Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10155 and ABZR87_RS10160 are separated by 54 nucleotides ABZR87_RS10160 and ABZR87_RS10165 are separated by 335 nucleotides ABZR87_RS10165 and ABZR87_RS10170 are separated by 107 nucleotides ABZR87_RS10170 and ABZR87_RS10175 are separated by 11 nucleotides
ABZR87_RS10155: ABZR87_RS10155 - beta-ketoacyl-ACP synthase II, at 2,155,582 to 2,156,814
_RS10155
ABZR87_RS10160: ABZR87_RS10160 - hypothetical protein, at 2,156,869 to 2,157,078
_RS10160
ABZR87_RS10165: ABZR87_RS10165 - RNA polymerase sigma factor RpoE, at 2,157,414 to 2,158,013
_RS10165
ABZR87_RS10170: ABZR87_RS10170 - RseA family anti-sigma factor, at 2,158,121 to 2,158,753
_RS10170
ABZR87_RS10175: ABZR87_RS10175 - MucB/RseB C-terminal domain-containing protein, at 2,158,765 to 2,159,862
_RS10175
Position (kb)
2157
2158
2159 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2156.817 kb on + strand at 2156.830 kb on + strand at 2156.830 kb on + strand at 2156.830 kb on + strand at 2156.831 kb on - strand at 2156.831 kb on - strand at 2156.831 kb on - strand at 2156.955 kb on + strand, within ABZR87_RS10160 at 2156.956 kb on - strand, within ABZR87_RS10160 at 2157.034 kb on + strand, within ABZR87_RS10160 at 2157.034 kb on + strand, within ABZR87_RS10160 at 2157.034 kb on + strand, within ABZR87_RS10160 at 2157.034 kb on + strand, within ABZR87_RS10160 at 2157.035 kb on - strand, within ABZR87_RS10160 at 2157.174 kb on + strand at 2157.174 kb on + strand at 2157.175 kb on - strand at 2157.175 kb on - strand at 2157.175 kb on - strand at 2157.175 kb on - strand at 2157.282 kb on + strand at 2157.282 kb on + strand at 2157.283 kb on - strand at 2157.283 kb on - strand at 2157.283 kb on - strand at 2157.289 kb on + strand at 2157.290 kb on - strand at 2157.290 kb on - strand at 2157.290 kb on - strand at 2157.290 kb on - strand at 2157.290 kb on - strand at 2157.350 kb on + strand at 2157.351 kb on - strand at 2157.351 kb on - strand at 2157.359 kb on + strand at 2157.359 kb on + strand at 2157.360 kb on - strand at 2157.360 kb on - strand at 2157.393 kb on + strand at 2157.467 kb on + strand at 2157.467 kb on + strand at 2157.498 kb on + strand, within ABZR87_RS10165 at 2157.498 kb on + strand, within ABZR87_RS10165 at 2157.498 kb on + strand, within ABZR87_RS10165 at 2157.498 kb on + strand, within ABZR87_RS10165 at 2157.499 kb on - strand, within ABZR87_RS10165 at 2157.578 kb on + strand, within ABZR87_RS10165 at 2157.578 kb on + strand, within ABZR87_RS10165 at 2157.579 kb on - strand, within ABZR87_RS10165 at 2157.579 kb on - strand, within ABZR87_RS10165 at 2157.579 kb on - strand, within ABZR87_RS10165 at 2157.579 kb on - strand, within ABZR87_RS10165 at 2157.598 kb on + strand, within ABZR87_RS10165 at 2157.627 kb on + strand, within ABZR87_RS10165 at 2157.628 kb on - strand, within ABZR87_RS10165 at 2157.628 kb on - strand, within ABZR87_RS10165 at 2157.628 kb on - strand, within ABZR87_RS10165 at 2157.628 kb on - strand, within ABZR87_RS10165 at 2157.628 kb on - strand, within ABZR87_RS10165 at 2157.628 kb on - strand, within ABZR87_RS10165 at 2157.629 kb on + strand, within ABZR87_RS10165 at 2157.629 kb on + strand, within ABZR87_RS10165 at 2157.629 kb on + strand, within ABZR87_RS10165 