Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS07915 and ABZR87_RS07920 are separated by 75 nucleotides ABZR87_RS07920 and ABZR87_RS07925 are separated by 48 nucleotides ABZR87_RS07925 and ABZR87_RS07930 are separated by 522 nucleotides ABZR87_RS07930 and ABZR87_RS07935 overlap by 4 nucleotides
ABZR87_RS07915: ABZR87_RS07915 - alpha/beta hydrolase, at 1,682,688 to 1,683,644
_RS07915
ABZR87_RS07920: ABZR87_RS07920 - tRNA-Met, at 1,683,720 to 1,683,796
_RS07920
ABZR87_RS07925: ABZR87_RS07925 - MFS transporter, at 1,683,845 to 1,685,548
_RS07925
ABZR87_RS07930: ABZR87_RS07930 - DUF485 domain-containing protein, at 1,686,071 to 1,686,379
_RS07930
ABZR87_RS07935: ABZR87_RS07935 - cation acetate symporter, at 1,686,376 to 1,688,106
_RS07935
Position (kb)
1683
1684
1685
1686 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1682.898 kb on + strand, within ABZR87_RS07915 at 1682.996 kb on + strand, within ABZR87_RS07915 at 1682.996 kb on + strand, within ABZR87_RS07915 at 1682.997 kb on - strand, within ABZR87_RS07915 at 1682.997 kb on - strand, within ABZR87_RS07915 at 1682.997 kb on - strand, within ABZR87_RS07915 at 1682.997 kb on - strand, within ABZR87_RS07915 at 1682.997 kb on - strand, within ABZR87_RS07915 at 1683.000 kb on + strand, within ABZR87_RS07915 at 1683.001 kb on - strand, within ABZR87_RS07915 at 1683.001 kb on - strand, within ABZR87_RS07915 at 1683.363 kb on + strand, within ABZR87_RS07915 at 1683.364 kb on - strand, within ABZR87_RS07915 at 1683.364 kb on - strand, within ABZR87_RS07915 at 1683.456 kb on + strand, within ABZR87_RS07915 at 1683.456 kb on + strand, within ABZR87_RS07915 at 1683.456 kb on + strand, within ABZR87_RS07915 at 1683.457 kb on - strand, within ABZR87_RS07915 at 1683.457 kb on - strand, within ABZR87_RS07915 at 1683.554 kb on + strand at 1683.554 kb on + strand at 1683.554 kb on + strand at 1683.554 kb on + strand at 1683.555 kb on - strand at 1683.555 kb on - strand at 1683.555 kb on - strand at 1683.555 kb on - strand at 1683.555 kb on - strand at 1683.642 kb on + strand at 1683.643 kb on - strand at 1683.717 kb on - strand at 1683.858 kb on + strand at 1683.859 kb on - strand at 1683.948 kb on + strand at 1683.948 kb on + strand at 1683.948 kb on + strand at 1683.948 kb on + strand at 1683.948 kb on + strand at 1683.948 kb on + strand at 1683.948 kb on + strand at 1683.948 kb on + strand at 1683.949 kb on - strand at 1683.949 kb on - strand at 1683.949 kb on - strand at 1683.950 kb on + strand at 1683.952 kb on + strand at 1683.952 kb on + strand at 1683.952 kb on + strand at 1683.952 kb on + strand at 1683.952 kb on + strand at 1683.952 kb