Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS05945 and ABZR87_RS05950 are separated by 73 nucleotides ABZR87_RS05950 and ABZR87_RS05955 are separated by 57 nucleotides ABZR87_RS05955 and ABZR87_RS05960 are separated by 22 nucleotides
ABZR87_RS05945: ABZR87_RS05945 - hypothetical protein, at 1,271,346 to 1,271,924
_RS05945
ABZR87_RS05950: ABZR87_RS05950 - nucleotidyltransferase domain-containing protein, at 1,271,998 to 1,272,807
_RS05950
ABZR87_RS05955: ABZR87_RS05955 - RNA 3'-terminal phosphate cyclase, at 1,272,865 to 1,273,932
_RS05955
ABZR87_RS05960: ABZR87_RS05960 - thiamine pyrophosphate-requiring protein, at 1,273,955 to 1,275,751
_RS05960
Position (kb)
1272
1273
1274 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1271.888 kb on + strand at 1271.889 kb on - strand at 1271.889 kb on - strand at 1271.889 kb on - strand at 1271.958 kb on + strand at 1271.958 kb on + strand at 1271.959 kb on - strand at 1271.959 kb on - strand at 1271.959 kb on - strand at 1271.998 kb on - strand at 1271.998 kb on - strand at 1272.010 kb on + strand at 1272.010 kb on + strand at 1272.010 kb on + strand at 1272.011 kb on - strand at 1272.011 kb on - strand at 1272.011 kb on - strand at 1272.169 kb on + strand, within ABZR87_RS05950 at 1272.170 kb on - strand, within ABZR87_RS05950 at 1272.190 kb on + strand, within ABZR87_RS05950 at 1272.190 kb on + strand, within ABZR87_RS05950 at 1272.190 kb on + strand, within ABZR87_RS05950 at 1272.190 kb on + strand, within ABZR87_RS05950 at 1272.191 kb on - strand, within ABZR87_RS05950 at 1272.191 kb on - strand, within ABZR87_RS05950 at 1272.191 kb on - strand, within ABZR87_RS05950 at 1272.191 kb on - strand, within ABZR87_RS05950 at 1272.191 kb on - strand, within ABZR87_RS05950 at 1272.338 kb on - strand, within ABZR87_RS05950 at 1272.338 kb on - strand, within ABZR87_RS05950 at 1272.338 kb on - strand, within ABZR87_RS05950 at 1272.418 kb on + strand, within ABZR87_RS05950 at 1272.418 kb on + strand, within ABZR87_RS05950 at 1272.418 kb on + strand, within ABZR87_RS05950 at 1272.418 kb on + strand, within ABZR87_RS05950 at 1272.418 kb on + strand, within ABZR87_RS05950 at 1272.418 kb on + strand, within ABZR87_RS05950 at 1272.418 kb on + strand, within ABZR87_RS05950 at 1272.419 kb on - strand, within ABZR87_RS05950 at 1272.419 kb on - strand, within ABZR87_RS05950 at 1272.419 kb on - strand, within ABZR87_RS05950 at 1272.484 kb on + strand, within ABZR87_RS05950 at 1272.485 kb on - strand, within ABZR87_RS05950 at 1272.485 kb on - strand, within ABZR87_RS05950 at 1272.485 kb on - strand, within ABZR87_RS05950 at 1272.490 kb on + strand, within ABZR87_RS05950 at 1272.490 kb on + strand, within ABZR87_RS05950 at 1272.490 kb on + strand, within