at 2157.629 kb on + strand, within ABZR87_RS10165 at 2157.629 kb on + strand, within ABZR87_RS10165 at 2157.629 kb on + strand, within ABZR87_RS10165 at 2157.629 kb on + strand, within ABZR87_RS10165 at 2157.630 kb on - strand, within ABZR87_RS10165 at 2157.630 kb on - strand, within ABZR87_RS10165 at 2157.630 kb on - strand, within ABZR87_RS10165 at 2157.630 kb on - strand, within ABZR87_RS10165 at 2157.630 kb on - strand, within ABZR87_RS10165 at 2157.640 kb on - strand, within ABZR87_RS10165 at 2157.656 kb on + strand, within ABZR87_RS10165 at 2157.657 kb on - strand, within ABZR87_RS10165 at 2157.669 kb on + strand, within ABZR87_RS10165 at 2157.669 kb on + strand, within ABZR87_RS10165 at 2157.751 kb on + strand, within ABZR87_RS10165 at 2157.751 kb on + strand, within ABZR87_RS10165 at 2157.751 kb on + strand, within ABZR87_RS10165 at 2157.751 kb on + strand, within ABZR87_RS10165 at 2157.752 kb on - strand, within ABZR87_RS10165 at 2157.754 kb on + strand, within ABZR87_RS10165 at 2157.754 kb on + strand, within ABZR87_RS10165 at 2157.767 kb on + strand, within ABZR87_RS10165 at 2157.767 kb on + strand, within ABZR87_RS10165 at 2157.768 kb on - strand, within ABZR87_RS10165 at 2157.768 kb on - strand, within ABZR87_RS10165 at 2157.768 kb on - strand, within ABZR87_RS10165 at 2157.885 kb on + strand, within ABZR87_RS10165 at 2157.885 kb on + strand, within ABZR87_RS10165 at 2157.885 kb on + strand, within ABZR87_RS10165 at 2157.885 kb on + strand, within ABZR87_RS10165 at 2157.886 kb on - strand, within ABZR87_RS10165 at 2157.886 kb on - strand, within ABZR87_RS10165 at 2157.886 kb on - strand, within ABZR87_RS10165 at 2157.886 kb on - strand, within ABZR87_RS10165 at 2158.309 kb on + strand, within ABZR87_RS10170 at 2158.309 kb on + strand, within ABZR87_RS10170 at 2158.309 kb on + strand, within ABZR87_RS10170 at 2158.310 kb on - strand, within ABZR87_RS10170 at 2158.310 kb on - strand, within ABZR87_RS10170 at 2158.310 kb on - strand, within ABZR87_RS10170 at 2158.492 kb on + strand, within ABZR87_RS10170 at 2158.493 kb on - strand, within ABZR87_RS10170 at 2158.650 kb on - strand, within ABZR87_RS10170 at 2158.650 kb on - strand, within ABZR87_RS10170 at 2158.684 kb on + strand, within ABZR87_RS10170 at 2158.684 kb on + strand, within ABZR87_RS10170 at 2158.685 kb on - strand, within ABZR87_RS10170 at 2158.698 kb on - strand at 2158.752 kb on - strand at 2158.954 kb on + strand, within ABZR87_RS10175 at 2158.954 kb on + strand, within ABZR87_RS10175 at 2158.955 kb on - strand, within ABZR87_RS10175 at 2158.955 kb on - strand, within ABZR87_RS10175 at 2158.972 kb on + strand, within ABZR87_RS10175 at 2158.972 kb on + strand, within ABZR87_RS10175 at 2158.973 kb on - strand, within ABZR87_RS10175
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,156,817 + +0.6 2,156,830 + +1.3 2,156,830 + -1.8 2,156,830 + -0.2 2,156,831 - -1.2 2,156,831 - -1.2 2,156,831 - +0.4 2,156,955 + ABZR87_RS10160 0.41 -1.2 2,156,956 - ABZR87_RS10160 0.41 +0.0 2,157,034 + ABZR87_RS10160 0.79 +0.2 2,157,034 + ABZR87_RS10160 0.79 -0.3 2,157,034 + ABZR87_RS10160 0.79 +0.1 2,157,034 + ABZR87_RS10160 0.79 -1.0 2,157,035 - ABZR87_RS10160 0.79 +1.5 2,157,174 + -0.4 2,157,174 + -0.3 2,157,175 - +1.6 2,157,175 - +1.5 2,157,175 - +1.2 2,157,175 - +1.9 2,157,282 + -0.6 2,157,282 + -0.6 2,157,283 - +2.1 2,157,283 - +1.3 2,157,283 - +2.0 2,157,289 + -0.2 2,157,290 - +2.4 2,157,290 - +1.6 2,157,290 - +2.4 2,157,290 - +1.3 2,157,290 - +1.7 2,157,350 + +0.8 2,157,351 - +1.3 2,157,351 - +1.4 2,157,359 + -1.5 2,157,359 + -1.5 2,157,360 - +1.9 2,157,360 - +1.6 2,157,393 + +2.4 2,157,467 + +3.1 2,157,467 + +2.5 2,157,498 + ABZR87_RS10165 0.14 +2.4 2,157,498 + ABZR87_RS10165 0.14 +2.7 2,157,498 + ABZR87_RS10165 0.14 +2.7 2,157,498 + ABZR87_RS10165 0.14 +3.2 2,157,499 - ABZR87_RS10165 0.14 +2.3 2,157,578 + ABZR87_RS10165 0.27 +2.3 2,157,578 + ABZR87_RS10165 0.27 +2.9 2,157,579 - ABZR87_RS10165 0.28 +2.6 2,157,579 - ABZR87_RS10165 0.28 +3.2 2,157,579 - ABZR87_RS10165 0.28 +2.1 2,157,579 - ABZR87_RS10165 0.28 +2.8 2,157,598 + ABZR87_RS10165 0.31 +2.5 2,157,627 + ABZR87_RS10165 0.35 +2.5 2,157,628 - ABZR87_RS10165 0.36 +2.5 2,157,628 - ABZR87_RS10165 0.36 +3.2 2,157,628 - ABZR87_RS10165 0.36 +3.1 2,157,628 - ABZR87_RS10165 0.36 +2.4 2,157,628 - ABZR87_RS10165 0.36 +2.7 2,157,628 - ABZR87_RS10165 0.36 +1.3 2,157,629 + ABZR87_RS10165 0.36 +2.6 2,157,629 + ABZR87_RS10165 0.36 +2.3 2,157,629 + ABZR87_RS10165 0.36 +2.3 2,157,629 + ABZR87_RS10165 0.36 +3.3 2,157,629 + ABZR87_RS10165 0.36 +2.2 2,157,629 + ABZR87_RS10165 0.36 +2.1 2,157,629 + ABZR87_RS10165 0.36 +2.4 2,157,630 - ABZR87_RS10165 0.36 +2.7 2,157,630 - ABZR87_RS10165 0.36 +2.0 2,157,630 - ABZR87_RS10165 0.36 +3.0 2,157,630 - ABZR87_RS10165 0.36 +2.9 2,157,630 - ABZR87_RS10165 0.36 +2.5 2,157,640 - ABZR87_RS10165 0.38 +2.3 2,157,656 + ABZR87_RS10165 0.40 +2.2 2,157,657 - ABZR87_RS10165 0.41 +2.3 2,157,669 + ABZR87_RS10165 0.42 +2.4 2,157,669 + ABZR87_RS10165 0.42 +2.2 2,157,751 + ABZR87_RS10165 0.56 +3.1 2,157,751 + ABZR87_RS10165 0.56 +2.7 2,157,751 + ABZR87_RS10165 0.56 +2.5 2,157,751 + ABZR87_RS10165 0.56 +2.6 2,157,752 - ABZR87_RS10165 0.56 +2.8 2,157,754 + ABZR87_RS10165 0.57 +2.3 2,157,754 + ABZR87_RS10165 0.57 +2.3 2,157,767 + ABZR87_RS10165 0.59 +2.8 2,157,767 + ABZR87_RS10165 0.59 +2.6 2,157,768 - ABZR87_RS10165 0.59 +2.2 2,157,768 - ABZR87_RS10165 0.59 +2.8 2,157,768 - ABZR87_RS10165 0.59 +3.0 2,157,885 + ABZR87_RS10165 0.79 +3.0 2,157,885 + ABZR87_RS10165 0.79 +2.3 2,157,885 + ABZR87_RS10165 0.79 +2.5 2,157,885 + ABZR87_RS10165 0.79 +2.5 2,157,886 - ABZR87_RS10165 0.79 +2.8 2,157,886 - ABZR87_RS10165 0.79 +2.6 2,157,886 - ABZR87_RS10165 0.79 +2.4 2,157,886 - ABZR87_RS10165 0.79 +2.6 2,158,309 + ABZR87_RS10170 0.30 -1.9 2,158,309 + ABZR87_RS10170 0.30 -1.1 2,158,309 + ABZR87_RS10170 0.30 +0.4 2,158,310 - ABZR87_RS10170 0.30 -1.3 2,158,310 - ABZR87_RS10170 0.30 -0.3 2,158,310 - ABZR87_RS10170 0.30 -0.6 2,158,492 + ABZR87_RS10170 0.59 +0.8 2,158,493 - ABZR87_RS10170 0.59 -0.3 2,158,650 - ABZR87_RS10170 0.84 -1.9 2,158,650 - ABZR87_RS10170 0.84 +0.8 2,158,684 + ABZR87_RS10170 0.89 -1.0 2,158,684 + ABZR87_RS10170 0.89 -0.3 2,158,685 - ABZR87_RS10170 0.89 -0.2 2,158,698 - -0.9 2,158,752 - -1.0 2,158,954 + ABZR87_RS10175 0.17 -1.3 2,158,954 + ABZR87_RS10175 0.17 -2.6 2,158,955 - ABZR87_RS10175 0.17 -2.3 2,158,955 - ABZR87_RS10175 0.17 -0.6 2,158,972 + ABZR87_RS10175 0.19 +0.4 2,158,972 + ABZR87_RS10175 0.19 +0.8 2,158,973 - ABZR87_RS10175 0.19 +1.1
Or see this region's nucleotide sequence