on + strand at 1683.952 kb on + strand at 1684.026 kb on + strand, within ABZR87_RS07925 at 1684.027 kb on - strand, within ABZR87_RS07925 at 1684.047 kb on + strand, within ABZR87_RS07925 at 1684.113 kb on + strand, within ABZR87_RS07925 at 1684.113 kb on + strand, within ABZR87_RS07925 at 1684.113 kb on + strand, within ABZR87_RS07925 at 1684.113 kb on + strand, within ABZR87_RS07925 at 1684.114 kb on - strand, within ABZR87_RS07925 at 1684.114 kb on - strand, within ABZR87_RS07925 at 1684.114 kb on - strand, within ABZR87_RS07925 at 1684.114 kb on - strand, within ABZR87_RS07925 at 1684.186 kb on - strand, within ABZR87_RS07925 at 1684.359 kb on + strand, within ABZR87_RS07925 at 1684.359 kb on + strand, within ABZR87_RS07925 at 1684.359 kb on + strand, within ABZR87_RS07925 at 1684.360 kb on - strand, within ABZR87_RS07925 at 1684.384 kb on + strand, within ABZR87_RS07925 at 1684.384 kb on + strand, within ABZR87_RS07925 at 1684.521 kb on + strand, within ABZR87_RS07925 at 1684.551 kb on + strand, within ABZR87_RS07925 at 1684.552 kb on - strand, within ABZR87_RS07925 at 1684.552 kb on - strand, within ABZR87_RS07925 at 1684.552 kb on - strand, within ABZR87_RS07925 at 1684.552 kb on - strand, within ABZR87_RS07925 at 1684.726 kb on - strand, within ABZR87_RS07925 at 1684.741 kb on - strand, within ABZR87_RS07925 at 1684.741 kb on - strand, within ABZR87_RS07925 at 1684.902 kb on + strand, within ABZR87_RS07925 at 1684.902 kb on + strand, within ABZR87_RS07925 at 1684.902 kb on + strand, within ABZR87_RS07925 at 1684.902 kb on + strand, within ABZR87_RS07925 at 1684.902 kb on + strand, within ABZR87_RS07925 at 1684.903 kb on - strand, within ABZR87_RS07925 at 1684.903 kb on - strand, within ABZR87_RS07925 at 1684.903 kb on - strand, within ABZR87_RS07925 at 1685.055 kb on + strand, within ABZR87_RS07925 at 1685.055 kb on + strand, within ABZR87_RS07925 at 1685.056 kb on - strand, within ABZR87_RS07925 at 1685.056 kb on - strand, within ABZR87_RS07925 at 1685.094 kb on + strand, within ABZR87_RS07925 at 1685.094 kb on + strand, within ABZR87_RS07925 at 1685.094 kb on + strand, within ABZR87_RS07925 at 1685.094 kb on + strand, within ABZR87_RS07925 at 1685.094 kb on + strand, within ABZR87_RS07925 at 1685.095 kb on - strand, within ABZR87_RS07925 at 1685.095 kb on - strand, within ABZR87_RS07925 at 1685.095 kb on - strand, within ABZR87_RS07925 at 1685.095 kb on - strand, within ABZR87_RS07925 at 1685.095 kb on - strand, within ABZR87_RS07925 at 1685.095 kb on - strand, within ABZR87_RS07925 at 1685.190 kb on + strand, within ABZR87_RS07925 at 1685.190 kb on + strand, within ABZR87_RS07925 