Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10345 and ABZR87_RS10350 overlap by 8 nucleotides ABZR87_RS10350 and ABZR87_RS10355 are separated by 98 nucleotides
ABZR87_RS10345: ABZR87_RS10345 - 3-isopropylmalate dehydratase large subunit, at 2,193,957 to 2,195,378
_RS10345
ABZR87_RS10350: ABZR87_RS10350 - 3-isopropylmalate dehydratase small subunit, at 2,195,371 to 2,196,033
_RS10350
ABZR87_RS10355: ABZR87_RS10355 - 2-hydroxycarboxylate transporter family protein, at 2,196,132 to 2,197,454
_RS10355
Position (kb)
2195
2196
2197 Strain fitness (log2 ratio)
-1
0
1
2 at 2194.468 kb on + strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.469 kb on - strand, within ABZR87_RS10345 at 2194.683 kb on + strand, within ABZR87_RS10345 at 2194.683 kb on + strand, within ABZR87_RS10345 at 2194.684 kb on - strand, within ABZR87_RS10345 at 2194.716 kb on - strand, within ABZR87_RS10345 at 2194.716 kb on - strand, within ABZR87_RS10345 at 2194.716 kb on - strand, within ABZR87_RS10345 at 2194.716 kb on - strand, within ABZR87_RS10345 at 2194.723 kb on + strand, within ABZR87_RS10345 at 2194.723 kb on + strand, within ABZR87_RS10345 at 2194.723 kb on + strand, within ABZR87_RS10345 at 2194.723 kb on + strand, within ABZR87_RS10345 at 2194.723 kb on + strand, within ABZR87_RS10345 at 2194.724 kb on - strand, within ABZR87_RS10345 at 2194.724 kb on - strand, within ABZR87_RS10345 at 2194.818 kb on + strand, within ABZR87_RS10345 at 2194.818 kb on + strand, within ABZR87_RS10345 at 2194.819 kb on - strand, within ABZR87_RS10345 at 2194.918 kb on - strand, within ABZR87_RS10345 at 2194.919 kb on + strand, within ABZR87_RS10345 at 2194.920 kb on - strand, within ABZR87_RS10345 at 2195.310 kb on - strand at 2195.392 kb on + strand at 2195.501 kb on - strand, within ABZR87_RS10350 at 2195.564 kb on - strand, within ABZR87_RS10350 at 2195.564 kb on - strand, within ABZR87_RS10350 at 2195.830 kb on - strand, within ABZR87_RS10350 at 2196.195 kb on + strand at 2196.226 kb on - strand at 2196.417 kb on + strand, within ABZR87_RS10355 at 2196.417 kb on + strand, within ABZR87_RS10355 at 2196.418 kb on - strand, within ABZR87_RS10355 at 2196.483 kb on + strand, within ABZR87_RS10355 at 2196.483 kb on + strand, within ABZR87_RS10355 at 2196.483 kb on + strand, within ABZR87_RS10355 at 2196.483 kb on + strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.484 kb on - strand, within ABZR87_RS10355 at 2196.489 kb on + strand, within ABZR87_RS10355 at 2196.489 kb on + strand, within ABZR87_RS10355 at 2196.489 kb on + strand, within ABZR87_RS10355 at 2196.489 kb on + strand, within ABZR87_RS10355 at 2196.489 kb on + strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.490 kb on - strand, within ABZR87_RS10355 at 2196.821 kb on + strand, within ABZR87_RS10355 at 2196.821 kb on + strand, within ABZR87_RS10355 at 2196.822 kb on - strand, within ABZR87_RS10355
