Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS09310 and ABZR87_RS09315 are separated by 7 nucleotides ABZR87_RS09315 and ABZR87_RS09320 are separated by 10 nucleotides
ABZR87_RS09310: ABZR87_RS09310 - TonB-dependent siderophore receptor, at 1,972,315 to 1,974,423
_RS09310
ABZR87_RS09315: ABZR87_RS09315 - slipin family protein, at 1,974,431 to 1,975,189
_RS09315
ABZR87_RS09320: ABZR87_RS09320 - nodulation protein NfeD, at 1,975,200 to 1,976,579
_RS09320
Position (kb)
1974
1975
1976 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1973.492 kb on - strand, within ABZR87_RS09310 at 1973.524 kb on + strand, within ABZR87_RS09310 at 1973.524 kb on + strand, within ABZR87_RS09310 at 1973.524 kb on + strand, within ABZR87_RS09310 at 1973.524 kb on + strand, within ABZR87_RS09310 at 1973.525 kb on - strand, within ABZR87_RS09310 at 1973.525 kb on - strand, within ABZR87_RS09310 at 1973.605 kb on + strand, within ABZR87_RS09310 at 1973.606 kb on - strand, within ABZR87_RS09310 at 1973.663 kb on - strand, within ABZR87_RS09310 at 1973.663 kb on - strand, within ABZR87_RS09310 at 1973.671 kb on + strand, within ABZR87_RS09310 at 1973.671 kb on + strand, within ABZR87_RS09310 at 1973.671 kb on + strand, within ABZR87_RS09310 at 1973.671 kb on + strand, within ABZR87_RS09310 at 1973.737 kb on + strand, within ABZR87_RS09310 at 1973.738 kb on - strand, within ABZR87_RS09310 at 1973.738 kb on - strand, within ABZR87_RS09310 at 1973.738 kb on - strand, within ABZR87_RS09310 at 1973.764 kb on + strand, within ABZR87_RS09310 at 1973.764 kb on + strand, within ABZR87_RS09310 at 1973.764 kb on + strand, within ABZR87_RS09310 at 1973.765 kb on - strand, within ABZR87_RS09310 at 1973.765 kb on - strand, within ABZR87_RS09310 at 1973.773 kb on + strand, within ABZR87_RS09310 at 1973.774 kb on - strand, within ABZR87_RS09310 at 1973.879 kb on - strand, within ABZR87_RS09310 at 1973.879 kb on - strand, within ABZR87_RS09310 at 1973.879 kb on - strand, within ABZR87_RS09310 at 1973.917 kb on + strand, within ABZR87_RS09310 at 1973.918 kb on - strand, within ABZR87_RS09310 at 1973.942 kb on - strand, within ABZR87_RS09310 at 1974.148 kb on + strand, within ABZR87_RS09310 at 1974.148 kb on + strand, within ABZR87_RS09310 at 1974.148 kb on + strand, within ABZR87_RS09310 at 1974.148 kb on + strand, within ABZR87_RS09310 at 1974.149 kb on - strand, within ABZR87_RS09310 at 1974.149 kb on - strand, within ABZR87_RS09310 at 1974.149 kb on - strand, within ABZR87_RS09310 at 1974.149 kb on - strand, within ABZR87_RS09310 at 1974.193 kb on + strand, within ABZR87_RS09310 at 1974.194 kb on - strand, within ABZR87_RS09310 at 1974.194 kb on - strand, within ABZR87_RS09310 at 1974.194 kb on - strand, within ABZR87_RS09310 at 1974.194 kb on - strand, within ABZR87_RS09310 at 1974.194 kb on - strand, within ABZR87_RS09310 at 1974.196 