Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS05010 and ABZR87_RS05015 are separated by 32 nucleotides ABZR87_RS05015 and ABZR87_RS05020 are separated by 42 nucleotides ABZR87_RS05020 and ABZR87_RS05025 are separated by 16 nucleotides
ABZR87_RS05010: ABZR87_RS05010 - rod shape-determining protein RodA, at 1,054,335 to 1,055,477
_RS05010
ABZR87_RS05015: ABZR87_RS05015 - penicillin-binding protein 2, at 1,055,510 to 1,057,912
_RS05015
ABZR87_RS05020: ABZR87_RS05020 - rod shape-determining protein MreD, at 1,057,955 to 1,058,467
_RS05020
ABZR87_RS05025: ABZR87_RS05025 - rod shape-determining protein MreC, at 1,058,484 to 1,059,530
_RS05025
Position (kb)
1055
1056
1057
1058 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1054.591 kb on + strand, within ABZR87_RS05010 at 1054.639 kb on + strand, within ABZR87_RS05010 at 1054.639 kb on + strand, within ABZR87_RS05010 at 1054.640 kb on - strand, within ABZR87_RS05010 at 1054.640 kb on - strand, within ABZR87_RS05010 at 1054.640 kb on - strand, within ABZR87_RS05010 at 1054.805 kb on + strand, within ABZR87_RS05010 at 1054.955 kb on - strand, within ABZR87_RS05010 at 1054.963 kb on + strand, within ABZR87_RS05010 at 1054.964 kb on - strand, within ABZR87_RS05010 at 1055.059 kb on + strand, within ABZR87_RS05010 at 1055.059 kb on + strand, within ABZR87_RS05010 at 1055.060 kb on - strand, within ABZR87_RS05010 at 1055.065 kb on + strand, within ABZR87_RS05010 at 1055.065 kb on + strand, within ABZR87_RS05010 at 1055.098 kb on + strand, within ABZR87_RS05010 at 1055.098 kb on + strand, within ABZR87_RS05010 at 1055.099 kb on - strand, within ABZR87_RS05010 at 1055.239 kb on + strand, within ABZR87_RS05010 at 1055.240 kb on - strand, within ABZR87_RS05010 at 1055.461 kb on - strand at 1055.461 kb on - strand at 1055.508 kb on + strand at 1055.508 kb on + strand at 1055.508 kb on + strand at 1055.508 kb on + strand at 1055.509 kb on - strand at 1055.509 kb on - strand at 1055.509 kb on - strand at 1055.968 kb on + strand, within ABZR87_RS05015 at 1056.039 kb on + strand, within ABZR87_RS05015 at 1056.040 kb on - strand, within ABZR87_RS05015 at 1056.040 kb on - strand, within ABZR87_RS05015 at 1056.042 kb on + strand, within ABZR87_RS05015 at 1056.043 kb on - strand, within ABZR87_RS05015 at 1056.222 kb on + strand, within ABZR87_RS05015 at 1056.222 kb on + strand, within ABZR87_RS05015 at 1056.223 kb on - strand, within ABZR87_RS05015 at 1056.258 kb on + strand, within ABZR87_RS05015 at 1056.259 kb on - strand, within ABZR87_RS05015 at 1056.259 kb on - strand, within ABZR87_RS05015 at 1056.259 kb on - strand, within ABZR87_RS05015 at 1056.361 kb on - strand, within ABZR87_RS05015 at 1056.361 kb on - strand, within ABZR87_RS05015 at 1056.502 kb on - strand, within ABZR87_RS05015 at 1056.540 kb on + strand, within ABZR87_RS05015 at 1056.567 kb on + strand, within ABZR87_RS05015 at 1056.568 kb on - strand, within ABZR87_RS05015 at 1056.711 kb on + strand, within ABZR87_RS05015 at 1056.711 kb on + strand, within ABZR87_RS05015 at 1056.711 kb on + strand, within ABZR87_RS05015 at 1056.711 kb on + strand, within ABZR87_RS05015 at 1056.711 kb on + strand, within ABZR87_RS05015 at 1056.712 kb on - strand, within ABZR87_RS05015 at 1056.712 kb on - strand, within ABZR87_RS05015 at 1056.712 kb on - strand, within ABZR87_RS05015 at 1056.712 kb on - strand, within ABZR87_RS05015 at 1056.712 kb on - strand, within ABZR87_RS05015 at 1056.741 kb on + strand, within ABZR87_RS05015 at 1056.741 kb on + strand, within ABZR87_RS05015 at 1056.741 kb on + strand, within ABZR87_RS05015 at 1056.742 kb on - strand, within ABZR87_RS05015 at 1056.742 kb on - strand, within ABZR87_RS05015 at 1056.742 kb on - strand, within ABZR87_RS05015 at 1056.742 kb on - strand, within ABZR87_RS05015 at 1056.742 kb on - strand, within ABZR87_RS05015 at 1056.742 kb on - strand, within ABZR87_RS05015 at 1056.883 kb on - strand, within ABZR87_RS05015 at 1057.207 kb on - strand, within ABZR87_RS05015 at 1057.237 kb on - strand, within ABZR87_RS05015 at 1057.266 kb on + strand, within ABZR87_RS05015 at 1057.267 kb on - strand, within ABZR87_RS05015 at 1057.281 kb on + strand, within ABZR87_RS05015 at 1057.281 kb on + strand, within ABZR87_RS05015 at 1057.281 kb on + strand, within ABZR87_RS05015 at 1057.281 kb on + strand, within ABZR87_RS05015 at 1057.666 kb on - strand, within ABZR87_RS05015 at 1057.675 kb on - strand at 1057.675 kb on - strand at 1057.675 kb on - strand at 1057.773 kb on + strand at 1057.774 kb on - strand at 1057.930 kb on - strand at 1057.956 kb on + strand at 1057.957 kb on - strand at 1057.957 kb on - strand at 1058.205 kb on + strand, within ABZR87_RS05020 at 1058.221 kb on - strand, within ABZR87_RS05020 at 1058.451 kb on + strand at 1058.452 kb on - strand at 1058.452 kb on - strand at 1058.452 kb on - strand at 1058.452 kb on - strand at 1058.452 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,054,591 + ABZR87_RS05010 0.22 +1.2 1,054,639 + ABZR87_RS05010 0.27 +0.1 1,054,639 + ABZR87_RS05010 0.27 -1.2 1,054,640 - ABZR87_RS05010 0.27 +1.2 1,054,640 - ABZR87_RS05010 0.27 +1.2 1,054,640 - ABZR87_RS05010 0.27 -1.8 1,054,805 + ABZR87_RS05010 0.41 -0.7 1,054,955 - ABZR87_RS05010 0.54 +0.1 1,054,963 + ABZR87_RS05010 0.55 -0.7 1,054,964 - ABZR87_RS05010 0.55 +0.1 1,055,059 + ABZR87_RS05010 0.63 +0.1 1,055,059 + ABZR87_RS05010 0.63 +0.1 1,055,060 - ABZR87_RS05010 0.63 +1.6 1,055,065 + ABZR87_RS05010 0.64 +0.9 1,055,065 + ABZR87_RS05010 0.64 -1.2 1,055,098 + ABZR87_RS05010 0.67 +0.1 1,055,098 + ABZR87_RS05010 0.67 +1.6 1,055,099 - ABZR87_RS05010 0.67 +0.1 1,055,239 + ABZR87_RS05010 0.79 +0.1 1,055,240 - ABZR87_RS05010 0.79 -1.0 1,055,461 - -1.8 1,055,461 - +0.1 1,055,508 + +0.5 1,055,508 + -0.2 1,055,508 + -0.5 1,055,508 + -1.9 1,055,509 - -0.7 1,055,509 - +0.5 1,055,509 - -0.3 1,055,968 + ABZR87_RS05015 0.19 -0.8 1,056,039 + ABZR87_RS05015 0.22 -1.9 1,056,040 - ABZR87_RS05015 0.22 +0.1 1,056,040 - ABZR87_RS05015 0.22 +0.6 1,056,042 + ABZR87_RS05015 0.22 -1.9 1,056,043 - ABZR87_RS05015 0.22 -1.4 1,056,222 + ABZR87_RS05015 0.30 -0.4 1,056,222 + ABZR87_RS05015 0.30 +1.6 1,056,223 - ABZR87_RS05015 0.30 -2.2 1,056,258 + ABZR87_RS05015 0.31 +1.6 1,056,259 - ABZR87_RS05015 0.31 -1.7 1,056,259 - ABZR87_RS05015 0.31 -2.4 1,056,259 - ABZR87_RS05015 0.31 +0.1 1,056,361 - ABZR87_RS05015 0.35 +0.1 1,056,361 - ABZR87_RS05015 0.35 +0.7 1,056,502 - ABZR87_RS05015 0.41 -0.4 1,056,540 + ABZR87_RS05015 0.43 -1.2 1,056,567 + ABZR87_RS05015 0.44 +1.6 1,056,568 - ABZR87_RS05015 0.44 +1.6 1,056,711 + ABZR87_RS05015 0.50 -1.7 1,056,711 + ABZR87_RS05015 0.50 +1.1 1,056,711 + ABZR87_RS05015 0.50 +0.6 1,056,711 + ABZR87_RS05015 0.50 -0.9 1,056,711 + ABZR87_RS05015 0.50 -0.9 1,056,712 - ABZR87_RS05015 0.50 -0.4 1,056,712 - ABZR87_RS05015 0.50 -0.4 1,056,712 - ABZR87_RS05015 0.50 +2.6 1,056,712 - ABZR87_RS05015 0.50 +0.1 1,056,712 - ABZR87_RS05015 0.50 +0.6 1,056,741 + ABZR87_RS05015 0.51 +0.1 1,056,741 + ABZR87_RS05015 0.51 -0.4 1,056,741 + ABZR87_RS05015 0.51 -1.5 1,056,742 - ABZR87_RS05015 0.51 -0.7 1,056,742 - ABZR87_RS05015 0.51 -1.8 1,056,742 - ABZR87_RS05015 0.51 +0.1 1,056,742 - ABZR87_RS05015 0.51 -0.7 1,056,742 - ABZR87_RS05015 0.51 +1.6 1,056,742 - ABZR87_RS05015 0.51 +1.6 1,056,883 - ABZR87_RS05015 0.57 +0.6 1,057,207 - ABZR87_RS05015 0.71 -1.2 1,057,237 - ABZR87_RS05015 0.72 -1.2 1,057,266 + ABZR87_RS05015 0.73 -0.4 1,057,267 - ABZR87_RS05015 0.73 +0.3 1,057,281 + ABZR87_RS05015 0.74 +1.1 1,057,281 + ABZR87_RS05015 0.74 -0.9 1,057,281 + ABZR87_RS05015 0.74 -1.4 1,057,281 + ABZR87_RS05015 0.74 +1.1 1,057,666 - ABZR87_RS05015 0.90 -0.9 1,057,675 - +2.6 1,057,675 - +0.6 1,057,675 - -0.8 1,057,773 + -1.7 1,057,774 - -0.9 1,057,930 - -0.5 1,057,956 + +0.1 1,057,957 - -0.1 1,057,957 - +0.4 1,058,205 + ABZR87_RS05020 0.49 -0.9 1,058,221 - ABZR87_RS05020 0.52 +2.6 1,058,451 + +1.6 1,058,452 - -0.2 1,058,452 - +0.2 1,058,452 - -0.0 1,058,452 - +0.3 1,058,452 - +0.8
Or see this region's nucleotide sequence