at 1685.191 kb on - strand, within ABZR87_RS07925 at 1685.191 kb on - strand, within ABZR87_RS07925 at 1685.191 kb on - strand, within ABZR87_RS07925 at 1685.191 kb on - strand, within ABZR87_RS07925 at 1685.224 kb on + strand, within ABZR87_RS07925 at 1685.224 kb on + strand, within ABZR87_RS07925 at 1685.224 kb on + strand, within ABZR87_RS07925 at 1685.224 kb on + strand, within ABZR87_RS07925 at 1685.224 kb on + strand, within ABZR87_RS07925 at 1685.256 kb on + strand, within ABZR87_RS07925 at 1685.256 kb on + strand, within ABZR87_RS07925 at 1685.256 kb on + strand, within ABZR87_RS07925 at 1685.256 kb on + strand, within ABZR87_RS07925 at 1685.256 kb on + strand, within ABZR87_RS07925 at 1685.415 kb on + strand at 1685.415 kb on + strand at 1685.416 kb on - strand at 1685.421 kb on + strand at 1685.422 kb on - strand at 1685.422 kb on - strand at 1685.422 kb on - strand at 1685.430 kb on + strand at 1685.569 kb on + strand at 1685.569 kb on + strand at 1685.570 kb on - strand at 1685.570 kb on - strand at 1685.651 kb on + strand at 1685.679 kb on - strand at 1685.939 kb on + strand at 1685.939 kb on + strand at 1685.939 kb on + strand at 1685.940 kb on - strand at 1685.940 kb on - strand at 1685.978 kb on + strand at 1685.978 kb on + strand at 1685.978 kb on + strand at 1686.002 kb on + strand at 1686.003 kb on - strand at 1686.003 kb on - strand at 1686.003 kb on - strand at 1686.003 kb on - strand at 1686.023 kb on + strand at 1686.087 kb on + strand at 1686.088 kb on - strand at 1686.113 kb on + strand, within ABZR87_RS07930 at 1686.114 kb on - strand, within ABZR87_RS07930 at 1686.179 kb on + strand, within ABZR87_RS07930 at 1686.180 kb on - strand, within ABZR87_RS07930 at 1686.180 kb on - strand, within ABZR87_RS07930 at 1686.180 kb on - strand, within ABZR87_RS07930 at 1686.183 kb on - strand, within ABZR87_RS07930 at 1686.189 kb on - strand, within ABZR87_RS07930 at 1686.189 kb on - strand, within ABZR87_RS07930 at 1686.211 kb on + strand, within ABZR87_RS07930 at 1686.211 kb on + strand, within ABZR87_RS07930 at 1686.272 kb on - strand, within ABZR87_RS07930 at 1686.311 kb on + strand, within ABZR87_RS07930 at 1686.311 kb on + strand, within ABZR87_RS07930 at 1686.311 kb on + strand, within ABZR87_RS07930 at 1686.311 kb on + strand, within ABZR87_RS07930 at 1686.311 kb on + strand, within ABZR87_RS07930 at 1686.312 kb on - strand, within ABZR87_RS07930 at 1686.312 kb on - strand, within ABZR87_RS07930 at 1686.312 kb on - strand, within ABZR87_RS07930 at 1686.312 kb on - strand, within ABZR87_RS07930 at 1686.335 kb on + strand, within ABZR87_RS07930 at 1686.335 kb on + strand, within ABZR87_RS07930 at 1686.336 kb on - strand, within ABZR87_RS07930
