Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS05330 and ABZR87_RS05335 are separated by 338 nucleotides ABZR87_RS05335 and ABZR87_RS05340 are separated by 15 nucleotides ABZR87_RS05340 and ABZR87_RS05345 are separated by 4 nucleotides ABZR87_RS05345 and ABZR87_RS05350 are separated by 7 nucleotides
ABZR87_RS05330: ABZR87_RS05330 - 30S ribosomal protein S21, at 1,124,885 to 1,125,097
_RS05330
ABZR87_RS05335: ABZR87_RS05335 - hypothetical protein, at 1,125,436 to 1,125,690
_RS05335
ABZR87_RS05340: ABZR87_RS05340 - low molecular weight protein tyrosine phosphatase family protein, at 1,125,706 to 1,126,029
_RS05340
ABZR87_RS05345: ABZR87_RS05345 - NUDIX hydrolase, at 1,126,034 to 1,126,423
_RS05345
ABZR87_RS05350: ABZR87_RS05350 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE, at 1,126,431 to 1,127,876
_RS05350
Position (kb)
1126
1127 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1125.092 kb on + strand at 1125.092 kb on + strand at 1125.092 kb on + strand at 1125.092 kb on + strand at 1125.092 kb on + strand at 1125.093 kb on - strand at 1125.093 kb on - strand at 1125.093 kb on - strand at 1125.093 kb on - strand at 1125.095 kb on + strand at 1125.095 kb on + strand at 1125.095 kb on + strand at 1125.096 kb on - strand at 1125.096 kb on - strand at 1125.244 kb on - strand at 1125.244 kb on - strand at 1125.244 kb on - strand at 1125.252 kb on + strand at 1125.252 kb on + strand at 1125.252 kb on + strand at 1125.252 kb on + strand at 1125.253 kb on - strand at 1125.253 kb on - strand at 1125.346 kb on - strand at 1125.395 kb on + strand at 1125.395 kb on + strand at 1125.395 kb on + strand at 1125.395 kb on + strand at 1125.401 kb on - strand at 1125.441 kb on + strand at 1125.441 kb on + strand at 1125.441 kb on + strand at 1125.441 kb on + strand at 1125.442 kb on - strand at 1125.442 kb on - strand at 1125.442 kb on - strand at 1125.442 kb on - strand at 1125.442 kb on - strand at 1125.444 kb on + strand at 1125.444 kb on + strand at 1125.444 kb on + strand at 1125.445 kb on - strand at 1125.445 kb on - strand at 1125.445 kb on - strand at 1125.445 kb on - strand at 1125.445 kb on - strand at 1125.460 kb on + strand at 1125.460 kb on + strand at 1125.461 kb on - strand at 1125.461 kb on - strand at 1125.461 kb on - strand at 1125.461 kb on - strand at 1125.482 kb on - strand, within ABZR87_RS05335 at 1125.538 kb on + strand, within ABZR87_RS05335 at 1125.538 kb on + strand, within ABZR87_RS05335 at 1125.538 kb on + strand, within ABZR87_RS05335 at 1125.538 kb on + strand, within ABZR87_RS05335 at 1125.538 kb on + strand, within ABZR87_RS05335 at 1125.538 kb on + strand, within ABZR87_RS05335 at 1125.538 kb on + strand, within ABZR87_RS05335 at 1125.538 kb on + strand, within ABZR87_RS05335 at 1125.538 kb on + strand, within ABZR87_RS05335 at 1125.538 kb on + strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.539 kb on - strand, within ABZR87_RS05335 at 1125.545 kb on + strand, within ABZR87_RS05335 at 