212 + +0.0 2,372,212 + +1.3 2,372,212 + -0.0 2,372,213 - +1.3 2,372,213 - -2.7 2,372,213 - +0.5 2,372,213 - -0.2 2,372,215 + +1.0 2,372,215 + -0.5 2,372,215 + -0.4 2,372,216 - -0.4 2,372,216 - -0.1 2,372,270 + -1.8 2,372,270 + -0.4 2,372,270 + +1.0 2,372,271 - -0.6 2,372,511 + ABZR87_RS11185 0.24 -2.0 2,372,511 + ABZR87_RS11185 0.24 +1.5 2,372,511 + ABZR87_RS11185 0.24 -2.2 2,372,511 + ABZR87_RS11185 0.24 +0.7 2,372,511 + ABZR87_RS11185 0.24 +1.4 2,372,511 + ABZR87_RS11185 0.24 +0.5 2,372,512 - ABZR87_RS11185 0.24 +1.3 2,372,512 - ABZR87_RS11185 0.24 +1.4 2,372,512 - ABZR87_RS11185 0.24 -0.1 2,372,512 - ABZR87_RS11185 0.24 -0.2 2,372,512 - ABZR87_RS11185 0.24 -0.4 2,372,512 - ABZR87_RS11185 0.24 +2.0 2,372,512 - ABZR87_RS11185 0.24 +0.1 2,372,614 + ABZR87_RS11185 0.38 +0.2 2,372,614 + ABZR87_RS11185 0.38 +0.9 2,372,615 - ABZR87_RS11185 0.38 -0.2 2,372,615 - ABZR87_RS11185 0.38 +0.3 2,372,615 - ABZR87_RS11185 0.38 +0.3 2,372,623 - ABZR87_RS11185 0.39 +0.2 2,372,657 + ABZR87_RS11185 0.44 -1.8 2,372,772 + ABZR87_RS11185 0.59 -0.1 2,372,772 + ABZR87_RS11185 0.59 +0.8 2,372,772 + ABZR87_RS11185 0.59 -0.4 2,372,772 + ABZR87_RS11185 0.59 -0.1 2,372,774 + ABZR87_RS11185 0.60 +0.5 2,372,774 + ABZR87_RS11185 0.60 +0.1 2,372,774 + ABZR87_RS11185 0.60 -0.2 2,372,775 - ABZR87_RS11185 0.60 +0.5 2,372,775 - ABZR87_RS11185 0.60 -0.4 2,372,775 - ABZR87_RS11185 0.60 +0.6 2,372,817 + ABZR87_RS11185 0.65 +0.5 2,372,817 + ABZR87_RS11185 0.65 -0.3 2,372,818 - ABZR87_RS11185 0.65 +0.5 2,372,818 - ABZR87_RS11185 0.65 -1.1 2,372,908 + ABZR87_RS11185 0.78 +0.6 2,372,908 + ABZR87_RS11185 0.78 +4.0 2,372,909 - ABZR87_RS11185 0.78 +0.7 2,372,909 - ABZR87_RS11185 0.78 +2.0 2,372,909 - ABZR87_RS11185 0.78 +1.1 2,373,072 + -0.6 2,373,072 + -0.3 2,373,072 + -0.7 2,373,072 + +0.4 2,373,072 + -1.6 2,373,073 - +1.2 2,373,073 - -0.0 2,373,073 - +0.9 2,373,073 - -2.7 2,373,073 - +0.9 2,373,073 - -0.7 2,373,080 + -0.2 2,373,080 + -0.6 2,373,081 - -0.6
Or see this region's nucleotide sequence