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,682,898 + ABZR87_RS07915 0.22 +0.1 1,682,996 + ABZR87_RS07915 0.32 -1.2 1,682,996 + ABZR87_RS07915 0.32 -0.0 1,682,997 - ABZR87_RS07915 0.32 -1.1 1,682,997 - ABZR87_RS07915 0.32 +0.4 1,682,997 - ABZR87_RS07915 0.32 +0.8 1,682,997 - ABZR87_RS07915 0.32 +0.2 1,682,997 - ABZR87_RS07915 0.32 -0.1 1,683,000 + ABZR87_RS07915 0.33 -0.4 1,683,001 - ABZR87_RS07915 0.33 +0.1 1,683,001 - ABZR87_RS07915 0.33 +0.2 1,683,363 + ABZR87_RS07915 0.71 -0.7 1,683,364 - ABZR87_RS07915 0.71 -0.6 1,683,364 - ABZR87_RS07915 0.71 +1.6 1,683,456 + ABZR87_RS07915 0.80 -0.8 1,683,456 + ABZR87_RS07915 0.80 +0.3 1,683,456 + ABZR87_RS07915 0.80 -1.9 1,683,457 - ABZR87_RS07915 0.80 +1.2 1,683,457 - ABZR87_RS07915 0.80 +0.5 1,683,554 + +0.7 1,683,554 + +0.0 1,683,554 + +0.1 1,683,554 + +0.3 1,683,555 - +1.1 1,683,555 - +0.1 1,683,555 - -1.4 1,683,555 - +0.4 1,683,555 - -0.2 1,683,642 + +0.4 1,683,643 - -0.3 1,683,717 - -0.0 1,683,858 + +0.4 1,683,859 - -0.5 1,683,948 + +0.8 1,683,948 + +0.9 1,683,948 + -0.2 1,683,948 + -1.6 1,683,948 + +0.5 1,683,948 + -0.7 1,683,948 + +0.8 1,683,948 + -1.4 1,683,949 - +0.0 1,683,949 - -0.5 1,683,949 - +1.2 1,683,950 + +0.0 1,683,952 + +0.5 1,683,952 + +1.1 1,683,952 + -0.4 1,683,952 + -0.6 1,683,952 + +0.0 1,683,952 + -0.6 1,683,952 + -1.0 1,684,026 + ABZR87_RS07925 0.11 +0.0 1,684,027 - ABZR87_RS07925 0.11 -0.3 1,684,047 + ABZR87_RS07925 0.12 +0.7 1,684,113 + ABZR87_RS07925 0.16 +0.1 1,684,113 + ABZR87_RS07925 0.16 -0.7 1,684,113 + ABZR87_RS07925 0.16 +0.3 1,684,113 + ABZR87_RS07925 0.16 +0.0 1,684,114 - ABZR87_RS07925 0.16 -1.2 1,684,114 - ABZR87_RS07925 0.16 -0.3 1,684,114 - ABZR87_RS07925 0.16 -0.8 1,684,114 - ABZR87_RS07925 0.16 +0.1 1,684,186 - ABZR87_RS07925 0.20 -0.7 1,684,359 + ABZR87_RS07925 0.30 -0.4 1,684,359 + ABZR87_RS07925 0.30 -0.1 1,684,359 + ABZR87_RS07925 0.30 -0.4 1,684,360 - ABZR87_RS07925 0.30 -0.2 1,684,384 + ABZR87_RS07925 0.32 -1.2 1,684,384 + ABZR87_RS07925 0.32 -0.4 1,684,521 + ABZR87_RS07925 0.40 +1.3 1,684,551 + ABZR87_RS07925 0.41 -1.2 1,684,552 - ABZR87_RS07925 0.41 -1.0 1,684,552 - ABZR87_RS07925 0.41 -0.3 1,684,552 - ABZR87_RS07925 0.41 -0.2 1,684,552 - ABZR87_RS07925 0.41 -1.7 1,684,726 - ABZR87_RS07925 0.52 +0.1 1,684,741 - ABZR87_RS07925 0.53 +0.8 1,684,741 - ABZR87_RS07925 0.53 -1.0 1,684,902 + ABZR87_RS07925 0.62 -0.2 1,684,902 + ABZR87_RS07925 0.62 -1.4 1,684,902 + ABZR87_RS07925 0.62 -0.4 1,684,902 + ABZR87_RS07925 0.62 -0.4 1,684,902 + ABZR87_RS07925 0.62 -0.8 1,684,903 - ABZR87_RS07925 0.62 -0.3 1,684,903 - ABZR87_RS07925 