kb on + strand, within ABZR87_RS09310 at 1974.197 kb on - strand, within ABZR87_RS09310 at 1974.197 kb on - strand, within ABZR87_RS09310 at 1974.197 kb on - strand, within ABZR87_RS09310 at 1974.197 kb on - strand, within ABZR87_RS09310 at 1974.275 kb on - strand at 1974.275 kb on - strand at 1974.464 kb on + strand at 1974.464 kb on + strand at 1974.465 kb on - strand at 1974.465 kb on - strand at 1974.465 kb on - strand at 1974.534 kb on + strand, within ABZR87_RS09315 at 1974.588 kb on - strand, within ABZR87_RS09315 at 1974.921 kb on + strand, within ABZR87_RS09315 at 1974.922 kb on - strand, within ABZR87_RS09315 at 1974.947 kb on + strand, within ABZR87_RS09315 at 1974.947 kb on + strand, within ABZR87_RS09315 at 1974.947 kb on + strand, within ABZR87_RS09315 at 1974.948 kb on - strand, within ABZR87_RS09315 at 1974.948 kb on - strand, within ABZR87_RS09315 at 1974.948 kb on - strand, within ABZR87_RS09315 at 1974.948 kb on - strand, within ABZR87_RS09315 at 1975.007 kb on + strand, within ABZR87_RS09315 at 1975.007 kb on + strand, within ABZR87_RS09315 at 1975.007 kb on + strand, within ABZR87_RS09315 at 1975.008 kb on - strand, within ABZR87_RS09315 at 1975.008 kb on - strand, within ABZR87_RS09315 at 1975.104 kb on + strand, within ABZR87_RS09315 at 1975.182 kb on + strand at 1975.182 kb on + strand at 1975.183 kb on - strand at 1975.524 kb on - strand, within ABZR87_RS09320 at 1975.651 kb on + strand, within ABZR87_RS09320 at 1975.652 kb on - strand, within ABZR87_RS09320 at 1975.652 kb on - strand, within ABZR87_RS09320 at 1975.652 kb on - strand, within ABZR87_RS09320 at 1975.679 kb on - strand, within ABZR87_RS09320 at 1975.760 kb on - strand, within ABZR87_RS09320 at 1975.760 kb on - strand, within ABZR87_RS09320 at 1976.062 kb on + strand, within ABZR87_RS09320 at 1976.062 kb on + strand, within ABZR87_RS09320 at 1976.062 kb on + strand, within ABZR87_RS09320 at 1976.062 kb on + strand, within ABZR87_RS09320 at 1976.062 kb on + strand, within ABZR87_RS09320 at 1976.063 kb on - strand, within ABZR87_RS09320 at 1976.063 kb on - strand, within ABZR87_RS09320 at 1976.063 kb on - strand, within ABZR87_RS09320 at 1976.063 kb on - strand, within ABZR87_RS09320 at 1976.063 kb on - strand, within ABZR87_RS09320
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,973,492 - ABZR87_RS09310 0.56 +0.3 1,973,524 + ABZR87_RS09310 0.57 -1.3 1,973,524 + ABZR87_RS09310 0.57 -2.0 1,973,524 + ABZR87_RS09310 0.57 -0.2 1,973,524 + ABZR87_RS09310 0.57 -1.4 1,973,525 - ABZR87_RS09310 0.57 +0.1 1,973,525 - ABZR87_RS09310 0.57 -0.3 1,973,605 + ABZR87_RS09310 0.61 -0.1 1,973,606 - ABZR87_RS09310 0.61 -2.8 1,973,663 - ABZR87_RS09310 0.64 -0.6 1,973,663 - ABZR87_RS09310 0.64 +0.9 1,973,671 + ABZR87_RS09310 0.64 +1.4 1,973,671 + ABZR87_RS09310 0.64 +0.2 1,973,671 + ABZR87_RS09310 0.64 -0.4 1,973,671 + ABZR87_RS09310 