Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS11120 and ABZR87_RS11125 are separated by 101 nucleotides ABZR87_RS11125 and ABZR87_RS11130 are separated by 27 nucleotides
ABZR87_RS11120: ABZR87_RS11120 - dihydrolipoyl dehydrogenase, at 2,359,630 to 2,361,066
_RS11120
ABZR87_RS11125: ABZR87_RS11125 - cell division protein ZapE, at 2,361,168 to 2,362,265
_RS11125
ABZR87_RS11130: ABZR87_RS11130 - 2-phosphosulfolactate phosphatase, at 2,362,293 to 2,363,135
_RS11130
Position (kb)
2361
2362
2363 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2361.060 kb on + strand at 2361.060 kb on + strand at 2361.060 kb on + strand at 2361.064 kb on + strand at 2361.065 kb on - strand at 2361.186 kb on + strand at 2361.187 kb on - strand at 2361.187 kb on - strand at 2361.190 kb on + strand at 2361.191 kb on - strand at 2361.191 kb on - strand at 2361.213 kb on + strand at 2361.348 kb on + strand, within ABZR87_RS11125 at 2361.349 kb on - strand, within ABZR87_RS11125 at 2361.349 kb on - strand, within ABZR87_RS11125 at 2361.399 kb on + strand, within ABZR87_RS11125 at 2361.399 kb on + strand, within ABZR87_RS11125 at 2361.399 kb on + strand, within ABZR87_RS11125 at 2361.400 kb on - strand, within ABZR87_RS11125 at 2361.400 kb on - strand, within ABZR87_RS11125 at 2361.400 kb on - strand, within ABZR87_RS11125 at 2361.400 kb on - strand, within ABZR87_RS11125 at 2361.400 kb on - strand, within ABZR87_RS11125 at 2361.400 kb on - strand, within ABZR87_RS11125 at 2361.401 kb on + strand, within ABZR87_RS11125 at 2361.402 kb on - strand, within ABZR87_RS11125 at 2361.402 kb on - strand, within ABZR87_RS11125 at 2361.795 kb on + strand, within ABZR87_RS11125 at 2361.796 kb on - strand, within ABZR87_RS11125 at 2361.796 kb on - strand, within ABZR87_RS11125 at 2361.796 kb on - strand, within ABZR87_RS11125 at 2361.796 kb on - strand, within ABZR87_RS11125 at 2361.798 kb on + strand, within ABZR87_RS11125 at 2361.799 kb on - strand, within ABZR87_RS11125 at 2361.799 kb on - strand, within ABZR87_RS11125 at 2361.845 kb on + strand, within ABZR87_RS11125 at 2361.846 kb on + strand, within ABZR87_RS11125 at 2361.917 kb on + strand, within ABZR87_RS11125 at 2361.984 kb on + strand, within ABZR87_RS11125 at 2362.095 kb on + strand, within ABZR87_RS11125 at 2362.096 kb on - strand, within ABZR87_RS11125 at 2362.150 kb on - strand, within ABZR87_RS11125 at 2362.250 kb on + strand at 2362.250 kb on + strand at 2362.250 kb on + strand at 2362.250 kb on + strand at 2362.251 kb on - strand at 2362.251 kb on - strand at 2362.251 kb on - strand at 2362.295 kb on - strand