Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS04255 and ABZR87_RS04260 are separated by 93 nucleotides ABZR87_RS04260 and ABZR87_RS04265 are separated by 138 nucleotides ABZR87_RS04265 and ABZR87_RS04270 are separated by 60 nucleotides ABZR87_RS04270 and ABZR87_RS04275 are separated by 18 nucleotides ABZR87_RS04275 and ABZR87_RS04280 are separated by 76 nucleotides
ABZR87_RS04255: ABZR87_RS04255 - lysoplasmalogenase, at 889,259 to 890,023
_RS04255
ABZR87_RS04260: ABZR87_RS04260 - Lrp/AsnC family transcriptional regulator, at 890,117 to 890,593
_RS04260
ABZR87_RS04265: ABZR87_RS04265 - phenylalanine 4-monooxygenase, at 890,732 to 891,676
_RS04265
ABZR87_RS04270: ABZR87_RS04270 - 4a-hydroxytetrahydrobiopterin dehydratase, at 891,737 to 892,051
_RS04270
ABZR87_RS04275: ABZR87_RS04275 - DUF3717 domain-containing protein, at 892,070 to 892,297
_RS04275
ABZR87_RS04280: ABZR87_RS04280 - response regulator transcription factor, at 892,374 to 893,084
_RS04280
Position (kb) 890
891
892 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 889.905 kb on + strand, within ABZR87_RS04255 at 889.905 kb on + strand, within ABZR87_RS04255 at 889.905 kb on + strand, within ABZR87_RS04255 at 889.906 kb on - strand, within ABZR87_RS04255 at 889.906 kb on - strand, within ABZR87_RS04255 at 890.054 kb on + strand at 890.054 kb on + strand at 890.054 kb on + strand at 890.055 kb on - strand at 890.055 kb on - strand at 890.118 kb on + strand at 890.118 kb on + strand at 890.118 kb on + strand at 890.118 kb on + strand at 890.118 kb on + strand at 890.119 kb on - strand at 890.119 kb on - strand at 890.119 kb on - strand at 890.171 kb on + strand, within ABZR87_RS04260 at 890.171 kb on + strand, within ABZR87_RS04260 at 890.171 kb on + strand, within ABZR87_RS04260 at 890.171 kb on + strand, within ABZR87_RS04260 at 890.171 kb on + strand, within ABZR87_RS04260 at 890.172 kb on - strand, within ABZR87_RS04260 at 890.172 kb on - strand, within ABZR87_RS04260 at 890.172 kb on - strand, within ABZR87_RS04260 at 890.172 kb on - strand, within ABZR87_RS04260 at 890.172 kb on - strand, within ABZR87_RS04260 at 890.172 kb on - strand, within ABZR87_RS04260 at 890.172 kb on - strand, within ABZR87_RS04260 at 890.172 kb on - strand, within ABZR87_RS04260 at 890.176 kb on + strand, within ABZR87_RS04260 at 890.177 kb on - strand, within ABZR87_RS04260 at 890.274 kb on + strand, within ABZR87_RS04260 at 890.274 kb on + strand, within ABZR87_RS04260 at 890.274 kb on + strand, within ABZR87_RS04260 at 890.274 kb on + strand, within ABZR87_RS04260 at 890.274 kb on + strand, within ABZR87_RS04260 at 890.274 kb on + strand, within ABZR87_RS04260 at 890.274 kb on + strand, within ABZR87_RS04260 at 890.275 kb on - strand, within ABZR87_RS04260 at 890.275 kb on - strand, within ABZR87_RS04260 at 890.275 kb on - strand, within ABZR87_RS04260 at 890.275 kb on - strand, within ABZR87_RS04260 at 890.298 kb on + strand, within ABZR87_RS04260 at 890.299 kb on - strand, within ABZR87_RS04260 at 890.299 kb on - strand, within ABZR87_RS04260 at 890.691 kb on + strand at 890.691 kb on + strand at 890.692 kb on - strand at 890.692 kb on - strand at 890.692 kb on - strand at 890.692 kb on - strand at 890.692 kb on - strand at 890.727 kb on + strand at 890.727 kb on + strand at 890.728 kb on - strand at 890.728 kb on - strand at 890.728 kb on - strand at 890.728 kb on - strand at 890.728 kb on - strand at 890.888 kb on + strand, within ABZR87_RS04265 at 890.889 kb on - strand, within ABZR87_RS04265 at 890.925 kb on - strand, within ABZR87_RS04265 at 890.946 kb on - strand, within ABZR87_RS04265 at 890.947 kb on - strand, within ABZR87_RS04265 at 891.171 kb on - strand, within ABZR87_RS04265 at 891.171 kb on - strand, within ABZR87_RS04265 at 891.171 kb on - strand, within ABZR87_RS04265 at 891.245 kb on + strand, within ABZR87_RS04265 at 891.246 kb on - strand, within ABZR87_RS04265 at 891.246 kb on - strand, within ABZR87_RS04265 at 891.246 kb on - strand, within ABZR87_RS04265 at 891.246 kb on - strand, within ABZR87_RS04265 at 891.317 kb on + strand, within ABZR87_RS04265 at 891.317 kb on + strand, within ABZR87_RS04265 at 891.318 kb on - strand, within ABZR87_RS04265 at 891.318 kb on - strand, within ABZR87_RS04265 at 891.323 kb on + strand, within ABZR87_RS04265 at 891.323 kb on + strand, within ABZR87_RS04265 at 891.323 kb on + strand, within ABZR87_RS04265 at 891.324 kb on - strand, within ABZR87_RS04265 at 891.324 kb on - strand, within ABZR87_RS04265 at 891.324 kb on - strand, within ABZR87_RS04265 at 891.324 kb on - strand, within ABZR87_RS04265 at 891.371 kb on + strand, within ABZR87_RS04265 at 891.371 kb on + strand, within ABZR87_RS04265 at 891.371 kb on + strand, within ABZR87_RS04265 at 891.371 kb on + strand, within ABZR87_RS04265 at 891.372 kb on - strand, within ABZR87_RS04265 at 891.372 kb on - strand, within ABZR87_RS04265 at 891.372 kb on - strand, within ABZR87_RS04265 at 891.411 kb on - strand, within ABZR87_RS04265 at 891.411 kb on - strand, within ABZR87_RS04265 at 891.411 kb on - strand, within ABZR87_RS04265 at 891.503 kb on + strand, within ABZR87_RS04265 at 891.503 kb on + strand, within ABZR87_RS04265 at 891.503 kb on + strand, within ABZR87_RS04265 at 891.504 kb on - strand, within ABZR87_RS04265 at 891.504 kb on - strand, within ABZR87_RS04265 at 891.504 kb on - strand, within ABZR87_RS04265 at 891.504 kb on - strand, within ABZR87_RS04265 at 891.504 kb on - strand, within ABZR87_RS04265 at 891.504 kb on - strand, within ABZR87_RS04265 at 891.504 kb on - strand, within ABZR87_RS04265 at 891.504 kb on - strand, within ABZR87_RS04265 at 891.566 kb on + strand, within ABZR87_RS04265 at 891.567 kb on - strand, within ABZR87_RS04265 at 891.567 kb on - strand, within ABZR87_RS04265 at 891.567 kb on - strand, within ABZR87_RS04265 at 891.567 kb on - strand, within ABZR87_RS04265 at 891.567 kb on - strand, within ABZR87_RS04265 at 891.628 kb on - strand at 891.628 kb on - strand at 891.628 kb on - strand at 891.699 kb on + strand at 891.699 kb on + strand at 891.699 kb on + strand at 891.699 kb on + strand at 891.699 kb on + strand at 891.700 kb on - strand at 891.700 kb on - strand at 891.700 kb on - strand at 891.700 kb on - strand at 891.700 kb on - strand at 891.700 kb on - strand at 891.701 kb on + strand at 891.701 kb on + strand at 891.702 kb on - strand at 891.702 kb on - strand at 891.702 kb on - strand at 891.702 kb on - strand at 891.702 kb on - strand at 891.702 kb on - strand at 891.702 kb on - strand at 891.946 kb on + strand, within ABZR87_RS04270 at 891.946 kb on + strand, within ABZR87_RS04270 at 891.946 kb on + strand, within ABZR87_RS04270 at 891.946 kb on + strand, within ABZR87_RS04270 at 891.946 kb on + strand, within ABZR87_RS04270 at 891.946 kb on + strand, within ABZR87_RS04270 at 891.946 kb on + strand, within ABZR87_RS04270 at 891.946 kb on + strand, within ABZR87_RS04270 at 891.946 kb on + strand, within ABZR87_RS04270 at 891.946 kb on + strand, within ABZR87_RS04270 at 891.947 kb on - strand, within ABZR87_RS04270 at 891.947 kb on - strand, within ABZR87_RS04270 at 891.947 kb on - strand, within ABZR87_RS04270 at 891.947 kb on - strand, within ABZR87_RS04270 at 892.049 kb on + strand at 892.049 kb on + strand at 892.049 kb on + strand at 892.050 kb on - strand at 892.101 kb on + strand, within ABZR87_RS04275 at 892.101 kb on + strand, within ABZR87_RS04275 at 892.101 kb on + strand, within ABZR87_RS04275 at 892.101 kb on + strand, within ABZR87_RS04275 at 892.101 kb on + strand, within ABZR87_RS04275 at 892.102 kb on - strand, within ABZR87_RS04275 at 892.102 kb on - strand, within ABZR87_RS04275 at 892.157 kb on + strand, within ABZR87_RS04275 at 892.157 kb on + strand, within ABZR87_RS04275 at 892.157 kb on + strand, within ABZR87_RS04275 at 892.157 kb on + strand, within ABZR87_RS04275 at 892.157 kb on + strand, within ABZR87_RS04275 at 892.157 kb on + strand, within ABZR87_RS04275 at 892.157 kb on + strand, within ABZR87_RS04275 at 892.157 kb on + strand, within ABZR87_RS04275 at 892.157 kb on + strand, within ABZR87_RS04275 at 892.157 kb on + strand, within ABZR87_RS04275 at 892.158 kb on - strand, within ABZR87_RS04275 at 892.158 kb on - strand, within ABZR87_RS04275 at 892.158 kb on - strand, within ABZR87_RS04275 at 892.158 kb on - strand, within ABZR87_RS04275 at 892.158 kb on - strand, within ABZR87_RS04275 at 892.158 kb on - strand, within ABZR87_RS04275 at 892.173 kb on + strand, within ABZR87_RS04275 at 892.173 kb on + strand, within ABZR87_RS04275 at 892.174 kb on - strand, within ABZR87_RS04275 at 892.174 kb on - strand, within ABZR87_RS04275 at 892.174 kb on - strand, within ABZR87_RS04275 at 892.308 kb on + strand at 892.308 kb on + strand at 892.308 kb on + strand at 892.308 kb on + strand at 892.308 kb on + strand at 892.309 kb on - strand at 892.309 kb on - strand at 892.309 kb on - strand at 892.309 kb on - strand at 892.309 kb on - strand at 892.309 kb on - strand at 892.309 kb on - strand at 892.665 kb on + strand, within ABZR87_RS04280 at 892.666 kb on - strand, within ABZR87_RS04280 at 892.666 kb on - strand, within ABZR87_RS04280 at 892.666 kb on - strand, within ABZR87_RS04280 at 892.666 kb on - strand, within ABZR87_RS04280 at 892.666 kb on - strand, within ABZR87_RS04280 at 892.666 kb on - strand, within ABZR87_RS04280
