Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS09300 and ABZR87_RS09305 overlap by 4 nucleotides ABZR87_RS09305 and ABZR87_RS09310 are separated by 233 nucleotides
ABZR87_RS09300: ABZR87_RS09300 - hypothetical protein, at 1,969,920 to 1,971,035
_RS09300
ABZR87_RS09305: ABZR87_RS09305 - purine nucleoside permease, at 1,971,032 to 1,972,081
_RS09305
ABZR87_RS09310: ABZR87_RS09310 - TonB-dependent siderophore receptor, at 1,972,315 to 1,974,423
_RS09310
Position (kb)
1971
1972
1973 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1970.292 kb on + strand, within ABZR87_RS09300 at 1970.293 kb on - strand, within ABZR87_RS09300 at 1970.323 kb on - strand, within ABZR87_RS09300 at 1970.323 kb on - strand, within ABZR87_RS09300 at 1970.511 kb on + strand, within ABZR87_RS09300 at 1970.511 kb on + strand, within ABZR87_RS09300 at 1970.511 kb on + strand, within ABZR87_RS09300 at 1970.512 kb on - strand, within ABZR87_RS09300 at 1970.623 kb on - strand, within ABZR87_RS09300 at 1970.623 kb on - strand, within ABZR87_RS09300 at 1970.623 kb on - strand, within ABZR87_RS09300 at 1970.688 kb on + strand, within ABZR87_RS09300 at 1970.688 kb on + strand, within ABZR87_RS09300 at 1970.688 kb on + strand, within ABZR87_RS09300 at 1970.689 kb on - strand, within ABZR87_RS09300 at 1970.689 kb on - strand, within ABZR87_RS09300 at 1970.760 kb on + strand, within ABZR87_RS09300 at 1970.760 kb on + strand, within ABZR87_RS09300 at 1970.760 kb on + strand, within ABZR87_RS09300 at 1970.760 kb on + strand, within ABZR87_RS09300 at 1970.760 kb on + strand, within ABZR87_RS09300 at 1970.761 kb on - strand, within ABZR87_RS09300 at 1970.761 kb on - strand, within ABZR87_RS09300 at 1970.761 kb on - strand, within ABZR87_RS09300 at 1970.884 kb on - strand, within ABZR87_RS09300 at 1970.901 kb on + strand, within ABZR87_RS09300 at 1970.902 kb on - strand, within ABZR87_RS09300 at 1970.902 kb on - strand, within ABZR87_RS09300 at 1970.958 kb on + strand at 1970.959 kb on - strand at 1971.073 kb on + strand at 1971.074 kb on - strand at 1971.074 kb on - strand at 1971.187 kb on + strand, within ABZR87_RS09305 at 1971.188 kb on - strand, within ABZR87_RS09305 at 1971.198 kb on - strand, within ABZR87_RS09305 at 1971.218 kb on + strand, within ABZR87_RS09305 at 1971.218 kb on + strand, within ABZR87_RS09305 at 1971.218 kb on + strand, within ABZR87_RS09305 at 1971.218 kb on + strand, within ABZR87_RS09305 at 1971.218 kb on + strand, within ABZR87_RS09305 at 1971.219 kb on - strand, within ABZR87_RS09305 at 1971.219 kb on - strand, within ABZR87_RS09305 at 1971.219 kb on - strand, within ABZR87_RS09305 at 1971.219 kb on - strand, within ABZR87_RS09305 at 1971.219 kb on - strand, within ABZR87_RS09305 at 1971.219 kb on - strand, within ABZR87_RS09305 at 1971.220 kb on + strand, within ABZR87_RS09305 at 1971.220 kb on + strand, within ABZR87_RS09305 at 1971.221 kb on - strand, within ABZR87_RS09305 at 1971.263 kb on + strand, within ABZR87_RS09305 at 1971.263 kb on + strand, within ABZR87_RS09305 at 1971.264 kb on - strand, within ABZR87_RS09305 at 1971.264 kb on - strand, within ABZR87_RS09305 at 1971.264 kb on - strand, within ABZR87_RS09305 at 1971.264 kb on - strand, within ABZR87_RS09305 at 1971.299 kb on + strand, within ABZR87_RS09305 at 1971.299 kb on + strand, within ABZR87_RS09305 at 1971.300 kb on - strand, within ABZR87_RS09305 at 1971.300 kb on - strand, within ABZR87_RS09305 at 1971.365 kb on + strand, within ABZR87_RS09305 at 1971.365 kb on + strand, within ABZR87_RS09305 at 1971.365 kb on + strand, within ABZR87_RS09305 at 1971.365 kb on + strand, within ABZR87_RS09305 at 1971.365 kb on + strand, within ABZR87_RS09305 at 1971.365 kb on + strand, within ABZR87_RS09305 at 1971.365 kb on + strand, within ABZR87_RS09305 at 1971.365 kb on + strand, within ABZR87_RS09305 at 1971.501 kb on - strand, within ABZR87_RS09305 at 1971.506 kb on + strand, within ABZR87_RS09305 at 1971.507 kb on - strand, within ABZR87_RS09305 at 1971.555 kb on - strand, within ABZR87_RS09305 at 1971.602 kb on + strand, within ABZR87_RS09305 at 1971.602 kb on + strand, within ABZR87_RS09305 at 1971.603 kb on - strand, within ABZR87_RS09305 at 1971.603 kb on - strand, within ABZR87_RS09305 at 1971.665 kb on + strand, within ABZR87_RS09305 at 1971.665 kb on + strand, within ABZR87_RS09305 at 1971.665 kb on + strand, within ABZR87_RS09305 at 1971.665 kb on + strand, within ABZR87_RS09305 at 1971.666 kb on - strand, within ABZR87_RS09305 at 1971.671 kb on + strand, within ABZR87_RS09305 at 1971.803 kb on + strand, within ABZR87_RS09305 at 1971.803 kb on + strand, within ABZR87_RS09305 at 1971.804 kb on - strand, within ABZR87_RS09305 at 1971.804 kb on - strand, within ABZR87_RS09305 at 1971.927 kb on - strand, within ABZR87_RS09305 at 1971.927 kb on - strand, within ABZR87_RS09305 at 1971.962 kb on + strand, within ABZR87_RS09305 at 1971.963 kb on - strand, within ABZR87_RS09305 at 1972.080 kb on - strand at 1972.080 kb on - strand at 1972.486 kb on - strand at 1972.486 kb on - strand at 1972.608 kb on + strand, within ABZR87_RS09310 at 1972.792 kb on + strand, within ABZR87_RS09310 at 1972.793 kb on - strand, within ABZR87_RS09310 at 1972.793 kb on - strand, within ABZR87_RS09310 at 1972.793 kb on - strand, within ABZR87_RS09310 at 1972.795 kb on + strand, within ABZR87_RS09310 at 1972.795 kb on + strand, within ABZR87_RS09310 at 1972.796 kb on - strand, within ABZR87_RS09310 at 1972.796 kb on - strand, within ABZR87_RS09310 at 1972.796 kb on - strand, within ABZR87_RS09310 at 1972.801 kb on + strand, within ABZR87_RS09310 at 1972.802 kb on - strand, within ABZR87_RS09310 at 1972.946 kb on - strand, within ABZR87_RS09310 at 1972.990 kb on - strand, within ABZR87_RS09310 at 1973.080 kb on + strand, within ABZR87_RS09310
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,970,292 + ABZR87_RS09300 0.33 -3.6 1,970,293 - ABZR87_RS09300 0.33 +0.0 1,970,323 - ABZR87_RS09300 0.36 +0.2 1,970,323 - ABZR87_RS09300 0.36 -0.1 1,970,511 + ABZR87_RS09300 0.53 -0.2 1,970,511 + ABZR87_RS09300 0.53 -1.1 1,970,511 + ABZR87_RS09300 0.53 -0.5 1,970,512 - ABZR87_RS09300 0.53 -0.3 1,970,623 - ABZR87_RS09300 0.63 +0.1 1,970,623 - ABZR87_RS09300 0.63 +1.6 1,970,623 - ABZR87_RS09300 0.63 +0.7 1,970,688 + ABZR87_RS09300 0.69 +0.3 1,970,688 + ABZR87_RS09300 0.69 +0.3 1,970,688 + ABZR87_RS09300 0.69 +0.4 1,970,689 - ABZR87_RS09300 0.69 -0.1 1,970,689 - ABZR87_RS09300 0.69 -0.9 1,970,760 + ABZR87_RS09300 0.75 +1.2 1,970,760 + ABZR87_RS09300 