Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS11155 and ABZR87_RS11160 are separated by 12 nucleotides ABZR87_RS11160 and ABZR87_RS11165 overlap by 4 nucleotides ABZR87_RS11165 and ABZR87_RS11170 are separated by 35 nucleotides ABZR87_RS11170 and ABZR87_RS11175 overlap by 4 nucleotides ABZR87_RS11175 and ABZR87_RS11180 are separated by 21 nucleotides
ABZR87_RS11155: ABZR87_RS11155 - cbb3-type cytochrome oxidase subunit 3, at 2,368,309 to 2,368,491
_RS11155
ABZR87_RS11160: ABZR87_RS11160 - cytochrome-c oxidase, cbb3-type subunit III, at 2,368,504 to 2,369,427
_RS11160
ABZR87_RS11165: ABZR87_RS11165 - cytochrome c oxidase accessory protein CcoG, at 2,369,424 to 2,370,887
_RS11165
ABZR87_RS11170: ABZR87_RS11170 - FixH family protein, at 2,370,923 to 2,371,150
_RS11170
ABZR87_RS11175: ABZR87_RS11175 - hypothetical protein, at 2,371,147 to 2,371,401
_RS11175
ABZR87_RS11180: ABZR87_RS11180 - fumarate/nitrate reduction transcriptional regulator Fnr, at 2,371,423 to 2,372,151
_RS11180
Position (kb)
2369
2370
2371 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2368.489 kb on + strand at 2368.489 kb on + strand at 2368.490 kb on - strand at 2368.490 kb on - strand at 2368.534 kb on + strand at 2368.534 kb on + strand at 2368.535 kb on - strand at 2368.537 kb on + strand at 2368.537 kb on + strand at 2368.537 kb on + strand at 2368.537 kb on + strand at 2368.538 kb on - strand at 2368.538 kb on - strand at 2368.538 kb on - strand at 2368.538 kb on - strand at 2368.538 kb on - strand at 2368.538 kb on - strand at 2368.739 kb on - strand, within ABZR87_RS11160 at 2368.768 kb on + strand, within ABZR87_RS11160 at 2368.768 kb on + strand, within ABZR87_RS11160 at 2368.769 kb on - strand, within ABZR87_RS11160 at 2368.850 kb on - strand, within ABZR87_RS11160 at 2368.850 kb on - strand, within ABZR87_RS11160 at 2368.858 kb on + strand, within ABZR87_RS11160 at 2369.119 kb on + strand, within ABZR87_RS11160 at 2369.119 kb on + strand, within ABZR87_RS11160 at 2369.120 kb on - strand, within ABZR87_RS11160 at 2369.272 kb on + strand, within ABZR87_RS11160 at 2369.273 kb on - strand, within ABZR87_RS11160 at 2369.273 kb on - strand, within ABZR87_RS11160 at 2369.384 kb on - strand at 2369.384 kb on - strand at 2369.403 kb on + strand at 2369.404 kb on - strand at 2369.512 kb on - strand at 2369.532 kb on + strand at 2369.532 kb on + strand at 2369.604 kb on + strand, within ABZR87_RS11165 at 2369.604 kb on + strand, within ABZR87_RS11165 at 2369.605 kb on - strand, within ABZR87_RS11165 at 2369.674 kb on - strand, within ABZR87_RS11165 at 2369.674 kb on - strand, within ABZR87_RS11165 at 2369.713 kb on - strand, within ABZR87_RS11165 at 2369.811 kb on + strand, within ABZR87_RS11165 at 2369.811 kb on + strand, within ABZR87_RS11165 at 2369.812 kb on - strand, within ABZR87_RS11165 at 2369.950 kb on + strand, within ABZR87_RS11165 at 2369.951 kb on - strand, within ABZR87_RS11165 at 2369.951 kb on - strand, within ABZR87_RS11165 at 2370.057 kb on + strand, within ABZR87_RS11165 