Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS08445 and ABZR87_RS08450 are separated by 101 nucleotides ABZR87_RS08450 and ABZR87_RS08455 are separated by 257 nucleotides
ABZR87_RS08445: ABZR87_RS08445 - co-chaperone GroES, at 1,793,834 to 1,794,124
_RS08445
ABZR87_RS08450: ABZR87_RS08450 - chaperonin GroEL, at 1,794,226 to 1,795,869
_RS08450
ABZR87_RS08455: ABZR87_RS08455 - YjgN family protein, at 1,796,127 to 1,797,209
_RS08455
Position (kb)
1794
1795
1796 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1793.361 kb on + strand at 1793.362 kb on - strand at 1793.395 kb on + strand at 1793.406 kb on - strand at 1793.449 kb on - strand at 1793.520 kb on + strand at 1793.520 kb on + strand at 1793.527 kb on + strand at 1793.528 kb on - strand at 1793.567 kb on - strand at 1793.619 kb on - strand at 1793.700 kb on + strand at 1793.700 kb on + strand at 1793.700 kb on + strand at 1793.712 kb on - strand at 1793.713 kb on + strand at 1793.713 kb on + strand at 1793.717 kb on + strand at 1793.717 kb on + strand at 1793.740 kb on + strand at 1793.740 kb on + strand at 1793.740 kb on + strand at 1793.740 kb on + strand at 1794.122 kb on + strand at 1794.122 kb on + strand at 1794.147 kb on + strand at 1795.867 kb on + strand at 1795.867 kb on + strand at 1795.867 kb on + strand at 1795.867 kb on + strand at 1795.867 kb on + strand at 1795.867 kb on + strand at 1795.868 kb on - strand at 1795.868 kb on - strand at 1795.971 kb on + strand at 1795.971 kb on + strand at 1795.972 kb on - strand at 1796.122 kb on + strand at 1796.122 kb on + strand at 1796.123 kb on - strand at 1796.123 kb on - strand at 1796.123 kb on - strand at 1796.123 kb on - strand at 1796.123 kb on - strand at 1796.123 kb on - strand at 1796.286 kb on + strand, within ABZR87_RS08455 at 1796.286 kb on + strand, within ABZR87_RS08455 at 1796.286 kb on + strand, within ABZR87_RS08455 at 1796.287 kb on - strand, within ABZR87_RS08455 at 1796.287 kb on - strand, within ABZR87_RS08455 at 1796.319 kb on + strand, within ABZR87_RS08455 at 1796.320 kb on - strand, within ABZR87_RS08455 at 1796.320 kb on - strand, within ABZR87_RS08455 at 1796.320 kb on - strand, within ABZR87_RS08455 at 1796.362 kb on - strand, within ABZR87_RS08455 at 1796.362 kb on - strand, within ABZR87_RS08455 at 1796.506 kb on + strand, within ABZR87_RS08455 at 1796.506 kb on + strand, within ABZR87_RS08455 at 1796.507 kb on - strand, within ABZR87_RS08455 at 1796.586 kb on + strand, within ABZR87_RS08455 at 1796.586 kb on + strand, within ABZR87_RS08455 at 1796.586 kb on + strand, within ABZR87_RS08455 at 1796.586 kb on + strand, within ABZR87_RS08455 at 1796.586 kb on + strand, within ABZR87_RS08455 at 1796.587 kb on - strand, within ABZR87_RS08455 at 1796.632 kb on - strand, within ABZR87_RS08455 at 1796.632 kb on - strand, within ABZR87_RS08455 at 1796.735 kb on + strand, within ABZR87_RS08455 at 1796.736 kb on + strand, within ABZR87_RS08455 at 1796.736 kb on + strand, within ABZR87_RS08455 at 1796.737 kb on - strand, within ABZR87_RS08455 at 1796.737 kb on - strand, within ABZR87_RS08455 at 1796.737 kb on - strand, within ABZR87_RS08455 at 1796.737 kb on - strand, within ABZR87_RS08455 at 1796.737 kb on - strand, within ABZR87_RS08455 at 1796.745 kb on + strand, within ABZR87_RS08455 at 1796.745 kb on + strand, within ABZR87_RS08455 at 1796.745 kb on + strand, within ABZR87_RS08455 at 1796.746 kb on - strand, within ABZR87_RS08455 at 1796.771 kb on - strand, within ABZR87_RS08455 at 1796.771 kb on - strand, within ABZR87_RS08455
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,793,361 + +1.2 1,793,362 - +0.1 1,793,395 + -0.5 1,793,406 - -0.7 1,793,449 - -0.1 1,793,520 + +0.6 1,793,520 + +0.3 1,793,527 + +0.6 1,793,528 - -0.2 1,793,567 - +0.3 1,793,619 - +0.1 1,793,700 + -0.3 1,793,700 + -1.3 1,793,700 + -0.4 1,793,712 - -0.4 1,793,713 + -1.2 1,793,713 + -1.8 1,793,717 + -0.4 1,793,717 + -0.2 1,793,740 + +0.4 1,793,740 + -0.8 1,793,740 + +0.9 1,793,740 + -0.6 1,794,122 + +0.4 1,794,122 + +2.3 1,794,147 + -0.6 1,795,867 + -0.6 1,795,867 + +0.8 1,795,867 + +0.9 1,795,867 + +0.0 1,795,867 + -0.8 1,795,867 + -2.8 1,795,868 - +0.7 1,795,868 - -0.4 1,795,971 + +0.1 1,795,971 + -0.5 1,795,972 - -0.7 1,796,122 + +0.5 1,796,122 + +0.8 1,796,123 - +0.7 1,796,123 - +0.5 1,796,123 - -0.2 1,796,123 - -1.5 1,796,123 - -0.3 1,796,123 - -0.6 1,796,286 + ABZR87_RS08455 0.15 -0.5 1,796,286 + ABZR87_RS08455 0.15 -0.5 1,796,286 + ABZR87_RS08455 0.15 -0.7 1,796,287 - ABZR87_RS08455 0.15 -0.3 1,796,287 - ABZR87_RS08455 0.15 +0.3 1,796,319 + ABZR87_RS08455 0.18 -0.2 1,796,320 - ABZR87_RS08455 0.18 -0.2 1,796,320 - ABZR87_RS08455 0.18 -0.7 1,796,320 - ABZR87_RS08455 0.18 -0.4 1,796,362 - ABZR87_RS08455 0.22 +0.2 1,796,362 - ABZR87_RS08455 0.22 -0.3 1,796,506 + ABZR87_RS08455 0.35 -0.1 1,796,506 + ABZR87_RS08455 0.35 -0.0 1,796,507 - ABZR87_RS08455 0.35 -0.4 1,796,586 + ABZR87_RS08455 0.42 -0.8 1,796,586 + ABZR87_RS08455 0.42 +0.3 1,796,586 + ABZR87_RS08455 0.42 -0.3 1,796,586 + ABZR87_RS08455 0.42 +0.6 1,796,586 + ABZR87_RS08455 0.42 +0.3 1,796,587 - ABZR87_RS08455 0.42 -1.0 1,796,632 - ABZR87_RS08455 0.47 +0.4 1,796,632 - ABZR87_RS08455 0.47 -0.1 1,796,735 + ABZR87_RS08455 0.56 +0.3 1,796,736 + ABZR87_RS08455 0.56 -1.5 1,796,736 + ABZR87_RS08455 0.56 -0.4 1,796,737 - ABZR87_RS08455 0.56 +0.0 1,796,737 - ABZR87_RS08455 0.56 -1.0 1,796,737 - ABZR87_RS08455 0.56 +0.4 1,796,737 - ABZR87_RS08455 0.56 -1.1 1,796,737 - ABZR87_RS08455 0.56 -1.7 1,796,745 + ABZR87_RS08455 0.57 +0.5 1,796,745 + ABZR87_RS08455 0.57 -0.7 1,796,745 + ABZR87_RS08455 0.57 -1.3 1,796,746 - ABZR87_RS08455 0.57 +0.2 1,796,771 - ABZR87_RS08455 0.59 +0.5 1,796,771 - ABZR87_RS08455 0.59 -0.1
Or see this region's nucleotide sequence