Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10180 and ABZR87_RS10185 overlap by 4 nucleotides ABZR87_RS10185 and ABZR87_RS10190 are separated by 216 nucleotides ABZR87_RS10190 and ABZR87_RS10195 are separated by 30 nucleotides ABZR87_RS10195 and ABZR87_RS10200 are separated by 62 nucleotides
ABZR87_RS10180: ABZR87_RS10180 - DegQ family serine endoprotease, at 2,159,948 to 2,161,459
_RS10180
ABZR87_RS10185: ABZR87_RS10185 - glutaredoxin family protein, at 2,161,456 to 2,161,731
_RS10185
ABZR87_RS10190: ABZR87_RS10190 - translation elongation factor 4, at 2,161,948 to 2,163,747
_RS10190
ABZR87_RS10195: ABZR87_RS10195 - signal peptidase I, at 2,163,778 to 2,164,671
_RS10195
ABZR87_RS10200: ABZR87_RS10200 - DUF4845 domain-containing protein, at 2,164,734 to 2,165,102
_RS10200
Position (kb)
2161
2162
2163
2164 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2160.956 kb on + strand, within ABZR87_RS10180 at 2160.957 kb on - strand, within ABZR87_RS10180 at 2160.957 kb on - strand, within ABZR87_RS10180 at 2160.970 kb on + strand, within ABZR87_RS10180 at 2161.138 kb on + strand, within ABZR87_RS10180 at 2161.139 kb on - strand, within ABZR87_RS10180 at 2161.139 kb on - strand, within ABZR87_RS10180 at 2161.390 kb on - strand at 2161.390 kb on - strand at 2161.467 kb on + strand at 2161.467 kb on + strand at 2161.468 kb on - strand at 2161.468 kb on - strand at 2161.468 kb on - strand at 2161.468 kb on - strand at 2161.486 kb on + strand, within ABZR87_RS10185 at 2161.486 kb on + strand, within ABZR87_RS10185 at 2161.486 kb on + strand, within ABZR87_RS10185 at 2161.487 kb on - strand, within ABZR87_RS10185 at 2161.487 kb on - strand, within ABZR87_RS10185 at 2161.487 kb on - strand, within ABZR87_RS10185 at 2161.487 kb on - strand, within ABZR87_RS10185 at 2161.607 kb on + strand, within ABZR87_RS10185 at 2161.607 kb on + strand, within ABZR87_RS10185 at 2161.607 kb on + strand, within ABZR87_RS10185 at 2161.607 kb on + strand, within ABZR87_RS10185 at 2161.608 kb on - strand, within ABZR87_RS10185 at 2161.608 kb on - strand, within ABZR87_RS10185 at 2161.608 kb on - strand, within ABZR87_RS10185 at 2161.673 kb on - strand, within ABZR87_RS10185 at 2161.685 kb on + strand, within ABZR87_RS10185 at 2161.685 kb on + strand, within ABZR87_RS10185 at 2161.795 kb on + strand at 2161.852 kb on + strand at 2161.852 kb on + strand at 2161.852 kb on + strand at 2161.852 kb on + strand at 2161.956 kb on + strand at 2161.956 kb on + strand at 2161.962 kb on + strand at 2162.134 kb on + strand, within ABZR87_RS10190 at 2162.134 kb on + strand, within ABZR87_RS10190 at 2162.134 kb on + strand, within ABZR87_RS10190 at 2162.135 kb on - strand, within ABZR87_RS10190 at 2162.135 kb on - strand, within ABZR87_RS10190 at 2162.136 kb on + strand, within ABZR87_RS10190 at 2162.136 kb on + strand, within ABZR87_RS10190 at 2162.136 kb on + strand, within ABZR87_RS10190 at 2162.136 kb on + strand, within ABZR87_RS10190 at 2162.137 kb on - strand, within ABZR87_RS10190 at 2162.137 kb on - strand, within ABZR87_RS10190 at 2162.158 kb on + strand, within ABZR87_RS10190 at 2162.158 kb on + strand, within ABZR87_RS10190 at 2162.159 kb on - strand, within ABZR87_RS10190 at 2162.203 kb on + strand, within ABZR87_RS10190 at 2162.203 kb on + strand, within ABZR87_RS10190 at 2162.204 kb on - strand, within ABZR87_RS10190 at 2162.297 kb on - strand, within ABZR87_RS10190 at 2162.554 kb on + strand, within ABZR87_RS10190 at 2162.554 kb on + strand, within ABZR87_RS10190 at 2162.555 kb on - strand, within ABZR87_RS10190 at 2162.555 kb on - strand, within ABZR87_RS10190 at 2162.555 kb on - strand, within ABZR87_RS10190 at 2162.555 kb on - strand, within ABZR87_RS10190 at 2162.869 kb on + strand, within ABZR87_RS10190 at 2162.870 kb on - strand, within ABZR87_RS10190 at 2162.927 kb on - strand, within ABZR87_RS10190 at 2163.042 kb on + strand, within ABZR87_RS10190 at 2163.043 kb on - strand, within ABZR87_RS10190 at 2163.065 kb on - strand, within ABZR87_RS10190 at 2163.065 kb on - strand, within ABZR87_RS10190 at 2163.178 kb on + strand, within ABZR87_RS10190 at 2163.178 kb on + strand, within ABZR87_RS10190 at 2163.178 kb on + strand, within ABZR87_RS10190 at 2163.178 kb on + strand, within ABZR87_RS10190 at 2163.178 kb on + strand, within ABZR87_RS10190 at 2163.179 kb on - strand, within ABZR87_RS10190 at 2163.179 kb on - strand, within ABZR87_RS10190 at 2163.253 kb on + strand, within ABZR87_RS10190 at 2163.253 kb on + strand, within ABZR87_RS10190 at 2163.253 kb on + strand, within ABZR87_RS10190 at 2163.281 kb on - strand, within ABZR87_RS10190 at 2163.346 kb on + strand, within ABZR87_RS10190 at 2163.358 kb on + strand, within ABZR87_RS10190 at 2163.361 kb on + strand, within ABZR87_RS10190 at 2163.470 kb on - strand, within ABZR87_RS10190 at 2163.523 kb on + strand, within ABZR87_RS10190 at 2163.523 kb on + strand, within ABZR87_RS10190 at 2163.625 kb on - strand at 2163.625 kb on - strand at 2163.925 kb on + strand, within ABZR87_RS10195 at 2164.003 kb on + strand, within ABZR87_RS10195 at 2164.141 kb on + strand, within ABZR87_RS10195 at 2164.330 kb on + strand, within ABZR87_RS10195 at 2164.444 kb on + strand, within ABZR87_RS10195 at 2164.490 kb on - strand, within ABZR87_RS10195 at 2164.531 kb on + strand, within ABZR87_RS10195
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,160,956 + ABZR87_RS10180 0.67 +0.4 2,160,957 - ABZR87_RS10180 0.67 -0.9 2,160,957 - ABZR87_RS10180 0.67 -0.7 2,160,970 + ABZR87_RS10180 0.68 -1.3 2,161,138 + ABZR87_RS10180 0.79 -0.4 2,161,139 - ABZR87_RS10180 0.79 +0.0 2,161,139 - ABZR87_RS10180 0.79 +0.2 2,161,390 - +0.2 2,161,390 - -1.9 2,161,467 + +0.6 2,161,467 + +2.4 2,161,468 - +0.4 2,161,468 - -0.6 2,161,468 - -0.7 2,161,468 - +0.1 2,161,486 + ABZR87_RS10185 0.11 +0.3 2,161,486 + ABZR87_RS10185 0.11 +1.0 2,161,486 + ABZR87_RS10185 0.11 -0.1 2,161,487 - ABZR87_RS10185 0.11 -0.6 2,161,487 - ABZR87_RS10185 0.11 +1.1 2,161,487 - ABZR87_RS10185 0.11 +0.0 2,161,487 - ABZR87_RS10185 0.11 -0.9 2,161,607 + ABZR87_RS10185 0.55 +0.4 2,161,607 + ABZR87_RS10185 0.55 -1.0 2,161,607 + ABZR87_RS10185 0.55 -0.8 2,161,607 + ABZR87_RS10185 0.55 +0.6 2,161,608 - ABZR87_RS10185 0.55 +0.4 2,161,608 - ABZR87_RS10185 0.55 +0.4 2,161,608 - ABZR87_RS10185 0.55 -0.3 2,161,673 - ABZR87_RS10185 0.79 +0.2 2,161,685 + ABZR87_RS10185 0.83 +0.3 2,161,685 + ABZR87_RS10185 0.83 -1.0 2,161,795 + +0.7 2,161,852 + -0.6 2,161,852 + -0.3 2,161,852 + -0.3 2,161,852 + +0.3 2,161,956 + -1.0 2,161,956 + -0.5 2,161,962 + -0.3 2,162,134 + ABZR87_RS10190 0.10 +0.6 2,162,134 + ABZR87_RS10190 0.10 -1.5 2,162,134 + ABZR87_RS10190 0.10 -0.1 2,162,135 - ABZR87_RS10190 0.10 -1.5 2,162,135 - ABZR87_RS10190 0.10 -1.9 2,162,136 + ABZR87_RS10190 0.10 -1.9 2,162,136 + ABZR87_RS10190 0.10 -1.5 2,162,136 + ABZR87_RS10190 0.10 -2.7 2,162,136 + ABZR87_RS10190 0.10 -0.8 2,162,137 - ABZR87_RS10190 0.10 -1.0 2,162,137 - ABZR87_RS10190 0.10 -0.9 2,162,158 + ABZR87_RS10190 0.12 -1.3 2,162,158 + ABZR87_RS10190 0.12 -1.2 2,162,159 - ABZR87_RS10190 0.12 +1.0 2,162,203 + ABZR87_RS10190 0.14 -0.4 2,162,203 + ABZR87_RS10190 0.14 +0.5 2,162,204 - ABZR87_RS10190 0.14 -0.0 2,162,297 - ABZR87_RS10190 0.19 -0.0 2,162,554 + ABZR87_RS10190 0.34 -0.8 2,162,554 + ABZR87_RS10190 0.34 +1.0 2,162,555 - ABZR87_RS10190 0.34 -0.2 2,162,555 - ABZR87_RS10190 0.34 -0.3 2,162,555 - ABZR87_RS10190 0.34 +0.4 2,162,555 - ABZR87_RS10190 0.34 -1.5 2,162,869 + ABZR87_RS10190 0.51 -0.0 2,162,870 - ABZR87_RS10190 0.51 -0.2 2,162,927 - ABZR87_RS10190 0.54 -0.3 2,163,042 + ABZR87_RS10190 0.61 -1.7 2,163,043 - ABZR87_RS10190 0.61 -1.0 2,163,065 - ABZR87_RS10190 0.62 +0.7 2,163,065 - ABZR87_RS10190 0.62 -1.0 2,163,178 + ABZR87_RS10190 0.68 -0.7 2,163,178 + ABZR87_RS10190 0.68 -0.5 2,163,178 + ABZR87_RS10190 0.68 -0.3 2,163,178 + ABZR87_RS10190 0.68 -1.2 2,163,178 + ABZR87_RS10190 0.68 +0.4 2,163,179 - ABZR87_RS10190 0.68 -1.2 2,163,179 - ABZR87_RS10190 0.68 +0.4 2,163,253 + ABZR87_RS10190 0.72 +2.8 2,163,253 + ABZR87_RS10190 0.72 +0.6 2,163,253 + ABZR87_RS10190 0.72 -0.8 2,163,281 - ABZR87_RS10190 0.74 +0.4 2,163,346 + ABZR87_RS10190 0.78 -0.2 2,163,358 + ABZR87_RS10190 0.78 +1.4 2,163,361 + ABZR87_RS10190 0.79 -0.5 2,163,470 - ABZR87_RS10190 0.85 +0.4 2,163,523 + ABZR87_RS10190 0.88 +0.2 2,163,523 + ABZR87_RS10190 0.88 -0.5 2,163,625 - -1.3 2,163,625 - +0.8 2,163,925 + ABZR87_RS10195 0.16 +1.2 2,164,003 + ABZR87_RS10195 0.25 -0.0 2,164,141 + ABZR87_RS10195 0.41 +2.0 2,164,330 + ABZR87_RS10195 0.62 -0.3 2,164,444 + ABZR87_RS10195 0.74 +0.8 2,164,490 - ABZR87_RS10195 0.80 -0.8 2,164,531 + ABZR87_RS10195 0.84 +1.0
Or see this region's nucleotide sequence