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,194,468 + ABZR87_RS10345 0.36 +0.4 2,194,469 - ABZR87_RS10345 0.36 +1.4 2,194,469 - ABZR87_RS10345 0.36 +1.7 2,194,469 - ABZR87_RS10345 0.36 +0.6 2,194,469 - ABZR87_RS10345 0.36 -0.9 2,194,469 - ABZR87_RS10345 0.36 -1.5 2,194,469 - ABZR87_RS10345 0.36 -0.1 2,194,469 - ABZR87_RS10345 0.36 -1.0 2,194,683 + ABZR87_RS10345 0.51 -0.1 2,194,683 + ABZR87_RS10345 0.51 +0.3 2,194,684 - ABZR87_RS10345 0.51 -1.0 2,194,716 - ABZR87_RS10345 0.53 +2.0 2,194,716 - ABZR87_RS10345 0.53 +0.7 2,194,716 - ABZR87_RS10345 0.53 +1.3 2,194,716 - ABZR87_RS10345 0.53 -0.0 2,194,723 + ABZR87_RS10345 0.54 -0.7 2,194,723 + ABZR87_RS10345 0.54 +1.2 2,194,723 + ABZR87_RS10345 0.54 +0.9 2,194,723 + ABZR87_RS10345 0.54 +0.7 2,194,723 + ABZR87_RS10345 0.54 +0.0 2,194,724 - ABZR87_RS10345 0.54 -1.0 2,194,724 - ABZR87_RS10345 0.54 +0.1 2,194,818 + ABZR87_RS10345 0.61 +0.4 2,194,818 + ABZR87_RS10345 0.61 -1.6 2,194,819 - ABZR87_RS10345 0.61 +0.3 2,194,918 - ABZR87_RS10345 0.68 -0.5 2,194,919 + ABZR87_RS10345 0.68 +0.2 2,194,920 - ABZR87_RS10345 0.68 -0.9 2,195,310 - -0.4 2,195,392 + -1.0 2,195,501 - ABZR87_RS10350 0.20 -0.6 2,195,564 - ABZR87_RS10350 0.29 +0.8 2,195,564 - ABZR87_RS10350 0.29 -0.6 2,195,830 - ABZR87_RS10350 0.69 -1.2 2,196,195 + +0.2 2,196,226 - -0.6 2,196,417 + ABZR87_RS10355 0.22 -0.2 2,196,417 + ABZR87_RS10355 0.22 -1.1 2,196,418 - ABZR87_RS10355 0.22 +0.0 2,196,483 + ABZR87_RS10355 0.27 +0.7 2,196,483 + ABZR87_RS10355 0.27 -0.8 2,196,483 + ABZR87_RS10355 0.27 +0.2 2,196,483 + ABZR87_RS10355 0.27 -1.5 2,196,484 - ABZR87_RS10355 0.27 +1.2 2,196,484 - ABZR87_RS10355 0.27 +0.6 2,196,484 - ABZR87_RS10355 0.27 -0.3 2,196,484 - ABZR87_RS10355 0.27 -0.7 2,196,484 - ABZR87_RS10355 0.27 -0.9 2,196,484 - ABZR87_RS10355 0.27 +0.7 2,196,489 + ABZR87_RS10355 0.27 -1.4 2,196,489 + ABZR87_RS10355 0.27 -0.3 2,196,489 + ABZR87_RS10355 0.27 +0.4 2,196,489 + ABZR87_RS10355 0.27 -0.7 2,196,489 + ABZR87_RS10355 0.27 +1.0 2,196,490 - ABZR87_RS10355 0.27 -0.6 2,196,490 - ABZR87_RS10355 0.27 -1.0 2,196,490 - ABZR87_RS10355 0.27 +0.5 2,196,490 - ABZR87_RS10355 0.27 -0.6 2,196,490 - ABZR87_RS10355 0.27 -0.2 2,196,490 - ABZR87_RS10355 0.27 +0.2 2,196,490 - ABZR87_RS10355 0.27 -0.0 2,196,821 + ABZR87_RS10355 0.52 -0.0 2,196,821 + ABZR87_RS10355 0.52 -1.5 2,196,822 - ABZR87_RS10355 0.52 +0.0
Or see this region's nucleotide sequence