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 889,905 + ABZR87_RS04255 0.84 -1.8 889,905 + ABZR87_RS04255 0.84 +0.8 889,905 + ABZR87_RS04255 0.84 +0.2 889,906 - ABZR87_RS04255 0.85 -0.4 889,906 - ABZR87_RS04255 0.85 +0.2 890,054 + +0.8 890,054 + +1.6 890,054 + -1.6 890,055 - -0.7 890,055 - -1.9 890,118 + -1.6 890,118 + -0.5 890,118 + -0.2 890,118 + +0.0 890,118 + -0.2 890,119 - +1.3 890,119 - -0.3 890,119 - +0.2 890,171 + ABZR87_RS04260 0.11 +0.2 890,171 + ABZR87_RS04260 0.11 -0.1 890,171 + ABZR87_RS04260 0.11 -0.6 890,171 + ABZR87_RS04260 0.11 -0.4 890,171 + ABZR87_RS04260 0.11 -0.5 890,172 - ABZR87_RS04260 0.12 -0.9 890,172 - ABZR87_RS04260 0.12 +0.7 890,172 - ABZR87_RS04260 0.12 -2.2 890,172 - ABZR87_RS04260 0.12 +0.1 890,172 - ABZR87_RS04260 0.12 -0.7 890,172 - ABZR87_RS04260 0.12 +1.4 890,172 - ABZR87_RS04260 0.12 -2.0 890,172 - ABZR87_RS04260 0.12 -0.8 890,176 + ABZR87_RS04260 0.12 -0.4 890,177 - ABZR87_RS04260 0.13 +0.2 890,274 + ABZR87_RS04260 0.33 -1.5 890,274 + ABZR87_RS04260 0.33 +0.5 890,274 + ABZR87_RS04260 0.33 -0.2 890,274 + ABZR87_RS04260 0.33 -0.1 890,274 + ABZR87_RS04260 0.33 -1.0 890,274 + ABZR87_RS04260 0.33 +0.4 890,274 + ABZR87_RS04260 0.33 +0.3 890,275 - ABZR87_RS04260 0.33 -0.2 890,275 - ABZR87_RS04260 0.33 -0.4 890,275 - ABZR87_RS04260 0.33 +0.1 890,275 - ABZR87_RS04260 0.33 +0.9 890,298 + ABZR87_RS04260 0.38 -0.8 890,299 - ABZR87_RS04260 0.38 +0.4 890,299 - ABZR87_RS04260 0.38 +0.3 890,691 + +2.0 890,691 + -0.7 890,692 - +0.6 890,692 - -0.5 890,692 - -0.1 890,692 - -1.1 890,692 - +0.0 890,727 + -0.4 890,727 + +0.3 890,728 - +0.3 890,728 - -0.1 890,728 - +2.2 890,728 - -0.1 890,728 - -0.9 890,888 + ABZR87_RS04265 0.17 -0.7 890,889 - ABZR87_RS04265 0.17 -1.2 890,925 - ABZR87_RS04265 0.20 -0.4 890,946 - ABZR87_RS04265 0.23 +0.0 890,947 - ABZR87_RS04265 0.23 -1.3 891,171 - ABZR87_RS04265 0.46 +2.0 891,171 - ABZR87_RS04265 0.46 -2.2 891,171 - ABZR87_RS04265 0.46 -1.6 891,245 + ABZR87_RS04265 0.54 +0.3 891,246 - ABZR87_RS04265 0.54 +2.2 891,246 - ABZR87_RS04265 0.54 +0.2 891,246 - ABZR87_RS04265 0.54 +0.4 891,246 - ABZR87_RS04265 0.54 -2.5 891,317 + ABZR87_RS04265 0.62 -1.9 891,317 + ABZR87_RS04265 0.62 +2.6 891,318 - ABZR87_RS04265 0.62 -0.7 891,318 - ABZR87_RS04265 0.62 +1.0 891,323 + ABZR87_RS04265 0.63 -0.4 891,323 + ABZR87_RS04265 0.63 -1.0 891,323 + ABZR87_RS04265 0.63 -1.9 891,324 - ABZR87_RS04265 0.63 -0.6 891,324 - ABZR87_RS04265 0.63 -1.6 891,324 - ABZR87_RS04265 0.63 -0.3 891,324 - ABZR87_RS04265 0.63 +0.0 891,371 + ABZR87_RS04265 0.68 +0.0 891,371 + ABZR87_RS04265 0.68 +0.1 891,371 + ABZR87_RS04265 0.68 -0.7 891,371 + ABZR87_RS04265 0.68 +0.4 891,372 - ABZR87_RS04265 0.68 -0.2 891,372 - ABZR87_RS04265 0.68 +0.3 891,372 - ABZR87_RS04265 0.68 +0.3 891,411 - ABZR87_RS04265 