1125.607 kb on + strand, within ABZR87_RS05335 at 1125.607 kb on + strand, within ABZR87_RS05335 at 1125.607 kb on + strand, within ABZR87_RS05335 at 1125.607 kb on + strand, within ABZR87_RS05335 at 1125.608 kb on - strand, within ABZR87_RS05335 at 1125.707 kb on + strand at 1125.708 kb on - strand at 1125.708 kb on - strand at 1125.773 kb on + strand, within ABZR87_RS05340 at 1125.773 kb on + strand, within ABZR87_RS05340 at 1125.773 kb on + strand, within ABZR87_RS05340 at 1125.773 kb on + strand, within ABZR87_RS05340 at 1125.773 kb on + strand, within ABZR87_RS05340 at 1125.773 kb on + strand, within ABZR87_RS05340 at 1125.773 kb on + strand, within ABZR87_RS05340 at 1125.773 kb on + strand, within ABZR87_RS05340 at 1125.774 kb on - strand, within ABZR87_RS05340 at 1125.774 kb on - strand, within ABZR87_RS05340 at 1125.774 kb on - strand, within ABZR87_RS05340 at 1125.777 kb on + strand, within ABZR87_RS05340 at 1125.777 kb on + strand, within ABZR87_RS05340 at 1125.778 kb on - strand, within ABZR87_RS05340 at 1125.787 kb on - strand, within ABZR87_RS05340 at 1125.787 kb on - strand, within ABZR87_RS05340 at 1125.814 kb on + strand, within ABZR87_RS05340 at 1125.910 kb on + strand, within ABZR87_RS05340 at 1125.910 kb on + strand, within ABZR87_RS05340 at 1125.910 kb on + strand, within ABZR87_RS05340 at 1125.910 kb on + strand, within ABZR87_RS05340 at 1125.910 kb on + strand, within ABZR87_RS05340 at 1125.910 kb on + strand, within ABZR87_RS05340 at 1125.910 kb on + strand, within ABZR87_RS05340 at 1125.911 kb on - strand, within ABZR87_RS05340 at 1125.911 kb on - strand, within ABZR87_RS05340 at 1125.911 kb on - strand, within ABZR87_RS05340 at 1125.911 kb on - strand, within ABZR87_RS05340 at 1125.911 kb on - strand, within ABZR87_RS05340 at 1125.990 kb on + strand, within ABZR87_RS05340 at 1125.991 kb on - strand, within ABZR87_RS05340 at 1125.991 kb on - strand, within ABZR87_RS05340 at 1125.994 kb on + strand, within ABZR87_RS05340 at 1125.994 kb on + strand, within ABZR87_RS05340 at 1126.035 kb on + strand at 1126.035 kb on + strand at 1126.035 kb on + strand at 1126.035 kb on + strand at 1126.036 kb on - strand at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.179 kb on + strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.180 kb on - strand, within ABZR87_RS05345 at 1126.189 kb on + strand, within ABZR87_RS05345 at 1126.190 kb on - strand, within ABZR87_RS05345 at 1126.190 kb on - strand, within ABZR87_RS05345 at 1126.190 kb on - strand, within ABZR87_RS05345 at 1126.190 kb on - strand, within ABZR87_RS05345 at 1126.190 kb on - strand, within ABZR87_RS05345 at 1126.224 kb on + strand, within ABZR87_RS05345 at 1126.224 kb on + strand, within ABZR87_RS05345 at 1126.224 kb on + strand, within ABZR87_RS05345 at 1126.224 kb on + strand, within ABZR87_RS05345 at 1126.224 kb on + strand, within ABZR87_RS05345 at 1126.224 kb on + strand, within ABZR87_RS05345 at 1126.224 kb on + strand, within ABZR87_RS05345 at 1126.224 kb on + strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.225 kb on - strand, within ABZR87_RS05345 at 1126.358 kb on + strand, within ABZR87_RS05345 at 1126.358 kb on + strand, within ABZR87_RS05345 at 1126.413 kb on + strand at 1126.442 kb on + strand at 1126.443 kb on - strand at 1126.496 kb on + strand at 1126.643 kb on - strand, within ABZR87_RS05350 at 1127.022 kb on - strand, within ABZR87_RS05350