ABZR87_RS05950 at 1272.490 kb on + strand, within ABZR87_RS05950 at 1272.490 kb on - strand, within ABZR87_RS05950 at 1272.491 kb on - strand, within ABZR87_RS05950 at 1272.491 kb on - strand, within ABZR87_RS05950 at 1272.491 kb on - strand, within ABZR87_RS05950 at 1272.491 kb on - strand, within ABZR87_RS05950 at 1272.491 kb on - strand, within ABZR87_RS05950 at 1272.491 kb on - strand, within ABZR87_RS05950 at 1272.491 kb on - strand, within ABZR87_RS05950 at 1272.652 kb on + strand, within ABZR87_RS05950 at 1272.652 kb on + strand, within ABZR87_RS05950 at 1272.653 kb on - strand, within ABZR87_RS05950 at 1272.798 kb on + strand at 1272.798 kb on + strand at 1272.799 kb on - strand at 1272.799 kb on - strand at 1272.875 kb on + strand at 1272.875 kb on + strand at 1272.878 kb on + strand at 1272.878 kb on + strand at 1272.878 kb on + strand at 1272.878 kb on + strand at 1272.879 kb on - strand at 1272.879 kb on - strand at 1273.171 kb on + strand, within ABZR87_RS05955 at 1273.172 kb on - strand, within ABZR87_RS05955 at 1273.387 kb on + strand, within ABZR87_RS05955 at 1273.410 kb on - strand, within ABZR87_RS05955 at 1273.410 kb on - strand, within ABZR87_RS05955 at 1273.451 kb on + strand, within ABZR87_RS05955 at 1273.452 kb on - strand, within ABZR87_RS05955 at 1273.730 kb on - strand, within ABZR87_RS05955 at 1273.760 kb on - strand, within ABZR87_RS05955 at 1274.043 kb on + strand at 1274.123 kb on + strand at 1274.199 kb on + strand, within ABZR87_RS05960 at 1274.199 kb on + strand, within ABZR87_RS05960 at 1274.199 kb on + strand, within ABZR87_RS05960 at 1274.199 kb on + strand, within ABZR87_RS05960 at 1274.199 kb on + strand, within ABZR87_RS05960 at 1274.199 kb on + strand, within ABZR87_RS05960 at 1274.200 kb on - strand, within ABZR87_RS05960 at 1274.200 kb on - strand, within ABZR87_RS05960 at 1274.200 kb on - strand, within ABZR87_RS05960 at 1274.200 kb on - strand, within ABZR87_RS05960 at 1274.238 kb on + strand, within ABZR87_RS05960 at 1274.376 kb on + strand, within ABZR87_RS05960 at 1274.377 kb on - strand, within ABZR87_RS05960 at 1274.377 kb on - strand, within ABZR87_RS05960 at 1274.898 kb on + strand, within ABZR87_RS05960 at 1274.898 kb on + strand, within ABZR87_RS05960 at 1274.899 kb on - strand, within ABZR87_RS05960
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,271,888 + +0.8 1,271,889 - -0.1 1,271,889 - +1.0 1,271,889 - -0.3 1,271,958 + +1.1 1,271,958 + +1.9 1,271,959 - +0.8 1,271,959 - -0.1 1,271,959 - -0.8 1,271,998 - +1.7 1,271,998 - +1.0 1,272,010 + -0.4 1,272,010 + +0.1 1,272,010 + -0.1 1,272,011 - -2.6 1,272,011 - +0.2 1,272,011 - +1.4 1,272,169 + ABZR87_RS05950 0.21 -0.1 1,272,170 - ABZR87_RS05950 0.21 +0.0 1,272,190 + ABZR87_RS05950 