Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS06215 and ABZR87_RS06220 are separated by 3 nucleotides ABZR87_RS06220 and ABZR87_RS06225 are separated by 77 nucleotides
ABZR87_RS06215: ABZR87_RS06215 - acyl-CoA dehydrogenase family protein, at 1,326,527 to 1,327,684
_RS06215
ABZR87_RS06220: ABZR87_RS06220 - biotin carboxylase N-terminal domain-containing protein, at 1,327,688 to 1,329,682
_RS06220
ABZR87_RS06225: ABZR87_RS06225 - AMP-binding protein, at 1,329,760 to 1,331,346
_RS06225
Position (kb)
1327
1328
1329
1330 Strain fitness (log2 ratio)
-1
0
1
2 at 1326.719 kb on + strand, within ABZR87_RS06215 at 1326.725 kb on + strand, within ABZR87_RS06215 at 1326.726 kb on - strand, within ABZR87_RS06215 at 1326.968 kb on + strand, within ABZR87_RS06215 at 1326.969 kb on - strand, within ABZR87_RS06215 at 1327.163 kb on + strand, within ABZR87_RS06215 at 1327.164 kb on - strand, within ABZR87_RS06215 at 1327.164 kb on - strand, within ABZR87_RS06215 at 1327.424 kb on + strand, within ABZR87_RS06215 at 1327.425 kb on - strand, within ABZR87_RS06215 at 1327.442 kb on + strand, within ABZR87_RS06215 at 1327.442 kb on + strand, within ABZR87_RS06215 at 1327.442 kb on + strand, within ABZR87_RS06215 at 1327.443 kb on - strand, within ABZR87_RS06215 at 1327.443 kb on - strand, within ABZR87_RS06215 at 1327.443 kb on - strand, within ABZR87_RS06215 at 1327.443 kb on - strand, within ABZR87_RS06215 at 1327.443 kb on - strand, within ABZR87_RS06215 at 1327.580 kb on + strand at 1327.652 kb on + strand at 1327.653 kb on - strand at 1327.653 kb on - strand at 1327.653 kb on - strand at 1327.883 kb on + strand at 1327.884 kb on - strand at 1327.884 kb on - strand at 1327.950 kb on - strand, within ABZR87_RS06220 at 1328.105 kb on + strand, within ABZR87_RS06220 at 1328.106 kb on - strand, within ABZR87_RS06220 at 1328.508 kb on - strand, within ABZR87_RS06220 at 1328.508 kb on - strand, within ABZR87_RS06220 at 1328.508 kb on - strand, within ABZR87_RS06220 at 1328.558 kb on + strand, within ABZR87_RS06220 at 1328.559 kb on - strand, within ABZR87_RS06220 at 1328.852 kb on + strand, within ABZR87_RS06220 at 1328.855 kb on + strand, within ABZR87_RS06220 at 1328.855 kb on + strand, within ABZR87_RS06220 at 1329.182 kb on + strand, within ABZR87_RS06220 at 1329.420 kb on + strand, within ABZR87_RS06220 at 1329.421 kb on + strand, within ABZR87_RS06220 at 1329.428 kb on + strand, within ABZR87_RS06220 at 1329.429 kb on - strand, within ABZR87_RS06220 at 1329.429 kb on - strand, within ABZR87_RS06220 at 1329.429 kb on - strand, within ABZR87_RS06220 at 1329.429 kb on - strand, within ABZR87_RS06220 at 1329.429 kb on - strand, within ABZR87_RS06220 at 1329.825 kb on + strand at 1329.826 kb on - strand at 1329.826 kb on - strand at 1329.926 kb on - strand, within ABZR87_RS06225 at 1330.016 kb on - strand, within ABZR87_RS06225 at 1330.084 