at 2370.058 kb on - strand, within ABZR87_RS11165 at 2370.058 kb on - strand, within ABZR87_RS11165 at 2370.058 kb on - strand, within ABZR87_RS11165 at 2370.111 kb on + strand, within ABZR87_RS11165 at 2370.111 kb on + strand, within ABZR87_RS11165 at 2370.159 kb on - strand, within ABZR87_RS11165 at 2370.162 kb on + strand, within ABZR87_RS11165 at 2370.163 kb on - strand, within ABZR87_RS11165 at 2370.163 kb on - strand, within ABZR87_RS11165 at 2370.163 kb on - strand, within ABZR87_RS11165 at 2370.175 kb on - strand, within ABZR87_RS11165 at 2370.316 kb on - strand, within ABZR87_RS11165 at 2370.316 kb on - strand, within ABZR87_RS11165 at 2370.316 kb on - strand, within ABZR87_RS11165 at 2370.400 kb on - strand, within ABZR87_RS11165 at 2370.401 kb on + strand, within ABZR87_RS11165 at 2370.402 kb on - strand, within ABZR87_RS11165 at 2370.402 kb on - strand, within ABZR87_RS11165 at 2370.402 kb on - strand, within ABZR87_RS11165 at 2370.402 kb on - strand, within ABZR87_RS11165 at 2370.402 kb on - strand, within ABZR87_RS11165 at 2370.402 kb on - strand, within ABZR87_RS11165 at 2370.402 kb on - strand, within ABZR87_RS11165 at 2370.480 kb on + strand, within ABZR87_RS11165 at 2370.480 kb on + strand, within ABZR87_RS11165 at 2371.073 kb on - strand, within ABZR87_RS11170 at 2371.229 kb on + strand, within ABZR87_RS11175 at 2371.229 kb on + strand, within ABZR87_RS11175 at 2371.230 kb on - strand, within ABZR87_RS11175 at 2371.230 kb on - strand, within ABZR87_RS11175 at 2371.230 kb on - strand, within ABZR87_RS11175 at 2371.230 kb on - strand, within ABZR87_RS11175 at 2371.230 kb on - strand, within ABZR87_RS11175 at 2371.288 kb on + strand, within ABZR87_RS11175 at 2371.289 kb on - strand, within ABZR87_RS11175 at 2371.289 kb on - strand, within ABZR87_RS11175 at 2371.289 kb on - strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.348 kb on + strand, within ABZR87_RS11175 at 2371.349 kb on - strand, within ABZR87_RS11175 at 2371.349 kb on - strand, within ABZR87_RS11175 at 2371.349 kb on - strand, within ABZR87_RS11175 at 2371.349 kb on - strand, within ABZR87_RS11175 at 2371.349 kb on - strand, within ABZR87_RS11175 at 2371.352 kb on + strand, within ABZR87_RS11175 at 2371.353 kb on - strand, within ABZR87_RS11175 at 2371.419 kb on + strand at 2371.420 kb on - strand at 2371.563 kb on - strand, within ABZR87_RS11180 at 2371.852 kb on - strand, within ABZR87_RS11180 at 2371.852 kb on - strand, within ABZR87_RS11180
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,368,489 + +0.4 2,368,489 + +0.1 2,368,490 - +0.2 2,368,490 - -1.2 2,368,534 + -1.2 2,368,534 + -1.1 2,368,535 - +1.7 2,368,537 + -0.3 2,368,537 + -0.6 2,368,537 + +0.4 2,368,537 + +2.3 2,368,538 - -1.3 2,368,538 - +0.4 2,368,538 - -0.4 2,368,538 - +1.0 2,368,538 - -0.9 2,368,538 - +1.4 2,368,739 - ABZR87_RS11160 0.25 +0.8 2,368,768 + ABZR87_RS11160 0.29 +0.7 2,368,768 + ABZR87_RS11160 0.29 +0.6 2,368,769 - ABZR87_RS11160 0.29 +1.0 2,368,850 - ABZR87_RS11160 0.37 -2.4 2,368,850 - ABZR87_RS11160 