Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS08165 and ABZR87_RS08170 are separated by 27 nucleotides ABZR87_RS08170 and ABZR87_RS08175 are separated by 45 nucleotides ABZR87_RS08175 and ABZR87_RS08180 are separated by 175 nucleotides
ABZR87_RS08165: ABZR87_RS08165 - amidase, at 1,737,191 to 1,738,549
_RS08165
ABZR87_RS08170: ABZR87_RS08170 - DUF2848 domain-containing protein, at 1,738,577 to 1,739,254
_RS08170
ABZR87_RS08175: ABZR87_RS08175 - GntR family transcriptional regulator, at 1,739,300 to 1,740,016
_RS08175
ABZR87_RS08180: ABZR87_RS08180 - flavin-dependent oxidoreductase, at 1,740,192 to 1,741,457
_RS08180
Position (kb)
1738
1739
1740 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1737.809 kb on + strand, within ABZR87_RS08165 at 1737.810 kb on - strand, within ABZR87_RS08165 at 1738.416 kb on + strand at 1738.417 kb on - strand at 1738.417 kb on - strand at 1738.608 kb on + strand at 1738.609 kb on - strand at 1738.712 kb on - strand, within ABZR87_RS08170 at 1738.800 kb on + strand, within ABZR87_RS08170 at 1738.935 kb on + strand, within ABZR87_RS08170 at 1738.935 kb on + strand, within ABZR87_RS08170 at 1738.935 kb on + strand, within ABZR87_RS08170 at 1739.001 kb on + strand, within ABZR87_RS08170 at 1739.002 kb on - strand, within ABZR87_RS08170 at 1739.088 kb on + strand, within ABZR87_RS08170 at 1739.088 kb on + strand, within ABZR87_RS08170 at 1739.088 kb on + strand, within ABZR87_RS08170 at 1739.088 kb on + strand, within ABZR87_RS08170 at 1739.088 kb on + strand, within ABZR87_RS08170 at 1739.089 kb on - strand, within ABZR87_RS08170 at 1739.089 kb on - strand, within ABZR87_RS08170 at 1739.089 kb on - strand, within ABZR87_RS08170 at 1739.415 kb on + strand, within ABZR87_RS08175 at 1739.415 kb on + strand, within ABZR87_RS08175 at 1739.415 kb on + strand, within ABZR87_RS08175 at 1739.904 kb on + strand, within ABZR87_RS08175 at 1739.904 kb on + strand, within ABZR87_RS08175 at 1739.904 kb on + strand, within ABZR87_RS08175 at 1740.112 kb on + strand at 1740.112 kb on + strand at 1740.113 kb on - strand at 1740.116 kb on + strand at 1740.116 kb on + strand at 1740.116 kb on + strand at 1740.117 kb on - strand at 1740.117 kb on - strand at 1740.117 kb on - strand at 1740.117 kb on - strand at 1740.117 kb on - strand at 1740.128 kb on + strand at 1740.128 kb on + strand at 1740.129 kb on - strand at 1740.129 kb on - strand at 1740.129 kb on - strand at 1740.129 kb on - strand at 1740.129 kb on - strand at 1740.129 kb on - strand at 1740.129 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,737,809 + ABZR87_RS08165 0.45 -0.3 1,737,810 - ABZR87_RS08165 0.46 +0.9 1,738,416 + +0.1 1,738,417 - +0.9 1,738,417 - +0.1 1,738,608 + -0.0 1,738,609 - -0.6 1,738,712 - ABZR87_RS08170 0.20 +2.9 1,738,800 + ABZR87_RS08170 0.33 +0.5 1,738,935 + ABZR87_RS08170 0.53 +0.3 1,738,935 + ABZR87_RS08170 0.53 +0.5 1,738,935 + ABZR87_RS08170 0.53 +1.3 1,739,001 + ABZR87_RS08170 0.63 -0.7 1,739,002 - ABZR87_RS08170 0.63 +2.9 1,739,088 + ABZR87_RS08170 0.75 +0.4 1,739,088 + ABZR87_RS08170 0.75 -0.1 1,739,088 + ABZR87_RS08170 0.75 -0.5 1,739,088 + ABZR87_RS08170 0.75 -0.0 1,739,088 + ABZR87_RS08170 0.75 -0.4 1,739,089 - ABZR87_RS08170 0.76 -1.5 1,739,089 - ABZR87_RS08170 0.76 +0.4 1,739,089 - ABZR87_RS08170 0.76 -1.5 1,739,415 + ABZR87_RS08175 0.16 +0.2 1,739,415 + ABZR87_RS08175 0.16 -0.1 1,739,415 + ABZR87_RS08175 0.16 +0.8 1,739,904 + ABZR87_RS08175 0.84 -1.1 1,739,904 + ABZR87_RS08175 0.84 +1.9 1,739,904 + ABZR87_RS08175 0.84 +0.5 1,740,112 + -2.7 1,740,112 + -0.2 1,740,113 - -1.2 1,740,116 + +0.3 1,740,116 + -0.7 1,740,116 + -0.1 1,740,117 - -0.2 1,740,117 - +1.3 1,740,117 - +1.9 1,740,117 - +0.6 1,740,117 - -0.4 1,740,128 + +2.7 1,740,128 + +0.0 1,740,129 - +0.2 1,740,129 - +0.9 1,740,129 - -0.7 1,740,129 - +0.2 1,740,129 - +0.6 1,740,129 - +0.1 1,740,129 - +0.6
Or see this region's nucleotide sequence