0.72 -0.5 891,411 - ABZR87_RS04265 0.72 +0.3 891,411 - ABZR87_RS04265 0.72 -1.2 891,503 + ABZR87_RS04265 0.82 +1.8 891,503 + ABZR87_RS04265 0.82 -0.4 891,503 + ABZR87_RS04265 0.82 -1.4 891,504 - ABZR87_RS04265 0.82 -0.2 891,504 - ABZR87_RS04265 0.82 -1.5 891,504 - ABZR87_RS04265 0.82 +1.4 891,504 - ABZR87_RS04265 0.82 +0.5 891,504 - ABZR87_RS04265 0.82 -0.9 891,504 - ABZR87_RS04265 0.82 +0.6 891,504 - ABZR87_RS04265 0.82 -2.6 891,504 - ABZR87_RS04265 0.82 -2.1 891,566 + ABZR87_RS04265 0.88 +0.2 891,567 - ABZR87_RS04265 0.88 -0.3 891,567 - ABZR87_RS04265 0.88 -0.4 891,567 - ABZR87_RS04265 0.88 -0.7 891,567 - ABZR87_RS04265 0.88 -0.3 891,567 - ABZR87_RS04265 0.88 +0.9 891,628 - +0.1 891,628 - -0.6 891,628 - -0.7 891,699 + -0.2 891,699 + -0.1 891,699 + -0.2 891,699 + +0.1 891,699 + +0.9 891,700 - -0.5 891,700 - -0.1 891,700 - +0.0 891,700 - -1.6 891,700 - +0.0 891,700 - -1.1 891,701 + -2.4 891,701 + -0.2 891,702 - -0.0 891,702 - -1.3 891,702 - +0.1 891,702 - -0.5 891,702 - -0.0 891,702 - -0.5 891,702 - +0.3 891,946 + ABZR87_RS04270 0.66 +0.6 891,946 + ABZR87_RS04270 0.66 +0.6 891,946 + ABZR87_RS04270 0.66 -0.0 891,946 + ABZR87_RS04270 0.66 -0.1 891,946 + ABZR87_RS04270 0.66 +0.0 891,946 + ABZR87_RS04270 0.66 +0.5 891,946 + ABZR87_RS04270 0.66 +0.8 891,946 + ABZR87_RS04270 0.66 -0.2 891,946 + ABZR87_RS04270 0.66 +0.1 891,946 + ABZR87_RS04270 0.66 -0.9 891,947 - ABZR87_RS04270 0.67 -1.0 891,947 - ABZR87_RS04270 0.67 +0.5 891,947 - ABZR87_RS04270 0.67 -3.0 891,947 - ABZR87_RS04270 0.67 +0.0 892,049 + -0.1 892,049 + +0.4 892,049 + -0.9 892,050 - -0.4 892,101 + ABZR87_RS04275 0.14 -0.2 892,101 + ABZR87_RS04275 0.14 +0.8 892,101 + ABZR87_RS04275 0.14 +0.3 892,101 + ABZR87_RS04275 0.14 +1.0 892,101 + ABZR87_RS04275 0.14 +0.9 892,102 - ABZR87_RS04275 0.14 +0.3 892,102 - ABZR87_RS04275 0.14 -0.2 892,157 + ABZR87_RS04275 0.38 -0.6 892,157 + ABZR87_RS04275 0.38 -1.0 892,157 + ABZR87_RS04275 0.38 -0.3 892,157 + ABZR87_RS04275 0.38 -1.2 892,157 + ABZR87_RS04275 0.38 +0.2 892,157 + ABZR87_RS04275 0.38 +0.1 892,157 + ABZR87_RS04275 0.38 +0.8 892,157 + ABZR87_RS04275 0.38 +0.3 892,157 + ABZR87_RS04275 0.38 -0.1 892,157 + ABZR87_RS04275 0.38 -1.9 892,158 - ABZR87_RS04275 0.39 -0.5 892,158 - ABZR87_RS04275 0.39 -0.7 892,158 - ABZR87_RS04275 0.39 -0.3 892,158 - ABZR87_RS04275 0.39 +0.0 892,158 - ABZR87_RS04275 0.39 -0.0 892,158 - ABZR87_RS04275 0.39 -0.4 892,173 + ABZR87_RS04275 0.45 -0.1 892,173 + ABZR87_RS04275 0.45 -0.7 892,174 - ABZR87_RS04275 0.46 -1.3 892,174 - ABZR87_RS04275 0.46 -0.2 892,174 - ABZR87_RS04275 0.46 -1.0 892,308 + +0.9 892,308 + -0.1 892,308 + -0.9 892,308 + +0.2 892,308 + -0.9 892,309 - -0.2 892,309 - -1.6 892,309 - +0.6 892,309 - +1.1 892,309 - -1.2 892,309 - -0.2 892,309 - +0.3 892,665 + ABZR87_RS04280 0.41 +1.0 892,666 - ABZR87_RS04280 0.41 -1.0 892,666 - ABZR87_RS04280 0.41 -1.6 892,666 - ABZR87_RS04280 0.41 -0.1 892,666 - ABZR87_RS04280 0.41 +1.3 892,666 - ABZR87_RS04280 0.41 +0.4 892,666 - ABZR87_RS04280 0.41 +0.8
Or see this region's nucleotide sequence