Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS08160 and ABZR87_RS08165 are separated by 174 nucleotides ABZR87_RS08165 and ABZR87_RS08170 are separated by 27 nucleotides ABZR87_RS08170 and ABZR87_RS08175 are separated by 45 nucleotides
ABZR87_RS08160: ABZR87_RS08160 - MFS transporter, at 1,735,694 to 1,737,016
_RS08160
ABZR87_RS08165: ABZR87_RS08165 - amidase, at 1,737,191 to 1,738,549
_RS08165
ABZR87_RS08170: ABZR87_RS08170 - DUF2848 domain-containing protein, at 1,738,577 to 1,739,254
_RS08170
ABZR87_RS08175: ABZR87_RS08175 - GntR family transcriptional regulator, at 1,739,300 to 1,740,016
_RS08175
Position (kb) 1737
1738
1739 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1736.218 kb on - strand, within ABZR87_RS08160 at 1736.572 kb on - strand, within ABZR87_RS08160 at 1736.641 kb on - strand, within ABZR87_RS08160 at 1736.641 kb on - strand, within ABZR87_RS08160 at 1736.664 kb on + strand, within ABZR87_RS08160 at 1736.664 kb on + strand, within ABZR87_RS08160 at 1736.664 kb on + strand, within ABZR87_RS08160 at 1736.664 kb on + strand, within ABZR87_RS08160 at 1736.664 kb on + strand, within ABZR87_RS08160 at 1736.665 kb on - strand, within ABZR87_RS08160 at 1736.665 kb on - strand, within ABZR87_RS08160 at 1736.772 kb on + strand, within ABZR87_RS08160 at 1736.772 kb on + strand, within ABZR87_RS08160 at 1736.805 kb on + strand, within ABZR87_RS08160 at 1736.934 kb on + strand at 1736.935 kb on - strand at 1737.029 kb on + strand at 1737.029 kb on + strand at 1737.030 kb on - strand at 1737.030 kb on - strand at 1737.106 kb on - strand at 1737.514 kb on - strand, within ABZR87_RS08165 at 1737.514 kb on - strand, within ABZR87_RS08165 at 1737.514 kb on - strand, within ABZR87_RS08165 at 1737.570 kb on + strand, within ABZR87_RS08165 at 1737.571 kb on - strand, within ABZR87_RS08165 at 1737.809 kb on + strand, within ABZR87_RS08165 at 1737.810 kb on - strand, within ABZR87_RS08165 at 1738.416 kb on + strand at 1738.417 kb on - strand at 1738.417 kb on - strand at 1738.608 kb on + strand at 1738.609 kb on - strand at 1738.712 kb on - strand, within ABZR87_RS08170 at 1738.800 kb on + strand, within ABZR87_RS08170 at 1738.935 kb on + strand, within ABZR87_RS08170 at 1738.935 kb on + strand, within ABZR87_RS08170 at 1738.935 kb on + strand, within ABZR87_RS08170 at 1739.001 kb on + strand, within ABZR87_RS08170 at 1739.002 kb on - strand, within ABZR87_RS08170 at 1739.088 kb on + strand, within ABZR87_RS08170 at 1739.088 kb on + strand, within ABZR87_RS08170 at 1739.088 kb on + strand, within ABZR87_RS08170 at 1739.088 kb on + strand, within ABZR87_RS08170 at 1739.088 kb on + strand, within ABZR87_RS08170 at 1739.089 kb on - strand, within ABZR87_RS08170 at 1739.089 kb on - strand, within ABZR87_RS08170 at 1739.089 kb on - strand, within ABZR87_RS08170 at 1739.415 kb on + strand, within ABZR87_RS08175 at 1739.415 kb on + strand, within ABZR87_RS08175 at 1739.415 kb on + strand, within ABZR87_RS08175
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,736,218 - ABZR87_RS08160 0.40 +0.3 1,736,572 - ABZR87_RS08160 0.66 +0.2 1,736,641 - ABZR87_RS08160 0.72 -2.9 1,736,641 - ABZR87_RS08160 0.72 -0.1 1,736,664 + ABZR87_RS08160 0.73 -0.1 1,736,664 + ABZR87_RS08160 0.73 +0.2 1,736,664 + ABZR87_RS08160 0.73 -0.0 1,736,664 + ABZR87_RS08160 0.73 +0.6 1,736,664 + ABZR87_RS08160 0.73 -1.1 1,736,665 - ABZR87_RS08160 0.73 +0.7 1,736,665 - ABZR87_RS08160 0.73 -1.8 1,736,772 + ABZR87_RS08160 0.81 -0.8 1,736,772 + ABZR87_RS08160 0.81 -0.2 1,736,805 + ABZR87_RS08160 0.84 +0.3 1,736,934 + +0.6 1,736,935 - -0.1 1,737,029 + -0.0 1,737,029 + +1.1 1,737,030 - +0.1 1,737,030 - +0.4 1,737,106 - +0.1 1,737,514 - ABZR87_RS08165 0.24 -1.0 1,737,514 - ABZR87_RS08165 0.24 +0.4 1,737,514 - ABZR87_RS08165 0.24 -1.4 1,737,570 + ABZR87_RS08165 0.28 -0.4 1,737,571 - ABZR87_RS08165 0.28 +0.0 1,737,809 + ABZR87_RS08165 0.45 -0.3 1,737,810 - ABZR87_RS08165 0.46 +0.9 1,738,416 + +0.1 1,738,417 - +0.9 1,738,417 - +0.1 1,738,608 + -0.0 1,738,609 - -0.6 1,738,712 - ABZR87_RS08170 0.20 +2.9 1,738,800 + ABZR87_RS08170 0.33 +0.5 1,738,935 + ABZR87_RS08170 0.53 +0.3 1,738,935 + ABZR87_RS08170 0.53 +0.5 1,738,935 + ABZR87_RS08170 0.53 +1.3 1,739,001 + ABZR87_RS08170 0.63 -0.7 1,739,002 - ABZR87_RS08170 0.63 +2.9 1,739,088 + ABZR87_RS08170 0.75 +0.4 1,739,088 + ABZR87_RS08170 0.75 -0.1 1,739,088 + ABZR87_RS08170 0.75 -0.5 1,739,088 + ABZR87_RS08170 0.75 -0.0 1,739,088 + ABZR87_RS08170 0.75 -0.4 1,739,089 - ABZR87_RS08170 0.76 -1.5 1,739,089 - ABZR87_RS08170 0.76 +0.4 1,739,089 - ABZR87_RS08170 0.76 -1.5 1,739,415 + ABZR87_RS08175 0.16 +0.2 1,739,415 + ABZR87_RS08175 0.16 -0.1 1,739,415 + ABZR87_RS08175 0.16 +0.8
Or see this region's nucleotide sequence