Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS05120 and ABZR87_RS05125 are separated by 214 nucleotides ABZR87_RS05125 and ABZR87_RS05130 are separated by 97 nucleotides ABZR87_RS05130 and ABZR87_RS05135 are separated by 33 nucleotides ABZR87_RS05135 and ABZR87_RS05140 are separated by 47 nucleotides
ABZR87_RS05120: ABZR87_RS05120 - ATP-dependent protease ATPase subunit HslU, at 1,079,035 to 1,080,366
_RS05120
ABZR87_RS05125: ABZR87_RS05125 - hypothetical protein, at 1,080,581 to 1,081,195
_RS05125
ABZR87_RS05130: ABZR87_RS05130 - CsbD family protein, at 1,081,293 to 1,081,496
_RS05130
ABZR87_RS05135: ABZR87_RS05135 - response regulator transcription factor, at 1,081,530 to 1,082,108
_RS05135
ABZR87_RS05140: ABZR87_RS05140 - ATP-binding protein, at 1,082,156 to 1,083,502
_RS05140
Position (kb)
1081
1082 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1080.304 kb on + strand at 1080.305 kb on - strand at 1080.305 kb on - strand at 1080.355 kb on + strand at 1080.356 kb on - strand at 1080.356 kb on - strand at 1080.372 kb on + strand at 1080.372 kb on + strand at 1080.373 kb on - strand at 1080.373 kb on - strand at 1080.373 kb on - strand at 1080.373 kb on - strand at 1080.388 kb on + strand at 1080.388 kb on + strand at 1080.389 kb on - strand at 1080.389 kb on - strand at 1080.495 kb on - strand at 1080.580 kb on + strand at 1080.580 kb on + strand at 1080.580 kb on + strand at 1080.580 kb on + strand at 1080.581 kb on - strand at 1080.581 kb on - strand at 1080.581 kb on - strand at 1080.716 kb on + strand, within ABZR87_RS05125 at 1080.716 kb on + strand, within ABZR87_RS05125 at 1080.716 kb on + strand, within ABZR87_RS05125 at 1080.716 kb on + strand, within ABZR87_RS05125 at 1080.717 kb on - strand, within ABZR87_RS05125 at 1080.749 kb on + strand, within ABZR87_RS05125 at 1080.749 kb on + strand, within ABZR87_RS05125 at 1080.749 kb on + strand, within ABZR87_RS05125 at 1080.750 kb on - strand, within ABZR87_RS05125 at 1080.750 kb on - strand, within ABZR87_RS05125 at 1080.750 kb on - strand, within ABZR87_RS05125 at 1080.750 kb on - strand, within ABZR87_RS05125 at 1080.827 kb on + strand, within ABZR87_RS05125 at 1080.827 kb on + strand, within ABZR87_RS05125 at 1080.827 kb on + strand, within ABZR87_RS05125 at 1080.827 kb on + strand, within ABZR87_RS05125 at 1080.828 kb on - strand, within ABZR87_RS05125 at 1080.828 kb on - strand, within ABZR87_RS05125 at 1080.917 kb on + strand, within ABZR87_RS05125 at 1080.917 kb on + strand, within ABZR87_RS05125 at 1080.918 kb on - strand, within ABZR87_RS05125 at 1080.918 kb on - strand, within ABZR87_RS05125 at 1080.996 kb on - strand, within ABZR87_RS05125 at 1080.996 kb on - strand, within ABZR87_RS05125 at 1081.106 kb on + strand, within ABZR87_RS05125 at 1081.106 kb on + strand, within ABZR87_RS05125 at 1081.106 kb on + strand, within ABZR87_RS05125 at 1081.107 kb on - strand, within ABZR87_RS05125 at 1081.107 kb on - strand, within ABZR87_RS05125 at 1081.107 kb on - strand, within ABZR87_RS05125 at 1081.107 kb on - strand, within ABZR87_RS05125 at 1081.107 kb on - strand, within ABZR87_RS05125 at 1081.193 kb on + strand at 1081.193 kb on + strand at 1081.193 kb on + strand at 1081.193 kb on + strand at 1081.193 kb on + strand at 1081.193 kb on + strand at 1081.194 kb on - strand at 1081.194 kb on - strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.293 