0.75 -0.3 1,970,760 + ABZR87_RS09300 0.75 +1.7 1,970,760 + ABZR87_RS09300 0.75 -0.7 1,970,760 + ABZR87_RS09300 0.75 -1.1 1,970,761 - ABZR87_RS09300 0.75 +0.2 1,970,761 - ABZR87_RS09300 0.75 -0.4 1,970,761 - ABZR87_RS09300 0.75 +0.1 1,970,884 - ABZR87_RS09300 0.86 -0.8 1,970,901 + ABZR87_RS09300 0.88 +0.3 1,970,902 - ABZR87_RS09300 0.88 +0.4 1,970,902 - ABZR87_RS09300 0.88 -0.6 1,970,958 + +2.5 1,970,959 - +0.3 1,971,073 + -2.1 1,971,074 - +0.4 1,971,074 - -0.4 1,971,187 + ABZR87_RS09305 0.15 +0.6 1,971,188 - ABZR87_RS09305 0.15 +1.0 1,971,198 - ABZR87_RS09305 0.16 +0.7 1,971,218 + ABZR87_RS09305 0.18 +0.3 1,971,218 + ABZR87_RS09305 0.18 -0.0 1,971,218 + ABZR87_RS09305 0.18 +0.4 1,971,218 + ABZR87_RS09305 0.18 -0.5 1,971,218 + ABZR87_RS09305 0.18 +0.1 1,971,219 - ABZR87_RS09305 0.18 +0.1 1,971,219 - ABZR87_RS09305 0.18 +0.5 1,971,219 - ABZR87_RS09305 0.18 -0.1 1,971,219 - ABZR87_RS09305 0.18 -0.2 1,971,219 - ABZR87_RS09305 0.18 -1.6 1,971,219 - ABZR87_RS09305 0.18 -0.2 1,971,220 + ABZR87_RS09305 0.18 -1.5 1,971,220 + ABZR87_RS09305 0.18 -0.4 1,971,221 - ABZR87_RS09305 0.18 -0.3 1,971,263 + ABZR87_RS09305 0.22 -0.2 1,971,263 + ABZR87_RS09305 0.22 -0.4 1,971,264 - ABZR87_RS09305 0.22 -0.2 1,971,264 - ABZR87_RS09305 0.22 -0.5 1,971,264 - ABZR87_RS09305 0.22 -0.4 1,971,264 - ABZR87_RS09305 0.22 +0.9 1,971,299 + ABZR87_RS09305 0.25 +0.4 1,971,299 + ABZR87_RS09305 0.25 -0.5 1,971,300 - ABZR87_RS09305 0.26 -1.4 1,971,300 - ABZR87_RS09305 0.26 +0.2 1,971,365 + ABZR87_RS09305 0.32 +0.6 1,971,365 + ABZR87_RS09305 0.32 -0.7 1,971,365 + ABZR87_RS09305 0.32 -0.5 1,971,365 + ABZR87_RS09305 0.32 -0.3 1,971,365 + ABZR87_RS09305 0.32 -0.7 1,971,365 + ABZR87_RS09305 0.32 -0.0 1,971,365 + ABZR87_RS09305 0.32 +0.7 1,971,365 + ABZR87_RS09305 0.32 +0.1 1,971,501 - ABZR87_RS09305 0.45 -0.7 1,971,506 + ABZR87_RS09305 0.45 +1.1 1,971,507 - ABZR87_RS09305 0.45 -1.2 1,971,555 - ABZR87_RS09305 0.50 +1.2 1,971,602 + ABZR87_RS09305 0.54 -0.6 1,971,602 + ABZR87_RS09305 0.54 -0.1 1,971,603 - ABZR87_RS09305 0.54 -0.9 1,971,603 - ABZR87_RS09305 0.54 -0.4 1,971,665 + ABZR87_RS09305 0.60 -0.9 1,971,665 + ABZR87_RS09305 0.60 +0.1 1,971,665 + ABZR87_RS09305 0.60 +1.2 1,971,665 + ABZR87_RS09305 0.60 -0.2 1,971,666 - ABZR87_RS09305 0.60 -0.1 1,971,671 + ABZR87_RS09305 0.61 -1.8 1,971,803 + ABZR87_RS09305 0.73 -1.6 1,971,803 + ABZR87_RS09305 0.73 -3.1 1,971,804 - ABZR87_RS09305 0.74 -0.2 1,971,804 - ABZR87_RS09305 0.74 +0.2 1,971,927 - ABZR87_RS09305 0.85 +0.3 1,971,927 - ABZR87_RS09305 0.85 -0.0 1,971,962 + ABZR87_RS09305 0.89 -0.7 1,971,963 - ABZR87_RS09305 0.89 -0.7 1,972,080 - +0.1 1,972,080 - -0.4 1,972,486 - +1.6 1,972,486 - +0.4 1,972,608 + ABZR87_RS09310 0.14 +0.2 1,972,792 + ABZR87_RS09310 0.23 -0.1 1,972,793 - ABZR87_RS09310 0.23 -0.5 1,972,793 - ABZR87_RS09310 0.23 -0.0 1,972,793 - ABZR87_RS09310 0.23 -1.5 1,972,795 + ABZR87_RS09310 0.23 -0.0 1,972,795 + ABZR87_RS09310 0.23 -0.2 1,972,796 - ABZR87_RS09310 0.23 -0.8 1,972,796 - ABZR87_RS09310 0.23 -0.6 1,972,796 - ABZR87_RS09310 0.23 -2.3 1,972,801 + ABZR87_RS09310 0.23 -1.1 1,972,802 - ABZR87_RS09310 0.23 -0.0 1,972,946 - ABZR87_RS09310 0.30 -0.7 1,972,990 - ABZR87_RS09310 0.32 -0.4 1,973,080 + ABZR87_RS09310 0.36 +0.0
Or see this region's nucleotide sequence