Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS09310 and ABZR87_RS09315 are separated by 7 nucleotides ABZR87_RS09315 and ABZR87_RS09320 are separated by 10 nucleotides ABZR87_RS09320 and ABZR87_RS09325 are separated by 75 nucleotides
ABZR87_RS09310: ABZR87_RS09310 - TonB-dependent siderophore receptor, at 1,972,315 to 1,974,423
_RS09310
ABZR87_RS09315: ABZR87_RS09315 - slipin family protein, at 1,974,431 to 1,975,189
_RS09315
ABZR87_RS09320: ABZR87_RS09320 - nodulation protein NfeD, at 1,975,200 to 1,976,579
_RS09320
ABZR87_RS09325: ABZR87_RS09325 - LysR family transcriptional regulator, at 1,976,655 to 1,977,563
_RS09325
Position (kb)
1975
1976
1977 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1974.275 kb on - strand at 1974.275 kb on - strand at 1974.464 kb on + strand at 1974.464 kb on + strand at 1974.465 kb on - strand at 1974.465 kb on - strand at 1974.465 kb on - strand at 1974.534 kb on + strand, within ABZR87_RS09315 at 1974.588 kb on - strand, within ABZR87_RS09315 at 1974.921 kb on + strand, within ABZR87_RS09315 at 1974.922 kb on - strand, within ABZR87_RS09315 at 1974.947 kb on + strand, within ABZR87_RS09315 at 1974.947 kb on + strand, within ABZR87_RS09315 at 1974.947 kb on + strand, within ABZR87_RS09315 at 1974.948 kb on - strand, within ABZR87_RS09315 at 1974.948 kb on - strand, within ABZR87_RS09315 at 1974.948 kb on - strand, within ABZR87_RS09315 at 1974.948 kb on - strand, within ABZR87_RS09315 at 1975.007 kb on + strand, within ABZR87_RS09315 at 1975.007 kb on + strand, within ABZR87_RS09315 at 1975.007 kb on + strand, within ABZR87_RS09315 at 1975.008 kb on - strand, within ABZR87_RS09315 at 1975.008 kb on - strand, within ABZR87_RS09315 at 1975.104 kb on + strand, within ABZR87_RS09315 at 1975.182 kb on + strand at 1975.182 kb on + strand at 1975.183 kb on - strand at 1975.524 kb on - strand, within ABZR87_RS09320 at 1975.651 kb on + strand, within ABZR87_RS09320 at 1975.652 kb on - strand, within ABZR87_RS09320 at 1975.652 kb on - strand, within ABZR87_RS09320 at 1975.652 kb on - strand, within ABZR87_RS09320 at 1975.679 kb on - strand, within ABZR87_RS09320 at 1975.760 kb on - strand, within ABZR87_RS09320 at 1975.760 kb on - strand, within ABZR87_RS09320 at 1976.062 kb on + strand, within ABZR87_RS09320 at 1976.062 kb on + strand, within ABZR87_RS09320 at 1976.062 kb on + strand, within ABZR87_RS09320 at 1976.062 kb on + strand, within ABZR87_RS09320 at 1976.062 kb on + strand, within ABZR87_RS09320 at 1976.063 kb on - strand, within ABZR87_RS09320 at 1976.063 kb on - strand, within ABZR87_RS09320 at 1976.063 kb on - strand, within ABZR87_RS09320 at 1976.063 kb on - strand, within ABZR87_RS09320 at 1976.063 kb on - strand, within ABZR87_RS09320 at 1976.245 kb on + strand, within ABZR87_RS09320 at 1976.245 kb on + strand, within ABZR87_RS09320 at 1976.245 kb on + strand, within ABZR87_RS09320 at 1976.245 kb on + strand, within ABZR87_RS09320 at 1976.246 kb on - strand, within ABZR87_RS09320 at 1976.246 kb on - strand, within ABZR87_RS09320 at 1976.254 kb on + strand, within ABZR87_RS09320 at 1976.254 kb on - strand, within ABZR87_RS09320 at 1976.255 kb on - strand, within ABZR87_RS09320 at 1976.255 kb on - strand, within ABZR87_RS09320 at 1976.255 kb on - strand, within ABZR87_RS09320 at 1976.255 kb on - strand, within ABZR87_RS09320 at 1976.255 kb on - strand, within ABZR87_RS09320 at 1976.296 kb on + strand, within ABZR87_RS09320 at 1976.296 kb on + strand, within ABZR87_RS09320 at 1976.297 kb on - strand, within ABZR87_RS09320 at 1976.297 kb on - strand, within ABZR87_RS09320 at 1976.372 kb on + strand, within ABZR87_RS09320 at 1976.373 kb on - strand, within ABZR87_RS09320 at 1976.438 kb on - strand, within ABZR87_RS09320 at 1976.438 kb on - strand, within ABZR87_RS09320 at 1976.642 kb on - strand at 1976.657 kb on - strand at 1976.825 kb on + strand, within ABZR87_RS09325 at 1976.825 kb on + strand, within ABZR87_RS09325 at 1976.825 kb on + strand, within ABZR87_RS09325 at 1976.825 kb on + strand, within ABZR87_RS09325 at 1976.826 kb on - strand, within ABZR87_RS09325 at 1976.826 kb on - strand, within ABZR87_RS09325 at 1976.826 kb on - strand, within ABZR87_RS09325 at 1976.828 kb on - strand, within ABZR87_RS09325 at 1976.853 kb on + strand, within ABZR87_RS09325 at 1976.899 kb on + strand, within ABZR87_RS09325 at 1976.899 kb on + strand, within ABZR87_RS09325 at 1976.899 kb on + strand, within ABZR87_RS09325 at 1976.899 kb on + strand, within ABZR87_RS09325 at 1976.900 kb on - strand, within ABZR87_RS09325 at 1977.012 kb on + strand, within ABZR87_RS09325 at 1977.211 kb on - strand, within ABZR87_RS09325 at 1977.211 kb on - strand, within ABZR87_RS09325 at 1977.211 kb on - strand, within ABZR87_RS09325 at 1977.287 kb on + strand, within ABZR87_RS09325 at 1977.287 kb on + strand, within ABZR87_RS09325 at 1977.288 kb on - strand, within ABZR87_RS09325 at 1977.364 kb on + strand, within ABZR87_RS09325 at 1977.365 kb on - strand, within ABZR87_RS09325 at 1977.365 kb on - strand, within ABZR87_RS09325 at 1977.365 kb on - strand, within ABZR87_RS09325 at 1977.568 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,974,275 - -1.3 1,974,275 - -0.7 1,974,464 + -0.3 1,974,464 + -0.2 1,974,465 - -0.8 1,974,465 - -0.0 1,974,465 - -0.5 1,974,534 + ABZR87_RS09315 0.14 -2.3 1,974,588 - ABZR87_RS09315 0.21 -0.1 1,974,921 + ABZR87_RS09315 0.65 -1.0 1,974,922 - ABZR87_RS09315 0.65 +0.6 1,974,947 + ABZR87_RS09315 0.68 -0.4 1,974,947 + ABZR87_RS09315 0.68 -0.1 1,974,947 + ABZR87_RS09315 0.68 -0.6 1,974,948 - ABZR87_RS09315 0.68 -0.4 1,974,948 - ABZR87_RS09315 0.68 -1.8 