kb on - strand at 1081.293 kb on - strand at 1081.293 kb on - strand at 1081.438 kb on - strand, within ABZR87_RS05130 at 1081.510 kb on + strand at 1081.510 kb on + strand at 1081.510 kb on + strand at 1081.511 kb on - strand at 1081.511 kb on - strand at 1081.511 kb on - strand at 1081.511 kb on - strand at 1081.531 kb on + strand at 1081.531 kb on + strand at 1081.532 kb on - strand at 1081.532 kb on - strand at 1081.532 kb on - strand at 1081.532 kb on - strand at 1081.532 kb on - strand at 1081.532 kb on - strand at 1081.809 kb on - strand, within ABZR87_RS05135 at 1082.126 kb on + strand at 1082.157 kb on + strand at 1082.157 kb on + strand at 1082.158 kb on - strand at 1082.158 kb on - strand at 1082.158 kb on - strand at 1082.310 kb on + strand, within ABZR87_RS05140 at 1082.483 kb on + strand, within ABZR87_RS05140
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,080,304 + +0.1 1,080,305 - -0.5 1,080,305 - +0.5 1,080,355 + -0.2 1,080,356 - -0.2 1,080,356 - +1.0 1,080,372 + -0.2 1,080,372 + -1.5 1,080,373 - +0.8 1,080,373 - +0.4 1,080,373 - +0.6 1,080,373 - -0.1 1,080,388 + -0.7 1,080,388 + -0.1 1,080,389 - -0.3 1,080,389 - -0.8 1,080,495 - -0.6 1,080,580 + -0.4 1,080,580 + +0.9 1,080,580 + -0.4 1,080,580 + -0.4 1,080,581 - -0.7 1,080,581 - -0.9 1,080,581 - -0.1 1,080,716 + ABZR87_RS05125 0.22 +0.4 1,080,716 + ABZR87_RS05125 0.22 +0.4 1,080,716 + ABZR87_RS05125 0.22 -0.9 1,080,716 + ABZR87_RS05125 0.22 -0.3 1,080,717 - ABZR87_RS05125 0.22 +1.1 1,080,749 + ABZR87_RS05125 0.27 -0.7 1,080,749 + ABZR87_RS05125 0.27 +0.1 1,080,749 + ABZR87_RS05125 0.27 +0.7 1,080,750 - ABZR87_RS05125 0.27 +0.2 1,080,750 - ABZR87_RS05125 0.27 -0.4 1,080,750 - ABZR87_RS05125 0.27 +0.1 1,080,750 - ABZR87_RS05125 0.27 +0.1 1,080,827 + ABZR87_RS05125 0.40 -0.4 1,080,827 + ABZR87_RS05125 0.40 -0.5 1,080,827 + ABZR87_RS05125 0.40 -0.4 1,080,827 + ABZR87_RS05125 0.40 -0.6 1,080,828 - ABZR87_RS05125 0.40 +0.9 1,080,828 - ABZR87_RS05125 0.40 -1.0 1,080,917 + ABZR87_RS05125 0.55 +0.1 1,080,917 + ABZR87_RS05125 0.55 +0.3 1,080,918 - ABZR87_RS05125 0.55 +0.0 1,080,918 - ABZR87_RS05125 0.55 -0.2 1,080,996 - ABZR87_RS05125 0.67 -0.0 1,080,996 - ABZR87_RS05125 0.67 +0.1 1,081,106 + ABZR87_RS05125 0.85 -1.8 1,081,106 + ABZR87_RS05125 0.85 -0.7 1,081,106 + ABZR87_RS05125 0.85 -0.2 1,081,107 - ABZR87_RS05125 0.86 -0.0 1,081,107 - ABZR87_RS05125 0.86 -0.4 1,081,107 - ABZR87_RS05125 0.86 -0.0 1,081,107 - ABZR87_RS05125 0.86 -0.7 1,081,107 - ABZR87_RS05125 0.86 -1.0 1,081,193 + +0.2 1,081,193 + -0.4 1,081,193 + -0.2 1,081,193 + +0.4 1,081,193 + +1.0 1,081,193 + +0.9 1,081,194 - +1.0 1,081,194 - +0.2 1,081,292 + -1.7 1,081,292 + +0.9 1,081,292 + -0.3 1,081,292 + +0.1 1,081,292 + -0.1 1,081,292 + -0.4 1,081,292 + -1.4 1,081,292 + -0.8 1,081,292 + -0.7 1,081,292 + -0.0 1,081,293 - +0.2 1,081,293 - -1.7 1,081,293 - -0.7 1,081,438 - ABZR87_RS05130 0.71 -1.3 1,081,510 + +0.6 1,081,510 + +0.2 1,081,510 + -0.6 1,081,511 - +0.8 1,081,511 - -0.5 1,081,511 - -0.3 1,081,511 - +0.9 1,081,531 + +0.1 1,081,531 + -0.2 1,081,532 - +0.6 1,081,532 - +1.6 1,081,532 - -0.5 1,081,532 - +0.2 1,081,532 - +0.1 1,081,532 - +2.6 1,081,809 - ABZR87_RS05135 0.48 -0.4 1,082,126 + -0.4 1,082,157 + +0.1 1,082,157 + -0.2 1,082,158 - -0.0 1,082,158 - +0.6 1,082,158 - -0.1 1,082,310 + ABZR87_RS05140 0.11 -0.4 1,082,483 + ABZR87_RS05140 0.24 -0.4
Or see this region's nucleotide sequence