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,125,092 + +1.7 1,125,092 + +0.0 1,125,092 + -0.1 1,125,092 + -0.2 1,125,092 + -1.6 1,125,093 - -0.3 1,125,093 - -0.1 1,125,093 - -0.3 1,125,093 - +0.1 1,125,095 + -1.6 1,125,095 + -1.3 1,125,095 + -1.2 1,125,096 - +0.5 1,125,096 - +0.3 1,125,244 - +0.2 1,125,244 - +1.2 1,125,244 - -2.1 1,125,252 + +0.2 1,125,252 + -1.1 1,125,252 + -1.7 1,125,252 + -0.4 1,125,253 - -0.6 1,125,253 - -0.9 1,125,346 - +1.1 1,125,395 + -0.6 1,125,395 + -1.0 1,125,395 + -0.4 1,125,395 + +0.0 1,125,401 - +0.6 1,125,441 + -0.8 1,125,441 + -0.3 1,125,441 + +2.7 1,125,441 + -0.2 1,125,442 - -0.5 1,125,442 - -0.5 1,125,442 - -0.4 1,125,442 - +1.8 1,125,442 - -0.6 1,125,444 + +0.1 1,125,444 + +1.7 1,125,444 + +0.7 1,125,445 - -0.6 1,125,445 - -0.6 1,125,445 - +1.2 1,125,445 - -0.1 1,125,445 - -0.3 1,125,460 + -0.6 1,125,460 + -0.3 1,125,461 - +0.5 1,125,461 - -3.1 1,125,461 - -0.7 1,125,461 - +0.8 1,125,482 - ABZR87_RS05335 0.18 +1.2 1,125,538 + ABZR87_RS05335 0.40 +0.1 1,125,538 + ABZR87_RS05335 0.40 +0.4 1,125,538 + ABZR87_RS05335 0.40 -1.1 1,125,538 + ABZR87_RS05335 0.40 -0.7 1,125,538 + ABZR87_RS05335 0.40 -1.3 1,125,538 + ABZR87_RS05335 0.40 -0.8 1,125,538 + ABZR87_RS05335 0.40 -0.5 1,125,538 + ABZR87_RS05335 0.40 +0.2 1,125,538 + ABZR87_RS05335 0.40 +0.6 1,125,538 + ABZR87_RS05335 0.40 +0.3 1,125,539 - ABZR87_RS05335 0.40 +0.6 1,125,539 - ABZR87_RS05335 0.40 +0.3 1,125,539 - ABZR87_RS05335 0.40 +0.3 1,125,539 - ABZR87_RS05335 0.40 -0.6 1,125,539 - ABZR87_RS05335 0.40 -0.3 1,125,539 - ABZR87_RS05335 0.40 +0.4 1,125,539 - ABZR87_RS05335 0.40 +0.6 1,125,539 - ABZR87_RS05335 0.40 -0.8 1,125,539 - ABZR87_RS05335 0.40 -0.5 1,125,539 - ABZR87_RS05335 0.40 -0.5 1,125,539 - ABZR87_RS05335 0.40 -0.6 1,125,545 + ABZR87_RS05335 0.43 +1.2 1,125,607 + ABZR87_RS05335 0.67 -0.1 1,125,607 + ABZR87_RS05335 0.67 +0.3 1,125,607 + ABZR87_RS05335 0.67 -0.3 1,125,607 + ABZR87_RS05335 0.67 -1.4 1,125,608 - ABZR87_RS05335 0.67 -0.2 1,125,707 + +0.0 1,125,708 - -0.4 1,125,708 - +0.5 1,125,773 + ABZR87_RS05340 0.21 +0.4 1,125,773 + ABZR87_RS05340 0.21 -0.4 1,125,773 + ABZR87_RS05340 0.21 +0.1 1,125,773 + ABZR87_RS05340 0.21 -0.3 1,125,773 + ABZR87_RS05340 0.21 +0.2 1,125,773 + ABZR87_RS05340 0.21 -0.7 1,125,773 + ABZR87_RS05340 0.21 -0.1 1,125,773 + ABZR87_RS05340 0.21 +0.1 1,125,774 - ABZR87_RS05340 0.21 +0.7 1,125,774 - ABZR87_RS05340 0.21 +0.2 1,125,774 - ABZR87_RS05340 0.21 -0.4 1,125,777 + ABZR87_RS05340 0.22 -0.5 1,125,777 + ABZR87_RS05340 0.22 -0.2 1,125,778 - ABZR87_RS05340 0.22 +0.7 1,125,787 - ABZR87_RS05340 