0.64 -0.1 1,973,737 + ABZR87_RS09310 0.67 +0.1 1,973,738 - ABZR87_RS09310 0.67 -1.3 1,973,738 - ABZR87_RS09310 0.67 +0.4 1,973,738 - ABZR87_RS09310 0.67 +0.7 1,973,764 + ABZR87_RS09310 0.69 -0.5 1,973,764 + ABZR87_RS09310 0.69 -1.0 1,973,764 + ABZR87_RS09310 0.69 -1.0 1,973,765 - ABZR87_RS09310 0.69 -0.3 1,973,765 - ABZR87_RS09310 0.69 -0.3 1,973,773 + ABZR87_RS09310 0.69 +0.1 1,973,774 - ABZR87_RS09310 0.69 -2.0 1,973,879 - ABZR87_RS09310 0.74 -0.0 1,973,879 - ABZR87_RS09310 0.74 +0.3 1,973,879 - ABZR87_RS09310 0.74 -0.1 1,973,917 + ABZR87_RS09310 0.76 -0.0 1,973,918 - ABZR87_RS09310 0.76 -1.5 1,973,942 - ABZR87_RS09310 0.77 +0.2 1,974,148 + ABZR87_RS09310 0.87 +0.4 1,974,148 + ABZR87_RS09310 0.87 -0.9 1,974,148 + ABZR87_RS09310 0.87 +0.2 1,974,148 + ABZR87_RS09310 0.87 +0.7 1,974,149 - ABZR87_RS09310 0.87 +0.2 1,974,149 - ABZR87_RS09310 0.87 -0.7 1,974,149 - ABZR87_RS09310 0.87 +0.4 1,974,149 - ABZR87_RS09310 0.87 -0.7 1,974,193 + ABZR87_RS09310 0.89 -1.4 1,974,194 - ABZR87_RS09310 0.89 +0.1 1,974,194 - ABZR87_RS09310 0.89 +0.6 1,974,194 - ABZR87_RS09310 0.89 +1.2 1,974,194 - ABZR87_RS09310 0.89 +1.2 1,974,194 - ABZR87_RS09310 0.89 +1.7 1,974,196 + ABZR87_RS09310 0.89 -0.4 1,974,197 - ABZR87_RS09310 0.89 +0.5 1,974,197 - ABZR87_RS09310 0.89 +0.2 1,974,197 - ABZR87_RS09310 0.89 -0.7 1,974,197 - ABZR87_RS09310 0.89 -0.4 1,974,275 - -1.3 1,974,275 - -0.7 1,974,464 + -0.3 1,974,464 + -0.2 1,974,465 - -0.8 1,974,465 - -0.0 1,974,465 - -0.5 1,974,534 + ABZR87_RS09315 0.14 -2.3 1,974,588 - ABZR87_RS09315 0.21 -0.1 1,974,921 + ABZR87_RS09315 0.65 -1.0 1,974,922 - ABZR87_RS09315 0.65 +0.6 1,974,947 + ABZR87_RS09315 0.68 -0.4 1,974,947 + ABZR87_RS09315 0.68 -0.1 1,974,947 + ABZR87_RS09315 0.68 -0.6 1,974,948 - ABZR87_RS09315 0.68 -0.4 1,974,948 - ABZR87_RS09315 0.68 -1.8 1,974,948 - ABZR87_RS09315 0.68 +0.0 1,974,948 - ABZR87_RS09315 0.68 +1.9 1,975,007 + ABZR87_RS09315 0.76 -0.5 1,975,007 + ABZR87_RS09315 0.76 -0.9 1,975,007 + ABZR87_RS09315 0.76 +0.4 1,975,008 - ABZR87_RS09315 0.76 +0.8 1,975,008 - ABZR87_RS09315 0.76 +1.2 1,975,104 + ABZR87_RS09315 0.89 -1.5 1,975,182 + -0.9 1,975,182 + +0.0 1,975,183 - +0.6 1,975,524 - ABZR87_RS09320 0.23 -0.2 1,975,651 + ABZR87_RS09320 0.33 +0.2 1,975,652 - ABZR87_RS09320 0.33 -1.0 1,975,652 - ABZR87_RS09320 0.33 -2.0 1,975,652 - ABZR87_RS09320 0.33 -0.5 1,975,679 - ABZR87_RS09320 0.35 -0.4 1,975,760 - ABZR87_RS09320 0.41 -0.0 1,975,760 - ABZR87_RS09320 0.41 +0.1 1,976,062 + ABZR87_RS09320 0.62 -0.9 1,976,062 + ABZR87_RS09320 0.62 +0.4 1,976,062 + ABZR87_RS09320 0.62 -0.1 1,976,062 + ABZR87_RS09320 0.62 -0.1 1,976,062 + ABZR87_RS09320 0.62 -0.7 1,976,063 - ABZR87_RS09320 0.63 -1.1 1,976,063 - ABZR87_RS09320 0.63 -0.4 1,976,063 - ABZR87_RS09320 0.63 -1.2 1,976,063 - ABZR87_RS09320 0.63 -0.6 1,976,063 - ABZR87_RS09320 0.63 -0.6
Or see this region's nucleotide sequence