0.62 -0.3 1,684,903 - ABZR87_RS07925 0.62 -2.4 1,685,055 + ABZR87_RS07925 0.71 +0.3 1,685,055 + ABZR87_RS07925 0.71 +0.3 1,685,056 - ABZR87_RS07925 0.71 +0.4 1,685,056 - ABZR87_RS07925 0.71 +0.1 1,685,094 + ABZR87_RS07925 0.73 +0.3 1,685,094 + ABZR87_RS07925 0.73 -0.6 1,685,094 + ABZR87_RS07925 0.73 -1.3 1,685,094 + ABZR87_RS07925 0.73 +0.1 1,685,094 + ABZR87_RS07925 0.73 -3.2 1,685,095 - ABZR87_RS07925 0.73 -2.2 1,685,095 - ABZR87_RS07925 0.73 +0.3 1,685,095 - ABZR87_RS07925 0.73 -0.1 1,685,095 - ABZR87_RS07925 0.73 +0.6 1,685,095 - ABZR87_RS07925 0.73 +0.7 1,685,095 - ABZR87_RS07925 0.73 +0.3 1,685,190 + ABZR87_RS07925 0.79 +0.4 1,685,190 + ABZR87_RS07925 0.79 -0.1 1,685,191 - ABZR87_RS07925 0.79 -0.8 1,685,191 - ABZR87_RS07925 0.79 -0.1 1,685,191 - ABZR87_RS07925 0.79 +0.2 1,685,191 - ABZR87_RS07925 0.79 +1.0 1,685,224 + ABZR87_RS07925 0.81 -1.0 1,685,224 + ABZR87_RS07925 0.81 -2.6 1,685,224 + ABZR87_RS07925 0.81 -0.5 1,685,224 + ABZR87_RS07925 0.81 +0.7 1,685,224 + ABZR87_RS07925 0.81 +0.3 1,685,256 + ABZR87_RS07925 0.83 +0.0 1,685,256 + ABZR87_RS07925 0.83 -0.4 1,685,256 + ABZR87_RS07925 0.83 -0.1 1,685,256 + ABZR87_RS07925 0.83 +1.1 1,685,256 + ABZR87_RS07925 0.83 +0.6 1,685,415 + -0.8 1,685,415 + -0.0 1,685,416 - +0.0 1,685,421 + -1.0 1,685,422 - -1.0 1,685,422 - -0.9 1,685,422 - +0.3 1,685,430 + +1.4 1,685,569 + +0.2 1,685,569 + -0.3 1,685,570 - +1.0 1,685,570 - -1.0 1,685,651 + -0.1 1,685,679 - +1.0 1,685,939 + +0.7 1,685,939 + +0.1 1,685,939 + -1.3 1,685,940 - +0.3 1,685,940 - -0.8 1,685,978 + +0.7 1,685,978 + +1.8 1,685,978 + +0.7 1,686,002 + +0.2 1,686,003 - +0.8 1,686,003 - +0.5 1,686,003 - +0.1 1,686,003 - -0.2 1,686,023 + +0.5 1,686,087 + -0.1 1,686,088 - +0.4 1,686,113 + ABZR87_RS07930 0.14 -0.3 1,686,114 - ABZR87_RS07930 0.14 -0.4 1,686,179 + ABZR87_RS07930 0.35 -0.0 1,686,180 - ABZR87_RS07930 0.35 -0.4 1,686,180 - ABZR87_RS07930 0.35 -1.4 1,686,180 - ABZR87_RS07930 0.35 -0.7 1,686,183 - ABZR87_RS07930 0.36 +0.4 1,686,189 - ABZR87_RS07930 0.38 +0.8 1,686,189 - ABZR87_RS07930 0.38 +1.8 1,686,211 + ABZR87_RS07930 0.45 +0.2 1,686,211 + ABZR87_RS07930 0.45 +0.4 1,686,272 - ABZR87_RS07930 0.65 -2.2 1,686,311 + ABZR87_RS07930 0.78 +0.9 1,686,311 + ABZR87_RS07930 0.78 +0.1 1,686,311 + ABZR87_RS07930 0.78 -3.2 1,686,311 + ABZR87_RS07930 0.78 -1.0 1,686,311 + ABZR87_RS07930 0.78 +0.6 1,686,312 - ABZR87_RS07930 0.78 +0.1 1,686,312 - ABZR87_RS07930 0.78 -0.0 1,686,312 - ABZR87_RS07930 0.78 +0.4 1,686,312 - ABZR87_RS07930 0.78 -1.0 1,686,335 + ABZR87_RS07930 0.85 -0.2 1,686,335 + ABZR87_RS07930 0.85 -1.1 1,686,336 - ABZR87_RS07930 0.86 -1.2
Or see this region's nucleotide sequence