Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10110 and ABZR87_RS10115 are separated by 3 nucleotides ABZR87_RS10115 and ABZR87_RS10120 are separated by 190 nucleotides ABZR87_RS10120 and ABZR87_RS10125 are separated by 115 nucleotides ABZR87_RS10125 and ABZR87_RS10130 are separated by 190 nucleotides
ABZR87_RS10110: ABZR87_RS10110 - SAM-dependent methyltransferase, at 2,148,694 to 2,149,425
_RS10110
ABZR87_RS10115: ABZR87_RS10115 - Maf-like protein, at 2,149,429 to 2,150,025
_RS10115
ABZR87_RS10120: ABZR87_RS10120 - YceD family protein, at 2,150,216 to 2,150,752
_RS10120
ABZR87_RS10125: ABZR87_RS10125 - 50S ribosomal protein L32, at 2,150,868 to 2,151,047
_RS10125
ABZR87_RS10130: ABZR87_RS10130 - phosphate acyltransferase PlsX, at 2,151,238 to 2,152,299
_RS10130
Position (kb)
2150
2151 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2149.276 kb on + strand, within ABZR87_RS10110 at 2149.314 kb on - strand, within ABZR87_RS10110 at 2149.314 kb on - strand, within ABZR87_RS10110 at 2149.390 kb on + strand at 2149.390 kb on + strand at 2149.390 kb on + strand at 2149.390 kb on + strand at 2149.390 kb on + strand at 2149.391 kb on - strand at 2149.391 kb on - strand at 2149.391 kb on - strand at 2149.395 kb on + strand at 2149.395 kb on + strand at 2149.396 kb on - strand at 2149.409 kb on + strand at 2149.409 kb on + strand at 2149.409 kb on + strand at 2149.410 kb on - strand at 2149.410 kb on - strand at 2149.416 kb on + strand at 2149.416 kb on + strand at 2149.417 kb on - strand at 2150.025 kb on - strand at 2150.026 kb on - strand at 2150.026 kb on - strand at 2150.088 kb on + strand at 2150.088 kb on + strand at 2150.089 kb on - strand at 2150.204 kb on + strand at 2150.204 kb on + strand at 2150.204 kb on + strand at 2150.205 kb on - strand at 2150.344 kb on + strand, within ABZR87_RS10120 at 2150.481 kb on - strand, within ABZR87_RS10120 at 2150.481 kb on - strand, within ABZR87_RS10120 at 2150.764 kb on + strand at 2150.764 kb on + strand at 2150.785 kb on + strand at 2150.785 kb on + strand at 2151.171 kb on + strand at 2151.171 kb on + strand at 2151.184 kb on - strand at 2151.184 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,149,276 + ABZR87_RS10110 0.80 -0.4 2,149,314 - ABZR87_RS10110 0.85 -0.4 2,149,314 - ABZR87_RS10110 0.85 -0.9 2,149,390 + -1.0 2,149,390 + -1.4 2,149,390 + -0.4 2,149,390 + +0.1 2,149,390 + -0.2 2,149,391 - -0.4 2,149,391 - +0.7 2,149,391 - -0.2 2,149,395 + -0.1 2,149,395 + +0.3 2,149,396 - -1.0 2,149,409 + -0.7 2,149,409 + -0.1 2,149,409 + +0.2 2,149,410 - -0.5 2,149,410 - -0.3 2,149,416 + -0.4 2,149,416 + +1.3 2,149,417 - -0.1 2,150,025 - -2.1 2,150,026 - -1.7 2,150,026 - -1.4 2,150,088 + -1.9 2,150,088 + -0.2 2,150,089 - +2.5 2,150,204 + +0.6 2,150,204 + +0.0 2,150,204 + -0.5 2,150,205 - -0.7 2,150,344 + ABZR87_RS10120 0.24 -0.1 2,150,481 - ABZR87_RS10120 0.49 -1.3 2,150,481 - ABZR87_RS10120 0.49 -1.9 2,150,764 + -0.6 2,150,764 + -0.9 2,150,785 + -0.6 2,150,785 + +0.2 2,151,171 + -2.2 2,151,171 + +0.7 2,151,184 - -1.9 2,151,184 - +0.1
Or see this region's nucleotide sequence