kb on + strand, within ABZR87_RS06225 at 1330.085 kb on - strand, within ABZR87_RS06225 at 1330.085 kb on - strand, within ABZR87_RS06225 at 1330.085 kb on - strand, within ABZR87_RS06225 at 1330.136 kb on - strand, within ABZR87_RS06225 at 1330.251 kb on + strand, within ABZR87_RS06225 at 1330.301 kb on - strand, within ABZR87_RS06225 at 1330.301 kb on - strand, within ABZR87_RS06225 at 1330.301 kb on - strand, within ABZR87_RS06225 at 1330.301 kb on - strand, within ABZR87_RS06225 at 1330.301 kb on - strand, within ABZR87_RS06225 at 1330.577 kb on - strand, within ABZR87_RS06225 at 1330.577 kb on - strand, within ABZR87_RS06225
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,326,719 + ABZR87_RS06215 0.17 -0.7 1,326,725 + ABZR87_RS06215 0.17 -1.2 1,326,726 - ABZR87_RS06215 0.17 +0.1 1,326,968 + ABZR87_RS06215 0.38 +0.6 1,326,969 - ABZR87_RS06215 0.38 +0.9 1,327,163 + ABZR87_RS06215 0.55 +1.3 1,327,164 - ABZR87_RS06215 0.55 +0.5 1,327,164 - ABZR87_RS06215 0.55 -0.4 1,327,424 + ABZR87_RS06215 0.77 -0.4 1,327,425 - ABZR87_RS06215 0.78 -0.8 1,327,442 + ABZR87_RS06215 0.79 -1.4 1,327,442 + ABZR87_RS06215 0.79 -0.1 1,327,442 + ABZR87_RS06215 0.79 -1.0 1,327,443 - ABZR87_RS06215 0.79 -0.6 1,327,443 - ABZR87_RS06215 0.79 +0.8 1,327,443 - ABZR87_RS06215 0.79 -0.7 1,327,443 - ABZR87_RS06215 0.79 -0.2 1,327,443 - ABZR87_RS06215 0.79 -0.3 1,327,580 + -0.0 1,327,652 + +0.7 1,327,653 - +0.3 1,327,653 - +0.6 1,327,653 - -0.1 1,327,883 + +0.1 1,327,884 - -0.6 1,327,884 - -1.3 1,327,950 - ABZR87_RS06220 0.13 -1.0 1,328,105 + ABZR87_RS06220 0.21 -0.6 1,328,106 - ABZR87_RS06220 0.21 +0.2 1,328,508 - ABZR87_RS06220 0.41 -1.2 1,328,508 - ABZR87_RS06220 0.41 -0.4 1,328,508 - ABZR87_RS06220 0.41 +1.5 1,328,558 + ABZR87_RS06220 0.44 +0.6 1,328,559 - ABZR87_RS06220 0.44 +0.1 1,328,852 + ABZR87_RS06220 0.58 +0.7 1,328,855 + ABZR87_RS06220 0.58 -1.3 1,328,855 + ABZR87_RS06220 0.58 -0.1 1,329,182 + ABZR87_RS06220 0.75 +1.0 1,329,420 + ABZR87_RS06220 0.87 +0.4 1,329,421 + ABZR87_RS06220 0.87 -0.7 1,329,428 + ABZR87_RS06220 0.87 -0.2 1,329,429 - ABZR87_RS06220 0.87 +0.6 1,329,429 - ABZR87_RS06220 0.87 -0.4 1,329,429 - ABZR87_RS06220 0.87 -0.2 1,329,429 - ABZR87_RS06220 0.87 -0.0 1,329,429 - ABZR87_RS06220 0.87 +0.3 1,329,825 + -0.1 1,329,826 - +0.5 1,329,826 - -0.2 1,329,926 - ABZR87_RS06225 0.10 +2.7 1,330,016 - ABZR87_RS06225 0.16 +0.5 1,330,084 + ABZR87_RS06225 0.20 +0.2 1,330,085 - ABZR87_RS06225 0.20 -1.2 1,330,085 - ABZR87_RS06225 0.20 +0.8 1,330,085 - ABZR87_RS06225 0.20 -0.3 1,330,136 - ABZR87_RS06225 0.24 -0.3 1,330,251 + ABZR87_RS06225 0.31 -0.5 1,330,301 - ABZR87_RS06225 0.34 -0.1 1,330,301 - ABZR87_RS06225 0.34 -0.7 1,330,301 - ABZR87_RS06225 0.34 +0.1 1,330,301 - ABZR87_RS06225 0.34 -1.4 1,330,301 - ABZR87_RS06225 0.34 +0.7 1,330,577 - ABZR87_RS06225 0.51 -0.4 1,330,577 - ABZR87_RS06225 0.51 +0.3
Or see this region's nucleotide sequence