0.37 +0.3 2,368,858 + ABZR87_RS11160 0.38 -0.1 2,369,119 + ABZR87_RS11160 0.67 +0.2 2,369,119 + ABZR87_RS11160 0.67 -0.7 2,369,120 - ABZR87_RS11160 0.67 +0.0 2,369,272 + ABZR87_RS11160 0.83 +0.0 2,369,273 - ABZR87_RS11160 0.83 -1.3 2,369,273 - ABZR87_RS11160 0.83 +0.6 2,369,384 - +0.2 2,369,384 - +0.6 2,369,403 + -0.1 2,369,404 - -0.2 2,369,512 - -0.1 2,369,532 + -0.2 2,369,532 + -0.4 2,369,604 + ABZR87_RS11165 0.12 -0.4 2,369,604 + ABZR87_RS11165 0.12 +0.3 2,369,605 - ABZR87_RS11165 0.12 +0.5 2,369,674 - ABZR87_RS11165 0.17 -0.4 2,369,674 - ABZR87_RS11165 0.17 +0.0 2,369,713 - ABZR87_RS11165 0.20 +2.3 2,369,811 + ABZR87_RS11165 0.26 -0.0 2,369,811 + ABZR87_RS11165 0.26 +0.4 2,369,812 - ABZR87_RS11165 0.27 +1.3 2,369,950 + ABZR87_RS11165 0.36 +0.8 2,369,951 - ABZR87_RS11165 0.36 +0.9 2,369,951 - ABZR87_RS11165 0.36 -0.0 2,370,057 + ABZR87_RS11165 0.43 -0.1 2,370,058 - ABZR87_RS11165 0.43 -1.5 2,370,058 - ABZR87_RS11165 0.43 -0.3 2,370,058 - ABZR87_RS11165 0.43 +0.3 2,370,111 + ABZR87_RS11165 0.47 -0.1 2,370,111 + ABZR87_RS11165 0.47 -0.8 2,370,159 - ABZR87_RS11165 0.50 +0.7 2,370,162 + ABZR87_RS11165 0.50 +1.0 2,370,163 - ABZR87_RS11165 0.50 -1.9 2,370,163 - ABZR87_RS11165 0.50 +0.4 2,370,163 - ABZR87_RS11165 0.50 +1.7 2,370,175 - ABZR87_RS11165 0.51 +0.0 2,370,316 - ABZR87_RS11165 0.61 -0.8 2,370,316 - ABZR87_RS11165 0.61 +1.4 2,370,316 - ABZR87_RS11165 0.61 -1.4 2,370,400 - ABZR87_RS11165 0.67 +2.8 2,370,401 + ABZR87_RS11165 0.67 -0.9 2,370,402 - ABZR87_RS11165 0.67 -0.2 2,370,402 - ABZR87_RS11165 0.67 +1.5 2,370,402 - ABZR87_RS11165 0.67 +0.3 2,370,402 - ABZR87_RS11165 0.67 +0.5 2,370,402 - ABZR87_RS11165 0.67 -1.5 2,370,402 - ABZR87_RS11165 0.67 +0.5 2,370,402 - ABZR87_RS11165 0.67 +0.4 2,370,480 + ABZR87_RS11165 0.72 -0.6 2,370,480 + ABZR87_RS11165 0.72 +0.2 2,371,073 - ABZR87_RS11170 0.66 -1.2 2,371,229 + ABZR87_RS11175 0.32 -0.4 2,371,229 + ABZR87_RS11175 0.32 -0.3 2,371,230 - ABZR87_RS11175 0.33 -1.5 2,371,230 - ABZR87_RS11175 0.33 -1.2 2,371,230 - ABZR87_RS11175 0.33 +0.9 2,371,230 - ABZR87_RS11175 0.33 -0.7 2,371,230 - ABZR87_RS11175 0.33 +0.2 2,371,288 + ABZR87_RS11175 0.55 +0.4 2,371,289 - ABZR87_RS11175 0.56 -0.6 2,371,289 - ABZR87_RS11175 0.56 +1.5 2,371,289 - ABZR87_RS11175 0.56 +0.6 2,371,348 + ABZR87_RS11175 0.79 +0.6 2,371,348 + ABZR87_RS11175 0.79 +0.2 2,371,348 + ABZR87_RS11175 0.79 +1.8 2,371,348 + ABZR87_RS11175 0.79 +1.2 2,371,348 + ABZR87_RS11175 0.79 +2.0 2,371,348 + ABZR87_RS11175 0.79 -0.3 2,371,348 + ABZR87_RS11175 0.79 -1.3 2,371,348 + ABZR87_RS11175 0.79 -0.2 2,371,348 + ABZR87_RS11175 0.79 +0.1 2,371,349 - ABZR87_RS11175 0.79 +1.3 2,371,349 - ABZR87_RS11175 0.79 +0.9 2,371,349 - ABZR87_RS11175 0.79 -0.5 2,371,349 - ABZR87_RS11175 0.79 -0.2 2,371,349 - ABZR87_RS11175 0.79 +0.4 2,371,352 + ABZR87_RS11175 0.80 -0.3 2,371,353 - ABZR87_RS11175 0.81 -2.3 2,371,419 + -0.4 2,371,420 - +0.0 2,371,563 - ABZR87_RS11180 0.19 +1.0 2,371,852 - ABZR87_RS11180 0.59 -2.1 2,371,852 - ABZR87_RS11180 0.59 +0.4
Or see this region's nucleotide sequence