1,974,948 - ABZR87_RS09315 0.68 +0.0 1,974,948 - ABZR87_RS09315 0.68 +1.9 1,975,007 + ABZR87_RS09315 0.76 -0.5 1,975,007 + ABZR87_RS09315 0.76 -0.9 1,975,007 + ABZR87_RS09315 0.76 +0.4 1,975,008 - ABZR87_RS09315 0.76 +0.8 1,975,008 - ABZR87_RS09315 0.76 +1.2 1,975,104 + ABZR87_RS09315 0.89 -1.5 1,975,182 + -0.9 1,975,182 + +0.0 1,975,183 - +0.6 1,975,524 - ABZR87_RS09320 0.23 -0.2 1,975,651 + ABZR87_RS09320 0.33 +0.2 1,975,652 - ABZR87_RS09320 0.33 -1.0 1,975,652 - ABZR87_RS09320 0.33 -2.0 1,975,652 - ABZR87_RS09320 0.33 -0.5 1,975,679 - ABZR87_RS09320 0.35 -0.4 1,975,760 - ABZR87_RS09320 0.41 -0.0 1,975,760 - ABZR87_RS09320 0.41 +0.1 1,976,062 + ABZR87_RS09320 0.62 -0.9 1,976,062 + ABZR87_RS09320 0.62 +0.4 1,976,062 + ABZR87_RS09320 0.62 -0.1 1,976,062 + ABZR87_RS09320 0.62 -0.1 1,976,062 + ABZR87_RS09320 0.62 -0.7 1,976,063 - ABZR87_RS09320 0.63 -1.1 1,976,063 - ABZR87_RS09320 0.63 -0.4 1,976,063 - ABZR87_RS09320 0.63 -1.2 1,976,063 - ABZR87_RS09320 0.63 -0.6 1,976,063 - ABZR87_RS09320 0.63 -0.6 1,976,245 + ABZR87_RS09320 0.76 -1.4 1,976,245 + ABZR87_RS09320 0.76 -0.6 1,976,245 + ABZR87_RS09320 0.76 +0.0 1,976,245 + ABZR87_RS09320 0.76 +0.1 1,976,246 - ABZR87_RS09320 0.76 +1.2 1,976,246 - ABZR87_RS09320 0.76 -0.0 1,976,254 + ABZR87_RS09320 0.76 +0.3 1,976,254 - ABZR87_RS09320 0.76 -0.7 1,976,255 - ABZR87_RS09320 0.76 +1.1 1,976,255 - ABZR87_RS09320 0.76 -0.1 1,976,255 - ABZR87_RS09320 0.76 +0.1 1,976,255 - ABZR87_RS09320 0.76 -1.1 1,976,255 - ABZR87_RS09320 0.76 -0.7 1,976,296 + ABZR87_RS09320 0.79 -0.6 1,976,296 + ABZR87_RS09320 0.79 -0.8 1,976,297 - ABZR87_RS09320 0.79 +2.5 1,976,297 - ABZR87_RS09320 0.79 -2.0 1,976,372 + ABZR87_RS09320 0.85 -0.4 1,976,373 - ABZR87_RS09320 0.85 -0.9 1,976,438 - ABZR87_RS09320 0.90 +0.9 1,976,438 - ABZR87_RS09320 0.90 +0.6 1,976,642 - -0.4 1,976,657 - +0.4 1,976,825 + ABZR87_RS09325 0.19 -0.4 1,976,825 + ABZR87_RS09325 0.19 -1.4 1,976,825 + ABZR87_RS09325 0.19 +0.3 1,976,825 + ABZR87_RS09325 0.19 +0.3 1,976,826 - ABZR87_RS09325 0.19 +0.6 1,976,826 - ABZR87_RS09325 0.19 +0.3 1,976,826 - ABZR87_RS09325 0.19 -0.8 1,976,828 - ABZR87_RS09325 0.19 +0.3 1,976,853 + ABZR87_RS09325 0.22 -0.3 1,976,899 + ABZR87_RS09325 0.27 +0.3 1,976,899 + ABZR87_RS09325 0.27 +0.1 1,976,899 + ABZR87_RS09325 0.27 -1.0 1,976,899 + ABZR87_RS09325 0.27 +1.6 1,976,900 - ABZR87_RS09325 0.27 -0.4 1,977,012 + ABZR87_RS09325 0.39 -0.6 1,977,211 - ABZR87_RS09325 0.61 -1.2 1,977,211 - ABZR87_RS09325 0.61 +1.6 1,977,211 - ABZR87_RS09325 0.61 -0.1 1,977,287 + ABZR87_RS09325 0.70 -0.1 1,977,287 + ABZR87_RS09325 0.70 -0.2 1,977,288 - ABZR87_RS09325 0.70 +0.8 1,977,364 + ABZR87_RS09325 0.78 -0.0 1,977,365 - ABZR87_RS09325 0.78 +2.2 1,977,365 - ABZR87_RS09325 0.78 -0.9 1,977,365 - ABZR87_RS09325 0.78 -0.4 1,977,568 + -0.1
Or see this region's nucleotide sequence