0.25 -0.5 1,125,787 - ABZR87_RS05340 0.25 +0.3 1,125,814 + ABZR87_RS05340 0.33 -1.0 1,125,910 + ABZR87_RS05340 0.63 +1.7 1,125,910 + ABZR87_RS05340 0.63 +0.1 1,125,910 + ABZR87_RS05340 0.63 +0.1 1,125,910 + ABZR87_RS05340 0.63 -0.9 1,125,910 + ABZR87_RS05340 0.63 -0.7 1,125,910 + ABZR87_RS05340 0.63 +1.0 1,125,910 + ABZR87_RS05340 0.63 -1.3 1,125,911 - ABZR87_RS05340 0.63 -0.6 1,125,911 - ABZR87_RS05340 0.63 +0.9 1,125,911 - ABZR87_RS05340 0.63 -0.0 1,125,911 - ABZR87_RS05340 0.63 +1.3 1,125,911 - ABZR87_RS05340 0.63 +1.0 1,125,990 + ABZR87_RS05340 0.88 +1.5 1,125,991 - ABZR87_RS05340 0.88 -0.9 1,125,991 - ABZR87_RS05340 0.88 -0.9 1,125,994 + ABZR87_RS05340 0.89 +0.3 1,125,994 + ABZR87_RS05340 0.89 +0.3 1,126,035 + -0.0 1,126,035 + -1.1 1,126,035 + -1.0 1,126,035 + -0.6 1,126,036 - +0.2 1,126,179 + ABZR87_RS05345 0.37 +0.5 1,126,179 + ABZR87_RS05345 0.37 +0.1 1,126,179 + ABZR87_RS05345 0.37 -0.3 1,126,179 + ABZR87_RS05345 0.37 +0.4 1,126,179 + ABZR87_RS05345 0.37 -0.8 1,126,179 + ABZR87_RS05345 0.37 -1.1 1,126,179 + ABZR87_RS05345 0.37 +0.6 1,126,179 + ABZR87_RS05345 0.37 -0.2 1,126,179 + ABZR87_RS05345 0.37 +0.7 1,126,179 + ABZR87_RS05345 0.37 +0.3 1,126,179 + ABZR87_RS05345 0.37 -0.2 1,126,179 + ABZR87_RS05345 0.37 -0.4 1,126,179 + ABZR87_RS05345 0.37 +0.2 1,126,179 + ABZR87_RS05345 0.37 +0.5 1,126,180 - ABZR87_RS05345 0.37 -0.4 1,126,180 - ABZR87_RS05345 0.37 -0.9 1,126,180 - ABZR87_RS05345 0.37 -0.1 1,126,180 - ABZR87_RS05345 0.37 +0.4 1,126,180 - ABZR87_RS05345 0.37 +0.2 1,126,180 - ABZR87_RS05345 0.37 -2.0 1,126,180 - ABZR87_RS05345 0.37 -0.8 1,126,180 - ABZR87_RS05345 0.37 +0.1 1,126,180 - ABZR87_RS05345 0.37 -0.6 1,126,180 - ABZR87_RS05345 0.37 +0.1 1,126,180 - ABZR87_RS05345 0.37 +0.1 1,126,189 + ABZR87_RS05345 0.40 +0.5 1,126,190 - ABZR87_RS05345 0.40 +0.0 1,126,190 - ABZR87_RS05345 0.40 +0.8 1,126,190 - ABZR87_RS05345 0.40 -0.0 1,126,190 - ABZR87_RS05345 0.40 -1.3 1,126,190 - ABZR87_RS05345 0.40 -0.2 1,126,224 + ABZR87_RS05345 0.49 -0.1 1,126,224 + ABZR87_RS05345 0.49 +0.8 1,126,224 + ABZR87_RS05345 0.49 -1.0 1,126,224 + ABZR87_RS05345 0.49 -0.6 1,126,224 + ABZR87_RS05345 0.49 -0.5 1,126,224 + ABZR87_RS05345 0.49 +0.9 1,126,224 + ABZR87_RS05345 0.49 -0.1 1,126,224 + ABZR87_RS05345 0.49 +0.8 1,126,225 - ABZR87_RS05345 0.49 -0.0 1,126,225 - ABZR87_RS05345 0.49 -0.2 1,126,225 - ABZR87_RS05345 0.49 -0.6 1,126,225 - ABZR87_RS05345 0.49 +0.4 1,126,225 - ABZR87_RS05345 0.49 +0.2 1,126,225 - ABZR87_RS05345 0.49 +0.9 1,126,225 - ABZR87_RS05345 0.49 -0.3 1,126,225 - ABZR87_RS05345 0.49 -0.2 1,126,225 - ABZR87_RS05345 0.49 -0.8 1,126,225 - ABZR87_RS05345 0.49 -0.9 1,126,225 - ABZR87_RS05345 0.49 -0.4 1,126,225 - ABZR87_RS05345 0.49 -0.9 1,126,225 - ABZR87_RS05345 0.49 +0.4 1,126,358 + ABZR87_RS05345 0.83 -1.3 1,126,358 + ABZR87_RS05345 0.83 -0.9 1,126,413 + +0.4 1,126,442 + -1.1 1,126,443 - -0.3 1,126,496 + -1.9 1,126,643 - ABZR87_RS05350 0.15 -0.8 1,127,022 - ABZR87_RS05350 0.41 -3.6
Or see this region's nucleotide sequence