at 2362.295 kb on - strand at 2362.295 kb on - strand at 2362.558 kb on + strand, within ABZR87_RS11130 at 2362.558 kb on + strand, within ABZR87_RS11130 at 2362.559 kb on - strand, within ABZR87_RS11130 at 2362.889 kb on + strand, within ABZR87_RS11130 at 2362.889 kb on + strand, within ABZR87_RS11130 at 2362.889 kb on + strand, within ABZR87_RS11130 at 2362.890 kb on - strand, within ABZR87_RS11130 at 2362.890 kb on - strand, within ABZR87_RS11130 at 2362.890 kb on - strand, within ABZR87_RS11130 at 2362.890 kb on - strand, within ABZR87_RS11130 at 2362.890 kb on - strand, within ABZR87_RS11130 at 2362.890 kb on - strand, within ABZR87_RS11130 at 2362.891 kb on + strand, within ABZR87_RS11130 at 2362.891 kb on + strand, within ABZR87_RS11130 at 2362.891 kb on + strand, within ABZR87_RS11130 at 2362.981 kb on + strand, within ABZR87_RS11130 at 2362.981 kb on + strand, within ABZR87_RS11130 at 2362.981 kb on + strand, within ABZR87_RS11130 at 2362.982 kb on - strand, within ABZR87_RS11130 at 2362.982 kb on - strand, within ABZR87_RS11130 at 2362.982 kb on - strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.985 kb on + strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2362.986 kb on - strand, within ABZR87_RS11130 at 2363.113 kb on + strand at 2363.113 kb on + strand at 2363.113 kb on + strand at 2363.113 kb on + strand at 2363.114 kb on - strand at 2363.172 kb on + strand at 2363.172 kb on + strand at 2363.175 kb on - strand at 2363.175 kb on - strand at 2363.175 kb on - strand at 2363.175 kb on - strand at 2363.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,361,060 + +0.1 2,361,060 + -0.1 2,361,060 + -0.1 2,361,064 + +0.4 2,361,065 - +0.1 2,361,186 + +0.0 2,361,187 - +1.8 2,361,187 - +1.9 2,361,190 + +0.2 2,361,191 - +0.8 2,361,191 - +0.0 2,361,213 + -0.6 2,361,348 + ABZR87_RS11125 0.16 -0.3 2,361,349 - ABZR87_RS11125 0.16 +1.7 2,361,349 - ABZR87_RS11125 0.16 -1.3 2,361,399 + ABZR87_RS11125 0.21 -0.8 2,361,399 + ABZR87_RS11125 0.21 +0.2 2,361,399 + ABZR87_RS11125 0.21 +0.0 2,361,400 - ABZR87_RS11125 0.21 -1.6 2,361,400 - ABZR87_RS11125 0.21 +1.0 2,361,400 - ABZR87_RS11125 0.21 -0.8 2,361,400 - ABZR87_RS11125 0.21 -1.0 2,361,400 - ABZR87_RS11125 0.21 -0.8 2,361,400 - ABZR87_RS11125 0.21 -0.1 2,361,401 + ABZR87_RS11125 0.21 +1.3 2,361,402 - ABZR87_RS11125 0.21 -0.7 2,361,402 - ABZR87_RS11125 0.21 -2.8 2,361,795 + ABZR87_RS11125 0.57 -2.0 2,361,796 - ABZR87_RS11125 0.57 +0.4 2,361,796 - ABZR87_RS11125 0.57 +0.4 2,361,796 - ABZR87_RS11125 0.57 +0.4 2,361,796 - ABZR87_RS11125 0.57 +1.0 2,361,798 + ABZR87_RS11125 0.57 +1.3 2,361,799 - ABZR87_RS11125 0.57 -1.7 2,361,799 - ABZR87_RS11125 0.57 -0.6 2,361,845 + ABZR87_RS11125 0.62 -0.6 2,361,846 + ABZR87_RS11125 0.62 +0.9 2,361,917 + ABZR87_RS11125 0.68 -0.3 2,361,984 + ABZR87_RS11125 0.74 -2.1 2,362,095 + ABZR87_RS11125 0.84 +0.0 2,362,096 - ABZR87_RS11125 0.85 +0.1 2,362,150 - ABZR87_RS11125 0.89 +1.2 2,362,250 + -1.5 2,362,250 + -0.6 2,362,250 + +0.8 2,362,250 + +0.6 2,362,251 - -1.9 2,362,251 - +0.2 2,362,251 - -0.3 2,362,295 - +0.1 2,362,295 - -0.2 2,362,295 - -0.1 2,362,558 + ABZR87_RS11130 0.31 -0.3 2,362,558 + ABZR87_RS11130 0.31 +0.3 2,362,559 - ABZR87_RS11130 0.32 +0.4 2,362,889 + ABZR87_RS11130 0.71 -0.3 2,362,889 + ABZR87_RS11130 0.71 +0.5 2,362,889 + ABZR87_RS11130 0.71 -1.0 2,362,890 - ABZR87_RS11130 0.71 -1.8 2,362,890 - ABZR87_RS11130 0.71 -1.3 2,362,890 - ABZR87_RS11130 0.71 -0.2 2,362,890 - ABZR87_RS11130 0.71 -0.1 2,362,890 - ABZR87_RS11130 0.71 -0.3 2,362,890 - ABZR87_RS11130 0.71 -0.2 2,362,891 + ABZR87_RS11130 0.71 -0.2 2,362,891 + ABZR87_RS11130 0.71 -1.0 2,362,891 + ABZR87_RS11130 0.71 -1.2 2,362,981 + ABZR87_RS11130 0.82 -1.8 2,362,981 + ABZR87_RS11130 0.82 +1.3 2,362,981 + ABZR87_RS11130 0.82 +0.6 2,362,982 - ABZR87_RS11130 0.82 -1.7 2,362,982 - ABZR87_RS11130 0.82 -0.8 2,362,982 - ABZR87_RS11130 0.82 -0.4 2,362,985 + ABZR87_RS11130 0.82 -0.5 2,362,985 + ABZR87_RS11130 0.82 -0.2 2,362,985 + ABZR87_RS11130 0.82 -2.0 2,362,985 + ABZR87_RS11130 0.82 +0.1 2,362,985 + ABZR87_RS11130 0.82 +0.2 2,362,985 + ABZR87_RS11130 0.82 +0.5 2,362,985 + ABZR87_RS11130 0.82 -1.8 2,362,985 + ABZR87_RS11130 0.82 -1.3 2,362,985 + ABZR87_RS11130 0.82 +1.6 2,362,985 + ABZR87_RS11130 0.82 -0.3 2,362,985 + ABZR87_RS11130 0.82 +0.6 2,362,985 + ABZR87_RS11130 0.82 -0.3 2,362,985 + ABZR87_RS11130 0.82 +0.7 2,362,985 + ABZR87_RS11130 0.82 -0.9 2,362,985 + ABZR87_RS11130 0.82 -0.7 2,362,985 + ABZR87_RS11130 0.82 -0.9 2,362,986 - ABZR87_RS11130 0.82 -0.5 2,362,986 - ABZR87_RS11130 0.82 -0.8 2,362,986 - ABZR87_RS11130 0.82 +0.2 2,362,986 - ABZR87_RS11130 0.82 -0.9 2,362,986 - ABZR87_RS11130 0.82 -2.2 2,362,986 - ABZR87_RS11130 0.82 -0.5 2,362,986 - ABZR87_RS11130 0.82 -0.5 2,362,986 - ABZR87_RS11130 0.82 -0.8 2,362,986 - ABZR87_RS11130 0.82 -1.3 2,362,986 - ABZR87_RS11130 0.82 -0.0 2,362,986 - ABZR87_RS11130 0.82 -0.8 2,362,986 - ABZR87_RS11130 0.82 +0.0 2,362,986 - ABZR87_RS11130 0.82 -0.2 2,363,113 + -0.1 2,363,113 + +0.2 2,363,113 + -1.2 2,363,113 + -1.0 2,363,114 - -0.4 2,363,172 + -0.2 2,363,172 + -0.4 2,363,175 - -0.0 2,363,175 - +1.2 2,363,175 - -0.3 2,363,175 - +0.8 2,363,248 - -0.4
Or see this region's nucleotide sequence