0.24 -0.3 1,272,190 + ABZR87_RS05950 0.24 -0.1 1,272,190 + ABZR87_RS05950 0.24 -0.3 1,272,190 + ABZR87_RS05950 0.24 -0.6 1,272,191 - ABZR87_RS05950 0.24 -0.4 1,272,191 - ABZR87_RS05950 0.24 +0.1 1,272,191 - ABZR87_RS05950 0.24 +0.1 1,272,191 - ABZR87_RS05950 0.24 +0.2 1,272,191 - ABZR87_RS05950 0.24 -0.3 1,272,338 - ABZR87_RS05950 0.42 +0.4 1,272,338 - ABZR87_RS05950 0.42 -0.1 1,272,338 - ABZR87_RS05950 0.42 +0.1 1,272,418 + ABZR87_RS05950 0.52 -0.1 1,272,418 + ABZR87_RS05950 0.52 -0.5 1,272,418 + ABZR87_RS05950 0.52 +0.2 1,272,418 + ABZR87_RS05950 0.52 -0.3 1,272,418 + ABZR87_RS05950 0.52 -0.1 1,272,418 + ABZR87_RS05950 0.52 -0.1 1,272,418 + ABZR87_RS05950 0.52 +0.9 1,272,419 - ABZR87_RS05950 0.52 -0.0 1,272,419 - ABZR87_RS05950 0.52 -1.8 1,272,419 - ABZR87_RS05950 0.52 +0.6 1,272,484 + ABZR87_RS05950 0.60 -0.2 1,272,485 - ABZR87_RS05950 0.60 -0.1 1,272,485 - ABZR87_RS05950 0.60 -0.9 1,272,485 - ABZR87_RS05950 0.60 -0.2 1,272,490 + ABZR87_RS05950 0.61 -0.0 1,272,490 + ABZR87_RS05950 0.61 +0.6 1,272,490 + ABZR87_RS05950 0.61 +0.7 1,272,490 + ABZR87_RS05950 0.61 -1.1 1,272,490 - ABZR87_RS05950 0.61 +0.2 1,272,491 - ABZR87_RS05950 0.61 +0.8 1,272,491 - ABZR87_RS05950 0.61 -0.3 1,272,491 - ABZR87_RS05950 0.61 -0.6 1,272,491 - ABZR87_RS05950 0.61 +0.2 1,272,491 - ABZR87_RS05950 0.61 +0.6 1,272,491 - ABZR87_RS05950 0.61 +1.1 1,272,491 - ABZR87_RS05950 0.61 +0.5 1,272,652 + ABZR87_RS05950 0.81 -0.4 1,272,652 + ABZR87_RS05950 0.81 +0.0 1,272,653 - ABZR87_RS05950 0.81 +0.1 1,272,798 + -0.7 1,272,798 + +0.9 1,272,799 - +0.2 1,272,799 - +0.4 1,272,875 + +0.9 1,272,875 + +0.2 1,272,878 + -0.5 1,272,878 + +0.3 1,272,878 + -0.2 1,272,878 + -0.4 1,272,879 - +0.8 1,272,879 - +0.1 1,273,171 + ABZR87_RS05955 0.29 -0.2 1,273,172 - ABZR87_RS05955 0.29 +0.1 1,273,387 + ABZR87_RS05955 0.49 -1.6 1,273,410 - ABZR87_RS05955 0.51 +0.1 1,273,410 - ABZR87_RS05955 0.51 -0.9 1,273,451 + ABZR87_RS05955 0.55 -0.5 1,273,452 - ABZR87_RS05955 0.55 +0.2 1,273,730 - ABZR87_RS05955 0.81 -0.3 1,273,760 - ABZR87_RS05955 0.84 -0.6 1,274,043 + +0.1 1,274,123 + +0.1 1,274,199 + ABZR87_RS05960 0.14 +0.2 1,274,199 + ABZR87_RS05960 0.14 +0.5 1,274,199 + ABZR87_RS05960 0.14 +0.6 1,274,199 + ABZR87_RS05960 0.14 +0.0 1,274,199 + ABZR87_RS05960 0.14 +1.1 1,274,199 + ABZR87_RS05960 0.14 +0.3 1,274,200 - ABZR87_RS05960 0.14 -0.6 1,274,200 - ABZR87_RS05960 0.14 -0.7 1,274,200 - ABZR87_RS05960 0.14 +0.1 1,274,200 - ABZR87_RS05960 0.14 -2.2 1,274,238 + ABZR87_RS05960 0.16 -0.1 1,274,376 + ABZR87_RS05960 0.23 +0.5 1,274,377 - ABZR87_RS05960 0.23 +0.5 1,274,377 - ABZR87_RS05960 0.23 +0.8 1,274,898 + ABZR87_RS05960 0.52 -1.7 1,274,898 + ABZR87_RS05960 0.52 -2.2 1,274,899 - ABZR87_RS05960 0.53 +0.8
Or see this region's nucleotide sequence